##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491048
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.297048353725096	31.0	28.0	31.0	25.0	34.0
2	28.420225721314413	31.0	26.0	33.0	16.0	34.0
3	28.487115312556003	31.0	26.0	33.0	16.0	34.0
4	33.01092764862091	35.0	32.0	37.0	28.0	37.0
5	34.20213706195728	35.0	33.0	37.0	30.0	37.0
6	33.4614172137958	35.0	33.0	37.0	28.0	37.0
7	33.527740668936644	35.0	32.0	37.0	29.0	37.0
8	33.30507404571447	35.0	32.0	37.0	28.0	37.0
9	34.63321711930402	37.0	34.0	39.0	28.0	39.0
10	34.727723155373816	37.0	33.0	39.0	28.0	39.0
11	34.88670761310503	37.0	34.0	39.0	27.0	39.0
12	34.99473167592578	37.0	34.0	39.0	27.0	39.0
13	35.092294439647446	37.0	34.0	39.0	28.0	39.0
14	35.85918688193415	38.0	34.0	40.0	27.0	41.0
15	36.09981101643831	38.0	34.0	40.0	28.0	41.0
16	36.10364363565272	38.0	34.0	40.0	30.0	41.0
17	35.321565305224745	37.0	33.0	40.0	27.0	41.0
18	35.101472768446264	37.0	33.0	39.0	27.0	40.0
19	34.4924956419739	37.0	33.0	38.0	27.0	40.0
20	33.8671535165605	35.0	32.0	38.0	27.0	40.0
21	34.359160407943826	35.0	33.0	38.0	27.0	40.0
22	34.91431387562927	35.0	33.0	39.0	29.0	40.0
23	35.180943614473534	35.0	34.0	39.0	30.0	40.0
24	34.70121250875678	35.0	33.0	39.0	27.0	40.0
25	33.698263713526984	35.0	33.0	38.0	24.0	40.0
26	33.78738942017888	35.0	32.0	38.0	24.0	40.0
27	34.44090190775647	35.0	33.0	39.0	27.0	40.0
28	34.164336276698	35.0	33.0	39.0	25.0	40.0
29	34.13374456264968	35.0	33.0	39.0	24.0	40.0
30	33.45580065492579	35.0	33.0	39.0	22.0	40.0
31	33.47976979847184	35.0	33.0	39.0	22.0	40.0
32	32.98917010149721	35.0	32.0	39.0	18.0	40.0
33	32.55494982160604	35.0	31.0	39.0	15.0	40.0
34	32.1316123881983	36.0	31.0	39.0	12.0	40.0
35	31.645851322070346	35.0	30.0	40.0	10.0	41.0
36	31.16143024714488	35.0	29.0	40.0	8.0	41.0
37	30.9059419853049	35.0	27.0	39.0	8.0	41.0
38	30.73709902086965	35.0	26.0	39.0	8.0	40.0
39	30.46842467538815	35.0	25.0	39.0	7.0	40.0
40	30.30868265424154	35.0	24.0	39.0	7.0	40.0
41	29.73308312018377	35.0	23.0	39.0	7.0	40.0
42	29.98147635261726	35.0	23.0	39.0	7.0	40.0
43	29.843557045339764	35.0	23.0	39.0	7.0	40.0
44	29.99220035515876	35.0	23.0	39.0	7.0	40.0
45	30.127901956631533	35.0	23.0	39.0	7.0	40.0
46	29.883591828090125	35.0	23.0	39.0	7.0	40.0
47	29.909204395496978	35.0	23.0	39.0	7.0	40.0
48	29.60415886023362	35.0	23.0	39.0	7.0	40.0
49	29.851584773789934	35.0	23.0	39.0	7.0	40.0
50	29.807686824913247	35.0	23.0	39.0	7.0	40.0
51	28.68046911910852	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	29.0
10	35.0
11	34.0
12	42.0
13	44.0
14	90.0
15	128.0
16	254.0
17	525.0
18	1051.0
19	1756.0
20	2963.0
21	4243.0
22	5927.0
23	8495.0
24	11707.0
25	17035.0
26	22226.0
27	24384.0
28	22914.0
29	21821.0
30	22190.0
31	24736.0
32	28501.0
33	33622.0
34	38699.0
35	45266.0
36	50256.0
37	53323.0
38	42358.0
39	6377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.748008341343414	2.5673661230673988	31.334004007754846	36.35062152783435
2	35.232197259738356	18.65459181179844	33.308963685831124	12.804247242632085
3	11.94669360225477	18.64563138430459	56.176992880533064	13.230682132907578
