##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514923
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.630401050254115	31.0	28.0	31.0	16.0	33.0
2	28.98181475676946	31.0	30.0	33.0	16.0	34.0
3	29.1781217774308	31.0	30.0	33.0	16.0	34.0
4	32.04458336489145	35.0	30.0	37.0	19.0	37.0
5	33.54485622122142	35.0	33.0	37.0	28.0	37.0
6	32.79630352499306	35.0	33.0	37.0	19.0	37.0
7	31.781971285027083	35.0	30.0	35.0	23.0	37.0
8	32.93097997176277	35.0	32.0	35.0	27.0	37.0
9	32.59089223048883	35.0	31.0	37.0	25.0	39.0
10	31.460581484998727	35.0	27.0	39.0	15.0	39.0
11	31.780456495437182	35.0	27.0	39.0	15.0	39.0
12	32.75808033434125	35.0	31.0	39.0	21.0	39.0
13	32.72778260050532	35.0	31.0	39.0	21.0	39.0
14	33.049337473758214	36.0	31.0	39.0	18.0	41.0
15	33.659116023172395	36.0	31.0	39.0	22.0	41.0
16	33.92053763378214	36.0	32.0	40.0	24.0	41.0
17	31.909262161527064	34.0	27.0	39.0	17.0	41.0
18	32.68331964196589	35.0	30.0	39.0	21.0	39.0
19	32.512222215748764	36.0	30.0	37.0	18.0	39.0
20	32.287081757855056	34.0	30.0	37.0	23.0	39.0
21	32.63309659890896	35.0	31.0	37.0	23.0	39.0
22	32.98156229183781	35.0	31.0	37.0	25.0	39.0
23	34.048488803180284	35.0	32.0	38.0	27.0	40.0
24	32.24893624872068	35.0	30.0	37.0	21.0	39.0
25	31.43962301159591	34.0	29.0	37.0	18.0	39.0
26	30.865447843658178	33.0	26.0	37.0	19.0	39.0
27	32.42216020647747	34.0	30.0	37.0	22.0	39.0
28	32.43481646770488	35.0	31.0	38.0	21.0	39.0
29	32.72208854527764	35.0	31.0	38.0	22.0	40.0
30	31.650722535214	35.0	30.0	38.0	18.0	40.0
31	31.939678359677078	35.0	30.0	37.0	21.0	40.0
32	31.696024454141686	35.0	30.0	37.0	18.0	40.0
33	31.815521932405428	35.0	30.0	38.0	16.0	40.0
34	31.068674345484666	35.0	30.0	38.0	12.0	40.0
35	30.61232844522385	35.0	28.0	38.0	10.0	40.0
36	29.966381381293903	35.0	25.0	38.0	8.0	40.0
37	30.14504110323291	35.0	26.0	38.0	8.0	40.0
38	29.951493718478297	35.0	25.0	38.0	8.0	40.0
39	29.618191457751937	34.0	24.0	38.0	8.0	40.0
40	29.23062283098638	34.0	23.0	38.0	8.0	40.0
41	28.903597236868425	33.0	23.0	38.0	8.0	40.0
42	29.324524249256687	34.0	23.0	38.0	8.0	40.0
43	29.43623415539799	34.0	23.0	38.0	8.0	40.0
44	29.48181378575049	34.0	23.0	38.0	7.0	40.0
45	29.623520409847686	35.0	23.0	38.0	7.0	40.0
46	29.3713370736984	34.0	23.0	38.0	7.0	40.0
47	29.466467801982045	34.0	23.0	38.0	7.0	40.0
48	29.179621030717215	34.0	23.0	38.0	7.0	40.0
49	28.993012547507103	34.0	23.0	37.0	7.0	40.0
50	29.20423441951515	34.0	24.0	38.0	7.0	40.0
51	27.65336759088252	32.0	21.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	8.0
10	30.0
11	35.0
12	63.0
13	94.0
14	121.0
15	258.0
16	492.0
17	930.0
18	1780.0
19	2762.0
20	4169.0
21	5979.0
22	8232.0
23	11332.0
24	15164.0
25	20791.0
26	26164.0
27	28099.0
28	28630.0
29	30032.0
30	33169.0
31	37506.0
32	41984.0
33	46303.0
34	51109.0
35	53789.0
36	39987.0
37	21209.0
38	4610.0
39	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.922501034135202	2.5038695105870197	37.54639043119069	38.027239024087095
2	37.73729276027678	10.384076842557043	37.397436121517195	14.481194275648981
3	13.87527843968904	10.379221747717619	61.07204378130322	14.673456031290117
