##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80770
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98027733069209	33.0	31.0	34.0	30.0	34.0
2	32.23493871486938	34.0	31.0	34.0	30.0	34.0
3	32.287903924724525	33.0	31.0	34.0	30.0	34.0
4	35.897449548099544	37.0	35.0	37.0	35.0	37.0
5	35.97010028475919	37.0	35.0	37.0	35.0	37.0
6	35.95454995666708	37.0	35.0	37.0	35.0	37.0
7	35.92687879163056	37.0	35.0	37.0	35.0	37.0
8	35.75047666212703	37.0	35.0	37.0	35.0	37.0
9	37.619896000990465	39.0	37.0	39.0	35.0	39.0
10	37.29182864925096	39.0	37.0	39.0	34.0	39.0
11	37.27612975114523	39.0	37.0	39.0	34.0	39.0
12	37.252086170607896	39.0	37.0	39.0	34.0	39.0
13	37.1968428872106	39.0	37.0	39.0	34.0	39.0
14	38.3557137551071	40.0	38.0	41.0	34.0	41.0
15	38.39185341091989	40.0	38.0	41.0	34.0	41.0
16	38.515958895629566	40.0	38.0	41.0	34.0	41.0
17	38.53183112541785	40.0	38.0	41.0	34.0	41.0
18	38.138492014361766	39.0	38.0	40.0	34.0	41.0
19	37.659428005447566	38.0	37.0	40.0	34.0	41.0
20	36.91377986876316	38.0	35.0	40.0	34.0	41.0
21	36.82590070570757	37.0	35.0	40.0	33.0	41.0
22	36.8263959390863	37.0	35.0	40.0	33.0	41.0
23	36.722322644546246	37.0	35.0	40.0	33.0	41.0
24	36.64116627460691	37.0	35.0	40.0	33.0	41.0
25	36.57433453014733	37.0	35.0	40.0	33.0	41.0
26	36.52320168379349	37.0	35.0	40.0	33.0	41.0
27	36.43636251083323	37.0	35.0	40.0	33.0	41.0
28	36.36246130989229	37.0	35.0	40.0	33.0	41.0
29	36.154661384177295	37.0	35.0	40.0	32.0	41.0
30	35.84983285873468	37.0	35.0	40.0	31.0	41.0
31	35.302203788535344	37.0	35.0	40.0	29.0	41.0
32	34.66787173455491	37.0	35.0	40.0	23.0	41.0
33	33.890318187445835	37.0	34.0	40.0	18.0	41.0
34	33.2384796335273	37.0	33.0	40.0	15.0	41.0
35	32.85905658041352	37.0	33.0	40.0	10.0	41.0
36	32.47766497461929	37.0	32.0	40.0	10.0	41.0
37	32.26632413024638	36.0	31.0	40.0	10.0	41.0
38	32.11901696174322	36.0	31.0	40.0	9.0	41.0
39	32.07944781478272	36.0	31.0	40.0	8.0	41.0
40	31.888956295654328	36.0	30.0	40.0	8.0	41.0
41	31.717147455738516	36.0	30.0	40.0	8.0	41.0
42	31.55558994676241	36.0	30.0	40.0	8.0	41.0
43	31.42141884363006	36.0	29.0	40.0	8.0	41.0
44	31.265098427634022	35.0	28.0	40.0	7.0	41.0
45	31.147728116875076	35.0	27.0	40.0	7.0	41.0
46	30.8361396558128	35.0	26.0	40.0	7.0	41.0
47	30.71386653460443	35.0	25.0	39.0	7.0	41.0
48	30.674953571870745	35.0	25.0	39.0	7.0	41.0
49	30.749275721183608	35.0	26.0	40.0	7.0	41.0
50	30.59541909124675	35.0	25.0	40.0	7.0	41.0
51	29.64630432091123	35.0	23.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	2.0
14	2.0
15	6.0
16	15.0
17	19.0
18	32.0
19	70.0
20	159.0
21	197.0
22	383.0
23	644.0
24	1126.0
25	2111.0
26	3067.0
27	3403.0
28	3019.0
29	2640.0
30	2458.0
31	2363.0
32	2614.0
33	3057.0
34	4376.0
35	6528.0
36	7975.0
37	8137.0
38	14119.0
39	12243.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.547728116875078	4.5821468366967935	35.30518756964219	41.564937476785936
2	45.95889562956543	6.221369320292188	33.63748916676984	14.182245883372541
3	15.474805001857126	6.087656308035162	62.660641327225456	15.77689736288226
4	12.543023399777145	4.510337996780983	65.24204531385416	17.70459328958772
5	12.047790021047419	5.432710164665099	64.52395691469604	17.99554289959143
6	13.464157484214438	7.454500433329206	67.79621146465273	11.28513061780364
7	52.904543766249844	2.2929305435186333	40.024761668936485	4.777764021295035
8	51.59341339606289	3.986628698774297	38.43011018942677	5.98984771573604
9	47.45697660022286	4.5326234988238205	40.54228054970905	7.468119351244273
10	25.26680698279064	16.970409805620896	44.96842887210598	12.79435433948248
11	17.365358425157858	14.95976228797821	49.092484833477776	18.582394453386158
12	14.745573851677603	12.569023152160455	54.37538690107713	18.31001609508481
13	17.247740497709547	14.703479014485577	55.32994923857868	12.718831249226199
14	14.967190788659154	17.23164541290083	53.36758697536214	14.433576823077876
15	11.05856134703479	14.530147331930172	56.598984771573605	17.812306549461436
16	10.349139532004457	14.969666955552805	54.05596137179646	20.62523214064628
17	10.366472700259997	15.089761049894761	52.099789525814046	22.443976724031202
