FastQCFastQC Report
Sat 14 Jan 2017
SRR2933489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485433
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444609.158833453844299No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG124962.5741966450570932No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC101742.0958608088036867No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC77491.5963068023805551TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC41820.8614989092212519TruSeq Adapter, Index 13 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT30180.6217129861381487TruSeq Adapter, Index 13 (100% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT27750.571654584669769No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA22120.45567565451874925No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC21050.43363347774049144No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC17570.3619449027981204No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT17050.35123281688719143TruSeq Adapter, Index 13 (100% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT16470.3392847210634629No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC12020.24761398586416664TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC10190.20991568352378187TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCACCAACTCTCGTATGCCG9560.1969375794393871No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT8480.1746894010089961No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC7060.14543716640607457No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT5430.1118588971083548No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT5170.1065028541528903No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.10629685250075704No Hit
GAATCTTTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC5100.10506084258795755TruSeq Adapter, Index 13 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG5010.10320682771875832No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG4990.10279482441449181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA207.0292555E-445.025
TTTCGAC207.0292555E-445.024
CCGTCAG207.0292555E-445.030
CTACGGG406.8012014E-945.03
TCGCTCG253.887464E-545.01
ACGTGAG207.0292555E-445.044
CCGGTGT253.887464E-545.018
TCGACGT207.0292555E-445.026
GCTCCGA253.887464E-545.09
CCCGCAT207.0292555E-445.042
CGTTTTT163550.044.601041
AATGATC3800.044.4078982
TACGGCT15850.043.4384847
ACGGCTG16050.042.8971988
GAATGAT48000.042.6093751
TGATACG30100.042.5332224
GATACGG30100.042.5332225
ATACGGC30000.042.456
AATGATA45150.042.159472
ACGGCGA13250.042.113218
Produced by FastQC (version 0.11.5)