##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933490.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739550
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.39391521871408	31.0	28.0	33.0	25.0	34.0
2	28.515222770603746	31.0	27.0	33.0	16.0	34.0
3	28.46528835102427	31.0	26.0	33.0	16.0	34.0
4	33.18897167196268	35.0	32.0	37.0	28.0	37.0
5	34.33920627408559	35.0	33.0	37.0	32.0	37.0
6	33.64267054289771	35.0	33.0	37.0	28.0	37.0
7	33.9453681292678	35.0	33.0	37.0	30.0	37.0
8	33.61238185383003	35.0	33.0	37.0	28.0	37.0
9	34.968909471976204	37.0	34.0	39.0	28.0	39.0
10	35.04293962544791	37.0	34.0	39.0	28.0	39.0
11	35.226965046311946	37.0	34.0	39.0	28.0	39.0
12	35.20575214657562	37.0	34.0	39.0	27.0	39.0
13	35.15551484010547	37.0	34.0	39.0	27.0	39.0
14	35.90018930430667	38.0	34.0	40.0	27.0	41.0
15	36.26535325535799	38.0	34.0	41.0	27.0	41.0
16	36.29163545399229	38.0	34.0	41.0	29.0	41.0
17	35.546160503008586	38.0	33.0	40.0	27.0	41.0
18	35.35861266986681	38.0	33.0	39.0	27.0	40.0
19	34.607934554796834	37.0	33.0	37.0	27.0	40.0
20	33.60512068149551	35.0	32.0	37.0	27.0	39.0
21	33.9326414711649	35.0	33.0	37.0	27.0	40.0
22	34.415510783584615	35.0	33.0	38.0	28.0	40.0
23	34.772875397201	35.0	33.0	38.0	30.0	40.0
24	34.2104955716314	35.0	33.0	38.0	27.0	40.0
25	33.219878304374284	35.0	32.0	38.0	23.0	40.0
26	33.14713001149347	35.0	32.0	38.0	23.0	40.0
27	34.01842336556014	35.0	33.0	38.0	26.0	40.0
28	33.621114191062134	35.0	33.0	38.0	24.0	40.0
29	33.49147454533162	35.0	33.0	38.0	23.0	40.0
30	32.78663782029613	35.0	32.0	38.0	21.0	40.0
31	32.63565005746738	35.0	32.0	38.0	21.0	40.0
32	31.876829152863227	35.0	31.0	38.0	16.0	40.0
33	30.986981272395376	35.0	29.0	38.0	11.0	40.0
34	30.34432154688662	35.0	25.0	39.0	8.0	40.0
35	29.73309985802177	35.0	22.0	39.0	7.0	40.0
36	29.094886079372593	35.0	20.0	39.0	7.0	40.0
37	28.854625109864106	35.0	18.0	39.0	7.0	40.0
38	28.547212494084242	35.0	18.0	38.0	7.0	40.0
39	28.144540599012913	34.0	18.0	38.0	7.0	40.0
40	27.848340206882565	34.0	16.0	38.0	7.0	40.0
41	27.46649584206612	33.0	15.0	38.0	7.0	40.0
42	27.5901980934352	34.0	15.0	38.0	7.0	40.0
43	27.49492123588669	34.0	15.0	38.0	7.0	40.0
44	27.514316814278953	34.0	15.0	38.0	7.0	40.0
45	27.65911973497397	34.0	15.0	39.0	7.0	40.0
46	27.445358664052463	34.0	15.0	38.0	7.0	40.0
47	27.400511121628018	34.0	15.0	38.0	7.0	40.0
48	27.262895003718477	34.0	15.0	38.0	7.0	40.0
49	27.459148130619973	34.0	15.0	38.0	7.0	40.0
50	27.437280778852006	34.0	15.0	38.0	7.0	40.0
51	26.387042120208235	32.0	12.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	30.0
10	39.0
11	33.0
12	73.0
13	91.0
14	130.0
15	230.0
16	460.0
17	845.0
18	1722.0
19	3096.0
20	5149.0
21	7781.0
22	11594.0
23	16912.0
24	24781.0
25	36133.0
26	44261.0
27	44619.0
28	40343.0
29	36595.0
30	36261.0
31	38987.0
32	44041.0
33	50007.0
34	54918.0
35	63779.0
36	64693.0
37	61593.0
38	43957.0
39	6384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.282198634304642	2.1376512744236362	26.760056791292	44.82009329997972
2	43.74254614292475	17.183422351429922	28.049624771820703	11.024406733824623
3	9.951186532350755	17.32080319112974	61.04523020755865	11.682780068960854
4	9.144885403285782	2.382394699479413	75.38651882901765	13.086201068217159
5	20.626326820363737	2.686633763775269	61.96376174700832	14.72327766885268
6	11.375025353255358	14.849165032790209	64.64079507808803	9.135014535866405
7	43.38989926306538	0.9743763099181935	53.0168345615577	2.618889865458725
8	41.98634304644717	11.355959705226152	43.031302819282	3.6263944290446894
9	39.59313095801501	2.402542086403894	42.29017645865729	15.714150496923804
10	25.647082685416805	15.841390034480426	47.3961192617132	11.11540801838956
11	22.94516935974579	12.611858562639444	52.91515110540194	11.527820972212833
12	18.53992292610371	10.891488067067812	54.59928334798189	15.969305658846597
13	17.009532824014602	12.33128253667771	61.34000405652086	9.31918058278683
14	13.217902778716786	18.133729970928268	54.88662024203907	13.761747008315867
15	9.438983165438444	13.185180177134745	64.70137245622338	12.674464201203433
16	13.092691501588805	15.019809343519707	52.313704279629505	19.57379487526198
