##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	405987
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.55285021441573	31.0	28.0	31.0	16.0	33.0
2	28.84814292083244	31.0	30.0	33.0	16.0	34.0
3	29.045486678144865	31.0	30.0	33.0	16.0	34.0
4	32.022771665102574	35.0	30.0	37.0	19.0	37.0
5	33.63789234630666	35.0	33.0	37.0	28.0	37.0
6	32.64918581136835	35.0	33.0	37.0	17.0	37.0
7	31.846344833701572	35.0	30.0	35.0	25.0	37.0
8	32.80738545815506	35.0	32.0	35.0	27.0	37.0
9	32.68103658491528	35.0	31.0	37.0	25.0	39.0
10	31.585201003973033	35.0	28.0	39.0	15.0	39.0
11	31.969346309118272	35.0	27.0	39.0	15.0	39.0
12	32.87197373314909	35.0	31.0	39.0	22.0	39.0
13	33.03387546891896	35.0	31.0	39.0	23.0	39.0
14	33.27250872564885	36.0	31.0	39.0	18.0	41.0
15	33.76441610199341	36.0	31.0	39.0	23.0	41.0
16	34.0181927007515	36.0	32.0	40.0	25.0	41.0
17	31.981556059676787	35.0	27.0	39.0	17.0	41.0
18	32.756189237586426	36.0	30.0	39.0	21.0	39.0
19	32.54146807656403	36.0	30.0	37.0	18.0	39.0
20	32.3871133804777	34.0	30.0	37.0	24.0	39.0
21	32.84939419242488	35.0	31.0	37.0	24.0	39.0
22	33.23130543588834	35.0	31.0	38.0	25.0	39.0
23	34.2169650752364	35.0	33.0	38.0	27.0	40.0
24	32.45305637865252	35.0	31.0	37.0	22.0	39.0
25	31.557503072758486	35.0	30.0	37.0	18.0	39.0
26	31.12095953811329	33.0	27.0	37.0	20.0	39.0
27	32.57935598923118	35.0	30.0	38.0	22.0	40.0
28	32.64478172946424	35.0	31.0	38.0	22.0	39.0
29	32.970459645259574	35.0	31.0	38.0	22.0	40.0
30	31.873441760450458	35.0	30.0	38.0	18.0	40.0
31	32.10481123779825	35.0	30.0	38.0	21.0	40.0
32	32.0155793165791	35.0	30.0	38.0	19.0	40.0
33	32.11138780305773	35.0	31.0	38.0	17.0	40.0
34	31.371977427848677	35.0	30.0	38.0	14.0	40.0
35	30.9626071770771	35.0	29.0	38.0	10.0	40.0
36	30.367627534871808	35.0	27.0	38.0	9.0	40.0
37	30.519450130176583	35.0	28.0	38.0	9.0	40.0
38	30.30893353728075	35.0	26.0	38.0	8.0	40.0
39	29.933520038818976	35.0	25.0	38.0	8.0	40.0
40	29.64248362632301	34.0	24.0	38.0	8.0	40.0
41	29.18531135233394	34.0	23.0	38.0	8.0	40.0
42	29.509383305376772	34.0	24.0	38.0	8.0	40.0
43	29.576240125915362	34.0	24.0	38.0	7.0	40.0
44	29.589839083517454	34.0	24.0	38.0	7.0	40.0
45	29.883166209755483	35.0	24.0	38.0	7.0	40.0
46	29.660489128962258	35.0	24.0	38.0	7.0	40.0
47	29.651375536655113	35.0	23.0	38.0	7.0	40.0
48	29.40184291615249	34.0	23.0	38.0	7.0	40.0
49	29.26937562040164	34.0	24.0	37.0	7.0	40.0
50	29.421025796392495	34.0	24.0	38.0	7.0	40.0
51	27.677201486747112	32.0	21.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	19.0
11	37.0
12	28.0
13	59.0
14	101.0
15	229.0
16	459.0
17	714.0
18	1352.0
19	2112.0
20	3003.0
21	4308.0
22	5911.0
23	8206.0
24	11353.0
25	15169.0
26	19546.0
27	21614.0
28	22278.0
29	23030.0
30	25503.0
31	29249.0
32	33646.0
33	37385.0
34	42029.0
35	44612.0
36	32745.0
37	17685.0
38	3512.0
39	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.06259313721868	2.6555037476569447	36.372100584501474	36.909802530622905
2	37.37582730481518	11.363664353784728	37.255872724988734	14.004635616411361
3	14.323365033855765	11.361201220728741	60.10660439866301	14.208829346752482
4	12.8826785094104	3.157982891077793	66.85608159867188	17.10325700083993
5	19.640776675115212	3.554054686480109	59.46495823757903	17.340210400825644
6	15.112552864993214	11.152327537581252	61.06353159091301	12.671588006512524
7	57.292967508811856	1.2980711205038586	38.7596154556673	2.6493459150169834
8	58.38117969294583	8.186469024870254	30.262545352437392	3.169805929746519
9	54.09680605536631	3.1629091571897625	30.4844736407816	12.255811146662332
10	26.872535327485856	24.607930795813658	35.80385578848584	12.715678088214647
11	24.4101412114181	17.023944116437224	44.3083152908837	14.257599381260977
12	19.627968383224093	15.79533334811213	46.605679492200494	17.971018776463286
13	18.037030742363672	16.32466064184321	52.080239022431755	13.558069593361363
14	16.199040855988002	18.435073044210775	47.545118439753	17.82076766004823
15	13.278257678201518	15.956914876584719	53.46895343939584	17.29587400581792