4	11.253075055799027	2.7186751600658186	70.90752838826347	15.120721395871687
5	23.456362718104952	3.5102474707156937	56.0564344015249	16.976955409654455
6	13.45448917417442	16.89936625340089	59.167739202684864	10.478405369739823
7	53.17504602401395	1.146120134895163	43.035915022563984	2.6429188185269057
8	52.11588276502501	13.015835519134585	31.543148531304478	3.325133184535931
9	48.826183998305666	3.2503950733940465	30.747706945145893	17.1757139831544
10	27.3236017660188	23.4221501767648	36.51353839135889	12.740709665857514
11	26.007029862661085	15.897427542724948	44.55715123572441	13.538391358889559
12	20.335486551212917	13.915136605790066	46.30606376566038	19.443313077336636
13	18.259518417751423	15.191386585425457	53.80838533096561	12.740709665857514
14	15.539825027288575	22.00986461608641	44.45390267346573	17.996407683159283
15	11.671975041136507	16.432405793323667	55.47624672129812	16.419372444241702
16	13.097701243055667	17.219497890226616	45.49433049314935	24.188470373568368
17	13.508048093058111	18.708761668920353	50.91762923380199	16.865561004219547
18	13.686442058617487	16.979806454766134	48.56286961763412	20.770881868982258
19	14.206350499340187	19.771386911259185	45.6433994232743	20.378863166126326
20	17.86322314722797	18.0291947019436	51.33551098874245	12.772071162085988
21	17.567121747772113	24.18113911470976	44.45166256659227	13.800076570925857
22	15.055147358303058	15.254516870041218	48.78973135009205	20.900604421563678
23	15.686450204460664	22.973314217754677	45.992041511216826	15.34819406656783
24	16.79611769114221	17.372232449780878	45.037348690963	20.79430116811391
25	13.786432283605677	26.490078363011353	42.95343836040468	16.770050992978284
26	15.081214056466985	16.790619246998258	48.66937651716329	19.458790179371466
27	17.160644173278374	19.9011094638406	44.04823968328962	18.890006679591405
28	14.062780013359182	20.04406901158339	49.55605154689562	16.337099428161807
29	20.655618188038645	15.570168293120021	45.98878317394634	17.785430344895
30	15.098116681057656	21.690751209657712	48.72456460468223	14.486567504602402
31	20.800410550496082	17.108918069109333	41.20656229126276	20.884109089131815
32	20.648286929180042	21.67140483211417	44.00751046740849	13.672797771297308
33	16.59430442645118	18.0220670891644	41.771069223375314	23.612559261009107
34	23.016487186588684	20.359720434662194	38.17203206203874	18.451760316710384
35	18.34810446229289	19.982364249523467	42.53657483586126	19.132956452322382
36	24.150388556719506	20.499217999055084	37.46578745865985	17.884605985565567
37	16.825850018735437	21.299343445039995	45.033479415454295	16.841327120770273
38	21.506247861716165	21.890528013554682	38.32497026767241	18.278253857056743
39	18.94214007591926	21.875661849758067	38.33739267851615	20.84480539580652
40	21.110767175510336	21.31339502451899	40.20645639530148	17.3693814046692
41	17.84245124712859	23.503608608527067	37.44053534481354	21.2134047995308
42	19.87198807448559	22.578240823707663	39.06115084472393	18.48862025708281