17	13.561084443242514	15.1859914813062	57.44236359948618	13.810560475965113
18	13.399634913122846	14.164289094719763	56.93665066594551	15.499425326211885
19	12.79345547968359	16.91920762625921	53.350415793387874	16.936921100669323
20	12.710026367385572	18.067203028868907	58.97072544114664	10.252045162598877
21	13.270367115137581	20.836995470218376	55.29849232641472	10.594145088229329
22	10.73990940436752	12.678385504698802	58.914475018592384	17.66723007234129
23	11.536204448651207	19.692380501656412	56.8387532959232	11.932661753769185
24	13.771212223649517	16.4289094719762	52.61050638902035	17.18937191535393
25	11.795010479345548	23.488337502535327	50.74950983706308	13.967142181056047
26	11.363126225407342	14.617132039753905	55.19721452234467	18.822527212494084
27	13.365019268474072	16.45176120613887	53.018457169900614	17.16476235548644
28	9.148265837333513	17.267797985261307	56.33953079575418	17.244405381651003
29	14.596984652829423	18.37184774525049	50.72517071191941	16.305996890000678
30	12.358596443783382	19.571631397471435	52.44811033736732	15.621661821377867
31	14.687174633222904	15.984855655466163	49.46940707186803	19.858562639442905
32	16.8417280778852	23.220201473869245	46.51098641065513	13.427084037590426
33	14.040700425934691	19.17098235413427	43.920762625921164	22.86755459400987
34	15.266445811642216	22.708268541680752	44.29301602325739	17.732269623419647
35	12.43080251504293	22.83091068893246	42.80102765195051	21.9372591440741
36	15.81340004056521	23.360421878169156	37.45980663917247	23.366371442093165
37	14.634169427354474	25.5929957406531	40.48177946048273	19.291055371509703
38	17.649246163207355	24.29964167399094	37.01047934554797	21.040632817253734
39	17.664390507741192	23.927388276654725	36.85173416266649	21.556487052937598
40	17.41518490974241	23.138394969914135	42.158474748157666	17.28794537218579
41	16.892164153877356	22.169562571834224	35.613548779663304	25.324724494625112
42	15.718071800419173	21.202082347373402	45.00615238996687	18.073693462240552
43	17.744980055439118	21.384896220674733	37.33838144817795	23.5317422757082
44	20.02028260428639	21.13406801433304	36.848353728618754	21.997295652761814
45	15.89845176120614	20.129132580623352	37.85301872760463	26.119396930565884
46	19.820296126022583	23.361909269150157	35.90697045500642	20.910824149820836
47	13.706848759380705	20.775336353187747	45.49712663105942	20.020688256372118
48	17.157325400581435	23.821918734365493	35.594077479548375	23.426678385504697
49	15.89277263200595	19.99067000202826	42.599689000067606	21.51686836589818
50	16.450409032519776	19.636265296464067	38.959231965384355	24.954093705631802
51	15.87803393955784	20.83118112365628	37.0138597795957	26.276925157190185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	1371.0
2	2661.0
3	34216.0
4	65771.0
5	47165.5
6	28560.0
7	26695.0
8	24830.0
9	24335.5
10	23841.0
11	22888.0
12	21935.0
13	20461.0
14	18987.0
15	17615.5
16	16244.0
17	15009.0
18	13774.0
19	12922.5
20	12071.0
21	11328.5
22	10586.0
23	10423.0
24	10260.0
25	10253.5
26	10546.0
27	10845.0
28	11348.0
29	11851.0
30	12255.5
31	12660.0
32	12843.0
33	13026.0
34	13776.0
35	14526.0
36	14586.5
37	14647.0
38	14931.0
39	15215.0
40	16343.0
41	17471.0
42	20668.0
43	23865.0
44	25738.5
45	27612.0
46	67581.5
47	107551.0
48	76443.5
49	45336.0
50	40683.5
51	36031.0
52	30952.0
53	25873.0
54	25261.5
55	24650.0
56	23342.5
57	22035.0
58	19983.0
59	17931.0
60	15258.5
61	12586.0
62	10824.5
63	9063.0
64	7474.0
65	5885.0
66	4947.5
67	4010.0
68	3238.5
69	2467.0
70	2100.5
71	1734.0
72	1443.5
73	1153.0
74	973.0
75	611.0
76	429.0
77	331.0
78	233.0
79	180.5
80	128.0
81	92.0
82	56.0
83	41.0
84	26.0
85	16.5
86	7.0
87	6.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	739550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.01965397695623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.89774839348311	39.5298465846774
2	5.797374012058882	5.335862920198965
3	2.0396393172354004	2.8159048685090515
4	1.154193365088549	2.124623171354949
5	0.7954796031261461	1.8303848040795854
6	0.6133885014726859	1.6936755954730025
7	0.5054380570525197	1.628205914463941
