##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933574.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81236
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08497464178443	33.0	31.0	34.0	30.0	34.0
2	32.289588359840465	34.0	31.0	34.0	30.0	34.0
3	32.32441282190162	33.0	31.0	34.0	30.0	34.0
4	35.95186862967157	37.0	35.0	37.0	35.0	37.0
5	35.98088285981584	37.0	35.0	37.0	35.0	37.0
6	35.947289379093014	37.0	35.0	37.0	35.0	37.0
7	35.998005810231916	37.0	35.0	37.0	35.0	37.0
8	35.77609680437244	37.0	35.0	37.0	35.0	37.0
9	37.62911763257669	39.0	37.0	39.0	35.0	39.0
10	37.23139987197794	39.0	37.0	39.0	34.0	39.0
11	37.23889654832833	39.0	37.0	39.0	34.0	39.0
12	37.25888768526269	39.0	37.0	39.0	34.0	39.0
13	37.18882022748535	39.0	37.0	39.0	33.0	39.0
14	38.331454035156824	40.0	38.0	41.0	33.0	41.0
15	38.36099758727658	40.0	38.0	41.0	33.0	41.0
16	38.43397016101236	40.0	38.0	41.0	34.0	41.0
17	38.39903491063075	40.0	38.0	41.0	34.0	41.0
18	37.93905411393963	39.0	38.0	40.0	34.0	41.0
19	37.438155497562654	38.0	37.0	40.0	34.0	41.0
20	36.741567777832486	37.0	35.0	40.0	33.0	41.0
21	36.6299177704466	37.0	35.0	40.0	33.0	41.0
22	36.642412231030576	37.0	35.0	40.0	33.0	41.0
23	36.59797380471712	37.0	35.0	40.0	33.0	41.0
24	36.5096139642523	37.0	35.0	40.0	33.0	41.0
25	36.40386035747698	37.0	35.0	40.0	33.0	41.0
26	36.30221822837166	37.0	35.0	40.0	33.0	41.0
27	36.204699886749715	37.0	35.0	40.0	32.0	41.0
28	36.04882071987789	37.0	35.0	40.0	32.0	41.0
29	35.94214387709882	37.0	35.0	40.0	31.0	41.0
30	35.688980255059334	37.0	35.0	40.0	31.0	41.0
31	35.14136589689301	36.0	35.0	40.0	28.0	41.0
32	34.44232852429957	36.0	34.0	40.0	23.0	41.0
33	33.746134718597666	37.0	33.0	40.0	18.0	41.0
34	33.063235511349646	36.0	33.0	40.0	14.0	41.0
35	32.60281156137673	36.0	32.0	40.0	10.0	41.0
36	32.2733394061746	36.0	31.0	40.0	10.0	41.0
37	32.02534590575607	36.0	31.0	40.0	9.0	41.0
38	31.910495346890542	36.0	31.0	40.0	8.0	41.0
39	31.87675414840711	36.0	30.0	40.0	8.0	41.0
40	31.604436456743315	35.0	30.0	40.0	8.0	41.0
41	31.41818898025506	35.0	29.0	40.0	8.0	41.0
42	31.34660741543158	35.0	29.0	40.0	8.0	41.0
43	31.154562016839826	35.0	27.0	40.0	7.0	41.0
44	30.950846915160767	35.0	26.0	40.0	7.0	41.0
45	30.931101974494066	35.0	26.0	40.0	7.0	41.0
46	30.599167856615292	35.0	24.0	40.0	7.0	41.0
47	30.381813481707617	35.0	24.0	39.0	7.0	41.0
48	30.408919690777488	35.0	24.0	39.0	7.0	41.0
49	30.486754640799646	35.0	24.0	39.0	7.0	41.0
50	30.376507952139445	35.0	24.0	39.0	7.0	41.0
51	29.32513910089123	35.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	1.0
14	2.0
15	3.0
16	12.0
17	14.0
18	30.0
19	79.0
20	158.0
21	248.0
22	390.0
23	636.0
24	1173.0
25	2145.0
26	3173.0
27	3513.0
28	3098.0
29	2677.0
30	2526.0
31	2527.0
32	2757.0
33	3178.0
34	4574.0
35	6813.0
36	8294.0
37	9034.0
38	14064.0
39	10111.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.4450243734305	4.425377911270865	35.12728347038259	42.00231424491604
2	45.970998079669116	6.145058840907971	33.722733763356146	14.16120931606677
3	15.411886355802846	5.929637106701462	62.752966665025355	15.905509872470333
4	12.472302919887735	4.502929735585209	65.47097346004234	17.553793884484712
5	12.026687675414841	5.541877985129746	64.61790339258457	17.813530946870845
6	13.665123836722634	7.353882515141071	67.82460977891576	11.156383869220543
7	53.024521148259396	2.3302476734452706	40.00689349549461	4.638337682800729
8	51.67905854547246	3.9034418238219506	38.42877541976464	5.988724210940962
9	47.635284849081685	4.579250578561229	40.512826825545325	7.272637746811758
10	25.249889211679548	17.039243685065735	45.09700132945984	12.613865773794869
11	17.103254714658526	14.811167462701267	49.20724801812005	18.878329804520163
12	14.404943621054706	12.70249643015412	54.62110394406421	18.271456004726968
13	17.161110837559704	14.492343296075633	55.61450588409079	12.732039982273868
14	15.14353242404845	16.91491456989512	53.502141907528674	14.439411098527746
15	10.810478113151804	14.638830075336056	56.80609581958738	17.744595991924765
