##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703970
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.42458769549839	31.0	28.0	31.0	25.0	34.0
2	28.687756580536103	31.0	28.0	33.0	16.0	34.0
3	28.703404974643806	31.0	28.0	34.0	16.0	34.0
4	33.066639203375146	35.0	32.0	37.0	28.0	37.0
5	34.169977413810244	35.0	33.0	37.0	30.0	37.0
6	33.60908277341364	35.0	33.0	37.0	28.0	37.0
7	33.41039817037658	35.0	32.0	37.0	28.0	37.0
8	33.46779834367942	35.0	33.0	37.0	28.0	37.0
9	34.51994261119082	37.0	34.0	39.0	28.0	39.0
10	34.47439095415998	37.0	32.0	39.0	27.0	39.0
11	34.49350540505988	37.0	33.0	39.0	27.0	39.0
12	34.626469877977755	37.0	33.0	39.0	27.0	39.0
13	34.68188701223063	37.0	33.0	39.0	27.0	39.0
14	35.492212736338196	38.0	34.0	40.0	27.0	41.0
15	35.824499623563504	38.0	34.0	40.0	27.0	41.0
16	35.78273790076282	38.0	34.0	40.0	27.0	41.0
17	35.08832336605253	37.0	33.0	40.0	27.0	41.0
18	34.93072716166882	37.0	33.0	39.0	27.0	40.0
19	34.280841513132664	37.0	32.0	38.0	27.0	40.0
20	33.80090770913533	35.0	32.0	38.0	27.0	40.0
21	34.15887750898476	35.0	33.0	38.0	27.0	40.0
22	34.77740102561189	35.0	33.0	39.0	29.0	40.0
23	35.149639899427534	35.0	33.0	39.0	30.0	40.0
24	34.65225932923278	35.0	33.0	39.0	27.0	40.0
25	33.62701677628308	35.0	32.0	38.0	24.0	40.0
26	33.73490063497024	35.0	32.0	38.0	24.0	40.0
27	34.381862863474296	35.0	33.0	39.0	27.0	40.0
28	34.16160631845107	35.0	33.0	39.0	25.0	40.0
29	34.23271304174894	35.0	33.0	39.0	25.0	40.0
30	33.535250081679614	35.0	33.0	39.0	22.0	40.0
31	33.48447234967399	35.0	33.0	39.0	23.0	40.0
32	32.910065769848146	35.0	32.0	39.0	19.0	40.0
33	32.50749463755558	35.0	31.0	39.0	15.0	40.0
34	32.07261531031152	35.0	31.0	39.0	12.0	40.0
35	31.64725485461028	35.0	30.0	40.0	10.0	41.0
36	31.11446510504709	35.0	29.0	39.0	8.0	41.0
37	31.17874625339148	35.0	29.0	39.0	8.0	40.0
38	30.776308649516313	35.0	26.0	39.0	8.0	40.0
39	30.53601005724676	35.0	26.0	39.0	7.0	40.0
40	30.149905535747262	35.0	24.0	39.0	8.0	40.0
41	29.826158785175505	35.0	23.0	39.0	7.0	40.0
42	30.000474452036308	35.0	23.0	39.0	7.0	40.0
43	29.979036038467548	35.0	23.0	39.0	7.0	40.0
44	30.18193815077347	35.0	24.0	39.0	7.0	40.0
45	30.22306490333395	35.0	24.0	39.0	7.0	40.0
46	30.049591601914855	35.0	23.0	39.0	7.0	40.0
47	30.03115331619245	35.0	23.0	39.0	7.0	40.0
48	29.843875449237892	35.0	23.0	38.0	7.0	40.0
49	30.01755472534341	35.0	24.0	39.0	7.0	40.0
50	30.03448726508232	35.0	24.0	39.0	7.0	40.0
51	28.894104862423113	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	36.0
10	47.0
11	43.0
12	63.0
13	96.0
14	106.0
15	222.0
16	388.0
17	676.0
18	1388.0
19	2473.0
20	3921.0
21	5827.0
22	8333.0
23	11913.0
24	17138.0
25	24544.0
26	30950.0
27	33426.0
28	32283.0
29	31788.0
30	33708.0
31	37906.0
32	43804.0
33	50577.0
34	56350.0
35	64409.0
36	70476.0
37	73865.0
38	58316.0
39	8880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.198886316178246	2.537608136710371	34.63258377487677	35.630921772234615
2	34.45771836868048	15.980794636135062	34.870093896046704	14.691393099137748
3	13.214341520235237	16.239612483486514	55.92766737218916	14.618378624089095
4	11.994971376621162	2.9525405912183755	67.81695242695001	17.23553560521045
5	21.759307925053626	3.515632768441837	56.79432362174525	17.930735684759295
6	14.811852777817236	14.16708098356464	59.279230649033344	11.741835589584783
7	56.40140915095814	1.2335752944017502	39.69558361862011	2.669431936020001
8	55.60322172819865	10.359674417943946	30.797619216727984	3.239484637129423
9	52.27808003181954	3.3265622114578743	29.89630239924997	14.49905535747262
10	29.22922851826072	23.3306817051863	35.73987527877608	11.700214497776894
11	27.583135644984875	15.850249300396325	43.03990226856258	13.526712786056224
12	21.62506925011009	14.768385016406949	45.42949273406538	18.17705299941759
13	20.203843913803148	15.02010028836456	51.79482080202281	12.98123499580948