43	20.27500366562943	21.901321255763186	36.7792965249833	21.044378553624085
44	20.18458480637331	22.178483569834313	37.09576253237973	20.54116909141265
45	19.00180837718512	21.74593929717665	35.73296296899692	23.519289356641305
46	22.375816620778416	25.210570046105474	34.304996660204296	18.10861667291181
47	15.741638291979603	21.59524119841645	44.378146331926814	18.28497417767713
48	20.305957869699093	23.452900734755055	33.43950082273016	22.801640572815693
49	17.808442351867843	20.720173995210246	40.40012381681628	21.071259836105636
50	18.953136964207165	20.083372704908687	38.29564523223799	22.66784509864616
51	19.15189553770711	19.998452289796518	36.04148677929653	24.80816539319985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	690.5
2	1341.0
3	15248.0
4	29155.0
5	22309.5
6	15464.0
7	14704.0
8	13944.0
9	13547.5
10	13151.0
11	12441.0
12	11731.0
13	10898.5
14	10066.0
15	9131.5
16	8197.0
17	7621.0
18	7045.0
19	6495.5
20	5946.0
21	5460.0
22	4974.0
23	4799.0
24	4624.0
25	4589.5
26	5063.0
27	5571.0
28	5923.0
29	6275.0
30	6646.0
31	7017.0
32	8212.5
33	9408.0
34	10165.5
35	10923.0
36	11761.5
37	12600.0
38	13074.0
39	13548.0
40	14413.0
41	15278.0
42	16130.5
43	16983.0
44	18731.0
45	20479.0
46	49327.5
47	78176.0
48	55079.5
49	31983.0
50	29606.0
51	27229.0
52	23447.5
53	19666.0
54	18053.0
55	16440.0
56	15696.0
57	14952.0
58	14310.0
59	13668.0
60	13001.0
61	12334.0
62	10952.5
63	9571.0
64	7955.5
65	6340.0
66	5318.0
67	4296.0
68	3573.0
69	2850.0
70	2400.0
71	1950.0
72	1618.5
73	1287.0
74	1143.5
75	747.5
76	495.0
77	371.0
78	247.0
79	198.0
80	149.0
81	106.0
82	63.0
83	39.5
84	16.0
85	16.5
86	17.0
87	10.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.983803802959095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.11533987853163	33.632512465224295
2	5.271620678746451	4.215588938852402
3	1.8235495298604651	2.1873733988075736
4	1.0978330497796438	1.75582165083174
5	0.866408834988888	1.732116043567303
6	0.7144037441840759	1.7138747486133283
7	0.6387136128818263	1.7876739848622272
8	0.5508267352573568	1.7619318489563718
9	0.49467920811887645	1.7801240762545496
>10	4.305453718883065	29.196093738282077
>50	0.07819154839287928	2.0944779590320217
>100	0.03521208801798517	2.584555986812634
>500	0.002589124118969498	0.6544726006821067
>1k	0.0031069489427633977	3.567196595112944
>5k	0.0	0.0
>10k+	0.0020712992951755983	11.336185964108415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	16727	3.4063879702187974	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	13625	2.7746778319023804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13139	2.67570583731122	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	11261	2.2932585001873544	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT	4051	0.8249702676724069	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	3040	0.619084081393265	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	2704	0.5506589987129568	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	2686	0.5469933692836546	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	2646	0.5388475261074274	