16	10.42025702890344	15.001969570141316	54.01423014427101	20.563543256684227
17	10.116204638337683	14.935496577871879	52.58136786646315	22.366930917327295
18	11.068984194199615	13.985178984686591	55.40031513122261	19.54552168989118
19	12.192870156088434	15.823034122802698	55.56772859323452	16.41636712787434
20	12.860062041459452	18.901718449948298	53.26825545324733	14.96996405534492
21	11.732483135555665	20.48722241370821	52.91496380914865	14.865330641587473
22	10.031266925993403	18.346545866364668	51.76030331380176	19.86188389384017
23	10.358707961987296	19.487665566990003	48.80348613915013	21.35014033187257
24	11.654931311241322	18.436407504062238	49.735339012260575	20.173322172435864
25	10.374710719385494	21.128563690974445	47.33984932788419	21.156876261755873
26	10.324240484514254	21.256585750160028	49.15554680191048	19.263626963415234
27	12.532621005465558	20.582007976759073	49.98030429858683	16.90506671918854
28	9.664434487173175	19.27470579546014	51.03279334285292	20.028066374513763
29	12.670490915357725	17.99202324092767	48.78625240041361	20.551233443301
30	17.97602048352947	19.610763700822297	46.081786400118176	16.33142941553006
31	16.70687872371855	25.25358215569452	40.4685114973657	17.571027623221234
32	15.649465754099168	24.84366537003299	39.61913437392289	19.88773450194495
33	18.369934511792803	19.964055344920972	37.71973016889064	23.946279974395587
34	16.33142941553006	23.180609581958738	37.761583534393615	22.726377468117583
35	16.92722438327835	23.881037963464475	36.58599635629523	22.605741296961938
36	20.11177310551972	26.84647200748437	34.551184204047466	18.490570682948444
37	20.42074942143877	27.284701363927322	35.46334137574475	16.831207838889163
38	20.36904820522921	25.4136097296765	34.23728396277512	19.980058102319166
39	20.833128169776945	21.543404401989267	33.60086661086218	24.022600817371607
40	17.564872716529617	24.117386380422474	36.20931606676842	22.108424836279482
41	15.659313604805753	25.68073268009257	36.93559505637894	21.724358658722736
42	17.552562903146388	21.95824511300409	38.46324289723768	22.025949086611845
43	18.83893840169383	20.537692648579448	38.038554335516274	22.58481461421045
44	18.45733418681373	20.516765965827958	36.534295140085675	24.49160470727264
45	21.529863607267714	19.120833128169775	34.99187552316707	24.35742774139544
46	21.69481510660298	20.1376237136245	39.10704613718056	19.060515042591955
47	14.667142646117485	23.07597616820129	42.49224481756856	19.764636368112658
48	15.112757890590379	20.903294106061352	42.76429169333793	21.21965631001034
49	18.880791767196808	15.81934117878773	44.172534344379336	21.12733270963612
50	18.473336944211926	14.971195036683243	40.512826825545325	26.042641193559508
51	15.906740853808657	14.757004283815057	38.65527598601605	30.680978876360232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	167.0
1	351.5
2	536.0
3	5096.0
4	9656.0
5	5888.5
6	2121.0
7	2037.0
8	1953.0
9	1896.5
10	1840.0
11	1785.5
12	1731.0
13	1585.5
14	1440.0
15	1314.5
16	1189.0
17	1109.0
18	1029.0
19	933.5
20	838.0
21	754.5
22	671.0
23	630.5
24	590.0
25	566.0
26	556.5
27	571.0
28	516.0
29	461.0
30	497.0
31	533.0
32	578.0
33	623.0
34	738.0
35	853.0
36	927.5
37	1002.0
38	1121.0
39	1240.0
40	1590.0
41	1940.0
42	2628.0
43	3316.0
44	3743.0
45	4170.0
46	5863.5
47	7557.0
48	8533.0
49	9509.0
50	9188.0
51	8867.0
52	7269.0
53	5671.0
54	4302.5
55	2934.0
56	2344.0
57	1754.0
58	1582.0
59	1410.0
60	1277.0
61	1144.0
62	1017.0
63	890.0
64	832.0
65	774.0
66	646.5
67	519.0
68	457.0
69	395.0
70	322.0
71	249.0
72	217.5
73	186.0
74	174.0
75	130.5
76	99.0
77	76.5
78	54.0
79	43.0
80	32.0
81	19.5
82	7.0
83	6.0
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	81236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.891821359988185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8294669246635	46.21473238465705
2	10.648748644453422	12.329509084642277
3	4.403665823215463	7.648087055000246
4	1.9817559378255971	4.589098429267812
5	1.0057624019222182	2.9112708651336847
6	0.5996300155223373	2.0828204244423656
