##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933486.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084430
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.28937967411451	31.0	28.0	31.0	25.0	34.0
2	28.25451711959278	31.0	25.0	33.0	16.0	34.0
3	28.138491188919524	31.0	25.0	33.0	16.0	34.0
4	33.08184760657673	35.0	32.0	37.0	28.0	37.0
5	34.27169388526691	35.0	33.0	37.0	30.0	37.0
6	33.558236124046736	35.0	33.0	37.0	28.0	37.0
7	33.97486329223648	35.0	33.0	37.0	30.0	37.0
8	33.51917689477421	35.0	33.0	37.0	28.0	37.0
9	34.99884455428197	37.0	34.0	39.0	29.0	39.0
10	35.07810831496731	37.0	34.0	39.0	28.0	39.0
11	35.559868317918166	37.0	34.0	39.0	30.0	39.0
12	35.68823437197422	37.0	35.0	39.0	30.0	39.0
13	35.74617633226672	37.0	35.0	39.0	30.0	39.0
14	36.64645758601293	38.0	35.0	40.0	31.0	41.0
15	36.77926744925906	39.0	35.0	41.0	30.0	41.0
16	36.86039486181681	38.0	35.0	41.0	31.0	41.0
17	35.992345287386	38.0	34.0	40.0	27.0	41.0
18	35.72640096640632	38.0	34.0	39.0	29.0	40.0
19	34.961210958752524	37.0	34.0	37.0	27.0	40.0
20	33.59165552410022	35.0	32.0	37.0	27.0	39.0
21	34.16738563116107	35.0	33.0	37.0	29.0	39.0
22	34.510508746530434	35.0	33.0	38.0	29.0	40.0
23	34.746311887350956	35.0	33.0	38.0	30.0	40.0
24	34.23405844545061	35.0	33.0	38.0	27.0	40.0
25	33.30596903442361	35.0	33.0	37.0	24.0	40.0
26	33.05795763673082	35.0	31.0	38.0	23.0	40.0
27	33.94957350866354	35.0	33.0	38.0	25.0	40.0
28	33.23413037263816	35.0	33.0	38.0	23.0	40.0
29	32.83571184862093	35.0	32.0	38.0	21.0	40.0
30	32.23078022555628	35.0	31.0	38.0	18.0	40.0
31	31.955366413691987	35.0	31.0	38.0	17.0	40.0
32	31.014698966277216	35.0	30.0	38.0	13.0	40.0
33	29.638239443763084	35.0	23.0	38.0	8.0	40.0
34	28.312494121335632	35.0	18.0	38.0	7.0	40.0
35	27.853827356307	34.0	16.0	38.0	7.0	40.0
36	26.998476619053328	33.0	12.0	38.0	7.0	40.0
37	27.210788155989782	34.0	12.0	38.0	7.0	40.0
38	27.01730217718064	33.0	12.0	38.0	7.0	40.0
39	26.738555738959636	33.0	10.0	38.0	7.0	40.0
40	26.572682423024077	33.0	10.0	38.0	7.0	40.0
41	26.242839095192867	33.0	10.0	38.0	7.0	40.0
42	26.395823612404673	33.0	10.0	38.0	7.0	40.0
43	26.313189417481997	33.0	10.0	38.0	7.0	40.0
44	26.460538716191916	33.0	10.0	38.0	7.0	40.0
45	26.55144453768339	33.0	10.0	39.0	7.0	40.0
46	26.307777357690217	33.0	10.0	38.0	7.0	40.0
47	26.234636629381335	33.0	10.0	38.0	7.0	40.0
48	26.03028411239084	33.0	10.0	38.0	7.0	40.0
49	26.25922835037762	33.0	10.0	38.0	7.0	40.0
50	26.225917763248894	33.0	10.0	38.0	7.0	40.0
51	25.10961150097286	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	29.0
10	45.0
11	53.0
12	86.0
13	127.0
14	186.0
15	298.0
16	589.0
17	1310.0
18	2760.0
19	5303.0
20	8992.0
21	13925.0
22	20395.0
23	30362.0
24	44834.0
25	65020.0
26	80021.0
27	74128.0
28	60116.0
29	51043.0
30	49544.0
31	52437.0
32	58084.0
33	66229.0
34	74882.0
35	84047.0
36	84561.0
37	82499.0
38	63175.0
39	9341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.63568879503518	1.869922447737521	23.30800512711747	46.18638363010983
2	45.96091956142859	20.978763036802743	22.665271156275647	10.395046245493024
3	7.892810047674816	21.18495430779303	59.898010936621084	11.024224707911069
