##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485433
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.08897005353983	31.0	25.0	31.0	16.0	33.0
2	28.07235190026224	31.0	28.0	33.0	16.0	34.0
3	28.176467195266905	31.0	28.0	33.0	16.0	34.0
4	31.73874664474809	35.0	28.0	37.0	19.0	37.0
5	33.719689431909245	35.0	33.0	37.0	30.0	37.0
6	32.28818395123529	35.0	32.0	37.0	17.0	37.0
7	32.68704435009569	35.0	32.0	35.0	27.0	37.0
8	32.7205834790795	35.0	32.0	37.0	25.0	37.0
9	33.371451879044066	35.0	32.0	38.0	26.0	39.0
10	32.4241223814615	35.0	29.0	39.0	17.0	39.0
11	32.947943382505926	37.0	31.0	39.0	17.0	39.0
12	33.53192716605587	37.0	31.0	39.0	23.0	39.0
13	33.32241730578679	35.0	32.0	39.0	22.0	39.0
14	33.613594461027574	36.0	31.0	40.0	21.0	41.0
15	34.089833200462266	37.0	32.0	40.0	23.0	41.0
16	34.131898737827875	37.0	32.0	40.0	24.0	41.0
17	32.288787536076036	35.0	27.0	39.0	18.0	41.0
18	33.07174419538845	36.0	30.0	39.0	23.0	39.0
19	32.781374566624024	36.0	30.0	37.0	21.0	39.0
20	32.223829035108864	34.0	30.0	36.0	24.0	39.0
21	32.60850416020337	35.0	31.0	37.0	24.0	39.0
22	32.78572120148404	34.0	30.0	37.0	25.0	39.0
23	33.85931735172516	35.0	32.0	38.0	27.0	40.0
24	32.213108709131845	34.0	31.0	37.0	21.0	39.0
25	31.390542052147257	34.0	29.0	37.0	18.0	39.0
26	30.517564730869143	33.0	25.0	37.0	19.0	39.0
27	32.52412382347306	34.0	31.0	37.0	22.0	39.0
28	32.2776881670591	35.0	31.0	37.0	21.0	39.0
29	32.31985464523426	35.0	31.0	37.0	21.0	39.0
30	31.384909966977936	34.0	29.0	37.0	18.0	39.0
31	31.48885634062785	34.0	30.0	37.0	20.0	39.0
32	31.49232952848282	35.0	30.0	37.0	18.0	39.0
33	31.30358257473225	35.0	30.0	37.0	15.0	40.0
34	30.402504568086638	35.0	27.0	37.0	10.0	39.0
35	29.82446805223378	34.0	25.0	37.0	9.0	39.0
36	29.108630851219427	34.0	23.0	37.0	7.0	39.0
37	29.12214867963241	34.0	23.0	37.0	7.0	40.0
38	28.891021005988467	34.0	23.0	37.0	7.0	40.0
39	28.508218435911857	33.0	22.0	37.0	7.0	39.0
40	28.208432471628424	33.0	21.0	37.0	7.0	39.0
41	27.814561432782693	33.0	20.0	37.0	7.0	39.0
42	28.258723654963713	33.0	21.0	37.0	7.0	39.0
43	28.279319700143994	33.0	21.0	37.0	7.0	40.0
44	28.310335308889176	34.0	20.0	37.0	7.0	40.0
45	28.600387695109315	34.0	20.0	38.0	7.0	40.0
46	28.30308611075061	34.0	20.0	37.0	7.0	40.0
47	28.36478566558104	34.0	20.0	37.0	7.0	40.0
48	28.17888565466295	34.0	20.0	37.0	7.0	40.0
49	28.10726094023274	34.0	20.0	37.0	7.0	39.0
50	28.279490681515266	34.0	20.0	37.0	7.0	40.0
51	26.563066375792335	31.0	18.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	23.0
11	24.0
12	30.0
13	57.0
14	109.0
15	190.0
16	454.0
17	768.0
18	1403.0
19	2477.0
20	3984.0
21	5923.0
22	8331.0
23	11878.0
24	16294.0
25	22141.0
26	26955.0
27	28136.0
28	27799.0
29	29218.0
30	32610.0
31	36903.0
32	41756.0
33	46027.0
34	48736.0
35	48691.0
36	28972.0
37	12920.0
38	2541.0
39	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.335457210366826	2.20318766956511	29.12121755216477	42.3401375679033
2	43.45522451090058	15.77272249723443	28.610745458178577	12.16130753368642
3	10.615677137730644	15.721634087505382	61.55761969210993	12.105069082654044
4	10.523182395922817	2.3941512010926327	72.91655079073735	14.16611561224721
5	19.91871174806822	2.6926475950337125	62.04666761427426	15.341973042623803
6	12.189735761680808	13.034136533775001	63.62155024483296	11.154577459711227