7	0.3295838737800081	1.3356147520803585
8	0.21476110485019884	0.9946329213649121
9	0.13821259223032598	0.720124082918903
>10	0.7782432116353739	7.847506031808557
>50	0.04040060388271067	1.5707321877000344
>100	0.02551617087329095	3.291644098675464
>500	0.002126347572774246	0.7447437096853612
>1k	0.0	0.0
>5k	0.002126347572774246	7.7194839726229745
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6271	7.7194839726229745	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	605	0.7447437096853612	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	360	0.443153281796248	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	348	0.42838150573637307	Illumina PCR Primer Index 7 (96% over 28bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	308	0.3791422522034566	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	305	0.3754493081884879	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	250	0.3077453345807278	No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	224	0.27573981978433204	Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	220	0.2708158944310404	Illumina PCR Primer Index 7 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	202	0.24865823034122803	Illumina PCR Primer Index 7 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.16248953665862426	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	115	0.14156285390713477	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	108	0.13294598453887438	No Hit
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	102	0.12556009650893693	Illumina PCR Primer Index 7 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	98	0.12063617115564529	Illumina PCR Primer Index 7 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.11448126446403073	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	84	0.10340243241912452	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46284898320941453	0.0
2	0.0	0.0	0.0	1.591658870451524	0.0
3	0.0	0.0	0.0	2.256388793145896	0.0
4	0.0	0.0	0.0	3.2251711064060267	0.0
5	0.0	0.0	0.0	5.431089664680684	0.0
6	0.0	0.0	0.0	6.8836476439017185	0.0
7	0.0	0.0	0.0	8.379289969964056	0.0
8	0.0	0.0	0.0	10.612290117681816	0.0
9	0.0	0.0	0.0	11.622925796444926	0.0
10	0.0	0.0	0.0	13.953173469890196	0.0
11	0.0	0.0	0.0	19.44335023881038	0.0
12	0.0	0.0	0.0	24.612240878428285	0.0
13	0.0	0.0	0.0	26.093111428430745	0.0
14	0.0	0.0	0.0	26.74676251908021	0.0
15	0.0	0.0	0.0	27.582598847801467	0.0
16	0.0	0.0	0.0	29.46846225811217	0.0
17	0.0	0.0	0.0	31.8664139051652	0.0
18	0.0	0.0	0.0	34.119109754296126	0.0
19	0.0	0.0	0.0	35.61352109902014	0.0
20	0.0	0.0	0.0	36.775567482396966	0.0
21	0.0	0.0	0.0	38.082869663695895	0.0
22	0.0	0.0	0.0	39.04549707026442	0.0
23	0.0	0.0	0.0	39.86902358560244	0.0
24	0.0	0.0	0.0	40.33187256881185	0.0
25	0.0	0.0	0.0	40.65069673543749	0.0
26	0.0	0.0	0.0	40.89935496577872	0.0
27	0.0	0.0	0.0	41.088926091880445	0.0
28	0.0	0.0	0.0	41.24279875917081	0.0
29	0.0	0.0	0.0	41.4089812398444	0.0
30	0.0	0.0	0.0	41.560391944458125	0.0
31	0.0	0.0	0.0	41.69949283568861	0.0
32	0.0	0.0	0.0	41.84474863361071	0.0
33	0.0	0.0	0.0	41.992466394209465	0.0
34	0.0	0.0	0.0	42.1180264907184	0.0
35	0.0	0.0	0.0	42.263282288640504	0.0
36	0.0	0.0	0.0	42.38761140381112	0.0
37	0.0	0.0	0.0	42.49470678024521	0.0
38	0.0	0.0	0.0	42.60795706337092	0.0
39	0.0	0.0	0.0	42.750750898616374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCTGG	30	2.1405867E-6	45.000004	2
AGCTTGG	20	6.994581E-4	45.0	2
TGTCTTG	25	3.8587594E-5	45.0	1
GGACCGA	20	6.994581E-4	45.0	8
CCTCGGG	25	3.8587594E-5	45.0	3
TACGGGA	20	6.994581E-4	45.0	4
TCGCGGG	25	3.8587594E-5	45.0	3
ATGAATG	20	6.994581E-4	45.0	21
TATTGGG	25	3.8587594E-5	45.0	3
AAGGGCT	20	6.994581E-4	45.0	5
ACCTGGG	20	6.994581E-4	45.0	3
CTTGGGT	35	1.1933844E-7	45.0	4
TCTGCGG	25	3.8587594E-5	45.0	2
GACCGAT	20	6.994581E-4	45.0	9
CGGGAAC	25	3.8587594E-5	45.0	6
GGGACCG	25	3.8587594E-5	45.0	7
CTTGCGG	25	3.8587594E-5	45.0	2
TGGGCTA	20	6.994581E-4	45.0	6
GGCTAGG	20	6.994581E-4	45.0	2
CTGCTTG	20	6.994581E-4	45.0	1
>>END_MODULE