##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933488.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648654
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.328381540852288	31.0	28.0	31.0	25.0	34.0
2	28.474309878610168	31.0	27.0	33.0	16.0	34.0
3	28.512200649344642	31.0	26.0	33.0	16.0	34.0
4	33.0192845492358	35.0	32.0	37.0	28.0	37.0
5	34.18583250854847	35.0	33.0	37.0	30.0	37.0
6	33.47645277759792	35.0	33.0	37.0	28.0	37.0
7	33.49322134759055	35.0	32.0	37.0	29.0	37.0
8	33.37909424747246	35.0	33.0	37.0	28.0	37.0
9	34.596572286611966	37.0	34.0	39.0	28.0	39.0
10	34.59302494087757	37.0	33.0	39.0	27.0	39.0
11	34.74455256577467	37.0	34.0	39.0	27.0	39.0
12	34.78394028249267	37.0	34.0	39.0	27.0	39.0
13	34.82043431475024	37.0	34.0	39.0	27.0	39.0
14	35.57255331810179	38.0	34.0	40.0	27.0	41.0
15	35.88528707138166	38.0	34.0	40.0	27.0	41.0
16	35.88991203322573	38.0	34.0	40.0	27.0	41.0
17	35.165040221751504	37.0	33.0	40.0	27.0	41.0
18	35.019256799464735	37.0	33.0	39.0	27.0	40.0
19	34.37327450381868	37.0	33.0	38.0	27.0	40.0
20	33.67101258914614	35.0	32.0	38.0	25.0	40.0
21	34.14820998560095	35.0	33.0	38.0	27.0	40.0
22	34.74833578456311	35.0	33.0	39.0	28.0	40.0
23	35.05129545181252	35.0	33.0	39.0	29.0	40.0
24	34.59085120881086	35.0	33.0	39.0	27.0	40.0
25	33.57242227751621	35.0	32.0	38.0	24.0	40.0
26	33.64484917999427	35.0	32.0	38.0	24.0	40.0
27	34.33387599552304	35.0	33.0	39.0	27.0	40.0
28	34.00453400426113	35.0	33.0	39.0	25.0	40.0
29	34.009491963357966	35.0	33.0	39.0	24.0	40.0
30	33.340412608262646	35.0	32.0	39.0	21.0	40.0
31	33.35236196801377	35.0	33.0	39.0	22.0	40.0
32	32.81428928211342	35.0	32.0	39.0	18.0	40.0
33	32.261382801925215	35.0	31.0	39.0	15.0	40.0
34	31.942995803617954	35.0	31.0	39.0	10.0	40.0
35	31.516210491263447	35.0	30.0	40.0	10.0	41.0
36	30.887325137900945	35.0	27.0	39.0	8.0	40.0
37	30.945642823446708	35.0	27.0	40.0	8.0	40.0
38	30.48395754901689	35.0	25.0	39.0	8.0	40.0
39	30.275496643819356	35.0	24.0	39.0	7.0	40.0
40	29.957101937242353	35.0	23.0	39.0	7.0	40.0
41	29.411700228473116	35.0	22.0	39.0	7.0	40.0
42	29.81483656926497	35.0	23.0	39.0	7.0	40.0
43	29.63429347541216	35.0	23.0	39.0	7.0	40.0
44	29.846943054386468	35.0	23.0	39.0	7.0	40.0
45	29.91531849028912	35.0	23.0	39.0	7.0	40.0
46	29.770056147036787	35.0	23.0	39.0	7.0	40.0
47	29.691501170115348	35.0	23.0	39.0	7.0	40.0
48	29.56061012496647	35.0	23.0	38.0	7.0	40.0
49	29.78486835816938	35.0	23.0	39.0	7.0	40.0
50	29.679619643137944	35.0	23.0	39.0	7.0	40.0
51	28.6366383310671	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	32.0
10	33.0
11	37.0
12	69.0
13	73.0
14	123.0
15	200.0
16	324.0
17	726.0
18	1420.0
19	2490.0
20	3962.0
21	5727.0
22	8264.0
23	11512.0
24	16708.0
25	23482.0
26	29715.0
27	31799.0
28	30614.0
29	29298.0
30	30496.0
31	33630.0
32	39482.0
33	45973.0
34	51601.0
35	59696.0
36	65365.0
37	66457.0
38	51560.0
39	7771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.720858886247523	2.4432131768246244	32.25910886235189	36.576819074575965
2	35.5869230745513	17.890277405211407	33.14602237864871	13.37677714158858
