FastQCFastQC Report
Sat 14 Jan 2017
SRR2933573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80770
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61647.631546366225084No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC5980.7403739012009409No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC3840.47542404358053736Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT3790.46923362634641574Illumina PCR Primer Index 7 (96% over 28bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC3730.46180512566546983No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC3200.3961867029837811No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG2790.3454252816639841No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC2510.3107589451529033Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC2410.29837811068466014Illumina PCR Primer Index 7 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC2100.25999752383310637Illumina PCR Primer Index 7 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.18323635012999878No Hit
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG1200.14857001361891792Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1120.1386653460443234No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG1070.1324749288102018Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG1050.12999876191655318No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT950.11761792744831001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.11514176055466138No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA850.10523709298006685No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA840.10399900953324254No Hit
TTCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT820.10152284263959391Illumina PCR Primer Index 7 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAATC302.1404376E-645.00000410
GTGTTTG253.858567E-545.0000041
CTGGGAC302.1404376E-645.0000045
GGACTAA302.1404376E-645.0000048
ACCGATG302.1404376E-645.00000417
TCTGCGG253.858567E-545.0000042
TTGGGCA302.1404376E-645.0000045
CTAATCC302.1404376E-645.00000411
CGGGACT302.1404376E-645.0000046
CTATGGG253.858567E-545.0000043
CGATGAA302.1404376E-645.00000419
CCGATGA302.1404376E-645.00000418
TGCGGGA302.1404376E-645.0000044
TATGGGA253.858567E-545.0000044
GACTAAT302.1404376E-645.0000049
TCGGGCT302.1404376E-645.0000045
TTATGGG206.9943495E-445.03
CGCGACC206.9943495E-445.010
CGCTGAA206.9943495E-445.037
CTCCTGG206.9943495E-445.02
Produced by FastQC (version 0.11.5)