18	11.022656927076884	14.256530890181999	55.2036647270026	19.517147455738517
19	12.06264702240931	15.758326111179894	55.49956667079361	16.679460195617185
20	13.1719697907639	18.74582146836697	53.1769221245512	14.90528661631794
21	11.989600099046676	20.430853039494863	52.79435433948249	14.785192521975981
22	10.170855515661756	18.553918534109197	51.25913086542033	20.016095084808715
23	10.589327720688374	19.332673022161696	48.83372539309149	21.24427386405844
24	12.00569518385539	18.42887210597994	49.324006438033926	20.24142627213074
25	10.503899962857496	20.911229416862696	47.18955057570881	21.395320044571005
26	10.10895134332054	21.714745573851676	48.99839049151913	19.177912591308655
27	12.736164417481739	20.469233626346416	50.03838058685155	16.756221369320293
28	9.754859477528784	19.38591061037514	50.947133836820605	19.912096075275475
29	12.789402005695184	17.96706698031447	48.93277206883744	20.310758945152905
30	17.988114398910486	19.79571623127399	45.693945771945025	16.522223597870497
31	16.7921257892782	25.378234493004832	40.37885353472824	17.450786182988733
32	15.867277454500433	24.82233502538071	39.50600470471709	19.80438281540176
33	18.360777516404607	20.248854772811686	37.51392843877677	23.876439272006934
34	16.530890181998267	23.294540051999505	37.35545375758326	22.81911600841897
35	17.101646650984275	24.17729354958524	36.34517766497462	22.375882134455864
36	19.852668069827907	27.017456976600222	34.37662498452396	18.753249969047914
37	20.615327473071684	27.293549585242044	35.59985142998638	16.49127151169989
38	20.430853039494863	25.369567908877062	34.36548223350254	19.83409681812554
39	20.70818373158351	21.797697164788907	33.65853658536586	23.83558251826173
40	17.568404110437044	24.012628451157607	36.41822458833725	22.000742850068093
41	15.563947010028475	25.701374272625976	36.70174569766002	22.032933019685526
42	17.57583261111799	22.12578927819735	38.28525442614833	22.013123684536335
43	18.735916800792374	20.517518880772563	38.20725516899839	22.539309149436672
44	18.765630803516157	20.471709793240063	36.55688993438157	24.2057694688622
45	21.18484585861087	19.43791011514176	35.12690355329949	24.250340472947876
46	21.7209359910858	20.103999009533243	39.08134208245635	19.0937229169246
47	14.854525194998141	23.056828030209235	42.733688250588095	19.354958524204534
48	14.989476290701994	20.914943667203172	43.11006561842268	20.985514423672154
49	18.816392224835955	16.09260864182246	44.17110313235112	20.91989600099047
50	18.37687260121332	15.138046304320913	40.484090627708305	26.00099046675746
51	15.883372539309148	15.009285625851183	38.615822706450416	30.491519128389257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	371.0
2	569.0
3	5078.0
4	9587.0
5	5923.5
6	2260.0
7	2076.5
8	1893.0
9	1827.5
10	1762.0
11	1713.5
12	1665.0
13	1504.0
14	1343.0
15	1299.0
16	1255.0
17	1123.0
18	991.0
19	910.5
20	830.0
21	736.5
22	643.0
23	578.5
24	514.0
25	528.0
26	564.0
27	586.0
28	519.5
29	453.0
30	493.0
31	533.0
32	579.5
33	626.0
34	738.5
35	851.0
36	935.5
37	1020.0
38	1107.5
39	1195.0
40	1633.5
41	2072.0
42	2674.0
43	3276.0
44	3721.0
45	4166.0
46	5838.0
47	7510.0
48	8523.5
49	9537.0
50	9168.0
51	8799.0
52	7169.0
53	5539.0
54	4238.5
55	2938.0
56	2317.5
57	1697.0
58	1565.0
59	1433.0
60	1292.5
61	1152.0
62	1031.5
63	911.0
64	820.5
65	730.0
66	645.5
67	561.0
68	477.5
69	394.0
70	313.0
71	232.0
72	228.5
73	225.0
74	190.5
75	112.0
76	68.0
77	54.0
78	40.0
79	36.0
80	32.0
81	18.5
82	5.0
83	4.5
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	80770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.905162807973255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25229848193285	46.47022409310387
2	10.299337181954245	11.92769592670546
3	4.224930511011332	7.339358672774544
4	2.0568740645713066	4.764145103379968
5	1.024160786829164	2.9652098551442365
6	0.5730168911695531	1.9908381824935002
7	0.3207184092366902	1.2999876191655317
8	0.21808851828094933	1.0102760926086418
9	0.1689116955313235	0.8802773306920886
>10	0.7911054094505025	7.73678345920515
>50	0.03848620910840282	1.493128636870125
>100	0.027795595467179815	3.750154760430853
>500	0.002138122728244601	0.7403739012009409
>1k	0.0	0.0