4	12.677817848493852	2.98996160591001	66.60024896926336	17.73197157633277
5	18.298658245990175	3.5488801238243393	60.75238433707564	17.400077293109845
6	15.237423847837444	9.572499189199162	62.30698570465876	12.88309125830464
7	57.23982032265018	1.2611594354884128	38.66869415427161	2.830326087589795
8	58.46000275769387	6.7771297844532095	31.456353668412557	3.3065137894403627
9	54.026135946539576	3.3902156244720087	31.553649768994585	11.029998659993824
10	27.180180337642717	24.443848886144142	36.95057319249674	11.4253975837164
11	25.03286899206289	16.57917785766027	45.330078477752984	13.057874672523853
12	20.97226187216341	15.231209326442983	47.2571627214166	16.539366079977004
13	19.059742913018063	15.347537398795549	52.12954169846754	13.463177989718853
14	16.05036092775036	17.829073473121223	48.57347603428085	17.54708956484756
15	13.550569696828457	15.845087517939577	53.976031367796736	16.628311417435228
16	16.90932430674101	16.47896870017459	46.48617366091629	20.12553333216811
17	17.222380821986977	16.56927346418785	49.51245137622518	16.695894337599988
18	17.11129625206099	15.564657240014526	48.97683731354008	18.347209194384405
19	16.78153821056741	18.48839535231481	45.85947801904363	18.870588418074156
20	18.08969496410143	18.817959190014818	49.078211693787225	14.014134152096528
21	18.59831469947934	20.3282820926624	46.50190416819602	14.571499039662243
22	16.54091971032562	15.826249749962615	49.16715703124545	18.465673508466317
23	16.09580461544736	20.438395643620503	47.79802028652828	15.667779454403863
24	16.929715705066585	17.740516543250155	46.64483816026862	18.68492959141464
25	16.200674663978887	20.929925445163647	44.680855195825394	18.18854469503207
26	15.880821015957725	16.77998458021879	47.26298883522391	20.07620556859958
27	16.18727460222208	18.014149688400014	46.129032884528364	19.66954282484954
28	13.884211814193579	19.235108938618005	47.92871943960553	18.95195980758288
29	18.16232718289919	17.1078005837766	44.94341872474137	19.786453508582838
30	16.6226795074215	19.23802199552166	46.39217902482507	17.74711947223177
31	19.219572635131854	18.597926291892186	43.52903249612078	18.653468576855182
32	21.222978969671193	19.869378528440173	42.562091807901375	16.345550693987256
33	18.449943001186586	19.88530323951348	41.29530046239923	20.3694532969007
34	20.066301175127155	20.519572829335647	41.077986417386676	18.33613957815052
35	16.72483070284295	22.268960601876397	40.81115040501201	20.19505829026864
36	18.206799851628304	24.05835435589399	38.236979121150156	19.497866671327557
37	20.32381540541013	22.717377161245466	37.92644725522069	19.03236017812372
38	19.57613080013905	26.21013238872608	36.44234186470599	17.771394946428885
39	23.453215335108357	21.787917805186407	36.37611837109626	18.38274848860898
40	20.009205259815545	22.446268665412113	39.35928284423108	18.185243230541264
41	19.84471464665591	22.38859013871977	38.18493250447154	19.58176271015278
42	18.32176849742583	21.530597778697008	41.072548711166526	19.075085012710637
43	19.686632758684308	20.86059469085669	38.22998778458138	21.222784765877616