8	0.42656279483944065	1.5704217774363542
9	0.3812135836004443	1.578898548774713
>10	2.251255395859231	17.063992185039307
>50	0.07467840667958925	2.46615751693828
>100	0.05466458871680022	4.5861728827563155
>500	0.004480703993180185	1.4061495838892568
>1k	0.002688422395908111	3.7942256980083466
>5k	0.0	0.0
>10k+	0.0011948543981813826	12.575477948400549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36346	4.914610235954296	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	20777	2.809411128388885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	17376	2.349536880535461	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	16981	2.2961260225812996	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT	4353	0.5886011763910486	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA	4137	0.5593942262186464	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	4116	0.5565546616185518	No Hit
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	4057	0.548576837265905	TruSeq Adapter, Index 13 (100% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	3655	0.4942194577783787	No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	3605	0.4874585896829152	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT	1582	0.21391386654046382	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.14400649043337163	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT	1031	0.1394091001284565	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG	971	0.13129605841390035	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	893	0.12074910418497733	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	890	0.12034345209924954	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	851	0.11506997498478805	No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	781	0.1056047596511392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13994996957609357	0.0
2	0.0	0.0	0.0	0.9302954499357717	0.0
3	0.0	0.0	0.0	1.0952606314650801	0.0
4	0.0	0.0	0.0	1.9556487052937597	0.0
5	0.0	0.0	0.0	5.124467581637482	0.0
6	0.0	0.0	0.0	5.424244472990332	0.0
7	0.0	0.0	0.0	6.18132648232033	0.0
8	0.0	0.0	0.0	7.076330200797782	0.0
9	0.0	0.0	0.0	7.232641471164897	0.0
10	0.0	0.0	0.0	10.995875870461767	0.0
11	0.0	0.0	0.0	11.462781421134475	0.0
12	0.0	0.0	0.0	14.732066797376783	0.0
13	0.0	0.0	0.0	15.040091947806099	0.0
14	0.0	0.0	0.0	15.277263200594957	0.0
15	0.0	0.0	0.0	16.07017781083091	0.0
16	0.0	0.0	0.0	16.478128591711176	0.0
17	0.0	0.0	0.0	16.73490636197688	0.0
18	0.0	0.0	0.0	16.94733283753634	0.0
19	0.0	0.0	0.0	17.88168480832939	0.0
20	0.0	0.0	0.0	18.1940369143398	0.0
21	0.0	0.0	0.0	18.45730511797715	0.0
22	0.0	0.0	0.0	18.88283415590562	0.0
23	0.0	0.0	0.0	19.140288012980868	0.0
24	0.0	0.0	0.0	19.346224055168683	0.0
25	0.0	0.0	0.0	19.53728618754648	0.0
26	0.0	0.0	0.0	19.746873098505848	0.0
27	0.0	0.0	0.0	19.99972956527618	0.0
28	0.0	0.0	0.0	20.18916908931107	0.0
29	0.0	0.0	0.0	20.435940774795483	0.0
30	0.0	0.0	0.0	20.712730714623756	0.0
31	1.3521736190926916E-4	0.0	0.0	20.904874585896827	0.0
32	1.3521736190926916E-4	0.0	0.0	21.12149279967548	0.0
33	1.3521736190926916E-4	0.0	0.0	21.353390575349874	0.0
34	1.3521736190926916E-4	0.0	0.0	21.645595294435804	0.0
35	1.3521736190926916E-4	0.0	0.0	21.95321479277939	0.0
36	1.3521736190926916E-4	0.0	0.0	22.19606517476844	0.0
37	1.3521736190926916E-4	0.0	0.0	22.461226421472517	0.0
38	1.3521736190926916E-4	0.0	0.0	22.785883307416672	0.0
39	1.3521736190926916E-4	0.0	0.0	23.426948820228517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	20	7.0316525E-4	45.000004	25
TCGTTCA	75	0.0	45.0	16
CACCACG	25	3.889452E-5	45.0	25
CGTTTTT	24595	0.0	44.533443	1
ACGGCTG	2285	0.0	42.538292	8
TACGGCT	2280	0.0	42.43421	7
TGATACC	2490	0.0	42.379517	4
GATACCT	2490	0.0	42.289158	5
ACCGGTG	60	3.6379788E-12	41.250004	17
CGATGAA	415	0.0	41.20482	19
CACCGGT	115	0.0	41.086956	16
GAATGAT	7920	0.0	40.965908	1
CGGCTGT	2370	0.0	40.822784	9
ATGATAC	7735	0.0	40.811245	3
ATACGGC	5140	0.0	40.710117	6
AATGATA	7600	0.0	40.61842	2
GAATCTG	2150	0.0	40.5	1
GATACGG	5235	0.0	40.358166	5
ATACCTG	2620	0.0	40.27672	6
TGATACG	5300	0.0	40.20283	4
>>END_MODULE