4	7.143015224588033	1.8210488459374972	78.89250574034286	12.143430189131617
5	20.996468190662377	2.2837804192064035	62.72557933661002	13.994172053521204
6	9.39000212092989	16.069271414475807	66.60420681832852	7.93651964626578
7	38.67856846453898	0.7055319384377046	58.32179117139879	2.2941084256245214
8	36.48534253017714	12.390933485794381	47.45018120118403	3.673542782844444
9	34.051160517506894	1.8512029361046816	45.59445976227142	18.503176784117002
10	21.960200289553036	10.845236668111358	53.81011222485546	13.38445081748015
11	17.891703475558586	12.658539509235267	56.47141816438129	12.978338850824858
12	12.506016985881985	11.138201635882446	59.36298331842534	16.992798059810223
13	12.433997583984214	12.623774701917137	66.43499349888882	8.507234215209834
14	11.678485471630259	19.58761745801942	55.198768016377265	13.535129053973055
15	8.328246175410122	13.755797976817313	66.4231900629824	11.492765784790167
16	8.913715039237204	16.40161190671597	54.30908403493079	20.375589019116035
17	9.601357395129238	16.89717178609961	60.36802744298848	13.133443375782669
18	10.14588309065592	15.78893981169831	58.255212415739145	15.809964681906624
19	9.803030163311602	18.623332073070646	54.30290567394852	17.270732089669227
20	11.948857925361711	16.61720904069419	61.60711157013362	9.82682146381048
21	11.851940650848833	21.779367962892948	56.42992171002278	9.938769676235442
22	10.228046070285773	12.226791955220714	58.92173768707985	18.623424287413666
23	11.134881919533763	20.222789852733694	57.60473244008373	11.037595787648812
24	13.757918906706749	14.800033197163486	54.561659120459595	16.88038877567017
25	9.401713342493291	25.628025783130308	52.68546609739679	12.284794776979611
26	10.703595437234307	14.363767140341007	58.73666350064089	16.195973921783793
27	14.96933873094621	16.337246295288768	53.25129330616084	15.442121667604178
28	9.386774618924228	16.925020517691323	58.60175391680422	15.086450946580232
29	14.267403151886244	17.03115922650609	54.63118873509586	14.070248886511807
30	9.799341589590844	19.4788045332571	58.87636823031455	11.845485646837508
31	12.275388913991682	14.20156211097074	51.49433342862149	22.028715546416088
32	14.090720470662008	18.383943638593546	54.31415582379683	13.211180066947614
33	10.943906015141595	15.124627684590061	50.51317281890025	23.418293481368092
34	14.069603386110677	21.6206670785574	43.57164593380855	20.73808360152338
35	11.620390435528343	18.40072664902299	45.905406526931195	24.07347638851747
36	13.182778049297788	21.499958503545642	38.07502558948019	27.242237857676383
37	13.136486449102295	20.44133784568852	48.730485139658626	17.691690565550566
38	15.655690086035982	21.124277270086587	40.64688361627768	22.573149027599754
39	18.21224053189233	20.841455880047583	39.666368506957575	21.279935081102515
40	15.726510701474508	21.52808387816641	42.49642669420802	20.248978726151066
41	16.123770091199987	24.601311287957728	35.51506321293214	23.759855407910145
42	15.834862554521731	22.353494462528705	42.29272521047924	19.518917772470328
43	18.956133637025903	21.84686886198279	35.9556633438765	23.2413341571148
44	19.550086220410723	21.97274144020361	36.67299871822063	21.804173621165035