16	15.857404301122942	16.37860325576927	45.721168411796434	22.042824031311348
17	16.399539886745142	16.829849231625644	49.620554352725584	17.150056528903633
18	16.35717399818221	15.346550505311745	48.524706456118054	19.771569040387995
19	16.240914117939738	18.413890099929308	45.64875229995049	19.696443482180463
20	18.278664095155754	17.863872488527957	49.505033412399904	14.35243000391638
21	18.80257249616367	20.906827065891274	45.06228031932057	15.228320118624488
22	17.34513666693761	15.174870131309623	47.96385105927037	19.516142142482394
23	16.36012975784939	21.553645806392815	45.98718678184277	16.099037653915026
24	16.78403495678433	17.33257468835209	45.75195757499625	20.131432779867335
25	15.600006896772555	22.31943387349841	43.79179628904374	18.288762940685295
26	16.18155261129051	17.958210484572167	46.7322845312781	19.12795237285923
27	17.009657944712515	18.89543261237429	44.93518265363177	19.159726789281432
28	13.977048526184335	19.48781611233857	48.27322057110203	18.261914790375062
29	17.88259229975344	18.479902065829695	44.53516984533988	19.102335789076992
30	17.360900718495913	20.545978073189534	46.03275474337848	16.060366464936067
31	19.164899368698997	19.545946052459808	42.261205408054934	19.027949170786258
32	19.325988270560387	22.01720744752911	41.55527147420977	17.10153280770074
33	18.2237362280073	19.981920603369076	40.07345062772946	21.720892540894166
34	19.829206353898034	24.162103712680455	39.17711650865668	16.83157342476483
35	19.797678250781427	23.652727796702848	37.77411591996788	18.77547803254784
36	20.439324411865403	26.761694339966553	35.10334074736383	17.695640500804213
37	20.665686339710383	22.71181096931675	36.31815797057541	20.304344720397452
38	19.25652791838162	27.733400330552456	34.92599516733294	18.084076583732976
39	22.491360560806132	22.367957594701306	34.933877193112096	20.206804651380462
40	20.349912681933166	26.420796725018285	35.823314539628115	17.40597605342043
41	23.885001243882193	23.31798801439455	34.158975533699355	18.6380352080239
42	18.57941264129147	24.70522455152505	37.68150211706286	19.03386069012062
43	21.633451317406717	22.60072366849185	35.005800678346844	20.760024335754593
44	22.309581341274473	22.07164268806637	35.793018003039506	19.825757967619655
45	19.000854707170426	22.848022227312697	35.736119629446264	22.415003436070613
46	21.53541862177853	24.216046326606516	34.396175246990666	19.852359804624285
47	17.495880409963867	23.175126297147447	40.80623271188487	18.522760581003826
48	21.024072199356137	24.221465219329684	34.54174641059936	20.212716170714824
49	18.565865409483557	22.506878299058837	38.00294098086884	20.924315310588764
50	20.13069383995054	21.474086608684516	36.502646636468654	21.89257291489629
51	20.09867311022274	21.008800774409035	35.4112323793619	23.481293736006325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	412.0
2	759.0
3	17388.5
4	34018.0
5	22883.0
6	11748.0
7	10975.0
8	10202.0
9	9961.5
10	9721.0
11	9110.0
12	8499.0
13	7862.0
14	7225.0
15	6556.5
16	5888.0
17	5360.0
18	4832.0
19	4407.0
20	3982.0
21	3785.0
22	3588.0
23	3501.0
24	3414.0
25	3479.0
26	3912.5
27	4281.0
28	4466.0
29	4651.0
30	5217.0
31	5783.0
32	6343.0
33	6903.0
34	7725.0
35	8547.0
36	8904.5
37	9262.0
38	9886.5
39	10511.0
40	11286.0
41	12061.0
42	13090.0
43	14119.0
44	15525.5
45	16932.0
46	28733.0
47	40534.0
48	34022.5
49	27511.0
50	26365.0
51	25219.0
52	22567.5
53	19916.0
54	18643.5
55	17371.0
56	16946.0
57	16521.0
58	15253.5
59	13986.0
60	12739.5
61	11493.0
62	10255.0
63	9017.0
64	7903.0
65	6789.0
66	6039.5
67	5290.0
68	4608.5
69	3927.0
70	3358.5
71	2790.0
72	2395.0
73	2000.0
74	1647.5
75	1108.0
76	921.0
77	684.5
78	448.0
79	329.0
80	210.0
81	170.5
82	131.0
83	86.5
84	42.0
85	33.5
86	25.0
87	16.5
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	405987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.115681570862684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17107511641892	34.92685939458787
2	6.821482189427234	6.018686722201661
