FastQCFastQC Report
Sat 14 Jan 2017
SRR2933486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084430
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC419303.8665474027830293No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG392933.6233781802421547No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365653.37181745248656No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC280212.583938105732966No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT100460.9263852899680016No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC77830.7177042317162011No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT68210.6289940337320067No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT67300.6206025285172856No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC63590.5863910072572688TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT55810.5146482483885544No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA39490.36415444058168805No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCCCTCGTATGCCG19580.18055568363103197No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGT18290.16866003338159216No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG11610.10706085224495819No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11090.1022657064079747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC700.045.017
GCGAATC207.033111E-445.033
TACGCGG207.033111E-445.02
CTTACGG207.033111E-445.02
CTAGCGG453.8562575E-1045.02
ACGGGTA302.1653887E-644.9999965
CGCCGAT302.1653887E-644.99999616
CGTTTTT393150.044.6108361
GATACCT52150.043.0584835
CACCGGT1950.042.69230716
TGATACC52850.042.573324
ACGGCTG34950.042.4248928
CCGATGA10150.042.339918
ATACCTG53200.042.3355266
TCAAGCG800.042.187517
TACGGCT35300.042.0042467
CGCTTTT10450.041.9856451
CGGCTGT35500.041.957759
CGATGAA10300.041.94174619
GAATCTG47600.041.5966381
Produced by FastQC (version 0.11.5)