7	43.22409065720707	1.0129101235391906	52.241194974383696	3.5218042448700437
8	42.21591857166695	10.268770355538251	41.31610335514891	6.199207717645896
9	38.95614842831039	2.381791101964638	42.34363959598956	16.318420873735406
10	24.695066054429756	15.610393195353428	46.739302849208855	12.955237901007965
11	21.473818220022125	13.677067690082875	52.316385577412326	12.532728512482668
12	18.45898404105201	11.234094097434662	54.206656737387036	16.100265124126295
13	17.444425904295752	13.417917611699245	59.55548963502687	9.582166848978128
14	13.375069268055531	16.654821571669004	54.021049248814975	15.94905991146049
15	9.014426295698891	14.904425533492779	62.55631570165193	13.524832469156403
16	12.661685546718084	14.679677731015403	50.3330016706734	22.325635051593114
17	12.985932147175822	16.08151897378217	55.38581019419776	15.546738684844252
18	12.636553345157827	14.067234819223252	56.21167081759996	17.084541018018964
19	12.345885013997812	17.60325317809049	51.97462883652327	18.076232971388432
20	11.551542643371999	19.20429801847011	58.359856046045486	10.884303292112403
21	13.356529119363536	21.066758955406822	53.174588460199445	12.402123465030188
22	9.979956039247435	14.44565985419203	56.64159626560205	18.932787840958483
23	11.18012166457575	20.308260872252195	54.01940123559791	14.492216227574145
24	13.92777170072904	16.91891568970383	50.11093188967376	19.042380719893373
25	11.421555600875918	22.481784303910118	48.25300298908397	17.84365710612999
26	11.047456600601937	16.456029977360416	52.392194185397365	20.10431923664028
27	12.075198843094721	16.896461509621307	51.12755004295134	19.900789604332626
28	8.398687357472607	17.509110423065593	54.25733314381181	19.834869075649987
29	13.800668681362824	19.13157943526707	48.458386636260826	18.609365247109285
30	12.200241845939605	20.2042300379249	50.40139421918164	17.194133896953854
31	13.543372617848396	17.456786003423748	47.645916120247286	21.353925258480572
32	15.008868371124336	23.948928070403124	45.91385422911092	15.12834932936162
33	12.421487620330716	18.45033197166241	43.67544027703102	25.452740130975847
34	13.849697074570539	21.97275422148886	44.55403732337933	19.623511380561272
35	10.255586249801723	22.47869427912812	42.12836786951031	25.137351601559843
36	14.172707665115475	22.277842668298202	38.29611913487546	25.253330531710866
37	12.34258898756368	24.113935393761857	41.611303722655855	21.932171896018605
38	15.584436987184638	24.307164943462848	38.308891237307726	21.799506832044795
39	15.58526099379317	23.298168851314188	38.990550704216645	22.126019450675994
40	15.710715999942321	22.00777450235151	43.648042057297296	18.63346744040887
41	14.939033811051164	19.80602884435133	38.14882795360019	27.106109390997318
42	14.635593377458886	18.763454482904955	46.62332391905783	19.977628220578328
43	16.536370621692388	19.581281041873954	39.062651282463285	24.819697053970373
44	18.12175933650988	18.844001128889055	39.46435450412312	23.569885030477945
45	15.379259341659921	17.883827428295977	39.499168783333644	27.23774444671046
46	18.544886729991575	21.417373767337615	38.73243063409368	21.305308868577125
47	11.848391024096014	19.148677572394128	47.772813137961364	21.23011826554849
48	15.410571592784175	21.59577119808501	38.16633809403152	24.827319115099304
