FastQCFastQC Report
Sat 14 Jan 2017
SRR2933574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81236
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62717.7194839726229745No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC6050.7447437096853612No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC3600.443153281796248Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT3480.42838150573637307Illumina PCR Primer Index 7 (96% over 28bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC3080.3791422522034566No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC3050.3754493081884879No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG2500.3077453345807278No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC2240.27573981978433204Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC2200.2708158944310404Illumina PCR Primer Index 7 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC2020.24865823034122803Illumina PCR Primer Index 7 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.16248953665862426No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1150.14156285390713477No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG1080.13294598453887438No Hit
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG1020.12556009650893693Illumina PCR Primer Index 7 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG980.12063617115564529Illumina PCR Primer Index 7 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.11448126446403073No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT840.10340243241912452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGG302.1405867E-645.0000042
AGCTTGG206.994581E-445.02
TGTCTTG253.8587594E-545.01
GGACCGA206.994581E-445.08
CCTCGGG253.8587594E-545.03
TACGGGA206.994581E-445.04
TCGCGGG253.8587594E-545.03
ATGAATG206.994581E-445.021
TATTGGG253.8587594E-545.03
AAGGGCT206.994581E-445.05
ACCTGGG206.994581E-445.03
CTTGGGT351.1933844E-745.04
TCTGCGG253.8587594E-545.02
GACCGAT206.994581E-445.09
CGGGAAC253.8587594E-545.06
GGGACCG253.8587594E-545.07
CTTGCGG253.8587594E-545.02
TGGGCTA206.994581E-445.06
GGCTAGG206.994581E-445.02
CTGCTTG206.994581E-445.01
Produced by FastQC (version 0.11.5)