##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432006
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.327252399272233	31.0	27.0	31.0	16.0	33.0
2	28.509735975889225	31.0	28.0	33.0	16.0	34.0
3	28.69692319088161	31.0	30.0	33.0	16.0	34.0
4	31.83554395077846	35.0	28.0	37.0	19.0	37.0
5	33.59265612051684	35.0	33.0	37.0	28.0	37.0
6	32.45806539723986	35.0	32.0	37.0	17.0	37.0
7	32.02288162664407	35.0	31.0	35.0	25.0	37.0
8	32.681972009648014	35.0	32.0	35.0	26.0	37.0
9	32.768822655240896	35.0	31.0	37.0	25.0	39.0
10	31.671620301569885	35.0	28.0	39.0	15.0	39.0
11	32.11492201497202	35.0	27.0	39.0	16.0	39.0
12	32.943227640356845	35.0	31.0	39.0	22.0	39.0
13	32.95703994851923	35.0	31.0	39.0	22.0	39.0
14	33.19619634912478	36.0	31.0	39.0	18.0	41.0
15	33.77101243964204	36.0	31.0	40.0	22.0	41.0
16	34.00351384008555	36.0	32.0	40.0	24.0	41.0
17	32.012057702902275	34.0	27.0	39.0	18.0	41.0
18	32.825921399239824	36.0	30.0	39.0	21.0	39.0
19	32.59824169108762	36.0	30.0	37.0	19.0	39.0
20	32.16698379189178	34.0	30.0	36.0	23.0	39.0
21	32.60200321291834	35.0	31.0	37.0	23.0	39.0
22	32.927433878233174	35.0	31.0	37.0	25.0	39.0
23	33.9277301704143	35.0	32.0	38.0	27.0	40.0
24	32.253130743554486	35.0	31.0	37.0	21.0	39.0
25	31.418265024096886	34.0	29.0	37.0	18.0	39.0
26	30.779102605056412	33.0	25.0	37.0	19.0	39.0
27	32.42771165215298	34.0	30.0	37.0	22.0	39.0
28	32.320389068670345	35.0	31.0	37.0	21.0	39.0
29	32.52771952241404	35.0	31.0	38.0	21.0	40.0
30	31.517094669981436	35.0	29.0	38.0	18.0	40.0
31	31.85196733378703	35.0	30.0	37.0	20.0	40.0
32	31.690381615070162	35.0	30.0	37.0	18.0	40.0
33	31.71196001907381	35.0	30.0	38.0	16.0	40.0
34	31.030981051189105	35.0	30.0	38.0	12.0	40.0
35	30.52227515358583	35.0	28.0	38.0	10.0	40.0
36	29.869011078549836	35.0	25.0	38.0	8.0	40.0
37	30.015092382976164	35.0	25.0	38.0	8.0	40.0
38	29.70817766419911	35.0	24.0	38.0	8.0	40.0
39	29.429408387846465	34.0	23.0	38.0	8.0	40.0
40	29.1127901001375	34.0	23.0	38.0	8.0	40.0
41	28.546508613306298	33.0	22.0	37.0	7.0	40.0
42	29.180631750484945	34.0	23.0	38.0	7.0	40.0
43	29.080415549784032	34.0	23.0	38.0	7.0	40.0
44	29.181284519196492	34.0	23.0	38.0	7.0	40.0
45	29.3885547885909	34.0	23.0	38.0	7.0	40.0
46	29.263413008152664	34.0	23.0	38.0	7.0	40.0
47	29.290917255778854	34.0	23.0	38.0	7.0	40.0
48	29.028606084174758	34.0	23.0	37.0	7.0	40.0
49	28.88273542497095	34.0	23.0	37.0	7.0	40.0
50	28.99179178066971	34.0	23.0	37.0	7.0	40.0
51	27.47604431419934	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	14.0
10	18.0
11	30.0
12	27.0
13	45.0
14	114.0
15	214.0
16	511.0
17	816.0
18	1474.0
19	2418.0
20	3689.0
21	5130.0
22	7082.0
23	9645.0
24	13109.0
25	17715.0
26	22306.0
27	24407.0
28	24339.0
29	25312.0
30	27687.0
31	31588.0
32	35151.0
33	39171.0
34	43198.0
35	44779.0
36	31660.0
37	16774.0
38	3502.0
39	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.418642333671293	2.490706147599802	34.56989023300602	38.520761285722884
2	38.79737781419703	13.223658930663	34.56595510247543	13.413008152664545
3	12.927366749535887	13.141021189520515	60.50170599482415	13.429906066119452
4	11.779003069401814	2.7907019809910047	69.5749596070425	15.855335342564686
5	20.08166553242316	3.205973991101976	60.03226807035087	16.68009240612399
6	14.288921913121577	12.0074721184428	61.899371767984704	11.804234200450919
7	53.22634407855447	1.2020666379633616	42.873710087359896	2.697879196122276
8	53.951565487516376	9.141771179103994	33.05995750059027	3.8467058327893593
9	49.772919820558045	3.036068943486896	33.409952639546674	13.781058596408382
10	27.0637444850303	22.248302106915183	38.635805984176145	12.052147423878372
11	24.49433572681861	15.610894293134816	47.06601297204205	12.828757008004517
12	20.017314574334616	13.819483988648306	48.770155970055974	17.39304546696111
13	18.34997662069508	15.065300018981217	54.512900283792355	12.071823076531345
14	15.433118984458549	17.89211260954709	49.66551390489947	17.00925450109489
15	12.184552992319551	15.270852719638153	56.97467164807897	15.569922639963336
