FastQCFastQC Report
Sat 14 Jan 2017
SRR2933490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739550
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363464.914610235954296No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG207772.809411128388885No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC173762.349536880535461No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC169812.2961260225812996TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT43530.5886011763910486No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA41370.5593942262186464No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC41160.5565546616185518No Hit
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT40570.548576837265905TruSeq Adapter, Index 13 (100% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT36550.4942194577783787No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC36050.4874585896829152TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT15820.21391386654046382No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.14400649043337163No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT10310.1394091001284565No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG9710.13129605841390035No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8930.12074910418497733No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT8900.12034345209924954No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8510.11506997498478805No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7810.1056047596511392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.0316525E-445.00000425
TCGTTCA750.045.016
CACCACG253.889452E-545.025
CGTTTTT245950.044.5334431
ACGGCTG22850.042.5382928
TACGGCT22800.042.434217
TGATACC24900.042.3795174
GATACCT24900.042.2891585
ACCGGTG603.6379788E-1241.25000417
CGATGAA4150.041.2048219
CACCGGT1150.041.08695616
GAATGAT79200.040.9659081
CGGCTGT23700.040.8227849
ATGATAC77350.040.8112453
ATACGGC51400.040.7101176
AATGATA76000.040.618422
GAATCTG21500.040.51
GATACGG52350.040.3581665
ATACCTG26200.040.276726
TGATACG53000.040.202834
Produced by FastQC (version 0.11.5)