3	3.2650076091559983	4.321141080359091
4	2.1357295545933757	3.7687665980768705
5	1.6190828653379286	3.5713472062043996
6	1.3458882937990304	3.5624867639513815
7	1.0832258387451925	3.345107231998949
8	0.8825647225846958	3.1147955413778563
9	0.7023579054066853	2.7886497933329477
>10	2.8807355191333044	18.38675494754974
>50	0.054684889559347576	1.6258983124229864
>100	0.03189951890961941	2.6640105115913566
>500	0.0028481713312160186	0.979057159999993
>1k	0.0017089027987296113	1.580664665400399
>5k	0.0011392685324864075	3.6201995500410415
>10k+	5.696342662432038E-4	5.725574520903449
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22784	5.612002354755202	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	8114	1.9985861616258649	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	6292	1.5498033188254796	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	3330	0.8202233076428556	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT	1603	0.3948402288743235	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	1357	0.3342471556971036	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	905	0.22291354156660187	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC	828	0.20394741703552086	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTA	820	0.20197691059073322	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGACAGAGTCGTATGCCG	713	0.1756213868916985	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	630	0.15517738252702673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1224177128824322	0.0
2	0.0	0.0	0.0	0.692140388731659	0.0
3	0.0	0.0	0.0	0.8256422003660215	0.0
4	0.0	0.0	0.0	1.2731934766384145	0.0
5	0.0	0.0	0.0	2.446876377815053	0.0
6	0.0	0.0	0.0	2.65624268757374	0.0
7	0.0	0.0	0.0	2.9774352380741256	0.0
8	0.0	0.0	0.0	3.405774076509839	0.0
9	0.0	0.0	0.0	3.531886488976248	0.0
10	0.0	0.0	0.0	6.192809129356359	0.0
11	0.0	0.0	0.0	6.666715929327786	0.0
12	0.0	0.0	0.0	8.840677164539752	0.0
13	0.0	0.0	0.0	9.045117208186468	0.0
14	0.0	0.0	0.0	9.169012800902491	0.0
15	0.0	0.0	0.0	9.53774381938338	0.0
16	0.0	0.0	0.0	9.785781318121023	0.0
17	0.0	0.0	0.0	10.036035636609054	0.0
18	0.0	0.0	0.0	10.261658624537239	0.0
19	2.4631330559845514E-4	0.0	0.0	10.953060073352102	0.0
20	4.926266111969103E-4	0.0	0.0	11.210457477702487	0.0
21	4.926266111969103E-4	0.0	0.0	11.455292903467353	0.0
22	4.926266111969103E-4	0.0	0.0	11.85111838556407	0.0
23	4.926266111969103E-4	0.0	0.0	12.12551140800075	0.0
24	4.926266111969103E-4	0.0	0.0	12.349656516095344	0.0
25	4.926266111969103E-4	0.0	0.0	12.535130435210979	0.0
26	4.926266111969103E-4	0.0	0.0	12.724299053910594	0.0
27	4.926266111969103E-4	0.0	0.0	12.927507531029319	0.0
28	4.926266111969103E-4	0.0	0.0	13.12037084931291	0.0
29	4.926266111969103E-4	0.0	0.0	13.31939200023646	0.0
30	4.926266111969103E-4	0.0	0.0	13.556591713527773	0.0
31	4.926266111969103E-4	0.0	0.0	13.76448014345287	0.0
32	4.926266111969103E-4	0.0	0.0	13.952663508930089	0.0
33	4.926266111969103E-4	0.0	0.0	14.157842492493602	0.0
34	4.926266111969103E-4	0.0	0.0	14.37188875505866	0.0
35	4.926266111969103E-4	0.0	0.0	14.614014734461941	0.0
36	4.926266111969103E-4	0.0	0.0	14.830524130082983	0.0
37	4.926266111969103E-4	0.0	0.0	15.044324079342442	0.0
38	4.926266111969103E-4	0.0	0.0	15.287188998662518	0.0
39	4.926266111969103E-4	0.0	0.0	15.628086613610781	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAA	25	3.8863327E-5	45.0	9
TTCGTCG	20	7.027891E-4	45.0	1
CGTTCGG	20	7.027891E-4	45.0	2
GAACGGT	25	3.8863327E-5	45.0	18
GCTACGA	20	7.027891E-4	45.0	10
CCCGACA	20	7.027891E-4	45.0	41
TCGTTCA	30	2.1620199E-6	44.999996	16
CGTTTTT	10250	0.0	44.42927	1
TACGGCT	975	0.0	43.846153	7
TGATACC	1045	0.0	43.27751	4
ACGGCTG	995	0.0	42.738693	8
AATGATC	190	0.0	42.63158	2
AATGATA	2995	0.0	42.29549	2
GAATGAT	3180	0.0	42.099056	1
ATGATAC	3040	0.0	42.039474	3
GCGATTG	145	0.0	41.89655	9
GATACCT	1080	0.0	41.875	5
ACCGGTG	70	0.0	41.785713	17
ATACGGC	1990	0.0	41.60804	6
CGACCAC	655	0.0	41.564884	12
>>END_MODULE