3	12.53996737860246	18.03488454553583	55.93151356501309	13.493634510848619
4	11.263323744245776	2.7879269996022535	70.30342833004961	15.645320926102361
5	23.06190973924465	3.3117810111399915	56.482500686036005	17.14380856357935
6	14.00191781751134	16.135566881573226	59.1164164562309	10.746098844684532
7	53.41615098342105	1.2034150718256575	42.83100081090998	2.5494331338433125
8	52.373530418374045	12.199724352274094	32.02816910093825	3.398576128413607
9	49.152398659377724	3.1602364280494686	31.227279874941033	16.46008503763177
10	28.995427454390168	21.83968648925313	37.00832801462721	12.156558041729488
11	26.613726270091604	15.20271207762536	44.80123455648158	13.382327095801461
12	20.46391450603866	13.64009163591067	46.95461679107814	18.94137706697253
13	19.205154057479028	14.686103839643321	54.15737820162983	11.951363901247815
14	15.764336610889625	20.63904639453391	46.92917950093579	16.667437493640676
15	12.220074184387979	15.062421568355393	57.00527553981013	15.712228707446496
16	15.513201182756909	16.996118115358883	45.01937859012663	22.471302111757577
17	16.098875517610313	17.6969540001295	50.16079450677865	16.043375975481535
18	16.29358641124544	16.086079789841733	48.621915535863494	18.99841826304933
19	15.664745765847432	18.98716418922877	45.466920731237295	19.881169313686495
20	16.976693275613812	19.1175881132314	51.253364659741564	12.652353951413234
21	16.88465653491692	23.18462539350717	46.76021422823261	13.170503843343292
22	14.731890961899563	14.283732159209686	50.827714004692794	20.156662874197956
23	15.151220835761439	22.127821612138366	48.562716024259466	14.158241527840728
24	16.552892605302674	18.46315601229623	45.21979360336944	19.764157779031656
25	14.794790442978844	25.87897399846451	42.97884542452525	16.347390134031397
26	15.26206575462419	16.205095474629	47.822105467629896	20.710733303116914
27	17.181116589121473	18.486743317700963	44.86613818769328	19.46600190548428
28	13.285973724050109	19.674587684651602	48.72875215446139	18.310686436836896
29	20.644596348746788	16.70135388049715	43.754605691169715	18.899444079586345
30	15.846969262503585	21.97041874404536	45.3244719064401	16.85814008701095
31	18.78628667980156	19.37165268386536	42.34245067478194	19.49960996155115
32	21.57930113743228	21.380890274321903	39.6561186703543	17.38368991789151
33	17.072738316575556	20.43893971208071	39.43119135933795	23.057130612005786
34	22.581222038251518	20.46545615998668	41.183897732843704	15.769424068918097
35	18.78289504111591	22.068622100534338	36.716184591477116	22.432298266872632
36	21.63834648364151	24.992368812957295	36.49865721941127	16.870627483989924
37	18.047063611725203	21.79189521686446	36.87543744430775	23.285603727102586
38	20.159592016699197	28.454306918634586	32.98322372173763	18.402877342928587
39	20.52835564106596	21.25416631979453	37.43320784270197	20.784270196437546
40	22.58060537667231	24.18700879051081	34.90212039084011	18.33026544197677
41	18.657866905931357	23.72435844070953	38.81992556894739	18.79784908441172
