##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105428
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976922639147094	33.0	31.0	34.0	30.0	34.0
2	32.24470728838639	34.0	31.0	34.0	30.0	34.0
3	32.23513677580908	33.0	31.0	34.0	30.0	34.0
4	35.876370603634705	37.0	35.0	37.0	35.0	37.0
5	35.98455818188717	37.0	35.0	37.0	35.0	37.0
6	36.006117919338315	37.0	35.0	37.0	35.0	37.0
7	35.96682095837918	37.0	35.0	37.0	35.0	37.0
8	35.81997192396707	37.0	35.0	37.0	35.0	37.0
9	37.65079485525667	39.0	38.0	39.0	35.0	39.0
10	37.3000436316728	39.0	37.0	39.0	34.0	39.0
11	37.312431232689605	39.0	37.0	39.0	34.0	39.0
12	37.321318814736124	39.0	37.0	39.0	34.0	39.0
13	37.263459422544294	39.0	37.0	39.0	34.0	39.0
14	38.45276397162044	40.0	38.0	41.0	34.0	41.0
15	38.48399855825777	40.0	38.0	41.0	34.0	41.0
16	38.59684334332435	40.0	38.0	41.0	34.0	41.0
17	38.60077019387639	40.0	38.0	41.0	34.0	41.0
18	38.02886330007209	39.0	38.0	40.0	34.0	41.0
19	37.36824183328907	37.0	37.0	40.0	34.0	41.0
20	36.4771407975111	35.0	35.0	40.0	34.0	41.0
21	36.380060325530216	35.0	35.0	40.0	33.0	41.0
22	36.375346207838525	35.0	35.0	40.0	33.0	41.0
23	36.28279015062412	35.0	35.0	40.0	33.0	41.0
24	36.11764426907463	35.0	35.0	40.0	33.0	41.0
25	36.017206055317374	35.0	35.0	40.0	33.0	41.0
26	36.02083886633532	35.0	35.0	40.0	33.0	41.0
27	35.96420305801116	35.0	35.0	40.0	33.0	41.0
28	35.90127859771598	35.0	35.0	40.0	33.0	41.0
29	35.657586219979514	35.0	35.0	40.0	32.0	41.0
30	35.28643244678833	35.0	35.0	40.0	30.0	41.0
31	34.67222180066017	35.0	35.0	40.0	25.0	41.0
32	33.92475433471184	35.0	34.0	40.0	21.0	41.0
33	32.88853056114125	35.0	33.0	40.0	15.0	41.0
34	32.03990401031984	35.0	31.0	40.0	10.0	41.0
35	31.524585499108397	35.0	30.0	40.0	8.0	41.0
36	31.19450240922715	35.0	29.0	40.0	7.0	41.0
37	30.964506582691506	35.0	26.0	40.0	7.0	41.0
38	30.743597526273856	35.0	25.0	40.0	7.0	41.0
39	30.66194938725955	35.0	24.0	40.0	7.0	41.0
40	30.555592442235458	35.0	23.0	40.0	7.0	41.0
41	30.427903403270477	35.0	23.0	40.0	7.0	41.0
42	30.139052244185606	35.0	22.0	40.0	7.0	41.0
43	29.892428956254506	35.0	22.0	40.0	7.0	41.0
44	29.635390977728875	35.0	21.0	39.0	7.0	41.0
45	29.618289259020376	35.0	20.0	39.0	7.0	41.0
46	29.307726600144175	35.0	20.0	39.0	7.0	41.0
47	29.23263269719619	35.0	20.0	39.0	7.0	41.0
48	29.127347573699588	35.0	20.0	39.0	7.0	41.0
49	29.129225632659256	35.0	20.0	39.0	7.0	41.0
50	28.897664756990554	35.0	20.0	39.0	7.0	41.0
51	28.125725613688964	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	3.0
13	6.0
14	3.0
15	9.0
16	9.0
17	25.0
18	56.0
19	101.0
20	236.0
21	360.0
22	622.0
23	1046.0
24	1851.0
25	3457.0
26	5254.0
27	5321.0
28	4527.0
29	3978.0
30	3692.0
31	3563.0
32	3628.0
33	3983.0
34	5596.0
35	8796.0
36	10919.0
37	9338.0
38	15743.0
39	13298.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.784231892855788	3.6660090298592407	30.94339264711462	49.606366430170354
2	52.881587434078234	3.745684258451265	30.06222255947187	13.310505747998636
3	13.012672155404637	4.248397010281899	70.12368630724286	12.615244527070606
4	10.745722199036308	3.8576089843305383	69.67124483059528	15.725423986037864
5	8.630534582843268	4.965474067610122	71.22396327351368	15.180028076032933
6	11.774860568349963	5.474826421823424	72.99863413893843	9.751678870888188
7	44.65417156732557	2.297302424403384	48.59430132412641	4.45422468414463
8	42.73153242022992	2.7819933983382024	48.271806351253936	6.214667830177941
9	39.38801836324316	4.431460333118337	50.56626323177903	5.614258071859468
10	20.219486284478506	15.094661759684334	53.96668816633152	10.719163789505634
11	13.454679971165156	13.102781044883713	57.36142201312744	16.08111697082369
12	12.494783169556474	10.776074667071365	61.402094320294424	15.32704784307774
13	14.60902227112342	12.767955381871987	62.39234358993816	10.230678757066434
14	12.419850514094927	14.70292521910688	60.16238570398755	12.714838562810638
15	8.191372310961036	13.412945327616951	63.698448230071705	14.697234131350307
16	8.045301058542323	13.533406685131085	60.998027089577725	17.423265166748873