14	16.535931928917424	20.7785843146725	45.51032004204725	17.175163714362828
15	13.51719533502848	15.540719064732873	54.19918462434479	16.74290097589386
16	17.32786908533034	18.00133528417404	43.58268108015967	21.088114550335952
17	17.30073724732588	18.097504155006604	48.30134806880975	16.300410528857764
18	17.66254243788798	16.684517806156514	47.21294941545804	18.439990340497463
19	16.69147833004247	19.556515192408767	43.69703254400045	20.054973933548304
20	18.731764137676322	19.715896984246488	48.201912013296024	13.35042686478117
21	18.287711124053583	23.333238632328083	44.942256062048095	13.436794181570239
22	16.21915706635226	15.284031989999574	48.77892523829141	19.717885705356764
23	16.56462633350853	21.868403483102973	47.08751793400287	14.479452249385627
24	18.499651973805705	18.08542977683708	44.15486455388724	19.260053695469978
25	16.047842947852892	25.177209256076257	41.80263363495603	16.97231416111482
26	15.876386777845648	15.921417105842577	46.512493430117765	21.689702686194014
27	17.937838260153132	18.23046436637925	43.57671491682884	20.254982456638775
28	13.119735215989317	20.34760003977442	46.69872295694419	19.833941787292073
29	19.04186257937128	17.393354830461526	42.366294018211	21.198488571956194
30	16.63778286006506	21.001889285054762	44.663693055101774	17.696634799778398
31	20.814665397673195	19.213460800886402	40.528289557793656	19.443584243646747
32	24.129721436993055	20.796766907680723	39.37241643820049	15.70109521712573
33	19.301816838785744	21.154168501498642	37.53327556572013	22.010739093995483
34	22.30606417887126	20.45058738298507	38.89171413554555	18.35163430259812
35	17.243064335127915	22.550108669403528	39.380655425657345	20.826171569811216
36	18.41612568717417	26.082361464266945	36.0025285168402	19.49898433171868
37	21.534724491100473	22.682074520221033	36.07525888887311	19.70794209980539
38	19.926275267411963	28.734605167833855	32.658351918405614	18.680767646348567
39	26.440189212608487	21.897666093725583	32.89912922425672	18.763015469409208
40	20.526158785175504	23.49574555733909	37.425884625765306	18.552211031720102
41	21.229029646149694	23.957697060954303	34.56056366038325	20.25270963251275
42	19.014020483827434	22.056621731039673	39.84828898958763	19.081068795545264
43	21.05501654900067	20.994644658153046	35.427361961447225	22.522976831399067
44	22.667443214909728	21.05501654900067	33.664076594173046	22.61346364191656
45	19.479523275139567	21.39508785885762	34.40501725925821	24.720371606744607
46	23.411509013168175	23.666491469806953	32.533914797505574	20.3880847195193
47	16.683949600125008	22.594854894384703	40.7541514553177	19.967044050172593
48	20.580422461184426	24.139380939528674	33.05410741935025	22.226089179936643
49	19.154509425117546	20.682415443839936	39.072119550549026	21.090955580493485
50	19.215875676520305	20.68838160717076	36.77074307143771	23.32499964487123
51	18.91799366450275	20.571757319203943	34.439677827180134	26.070571189113174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	1041.0
2	2002.0
3	22963.0
4	43924.0
5	31735.5
6	19547.0
7	18241.0
8	16935.0
9	16030.0
10	15125.0
11	14387.5
12	13650.0
13	12728.0
14	11806.0
15	11003.0
16	10200.0
17	9436.5
18	8673.0
19	8110.5
20	7548.0
21	7264.5
22	6981.0
23	6819.5
24	6658.0
25	6890.5
26	7743.5
27	8364.0
28	8817.5
29	9271.0
30	10381.5
31	11492.0
32	12650.5
33	13809.0
34	14737.5
35	15666.0
36	16304.5
37	16943.0
38	17785.0
39	18627.0
40	19548.0
41	20469.0
42	21267.0
43	22065.0
44	24631.5
45	27198.0
46	61849.0
47	96500.0
48	69135.0
49	41770.0
50	39279.0
51	36788.0
52	34426.5
53	32065.0
54	31977.5
55	31890.0
56	30762.0
57	29634.0
58	28679.5
59	27725.0
60	24606.0
61	21487.0
62	19307.0
63	17127.0
64	14714.0
65	12301.0
66	10206.5
67	8112.0
68	6588.5
69	5065.0
70	4258.0
71	3451.0
72	2896.0
73	2341.0
74	1969.5
75	1282.5
76	967.0
77	715.5
78	464.0
79	378.0
80	292.0
81	218.5
82	145.0
83	101.5
84	58.0
85	38.0