42	20.784116031042743	21.79204938225926	38.69782657626408	18.726008010433915
43	21.112334156576544	21.38551523616597	36.47537824479615	21.026772362461344
44	21.65268386535811	21.49404767410669	35.21106784202364	21.642200618511566
45	18.633971269737025	21.76692042290651	34.949603332439175	24.649504974917292
46	22.95245230893512	23.531805862601633	33.33764996438779	20.178091864075455
47	15.88088564936006	22.132600739377235	42.58063620975127	19.405877401511436
48	19.55464700749552	24.979110589004307	33.64135579214805	21.824886611352124
49	19.209316523138682	20.777024422881844	38.84104622803529	21.17261282594419
50	19.17401264772899	20.585551002537564	36.94727851828556	23.293157831447893
51	19.085984207296956	21.17122533739097	34.394916241941004	25.347874213371075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	945.5
2	1817.0
3	21097.0
4	40377.0
5	29632.5
6	18888.0
7	17936.0
8	16984.0
9	16299.5
10	15615.0
11	14844.5
12	14074.0
13	13101.5
14	12129.0
15	11185.0
16	10241.0
17	9453.5
18	8666.0
19	8134.5
20	7603.0
21	7306.5
22	7010.0
23	6730.0
24	6450.0
25	6808.5
26	7621.0
27	8075.0
28	8741.0
29	9407.0
30	10226.0
31	11045.0
32	12118.0
33	13191.0
34	14149.5
35	15108.0
36	15633.5
37	16159.0
38	16730.0
39	17301.0
40	17772.0
41	18243.0
42	19151.5
43	20060.0
44	22470.5
45	24881.0
46	61469.0
47	98057.0
48	69207.0
49	40357.0
50	37142.5
51	33928.0
52	30609.5
53	27291.0
54	26663.0
55	26035.0
56	25718.0
57	25401.0
58	23217.0
59	21033.0
60	18479.5
61	15926.0
62	13895.5
63	11865.0
64	10450.5
65	9036.0
66	7801.5
67	6567.0
68	5497.0
69	4427.0
70	3728.5
71	3030.0
72	2610.0
73	2190.0
74	1775.5
75	1089.0
76	817.0
77	596.5
78	376.0
79	287.0
80	198.0
81	154.5
82	111.0
83	85.0
84	59.0
85	36.5
86	14.0
87	8.5
88	3.0
89	4.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.03075559406533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89027918438168	34.01050786338205
2	5.570588353571901	4.57130898501111
3	2.2019480566023915	2.710427776238396
4	1.3824491862977524	2.2689173873679027
5	1.1006000673335565	2.2579226184787506
6	0.9140451516046424	2.250237793045828
7	0.7709630874494933	2.2143238609230322
8	0.6929245843584118	2.274497541274344
9	0.594364814841575	2.1948513706328905
>10	3.7555622969865405	24.356851267738445
>50	0.07959165168466995	2.2428175286100047
>100	0.04017847797686701	2.9863935723530655
>500	0.0022959130272495435	0.6548681767669752
>1k	0.0026785651984578005	3.546430682525158
>5k	0.0	0.0
>10k+	0.0015306086848330288	11.459643575652063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20346	3.1366491226447386	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	19991	3.0819204074899713	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	16549	2.5512831185809386	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	16103	2.4825253524991755	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	4029	0.6211323756579008	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	3881	0.5983158972271813	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	3603	0.5554579174721809	