45	15.702166114917516	20.90868013610837	37.5885027157124	25.800651033261712
46	21.928939627269624	24.14457364698505	35.01682911760095	18.90965760814437
47	14.947299502964691	20.483018728733068	45.822044760842104	18.747637007460142
48	18.878858017576054	23.102920428243408	33.88701898693323	24.13120256724731
49	15.277795708344478	20.76519461837094	41.33231282793726	22.624696845347327
50	17.228221277537507	20.24104829265144	38.427284379812434	24.103446049998617
51	17.990833894303922	20.861097535110613	35.07031343655192	26.07775513403355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	1318.0
2	2567.0
3	36369.0
4	70171.0
5	54667.5
6	39164.0
7	38260.0
8	37356.0
9	37641.5
10	37927.0
11	37653.5
12	37380.0
13	36704.0
14	36028.0
15	34660.5
16	33293.0
17	31891.5
18	30490.0
19	28741.5
20	26993.0
21	25395.5
22	23798.0
23	22209.0
24	20620.0
25	19578.0
26	17765.0
27	16994.0
28	16654.0
29	16314.0
30	16405.0
31	16496.0
32	17082.5
33	17669.0
34	19001.0
35	20333.0
36	22003.0
37	23673.0
38	24825.5
39	25978.0
40	27454.5
41	28931.0
42	30661.0
43	32391.0
44	37474.0
45	42557.0
46	119759.5
47	196962.0
48	132029.0
49	67096.0
50	58061.5
51	49027.0
52	40610.0
53	32193.0
54	28554.5
55	24916.0
56	21864.0
57	18812.0
58	16457.5
59	14103.0
60	12062.5
61	10022.0
62	8283.0
63	6544.0
64	5189.5
65	3835.0
66	2984.5
67	2134.0
68	1662.0
69	1190.0
70	961.5
71	733.0
72	588.5
73	444.0
74	382.5
75	250.0
76	179.0
77	142.5
78	106.0
79	72.0
80	38.0
81	35.0
82	32.0
83	20.0
84	8.0
85	4.5
86	1.0
87	2.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1084430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.718902631609566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11517652476451	45.72293880206084
2	7.230099207585678	7.76785990698345
3	3.135307753115383	5.052758757292075
4	1.6293558976340765	3.501088432689751
5	0.915543323711228	2.459099133073183
6	0.5836868611273893	1.8813010596151223
7	0.38130440784542047	1.433827805063618
8	0.24208186138205723	1.040349755236923
9	0.1566153001043022	0.7571881851230989
>10	0.527859923338599	4.696321360291549
>50	0.039220699500487184	1.4927706820754982
>100	0.036950704737280626	3.9412518671779426
>500	0.0038345070097530304	1.4088472497118076
>1k	0.0012200704121941462	1.1362909697948096
>5k	8.714788658529615E-4	3.115437189267674
>10k+	8.714788658529615E-4	14.592668844542686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	41930	3.8665474027830293	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	39293	3.6233781802421547	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36565	3.37181745248656	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	28021	2.583938105732966	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	10046	0.9263852899680016	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	7783	0.7177042317162011	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT	6821	0.6289940337320067	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	6730	0.6206025285172856	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	6359	0.5863910072572688	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	5581	0.5146482483885544	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA	3949	0.36415444058168805	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCCCTCGTATGCCG	1958	0.18055568363103197	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGT	1829	0.16866003338159216	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG	1161	0.10706085224495819	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1109	0.1022657064079747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.221434301891317E-5	0.0	0.0	0.065841040915504	0.0