44	21.113059622506665	21.265509600464537	36.50351605968271	21.11791471734609
45	18.727266018414404	21.619931523742387	37.05155916515673	22.60124329268648
46	21.00566492465864	22.993534955711826	35.73272120297598	20.268078916653558
47	16.801346997512248	22.214389335881286	41.06749941253353	19.916764254072937
48	19.584675767056435	22.90517222963433	36.33688920479373	21.17326279851551
49	18.5078157316725	20.932450094480146	39.52571549532649	21.034018678520866
50	18.48548229541116	20.81126692728816	38.62907658038192	22.074174196918765
51	18.419841413182166	20.423636155308657	37.41472025914554	23.741802172363634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	525.0
2	959.0
3	23357.5
4	45756.0
5	30522.5
6	15289.0
7	14251.0
8	13213.0
9	12675.5
10	12138.0
11	11386.0
12	10634.0
13	9681.0
14	8728.0
15	7973.0
16	7218.0
17	6604.0
18	5990.0
19	5494.5
20	4999.0
21	4794.0
22	4589.0
23	4518.0
24	4447.0
25	4587.5
26	5185.5
27	5643.0
28	6043.5
29	6444.0
30	7276.0
31	8108.0
32	8967.5
33	9827.0
34	10592.0
35	11357.0
36	11949.0
37	12541.0
38	13343.0
39	14145.0
40	14934.0
41	15723.0
42	16454.0
43	17185.0
44	18820.5
45	20456.0
46	33679.5
47	46903.0
48	40275.0
49	33647.0
50	31783.0
51	29919.0
52	27516.0
53	25113.0
54	23969.5
55	22826.0
56	21510.5
57	20195.0
58	19719.0
59	19243.0
60	17179.5
61	15116.0
62	13846.0
63	12576.0
64	11017.5
65	9459.0
66	7944.5
67	6430.0
68	5436.5
69	4443.0
70	3742.5
71	3042.0
72	2685.5
73	2329.0
74	1924.0
75	1252.5
76	986.0
77	727.0
78	468.0
79	366.5
80	265.0
81	205.0
82	145.0
83	100.0
84	55.0
85	40.5
86	26.0
87	15.0
88	4.0
89	2.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.930708094172175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3262979866879	35.97582328923708
2	7.554111828497439	6.939314106108982
3	3.665067626204775	5.050174538538361
4	2.4428619198986405	4.488095110289339
5	1.7860057506723859	4.10162543943231
6	1.3788866176773529	3.7999943238899334
7	1.0660433081212308	3.4274886800743323
8	0.8355899945451675	3.0703392100692004
9	0.628794974189854	2.5992898569537037
>10	2.2116467711416012	14.240254385216039
>50	0.06204042806905927	2.014876943079698
>100	0.03791359492849647	3.2682879188974363
>500	0.0012925089180169252	0.4317876143760115
>1k	0.0021541815300282086	2.0481218188779646
>5k	8.616726120112836E-4	2.628521943976426
>10k+	4.308363060056418E-4	5.9160048209831535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29896	5.805916612775114	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	7894	1.5330447464960781	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	5389	1.0465642435859341	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	4893	0.9502391619717899	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGAGTTT	1543	0.2996564534891625	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	1527	0.29654919279193204	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1306	0.25363015441143627	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC	1081	0.20993430085663295	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTA	832	0.1615775562559839	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT	685	0.13302959860017907	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCCAGAGTTTCGTATGCCG	665	0.129145522728641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9420379357690373E-4	0.0	0.0	0.08409024261879931	0.0