16	15.175020717304852	15.373629069966622	47.4842941996176	21.967056013110927
17	15.942602649037282	16.395837094855164	51.2951209010986	16.366439355008957
18	16.059730651889094	14.712295662560242	50.69836993004727	18.529603755503395
19	15.740059165844919	18.049286352504364	47.28638028175535	18.924274199895372
20	16.30532909265149	18.396966708795713	52.32242144785027	12.975282750702535
21	16.967125456590882	20.837905029096817	48.15673856381624	14.03823095049606
22	14.517853918695572	14.738915663208383	51.60900543048014	19.134224987615912
23	14.750026620000648	20.782813201668496	49.40394346374819	15.063216714582667
24	15.416221071003644	17.76641991083457	47.64956968190256	19.16778933625922
25	14.793081577570682	21.87469618477521	45.48918302060619	17.843039217047913
26	15.06414262764869	16.826618148822007	48.87038605945288	19.238853164076424
27	15.139836020796007	17.984703916149314	47.50489576533659	19.370564297718087
28	13.021578404003648	18.91038550390504	50.3083290509854	17.75970704110591
29	18.554834886552502	16.453012226682038	46.20074721184428	18.791405674921183
30	15.583811335953667	20.405966583797447	47.51160863506526	16.498613445183633
31	17.557163557913547	18.62358393170465	45.20816840506845	18.611084105313353
32	19.988379791021423	20.54022397837067	42.794775998481505	16.676620232126403
33	16.150470132359274	19.061772290199674	43.044078091507984	21.743679485933065
34	20.872858247339156	20.03999944445216	42.909820696934766	16.177321611273918
35	17.03217084947894	21.492062610241526	39.56148757193187	21.914278968347663
36	20.280273885084927	23.41564700490271	38.82353485831215	17.48054425170021
37	17.219205288815434	21.53187687208048	39.207557302444876	22.041360536659212
38	19.057837159669077	26.604491604283275	36.731434285634926	17.606236950412725
39	18.774507761466275	20.96915320620547	40.49087281195168	19.765466220376567
40	21.40826747776651	22.75130438003176	37.909195705615204	17.931232436586527
41	18.11247991926038	21.880714619704356	41.51146048897469	18.495344972060572
42	19.620329347277586	20.79878520205738	40.779989166817124	18.80089628384791
43	19.599959259825095	20.550177543830408	39.32607417489572	20.523789021448778
44	20.135599968518957	20.799479636856898	38.27655171455952	20.78836868006463
45	17.899751392341773	21.276787822391356	37.63211622060805	23.191344564658824
46	20.66429632921765	22.749915510432725	36.63976889209872	19.946019268250904
47	15.73380925264927	21.303870779572506	43.56860784340959	19.39371212436864
48	18.337939750836792	23.556848747471097	36.90851515951167	21.19669634218043
49	18.402985143724855	20.223561709791067	40.40198515761355	20.971467988870526
50	18.208543399860186	19.886066397225967	39.04992060295459	22.85546959995926
51	18.176830877348927	20.30735684226608	37.49392369550423	24.021888584880767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	442.0
2	793.0
3	20708.0
4	40623.0
5	26885.0
6	13147.0
7	12360.5
8	11574.0
9	11195.5
10	10817.0
11	10103.5
12	9390.0
13	8594.5
14	7799.0
15	7151.0
16	6503.0
17	5942.5
18	5382.0
19	5008.0
20	4634.0
21	4376.0
22	4118.0
23	3956.0
24	3794.0
25	3996.0
26	4392.0
27	4586.0
28	5014.5
29	5443.0
30	6026.0
31	6609.0
32	7298.0
33	7987.0
34	8884.0
35	9781.0
36	10153.0
37	10525.0
38	10745.0
39	10965.0
40	11502.0
41	12039.0
42	13024.5
43	14010.0
44	15780.5
45	17551.0
46	32573.5
47	47596.0
48	39581.5
49	31567.0
50	29211.5
51	26856.0
52	23508.5
53	20161.0
54	18764.0
55	17367.0
56	16565.0
57	15763.0
58	14394.5
59	13026.0
60	11658.5
61	10291.0
62	8966.5
63	7642.0
64	6912.0
65	6182.0
66	5319.0
67	4456.0
68	3755.0
69	3054.0
70	2560.0
71	2066.0
72	1773.5
73	1481.0
74	1194.0
75	758.5
76	610.0
77	446.0
78	282.0
79	223.0
80	164.0
81	131.5
82	99.0
83	75.5
84	52.0
85	30.5
86	9.0
87	8.0
88	7.0
89	6.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.71556295164936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68113688874134	35.27021318670411
2	6.862834323694818	6.0002533180844155
3	2.8020471395550857	3.6747920436812813
4	1.9059780694162674	3.332836171121198