17	7.978905034715636	13.44709185415639	58.9406988655765	19.633304245551468
18	8.74720188185302	12.503319801191335	62.442614865121215	16.306863451834428
19	9.615092764730433	14.12053723868422	62.32879310998975	13.935576886595591
20	9.819023409340973	17.2895246044694	60.18040748188337	12.711044504306257
21	9.021322608794629	18.097659065902796	60.317942102667224	12.563076222635353
22	7.457221990363092	17.146298895928975	58.16386538680427	17.23261372690367
23	7.956140683689343	17.2895246044694	56.46886974997154	18.285464961869714
24	9.293546306484046	16.044124900405965	56.38445194824904	18.27787684486095
25	8.308039609970786	17.815001707326328	54.61642068520697	19.260537997495923
26	8.543271237242479	18.110938270668132	56.569412300337675	16.776378191751718
27	9.699510566452934	17.161475129946506	58.014000075881164	15.12501422771939
28	7.754107068330994	15.408620100921958	58.676063284895854	18.161209545851197
29	10.616724209887316	14.261865917972456	56.94407557764541	18.17733429449482
30	14.161323367606329	15.362142884243275	54.98728990401032	15.489243844140077
31	12.438820806616837	19.705391357134726	50.922904731190954	16.93288310505748
32	12.392343589938157	20.453769397124102	49.00880221573017	18.145084797207574
33	14.069317448875063	16.982205865614446	47.78616686269302	21.16230982281747
34	14.474333194217856	16.728003945820845	47.107030390408624	21.690632469552682
35	16.59710892741966	17.7988769586827	43.99400538756308	21.61000872633456
36	17.598740372576547	17.560799787532723	44.898888340858214	19.941571499032516
37	13.504002731722123	19.65512008195166	47.43901050954206	19.401866676784156
38	13.010775126152446	22.84592328413704	47.67993322457033	16.46336836514019
39	15.844936828925901	20.044011078650833	46.99605417915544	17.114997913267825
40	15.596425996888872	16.461471335888	47.39822438061994	20.543878286603178
41	15.745342793185872	16.672990097507302	44.76514777857874	22.81651933072808
42	20.255529840270135	16.126645672876275	45.661494100239025	17.95633038661456
43	18.68384110482984	16.70998216792503	46.74469780324013	17.86147892400501
44	15.198998368554841	17.52570474636719	45.29631596919225	21.978980915885725
45	17.33695033577418	15.539515119323141	44.277611260765646	22.84592328413704
46	18.168797662859962	15.94737640854422	47.54998672079523	18.333839207800583
47	13.144515688431916	18.241833289069316	50.442956330386615	18.170694692112153
48	13.643434381758166	17.16052661532041	50.37086921880335	18.82516978411807
49	18.124217475433472	13.637743294001595	50.74742952536329	17.490609705201653
50	16.39317828280912	12.238684220510681	47.509200591873125	23.85893690480707
51	13.983951132526466	12.26429411541526	45.03452593238988	28.7172288196684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	440.0
2	634.0
3	9330.5
4	18027.0
5	10935.5
6	3844.0
7	3436.5
8	3029.0
9	2953.0
10	2877.0
11	2762.5
12	2648.0
13	2497.5
14	2347.0
15	2193.0
16	2039.0
17	1832.0
18	1625.0
19	1502.5
20	1380.0
21	1228.5
22	1077.0
23	973.0
24	869.0
25	813.5
26	712.5
27	667.0
28	661.5
29	656.0
30	703.0
31	750.0
32	798.0
33	846.0
34	855.5
35	865.0
36	1016.5
37	1168.0
38	1317.0
39	1466.0
40	2080.0
41	2694.0
42	3511.5
43	4329.0
44	4822.0
45	5315.0
46	7009.5
47	8704.0
48	10264.5
49	11825.0
50	11192.5
51	10560.0
52	8305.0
53	6050.0
54	4440.0
55	2830.0
56	2153.0
57	1476.0
58	1220.0
59	964.0
60	917.0
61	870.0
62	778.5
63	687.0
64	588.0
65	489.0
66	400.5
67	312.0
68	237.0
69	162.0
70	146.5
71	131.0
72	108.5
73	86.0
74	72.5
75	43.0
76	27.0
77	23.0
78	19.0
79	13.5
80	8.0
81	9.0
82	10.0
83	5.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	105428.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.718177334294495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4510717943285	43.754031187160905
2	9.58788007204153	10.300868839397504
3	3.8581064378288663	6.217513374056228
4	1.7833810078751282	3.831999089425959
5	0.9464279408129392	2.542019197936032
6	0.550905816294099	1.7756193800508402
7	0.34608185895398524	1.3013620670030732
8	0.22248119504184766	0.9561027431042988