2	1.9420379357690373E-4	0.0	0.0	0.522408204721871	0.0
3	1.9420379357690373E-4	0.0	0.0	0.6626233436843956	0.0
4	1.9420379357690373E-4	0.0	0.0	1.2322230702454542	0.0
5	1.9420379357690373E-4	0.0	0.0	2.604272871866279	0.0
6	1.9420379357690373E-4	0.0	0.0	2.9544223116854367	0.0
7	1.9420379357690373E-4	0.0	0.0	3.3430241026328207	0.0
8	1.9420379357690373E-4	0.0	0.0	3.845235112822694	0.0
9	1.9420379357690373E-4	0.0	0.0	4.074589793037017	0.0
10	1.9420379357690373E-4	0.0	0.0	6.296281191556796	0.0
11	1.9420379357690373E-4	0.0	0.0	6.74508615851302	0.0
12	1.9420379357690373E-4	0.0	0.0	8.382806749747049	0.0
13	1.9420379357690373E-4	0.0	0.0	8.569630799168031	0.0
14	1.9420379357690373E-4	0.0	0.0	8.712953198827785	0.0
15	1.9420379357690373E-4	0.0	0.0	9.06213161967906	0.0
16	1.9420379357690373E-4	0.0	0.0	9.266239806728384	0.0
17	1.9420379357690373E-4	0.0	0.0	9.442576851296213	0.0
18	1.9420379357690373E-4	0.0	0.0	9.61658345034112	0.0
19	1.9420379357690373E-4	0.0	0.0	10.162684517879372	0.0
20	1.9420379357690373E-4	0.0	0.0	10.372230411148852	0.0
21	1.9420379357690373E-4	0.0	0.0	10.573425541294524	0.0
22	3.8840758715380746E-4	0.0	0.0	10.863954416485571	0.0
23	3.8840758715380746E-4	0.0	0.0	11.081268461498126	0.0
24	3.8840758715380746E-4	0.0	0.0	11.257605506065955	0.0
25	3.8840758715380746E-4	0.0	0.0	11.419377266115516	0.0
26	3.8840758715380746E-4	0.0	0.0	11.56522431509177	0.0
27	3.8840758715380746E-4	0.0	0.0	11.736317857233024	0.0
28	3.8840758715380746E-4	0.0	0.0	11.901585285566968	0.0
29	3.8840758715380746E-4	0.0	0.0	12.077339718754066	0.0
30	3.8840758715380746E-4	0.0	0.0	12.27911746028047	0.0
31	3.8840758715380746E-4	0.0	0.0	12.44904577966026	0.0
32	3.8840758715380746E-4	0.0	0.0	12.62771326975101	0.0
33	3.8840758715380746E-4	0.0	0.0	12.803467702938109	0.0
34	3.8840758715380746E-4	0.0	0.0	13.005828055845242	0.0
35	3.8840758715380746E-4	0.0	0.0	13.246058148499873	0.0
36	3.8840758715380746E-4	0.0	0.0	13.4375430889667	0.0
37	3.8840758715380746E-4	0.0	0.0	13.612909114566644	0.0
38	3.8840758715380746E-4	0.0	0.0	13.83585506959293	0.0
39	3.8840758715380746E-4	0.0	0.0	14.213775651893584	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	30	2.1631586E-6	45.000004	2
GACACGA	80	0.0	45.000004	25
GGCAACG	30	2.1631586E-6	45.000004	8
CACGCGA	30	2.1631586E-6	45.000004	15
CCGAGAA	20	7.0296536E-4	45.000004	21
CGTTTTT	13550	0.0	44.41882	1
AACACGT	75	0.0	42.0	41
TGATACC	1155	0.0	41.688313	4
GAATGAT	3135	0.0	41.483253	1
AATGATA	3030	0.0	41.435646	2
CGACCAC	670	0.0	41.30597	12
TACGGCT	790	0.0	41.297466	7
ATGATAC	3095	0.0	40.856216	3
GATACCT	1175	0.0	40.78723	5
CGGCGAC	1125	0.0	40.6	9
CGTTCAT	50	1.0786607E-9	40.500004	17
ATACGGC	1940	0.0	40.476807	6
GCGACCA	690	0.0	40.43478	11
ACGGCGA	1130	0.0	40.22124	8
ATACCTG	1175	0.0	40.212765	6
>>END_MODULE