5	1.4136532453268187	3.089932371894398
6	1.1406870983829798	2.9919467194497242
7	0.9356302839100639	2.8631123203017967
8	0.7737189008528562	2.7058845853691187
9	0.6126755014361057	2.410510900676708
>10	2.7650258668873278	18.062673215347964
>50	0.0622358294750015	1.8713507722771052
>100	0.03788267881087044	3.400842901578175
>500	0.0016235433776087332	0.5330155865916959
>1k	0.0032470867552174664	3.0348530602744233
>5k	0.0010823622517391555	3.969818705285689
>10k+	5.411811258695777E-4	6.787964141362157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28692	6.641574422577465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	9795	2.267329620421939	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	6985	1.6168756915413214	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	4817	1.1150308097572719	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1942	0.4495307935537932	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	1920	0.44443827169067096	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	1692	0.3916612269274038	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	1328	0.30740313791938073	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	1129	0.26133896288477476	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGCACATGTTCGTATGCCG	860	0.1990713091947797	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	833	0.19282139599912962	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACATGTTCGT	560	0.12962782924311236	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	490	0.11342435058772332	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTTTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	479	0.11087808965616218	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1409702643018847	0.0
2	0.0	0.0	0.0	0.8636454123322361	0.0
3	0.0	0.0	0.0	1.040031851409471	0.0
4	0.0	0.0	0.0	1.7490497817159947	0.0
5	0.0	0.0	0.0	3.306667037031893	0.0
6	0.0	0.0	0.0	3.6136072184182626	0.0
7	0.0	0.0	0.0	4.0274903589302005	0.0
8	0.0	0.0	0.0	4.626787590913089	0.0
9	0.0	0.0	0.0	4.800859247325269	0.0
10	0.0	0.0	0.0	7.804984190034397	0.0
11	0.0	0.0	0.0	8.373726290838553	0.0
12	0.0	0.0	0.0	10.842673481386832	0.0
13	0.0	0.0	0.0	11.103780966005102	0.0
14	0.0	0.0	0.0	11.261649143761892	0.0
15	0.0	0.0	0.0	11.761642199413897	0.0
16	0.0	0.0	0.0	12.07622116359495	0.0
17	0.0	0.0	0.0	12.359550561797754	0.0
18	0.0	0.0	0.0	12.615334046286394	0.0
19	0.0	0.0	0.0	13.406295282935885	0.0
20	0.0	0.0	0.0	13.696569029133855	0.0
21	0.0	0.0	0.0	14.00536103665227	0.0
22	0.0	0.0	0.0	14.469243482729407	0.0
23	0.0	0.0	0.0	14.770396707453138	0.0
24	0.0	0.0	0.0	15.003726800090739	0.0
25	0.0	0.0	0.0	15.227103327268603	0.0
26	0.0	0.0	0.0	15.434739332324089	0.0
27	0.0	0.0	0.0	15.680337773086485	0.0
28	0.0	0.0	0.0	15.90001064800026	0.0
29	2.314782665055578E-4	0.0	0.0	16.159960741286	0.0
30	2.314782665055578E-4	0.0	0.0	16.447919704818915	0.0
31	2.314782665055578E-4	0.0	0.0	16.666203710133654	0.0
32	2.314782665055578E-4	0.0	0.0	16.878700758785758	0.0
33	2.314782665055578E-4	0.0	0.0	17.105086503428193	0.0
34	4.629565330111156E-4	0.0	0.0	17.36573103151345	0.0
35	4.629565330111156E-4	0.0	0.0	17.66595834317116	0.0
36	4.629565330111156E-4	0.0	0.0	17.90461243593839	0.0
37	4.629565330111156E-4	0.0	0.0	18.138405485109004	0.0
38	4.629565330111156E-4	0.0	0.0	18.445577144761877	0.0
39	4.629565330111156E-4	0.0	0.0	19.01455072383254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	90	0.0	45.0	4
ACCGGTC	20	7.028393E-4	45.0	17
ACACGTT	20	7.028393E-4	45.0	38
ATGATCC	105	0.0	45.0	3
GATCCGT	25	3.8867496E-5	45.0	5
AGCTCTA	20	7.028393E-4	45.0	21
GCTAACT	20	7.028393E-4	45.0	40
TATACCG	20	7.028393E-4	45.0	27
ACGGGAA	30	2.1623437E-6	44.999996	5
CGTTTTT	11890	0.0	44.640453	1
CCGATGA	165	0.0	43.636368	18
CGATGAA	160	0.0	43.59375	19
TGATACC	1100	0.0	43.159092	4
TACGGCT	955	0.0	42.64398	7
GATACCT	1130	0.0	42.212387	5
ATGATAC	3395	0.0	41.951397	3
AATGATA	3345	0.0	41.90583	2
GAATGAT	3595	0.0	41.620308	1
ATACGGC	2245	0.0	41.29176	6
GATACGG	2305	0.0	40.8026	5
>>END_MODULE