9	0.16068086308577886	0.7768334787722426
>10	0.9905710350672741	9.839890731115073
>50	0.06709750326658898	2.3485222142125433
>100	0.026485856552600915	2.422506355047995
>500	0.005297171310520182	1.5887619987100203
>1k	0.001765723770173394	1.0566452934704254
>5k	0.0	0.0
>10k+	0.001765723770173394	11.28732405053686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11900	11.28732405053686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	1114	1.0566452934704254	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	640	0.607049360701142	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	519	0.49227909094358235	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	516	0.48943354706529574	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	438	0.41544940622984405	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	287	0.2722236976894184	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	209	0.1982395568539667	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	192	0.18211480821034262	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	161	0.15271085480138105	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	148	0.1403801646621391	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.1346890769055659	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.1299465037750882	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	136	0.12899798914899266	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTTT	127	0.12046135751413287	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	124	0.11761581363584625	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	116	0.11002769662708199	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATG	113	0.10718215274879539	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	113	0.10718215274879539	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	111	0.10528512349660432	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5254771028569261	0.0
2	0.0	0.0	0.0	1.8116629358424707	0.0
3	0.0	0.0	0.0	2.554349888075274	0.0
4	0.0	0.0	0.0	3.5597753917365407	0.0
5	0.0	0.0	0.0	5.591493720833175	0.0
6	0.0	0.0	0.0	6.943127063019312	0.0
7	0.0	0.0	0.0	8.424706908980536	0.0
8	0.0	0.0	0.0	10.77322912319308	0.0
9	0.0	0.0	0.0	11.655347725461926	0.0
10	0.0	0.0	0.0	13.997230337291802	0.0
11	0.0	0.0	0.0	19.54604089995068	0.0
12	0.0	0.0	0.0	24.390105095420573	0.0
13	0.0	0.0	0.0	25.93238987745191	0.0
14	0.0	0.0	0.0	26.47399172895246	0.0
15	0.0	0.0	0.0	27.308684599916532	0.0
16	0.0	0.0	0.0	29.085252494593465	0.0
17	0.0	0.0	0.0	31.33987176082255	0.0
18	0.0	0.0	0.0	33.50343362294647	0.0
19	0.0	0.0	0.0	34.92146298895929	0.0
20	0.0	0.0	0.0	36.09572409606556	0.0
21	0.0	0.0	0.0	37.419850514094925	0.0
22	0.0	0.0	0.0	38.56375915316614	0.0
23	0.0	0.0	0.0	39.41267974352164	0.0
24	0.0	0.0	0.0	39.85279053002997	0.0
25	0.0	0.0	0.0	40.168645900519785	0.0
26	0.0	0.0	0.0	40.42379633493948	0.0
27	0.0	0.0	0.0	40.657130932958985	0.0
28	0.0	0.0	0.0	40.842091285047616	0.0
29	0.0	0.0	0.0	41.020412034753576	0.0
30	0.0	0.0	0.0	41.20537238684221	0.0
31	0.0	0.0	0.0	41.35049512463482	0.0
32	0.0	0.0	0.0	41.48044162840991	0.0
33	0.0	0.0	0.0	41.645483173350534	0.0
34	0.0	0.0	0.0	41.76025344310809	0.0
35	0.0	0.0	0.0	41.90158212239633	0.0
36	0.0	0.0	0.0	42.033425655423606	0.0
37	0.0	0.0	0.0	42.16906324695527	0.0
38	0.0	0.0	0.0	42.303752323860834	0.0
39	0.0	0.0	0.0	42.460257237166594	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCTG	20	7.00413E-4	45.0	1
GGGCACA	20	7.00413E-4	45.0	7
GGACCGA	25	3.8666585E-5	45.0	8
CTCTCGG	20	7.00413E-4	45.0	2
TGTTCGG	20	7.00413E-4	45.0	2
TGCTTTG	25	3.8666585E-5	45.0	1
CGGGATT	20	7.00413E-4	45.0	6
CGGGAGA	25	3.8666585E-5	45.0	6
GACCGAT	25	3.8666585E-5	45.0	9
CGGGACC	25	3.8666585E-5	45.0	6
CGGGAAT	20	7.00413E-4	45.0	6
TGTAGGG	25	3.8666585E-5	45.0	3
GGCCACC	25	3.8666585E-5	45.0	8
GATTTGT	20	7.00413E-4	45.0	10
GGGACCG	25	3.8666585E-5	45.0	7
TGGGCTT	25	3.8666585E-5	45.0	6
TGGGTGT	35	1.1979319E-7	45.0	6
TGGGCAC	25	3.8666585E-5	45.0	6
CCGGGAT	20	7.00413E-4	45.0	5
GGGAAGT	20	7.00413E-4	45.0	7
>>END_MODULE