49	15.092299040238302	17.703369981027247	45.04679327528206	22.15753770345238
50	16.088935033258966	17.489540266112936	40.01520292192743	26.406321778700665
51	14.87290728071639	17.814816874831337	38.513450877876046	28.79882496657623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	415.0
2	770.0
3	29474.0
4	58178.0
5	36757.5
6	15337.0
7	14292.0
8	13247.0
9	13161.0
10	13075.0
11	12333.0
12	11591.0
13	10859.0
14	10127.0
15	9258.0
16	8389.0
17	7838.5
18	7288.0
19	6777.5
20	6267.0
21	5831.5
22	5396.0
23	5318.5
24	5241.0
25	5195.5
26	5330.5
27	5511.0
28	5715.5
29	5920.0
30	6161.0
31	6402.0
32	6670.5
33	6939.0
34	7248.0
35	7557.0
36	7682.0
37	7807.0
38	8213.0
39	8619.0
40	9791.0
41	10963.0
42	13601.5
43	16240.0
44	18022.5
45	19805.0
46	43600.5
47	67396.0
48	56509.5
49	45623.0
50	41229.0
51	36835.0
52	29300.0
53	21765.0
54	19052.5
55	16340.0
56	14371.5
57	12403.0
58	10960.5
59	9518.0
60	8035.5
61	6553.0
62	5541.5
63	4530.0
64	3733.0
65	2936.0
66	2458.0
67	1980.0
68	1670.5
69	1361.0
70	1128.5
71	896.0
72	734.0
73	572.0
74	494.5
75	314.5
76	212.0
77	161.5
78	111.0
79	86.0
80	61.0
81	45.0
82	29.0
83	19.0
84	9.0
85	6.0
86	3.0
87	2.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.16727282809208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.33678355320265	37.64095239363161
2	7.286999956984228	6.582678303108038
3	2.829466029844329	3.83397792383392
4	1.6365427445948242	2.9567269055979617
5	1.0957376661803824	2.4745741058193107
6	0.8102653832320095	2.195848657655925
7	0.6318834677550453	1.9978317088557143
8	0.4452771532191851	1.6089563730853673
9	0.357377549210409	1.45275923410393
>10	1.4201627902705782	10.291124040829693
>50	0.08310920275044517	2.6270420934288863
>100	0.05617996387039187	5.031371749463425
>500	0.0037143777765548344	1.068893873250045
>1k	0.004642972220693543	4.534358117993421
>5k	4.642972220693543E-4	1.6250458355531876
>10k+	0.001392891666208063	14.077858683789584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44460	9.158833453844299	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	12496	2.5741966450570932	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	10174	2.0958608088036867	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	7749	1.5963068023805551	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	4182	0.8614989092212519	TruSeq Adapter, Index 13 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	3018	0.6217129861381487	TruSeq Adapter, Index 13 (100% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCAACTCT	2775	0.571654584669769	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA	2212	0.45567565451874925	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC	2105	0.43363347774049144	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1757	0.3619449027981204	No Hit
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	1705	0.35123281688719143	TruSeq Adapter, Index 13 (100% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	1647	0.3392847210634629	No Hit
TCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	1202	0.24761398586416664	TruSeq Adapter, Index 13 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	1019	0.20991568352378187	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCACCAACTCTCGTATGCCG	956	0.1969375794393871	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCAACTCTCGT	848	0.1746894010089961	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	706	0.14543716640607457	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTAT	543	0.1118588971083548	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	517	0.1065028541528903	No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.10629685250075704	No Hit
GAATCTTTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	510	0.10506084258795755	TruSeq Adapter, Index 13 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	501	0.10320682771875832	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATG	499	0.10279482441449181	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4676237503424778	0.0
2	0.0	0.0	0.0	2.2734342329425483	0.0
3	0.0	0.0	0.0	2.73055189902623	0.0
4	0.0	0.0	0.0	4.258878156202813	0.0
5	0.0	0.0	0.0	7.027540360873695	0.0
6	0.0	0.0	0.0	7.659347427966372	0.0
7	0.0	0.0	0.0	8.554218604833212	0.0
8	0.0	0.0	0.0	9.757474254943524	0.0
9	0.0	0.0	0.0	10.113651111481914	0.0
10	0.0	0.0	0.0	14.111937177736165	0.0
11	0.0	0.0	0.0	15.70247593385699	0.0
12	0.0	0.0	0.0	19.467774131548534	0.0
13	0.0	0.0	0.0	19.977834222230463	0.0
14	2.060016521332501E-4	0.0	0.0	20.25861447408808	0.0
15	2.060016521332501E-4	0.0	0.0	21.09189115696708	0.0
16	2.060016521332501E-4	0.0	0.0	21.643151578075656	0.0
17	2.060016521332501E-4	0.0	0.0	22.20574209005156	0.0
18	2.060016521332501E-4	0.0	0.0	22.74752643516201	0.0
19	2.060016521332501E-4	0.0	0.0	23.913495786236204	0.0
20	2.060016521332501E-4	0.0	0.0	24.504926529510765	0.0
21	2.060016521332501E-4	0.0	0.0	25.14353165112384	0.0
22	2.060016521332501E-4	0.0	0.0	26.081869176590796	0.0
23	2.060016521332501E-4	0.0	0.0	26.694518090035082	0.0
24	2.060016521332501E-4	0.0	0.0	27.114967462039047	0.0
25	4.120033042665002E-4	0.0	0.0	27.532532810913143	0.0
26	4.120033042665002E-4	0.0	0.0	27.90560180292646	0.0
27	4.120033042665002E-4	0.0	0.0	28.339441282319083	0.0
28	4.120033042665002E-4	0.0	0.0	28.69149810581481	0.0
29	4.120033042665002E-4	0.0	0.0	29.13913969590036	0.0
30	4.120033042665002E-4	0.0	0.0	29.65434982788562	0.0
31	6.180049563997503E-4	0.0	0.0	29.997548580339615	0.0
32	6.180049563997503E-4	0.0	0.0	30.355167448442938	0.0
33	6.180049563997503E-4	0.0	0.0	30.71937836941452	0.0
34	6.180049563997503E-4	0.0	0.0	31.16557794793514	0.0
35	6.180049563997503E-4	0.0	0.0	31.645767799057747	0.0
36	6.180049563997503E-4	0.0	0.0	32.03820094637159	0.0
37	6.180049563997503E-4	0.0	0.0	32.39849783595265	0.0
38	6.180049563997503E-4	0.0	0.0	32.84016537812633	0.0
39	6.180049563997503E-4	0.0	0.0	33.76779081768236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGA	20	7.0292555E-4	45.0	25
TTTCGAC	20	7.0292555E-4	45.0	24
CCGTCAG	20	7.0292555E-4	45.0	30
CTACGGG	40	6.8012014E-9	45.0	3
TCGCTCG	25	3.887464E-5	45.0	1
ACGTGAG	20	7.0292555E-4	45.0	44
CCGGTGT	25	3.887464E-5	45.0	18
TCGACGT	20	7.0292555E-4	45.0	26
GCTCCGA	25	3.887464E-5	45.0	9
CCCGCAT	20	7.0292555E-4	45.0	42
CGTTTTT	16355	0.0	44.60104	1
AATGATC	380	0.0	44.407898	2
TACGGCT	1585	0.0	43.438484	7
ACGGCTG	1605	0.0	42.897198	8
GAATGAT	4800	0.0	42.609375	1
TGATACG	3010	0.0	42.533222	4
GATACGG	3010	0.0	42.533222	5
ATACGGC	3000	0.0	42.45	6
AATGATA	4515	0.0	42.15947	2
ACGGCGA	1325	0.0	42.11321	8
>>END_MODULE