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA	2102	0.4280640589107379	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGT	852	0.17350645965363876	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTAT	689	0.14031214871051303	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTT	611	0.12442775451687003	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATG	508	0.10345220833808508	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGTTCGT	501	0.10202668578224532	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10426679265570779	0.0
2	0.0	0.0	0.0	0.8294504814193318	0.0
3	0.0	0.0	0.0	0.9298479985663316	0.0
4	0.0	0.0	0.0	1.5943451556670631	0.0
5	0.0	0.0	0.0	4.338679721737997	0.0
6	0.0	0.0	0.0	4.551489874716932	0.0
7	0.0	0.0	0.0	5.235537055440608	0.0
8	0.0	0.0	0.0	5.946465518645835	0.0
9	0.0	0.0	0.0	6.048695850507486	0.0
10	0.0	0.0	0.0	10.123042961176912	0.0
11	0.0	0.0	0.0	10.336464052394064	0.0
12	0.0	0.0	0.0	13.74529577556573	0.0
13	0.0	0.0	0.0	13.959124158941693	0.0
14	0.0	0.0	0.0	14.159308254997475	0.0
15	0.0	0.0	0.0	14.724222479268828	0.0
16	0.0	0.0	0.0	15.014621788501328	0.0
17	0.0	0.0	0.0	15.170818331405483	0.0
18	2.0364607940567928E-4	0.0	0.0	15.294838793763542	0.0
19	2.0364607940567928E-4	0.0	0.0	16.419576090321108	0.0
20	2.0364607940567928E-4	0.0	0.0	16.639310209999838	0.0
21	2.0364607940567928E-4	0.0	0.0	16.773716622407587	0.0
22	2.0364607940567928E-4	0.0	0.0	17.08081491015135	0.0
23	2.0364607940567928E-4	0.0	0.0	17.219701536306022	0.0
24	2.0364607940567928E-4	0.0	0.0	17.357162639904857	0.0
25	2.0364607940567928E-4	0.0	0.0	17.463669539434026	0.0
26	2.0364607940567928E-4	0.0	0.0	17.597668659682963	0.0
27	2.0364607940567928E-4	0.0	0.0	17.80009286261221	0.0
28	2.0364607940567928E-4	0.0	0.0	17.904359655267918	0.0
29	2.0364607940567928E-4	0.0	0.0	18.02064156660856	0.0
30	2.0364607940567928E-4	0.0	0.0	18.156066209413336	0.0
31	2.0364607940567928E-4	0.0	0.0	18.30024763363256	0.0
32	2.0364607940567928E-4	0.0	0.0	18.459091575568987	0.0
33	2.0364607940567928E-4	0.0	0.0	18.585759436959318	0.0
34	2.0364607940567928E-4	0.0	0.0	18.788183639888565	0.0
35	2.0364607940567928E-4	0.0	0.0	18.949064042619053	0.0
36	2.0364607940567928E-4	0.0	0.0	19.090394421726593	0.0
37	2.0364607940567928E-4	0.0	0.0	19.262475358824393	0.0
38	2.0364607940567928E-4	0.0	0.0	19.429668790016454	0.0
39	2.0364607940567928E-4	0.0	0.0	19.59808409768495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATC	20	7.029334E-4	45.000004	9
GCACGTG	20	7.029334E-4	45.000004	36
CGAGATA	45	3.8380676E-10	45.0	19
CGTTTTT	11370	0.0	43.773087	1
TGATACC	1820	0.0	43.516483	4
GATACCT	1840	0.0	43.165764	5
TACGGCT	1675	0.0	42.313435	7
CGGCTGT	1720	0.0	42.252907	9
ACGGCTG	1685	0.0	42.195843	8
CGATGAA	445	0.0	41.460674	19
CCGATGA	440	0.0	41.420456	18
GGCGACT	305	0.0	41.311478	10
CGACCAC	1410	0.0	41.170216	12
ATGATAC	5800	0.0	41.00431	3
GAATGAT	5925	0.0	40.97468	1
CGCTTTT	330	0.0	40.909092	1
AATGACT	385	0.0	40.90909	2
TGGGCGA	480	0.0	40.781246	6
GCGACCA	1435	0.0	40.76655	11
ATACCTG	1955	0.0	40.741684	6
>>END_MODULE