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	3315	0.5110582837691588	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	3294	0.5078208104783135	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	3138	0.4837710088891767	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACATGTTCGT	1328	0.2047316442972679	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT	921	0.14198632861278895	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTAT	856	0.13196557795064856	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATG	712	0.10976576109913759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07369105871543226	0.0
2	0.0	0.0	0.0	0.6729319483114264	0.0
3	0.0	0.0	0.0	0.761422884927866	0.0
4	0.0	0.0	0.0	1.4883127214200482	0.0
5	0.0	0.0	0.0	4.384001331989011	0.0
6	0.0	0.0	0.0	4.616791078140272	0.0
7	0.0	0.0	0.0	5.232990161164504	0.0
8	0.0	0.0	0.0	5.9549467050230165	0.0
9	0.0	0.0	0.0	6.0653291277013635	0.0
10	0.0	0.0	0.0	9.760981971898731	0.0
11	0.0	0.0	0.0	9.94783043039895	0.0
12	0.0	0.0	0.0	13.162949738997987	0.0
13	0.0	0.0	0.0	13.377085472378186	0.0
14	0.0	0.0	0.0	13.571642200618511	0.0
15	0.0	0.0	0.0	14.249661606958409	0.0
16	0.0	0.0	0.0	14.571867282094923	0.0
17	0.0	0.0	0.0	14.72094521886861	0.0
18	0.0	0.0	0.0	14.831944303126166	0.0
19	0.0	0.0	0.0	15.692187206122217	0.0
20	0.0	0.0	0.0	15.871789891066731	0.0
21	0.0	0.0	0.0	16.00437213059659	0.0
22	1.5416539480215955E-4	0.0	0.0	16.274315736895172	0.0
23	1.5416539480215955E-4	0.0	0.0	16.41352708840152	0.0
24	1.5416539480215955E-4	0.0	0.0	16.533621930952403	0.0
25	1.5416539480215955E-4	0.0	0.0	16.62565867164929	0.0
26	1.5416539480215955E-4	0.0	0.0	16.76117005368039	0.0
27	1.5416539480215955E-4	0.0	0.0	16.949406000733827	0.0
28	1.5416539480215955E-4	0.0	0.0	17.054855130778506	0.0
29	1.5416539480215955E-4	0.0	0.0	17.189904016625196	0.0
30	1.5416539480215955E-4	0.0	0.0	17.349002704061025	0.0
31	1.5416539480215955E-4	0.0	0.0	17.47218085450795	0.0
32	1.5416539480215955E-4	0.0	0.0	17.622183783650453	0.0
33	1.5416539480215955E-4	0.0	0.0	17.755382684759518	0.0
34	1.5416539480215955E-4	0.0	0.0	17.96335180234763	0.0
35	1.5416539480215955E-4	0.0	0.0	18.154825222691912	0.0
36	1.5416539480215955E-4	0.0	0.0	18.31376974473294	0.0
37	1.5416539480215955E-4	0.0	0.0	18.48103919809328	0.0
38	1.5416539480215955E-4	0.0	0.0	18.693479112130657	0.0
39	1.5416539480215955E-4	0.0	0.0	19.097854942696724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGACG	20	7.031011E-4	45.0	17
TACGCCT	25	3.88892E-5	45.0	7
CGTACCT	25	3.88892E-5	45.0	19
CGTTTTT	15150	0.0	44.34654	1
GATACCT	2185	0.0	43.3524	5
TGATACC	2195	0.0	43.154896	4
CCGATGA	560	0.0	42.589287	18
TACGGCT	2065	0.0	42.384987	7
ATACCTG	2260	0.0	42.013275	6
GCCGATT	75	0.0	42.0	9
GGCACGT	70	0.0	41.785717	11
CGGCTGT	2130	0.0	41.61972	9
ACGGCTG	2115	0.0	41.595745	8
CGATGAA	590	0.0	41.5678	19
AGGCACG	65	0.0	41.53846	10
ACCGGTG	55	6.002665E-11	40.909092	17
ACACGAC	110	0.0	40.909092	26
AGACACG	110	0.0	40.909092	24
GGCGACT	440	0.0	40.909092	10
CGACCAC	1845	0.0	40.487804	12
>>END_MODULE