SRR2933485.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933485.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611751
Sequences flagged as poor quality	0
Sequence length	51
%GC	33

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32905	5.37882242938712	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	18855	3.082136359401129	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	13515	2.2092321876057417	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	12023	1.9653421081453075	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	4210	0.6881884949922435	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	3237	0.529136854700687	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT	3145	0.5140980562352984	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	2706	0.44233683312328054	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	1949	0.3185936761852453	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	1865	0.3048625993255426	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCATCTTCCCTCGTATGCCG	1542	0.2520633394959714	No Hit
GAATCTTTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	1171	0.19141775003228437	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA	1095	0.17899439477826765	No Hit
GAATGCTGCCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	1021	0.16689796992567238	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	925	0.1512053106574407	No Hit
GAATCTGTCTCTTATCCACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	900	0.147118680639672	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	855	0.13976274660768842	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC	755	0.12341622653661374	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	722	0.11802187491315912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	694	0.11344484929325821	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCATCTTCCCTCGTATGCCGTCTTC	689	0.11262752328970446	No Hit
GAATGACTCTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	657	0.10739663686696056	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	642	0.10494465885629937	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	636	0.10396386765203489	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGAT	55	1.8189894E-12	45.000004	5
GCGACCG	55	1.8189894E-12	45.000004	11
CGTGCGG	25	3.888658E-5	45.000004	2
TCGTGCG	25	3.888658E-5	45.000004	1
CGGTAGT	25	3.888658E-5	45.000004	12
ACCGCCG	20	7.030696E-4	45.0	14
CGACCAA	35	1.210683E-7	45.0	29
TGTTCCG	20	7.030696E-4	45.0	1
CCCGATG	20	7.030696E-4	45.0	17
GCGGGTC	30	2.1638316E-6	44.999996	5
CGCCGGT	30	2.1638316E-6	44.999996	28
CGTTTTT	23915	0.0	44.576626	1
CGGCTGC	140	0.0	43.392857	9
ACGGCTG	1825	0.0	42.90411	8
GATACCT	2580	0.0	42.383724	5
TACGGCT	1850	0.0	42.324326	7
TGATACC	2605	0.0	41.890594	4
CCGATGA	215	0.0	41.860466	18
GATCCGT	70	0.0	41.785713	5
CGGCTGT	1800	0.0	41.750004	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content