86	18.0
87	14.5
88	11.0
89	7.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.28992890094977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28507643502789	34.32245321778725
2	6.397677880867428	5.539100411878599
3	3.2339768584738735	4.199958848119527
4	2.325686805630975	4.027152658465676
5	1.7646909116878828	3.8196672049560343
6	1.4958128028954643	3.8852177931885024
7	1.1692460964192812	3.5431606267192626
8	0.9449293425690811	3.2724739244989394
9	0.746660442046425	2.909058970440732
>10	2.5212077762772904	15.579288175433033
>50	0.061351814519232016	1.8846897003708087
>100	0.048014459852553336	3.6941763586757665
>500	0.0016671687448802815	0.43230798242620083
>1k	0.0026674699918084505	3.0720570250340007
>5k	0.0	0.0
>10k+	0.0013337349959042252	9.819237102005731
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18980	2.696137619500831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	17999	2.556785090273733	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	16712	2.373964799636348	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	14336	2.0364504169211757	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	3776	0.536386493742631	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	3382	0.4804181996391892	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT	3275	0.4652186882963763	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2912	0.4136539909371138	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	2832	0.40228987030697333	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA	2671	0.37941957753881556	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	1376	0.1954628748384164	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGT	1059	0.15043254684148472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05625239711919542	0.0
2	0.0	0.0	0.0	0.5660752588888731	0.0
3	0.0	0.0	0.0	0.6657954174183559	0.0
4	0.0	0.0	0.0	1.352472406494595	0.0
5	0.0	0.0	0.0	4.241231870676307	0.0
6	0.0	0.0	0.0	4.560847763399009	0.0
7	0.0	0.0	0.0	5.111865562452945	0.0
8	0.0	0.0	0.0	5.755927099166158	0.0
9	0.0	0.0	0.0	5.883347301731608	0.0
10	0.0	0.0	0.0	9.005213290339077	0.0
11	0.0	0.0	0.0	9.178658181456596	0.0
12	0.0	0.0	0.0	11.752347401167663	0.0
13	0.0	0.0	0.0	11.94866258505334	0.0
14	0.0	0.0	0.0	12.109322840461951	0.0
15	0.0	0.0	0.0	12.640879582936773	0.0
16	0.0	0.0	0.0	12.88492407346904	0.0
17	0.0	0.0	0.0	13.00751452476668	0.0
18	0.0	0.0	0.0	13.100558262425956	0.0
19	0.0	0.0	0.0	13.786667045470688	0.0
20	0.0	0.0	0.0	13.936247283264912	0.0
21	0.0	0.0	0.0	14.048467974487549	0.0
22	0.0	0.0	0.0	14.253306248845831	0.0
23	0.0	0.0	0.0	14.358140261658878	0.0
24	0.0	0.0	0.0	14.457718368680483	0.0
25	0.0	0.0	0.0	14.54238106737503	0.0
26	0.0	0.0	0.0	14.652897140503146	0.0
27	0.0	0.0	0.0	14.813557395911758	0.0
28	0.0	0.0	0.0	14.909584215236444	0.0
29	0.0	0.0	0.0	15.024787988124494	0.0
30	0.0	0.0	0.0	15.16073128116255	0.0
31	0.0	0.0	0.0	15.263292469849567	0.0
32	0.0	0.0	0.0	15.391564981462277	0.0
33	0.0	0.0	0.0	15.516996462917454	0.0
34	0.0	0.0	0.0	15.67566799721579	0.0
35	0.0	0.0	0.0	15.847976476270295	0.0
36	0.0	0.0	0.0	15.984061820816228	0.0
37	0.0	0.0	0.0	16.126965637740245	0.0
38	0.0	0.0	0.0	16.309501825361878	0.0
39	0.0	0.0	0.0	16.61889000951745	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAT	20	7.0314226E-4	45.0	17
CGATGAA	655	0.0	44.312977	19
CGTTTTT	15555	0.0	44.175507	1
CCGATGA	665	0.0	42.63158	18
GATACCT	2195	0.0	42.23235	5
TGATACC	2220	0.0	41.85811	4
ATACCTG	2240	0.0	41.58482	6
TACGGCT	1825	0.0	40.931503	7
CGGCTGT	1820	0.0	40.796703	9
CGACCAC	1695	0.0	40.75221	12
ACGGCTG	1840	0.0	40.597824	8
TGGGACG	140	0.0	40.17857	6
GCGACCA	1745	0.0	40.100285	11
GAATCTG	2100	0.0	40.071426	1
TACCTGT	2345	0.0	39.626865	7
CCGGTGA	80	0.0	39.375	18
ATGATAC	6950	0.0	39.269783	3
AATCTGT	2150	0.0	39.139534	2
CACCGGT	190	0.0	39.078945	16
ACCACCG	1095	0.0	39.041096	14
>>END_MODULE