>5k	0.002138122728244601	7.631546366225084
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6164	7.631546366225084	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	598	0.7403739012009409	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	384	0.47542404358053736	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	379	0.46923362634641574	Illumina PCR Primer Index 7 (96% over 28bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	373	0.46180512566546983	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	320	0.3961867029837811	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	279	0.3454252816639841	No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	251	0.3107589451529033	Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	241	0.29837811068466014	Illumina PCR Primer Index 7 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	210	0.25999752383310637	Illumina PCR Primer Index 7 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.18323635012999878	No Hit
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	120	0.14857001361891792	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	112	0.1386653460443234	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	107	0.1324749288102018	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	105	0.12999876191655318	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	95	0.11761792744831001	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.11514176055466138	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	85	0.10523709298006685	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	84	0.10399900953324254	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	82	0.10152284263959391	Illumina PCR Primer Index 7 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4939952952829021	0.0
2	0.0	0.0	0.0	1.6899839049151912	0.0
3	0.0	0.0	0.0	2.3857868020304567	0.0
4	0.0	0.0	0.0	3.3267302216169368	0.0
5	0.0	0.0	0.0	5.513185588708679	0.0
6	0.0	0.0	0.0	6.932029218769345	0.0
7	0.0	0.0	0.0	8.368206017085551	0.0
8	0.0	0.0	0.0	10.555899467624117	0.0
9	0.0	0.0	0.0	11.547604308530396	0.0
10	0.0	0.0	0.0	13.923486442986258	0.0
11	0.0	0.0	0.0	19.32029218769345	0.0
12	0.0	0.0	0.0	24.318435062523214	0.0
13	0.0	0.0	0.0	25.844991952457594	0.0
14	0.0	0.0	0.0	26.420700755230904	0.0
15	0.0	0.0	0.0	27.245264330815896	0.0
16	0.0	0.0	0.0	29.0528661631794	0.0
17	0.0	0.0	0.0	31.33713012257026	0.0
18	0.0	0.0	0.0	33.60034666336511	0.0
19	0.0	0.0	0.0	35.14671288844868	0.0
20	0.0	0.0	0.0	36.30432091122942	0.0
21	0.0	0.0	0.0	37.59564194626718	0.0
22	0.0	0.0	0.0	38.667822211217036	0.0
23	0.0	0.0	0.0	39.498576204036155	0.0
24	0.0	0.0	0.0	39.95047666212703	0.0
25	0.0	0.0	0.0	40.26618794106723	0.0
26	0.0	0.0	0.0	40.4989476290702	0.0
27	0.0	0.0	0.0	40.6772316454129	0.0
28	0.0	0.0	0.0	40.819611241797695	0.0
29	0.0	0.0	0.0	40.96818125541662	0.0
30	0.0	0.0	0.0	41.15636993933391	0.0
31	0.001238083446824316	0.0	0.0	41.29132103503776	0.0
32	0.001238083446824316	0.0	0.0	41.41636746316702	0.0
33	0.001238083446824316	0.0	0.0	41.527794973381205	0.0
34	0.001238083446824316	0.0	0.0	41.651603318063636	0.0
35	0.001238083446824316	0.0	0.0	41.78531633032066	0.0
36	0.001238083446824316	0.0	0.0	41.90169617432215	0.0
37	0.001238083446824316	0.0	0.0	42.01188560108951	0.0
38	0.001238083446824316	0.0	0.0	42.1332177788783	0.0
39	0.001238083446824316	0.0	0.0	42.27807354215674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAATC	30	2.1404376E-6	45.000004	10
GTGTTTG	25	3.858567E-5	45.000004	1
CTGGGAC	30	2.1404376E-6	45.000004	5
GGACTAA	30	2.1404376E-6	45.000004	8
ACCGATG	30	2.1404376E-6	45.000004	17
TCTGCGG	25	3.858567E-5	45.000004	2
TTGGGCA	30	2.1404376E-6	45.000004	5
CTAATCC	30	2.1404376E-6	45.000004	11
CGGGACT	30	2.1404376E-6	45.000004	6
CTATGGG	25	3.858567E-5	45.000004	3
CGATGAA	30	2.1404376E-6	45.000004	19
CCGATGA	30	2.1404376E-6	45.000004	18
TGCGGGA	30	2.1404376E-6	45.000004	4
TATGGGA	25	3.858567E-5	45.000004	4
GACTAAT	30	2.1404376E-6	45.000004	9
TCGGGCT	30	2.1404376E-6	45.000004	5
TTATGGG	20	6.9943495E-4	45.0	3
CGCGACC	20	6.9943495E-4	45.0	10
CGCTGAA	20	6.9943495E-4	45.0	37
CTCCTGG	20	6.9943495E-4	45.0	2
>>END_MODULE