2	9.221434301891317E-5	0.0	0.0	0.7538522541796151	0.0
3	9.221434301891317E-5	0.0	0.0	0.9062825631898785	0.0
4	9.221434301891317E-5	0.0	0.0	2.0651402119085605	0.0
5	9.221434301891317E-5	0.0	0.0	6.706656953422535	0.0
6	9.221434301891317E-5	0.0	0.0	7.479597576607065	0.0
7	9.221434301891317E-5	0.0	0.0	8.361812196269007	0.0
8	9.221434301891317E-5	0.0	0.0	9.357542672187233	0.0
9	9.221434301891317E-5	0.0	0.0	9.558662154311481	0.0
10	9.221434301891317E-5	0.0	0.0	14.118569202253719	0.0
11	9.221434301891317E-5	0.0	0.0	14.341543483673451	0.0
12	9.221434301891317E-5	0.0	0.0	17.50799959425689	0.0
13	9.221434301891317E-5	0.0	0.0	17.776619975470986	0.0
14	9.221434301891317E-5	0.0	0.0	17.949153011259373	0.0
15	9.221434301891317E-5	0.0	0.0	18.45070682293924	0.0
16	9.221434301891317E-5	0.0	0.0	18.76552659000581	0.0
17	9.221434301891317E-5	0.0	0.0	18.953274992392316	0.0
18	9.221434301891317E-5	0.0	0.0	19.09473179458333	0.0
19	9.221434301891317E-5	0.0	0.0	20.028217588963788	0.0
20	9.221434301891317E-5	0.0	0.0	20.221775494960486	0.0
21	9.221434301891317E-5	0.0	0.0	20.338979924937526	0.0
22	1.8442868603782634E-4	0.0	0.0	20.60815359220973	0.0
23	1.8442868603782634E-4	0.0	0.0	20.7347638851747	0.0
24	1.8442868603782634E-4	0.0	0.0	20.855288031500418	0.0
25	1.8442868603782634E-4	0.0	0.0	20.9468568741182	0.0
26	1.8442868603782634E-4	0.0	0.0	21.0809365288677	0.0
27	1.8442868603782634E-4	0.0	0.0	21.294412732956484	0.0
28	1.8442868603782634E-4	0.0	0.0	21.407283088811635	0.0
29	1.8442868603782634E-4	0.0	0.0	21.522643231928296	0.0
30	1.8442868603782634E-4	0.0	0.0	21.65690731536383	0.0
31	1.8442868603782634E-4	0.0	0.0	21.784993037817102	0.0
32	1.8442868603782634E-4	0.0	0.0	21.92949291332774	0.0
33	1.8442868603782634E-4	0.0	0.0	22.063664782420258	0.0
34	1.8442868603782634E-4	0.0	0.0	22.242007321818836	0.0
35	1.8442868603782634E-4	0.0	0.0	22.37221397416154	0.0
36	1.8442868603782634E-4	0.0	0.0	22.500115267928773	0.0
37	1.8442868603782634E-4	0.0	0.0	22.65143900482281	0.0
38	1.8442868603782634E-4	0.0	0.0	22.79732209547873	0.0
39	1.8442868603782634E-4	0.0	0.0	22.952242191750504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	70	0.0	45.0	17
GCGAATC	20	7.033111E-4	45.0	33
TACGCGG	20	7.033111E-4	45.0	2
CTTACGG	20	7.033111E-4	45.0	2
CTAGCGG	45	3.8562575E-10	45.0	2
ACGGGTA	30	2.1653887E-6	44.999996	5
CGCCGAT	30	2.1653887E-6	44.999996	16
CGTTTTT	39315	0.0	44.610836	1
GATACCT	5215	0.0	43.058483	5
CACCGGT	195	0.0	42.692307	16
TGATACC	5285	0.0	42.57332	4
ACGGCTG	3495	0.0	42.424892	8
CCGATGA	1015	0.0	42.3399	18
ATACCTG	5320	0.0	42.335526	6
TCAAGCG	80	0.0	42.1875	17
TACGGCT	3530	0.0	42.004246	7
CGCTTTT	1045	0.0	41.985645	1
CGGCTGT	3550	0.0	41.95775	9
CGATGAA	1030	0.0	41.941746	19
GAATCTG	4760	0.0	41.596638	1
>>END_MODULE