##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933480.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403944
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.254490721486146	31.0	28.0	31.0	25.0	34.0
2	28.185055354207513	31.0	25.0	33.0	16.0	34.0
3	28.21594825025251	31.0	25.0	33.0	16.0	34.0
4	32.91839215336779	35.0	32.0	37.0	28.0	37.0
5	34.173920147347154	35.0	33.0	37.0	30.0	37.0
6	33.33938120135464	35.0	32.0	37.0	28.0	37.0
7	33.48354969995841	35.0	32.0	37.0	29.0	37.0
8	33.21959974649951	35.0	32.0	37.0	28.0	37.0
9	34.57499306834611	37.0	34.0	39.0	28.0	39.0
10	34.529632820390944	37.0	32.0	39.0	27.0	39.0
11	34.717079595191414	37.0	34.0	39.0	27.0	39.0
12	34.742506387023944	37.0	33.0	39.0	27.0	39.0
13	34.77427564216822	37.0	33.0	39.0	27.0	39.0
14	35.5913715762581	38.0	34.0	40.0	27.0	41.0
15	35.82033895787535	38.0	34.0	40.0	27.0	41.0
16	35.90980680490365	38.0	34.0	40.0	29.0	41.0
17	35.13111470896956	37.0	33.0	40.0	27.0	41.0
18	34.97091923632979	37.0	33.0	39.0	27.0	40.0
19	34.422501633889844	37.0	33.0	38.0	27.0	40.0
20	33.57783752203276	35.0	32.0	38.0	25.0	40.0
21	34.22669726496742	35.0	33.0	38.0	27.0	40.0
22	34.8538287485394	35.0	33.0	39.0	29.0	40.0
23	35.083600697126336	35.0	33.0	39.0	29.0	40.0
24	34.705684451310084	35.0	33.0	39.0	27.0	40.0
25	33.71812429445666	35.0	33.0	38.0	24.0	40.0
26	33.798165092191	35.0	32.0	38.0	25.0	40.0
27	34.41030192303884	35.0	33.0	39.0	27.0	40.0
28	34.07062117521241	35.0	33.0	39.0	25.0	40.0
29	34.04804626383855	35.0	33.0	39.0	24.0	40.0
30	33.501725486701126	35.0	33.0	39.0	22.0	40.0
31	33.48481967797517	35.0	33.0	39.0	23.0	40.0
32	33.02339433188759	35.0	32.0	39.0	20.0	40.0
33	32.55131404353079	35.0	31.0	39.0	15.0	40.0
34	32.088485037529956	35.0	31.0	39.0	12.0	40.0
35	31.690548689917414	35.0	30.0	40.0	10.0	41.0
36	31.340628899055314	35.0	29.0	40.0	8.0	41.0
37	31.08197670964292	35.0	28.0	40.0	8.0	40.0
38	30.957729289208405	35.0	27.0	39.0	8.0	40.0
39	30.59956578139544	35.0	26.0	39.0	7.0	40.0
40	30.317808904204544	35.0	24.0	39.0	8.0	40.0
41	29.840086744697285	35.0	23.0	39.0	7.0	40.0
42	30.121276711623395	35.0	23.0	39.0	7.0	40.0
43	30.08893807062365	35.0	23.0	39.0	7.0	40.0
44	30.207724338027052	35.0	23.0	39.0	7.0	40.0
45	30.321274731150854	35.0	24.0	39.0	7.0	40.0
46	30.078721307904065	35.0	23.0	39.0	7.0	40.0
47	29.87096231160755	35.0	23.0	39.0	7.0	40.0
48	29.706818271839662	35.0	23.0	38.0	7.0	40.0
49	29.997915552650863	35.0	24.0	39.0	7.0	40.0
50	29.910920820707823	35.0	24.0	39.0	7.0	40.0
51	28.639935238547917	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	13.0
10	32.0
11	31.0
12	37.0
13	44.0
14	72.0
15	125.0
16	200.0
17	461.0
18	837.0
19	1564.0
20	2463.0
21	3644.0
22	4919.0
23	7054.0
24	9593.0
25	13876.0
26	17909.0
27	19222.0
28	18027.0
29	17647.0
30	18642.0
31	21271.0
32	24917.0
33	29308.0
34	33018.0
35	38439.0
36	41277.0
37	41396.0
38	32837.0
39	5057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.94650743667439	2.2834848394827008	30.32276751232844	35.44724021151447
2	33.76111540213495	21.44703226189769	31.34840472936843	13.443447606598935
3	11.44737884459232	21.55174974748975	53.8554849187016	13.145386489216326
4	10.95770700889232	2.8593072307052463	70.95141900857544	15.231566751826985
5	25.510961915513043	3.383142217733151	53.38982631255818	17.71606955419563
6	13.362743350563445	18.86523874596479	57.162626502683544	10.609391400788228
7	51.62869110569782	1.4405462143267382	43.94668567920306	2.984077000772384
8	50.12848315608104	14.806755391836493	31.34023528013784	3.724526171944626
9	47.29343671399996	3.106866298298774	29.915037728001902	19.684659259699362
10	29.80833976986909	19.837403204404573	36.66721129661537	13.687045729110967
11	27.11217396470798	15.302121086091141	43.901134810765846	13.684570138435031
12	20.72515002079496	13.526379894242766	45.08298179945735	20.66548828550492
13	19.367288535044462	14.740904679856614	53.91564177212683	11.976165012972096
14	15.743766462677995	22.09836016873626	44.12047214465372	18.037401223932033
15	11.512734438437011	15.462539361891746	57.12400728813895	15.900718911532291
16	14.245786544669558	17.333343235696034	43.71695086447626	24.703919355158142
17	15.267957934763235	18.051759649852457	50.10075654051056	16.579525874873745
18	14.402243875388669	17.059542906937594	48.37254668964015	20.165666528033587
19	14.762442318737252	20.06812825540174	44.51978492068208	20.649644505178934
20	16.480007129701146	19.357881290475905	51.5108529895233	12.651258590299646
21	16.768661002515202	24.686095102291404	45.15229833838354	13.392945556809854
22	14.528003881726178	14.470075059909293	49.32985760402432	21.672063454340208
23	14.812449250391143	23.58817063751411	46.845354801655674	14.75402531043907
24	16.68325312419543	17.692303883706654	43.6582793654566	21.966163626641315
25	13.884597865050601	28.139048977085935	41.093072307052466	16.883280850811
26	14.859980591369101	16.007169310597508	47.85391044303171	21.278939655001682
27	17.32245063672192	20.208989364862457	42.599716792426676	19.86884320598895
28	12.359634008674469	19.43388192422712	49.6442576198681	18.56222644723031
29	21.767621254431308	17.494504188699423	42.69725506505852	18.040619491810748
30	14.613164200978353	25.78154397639277	45.62909710256867	13.976194720060207
31	18.89073732992692	16.92313786069356	43.25079714019765	20.935327669181866
32	22.081278593072305	25.03861921454459	38.70189927316658	14.178202919216526
33	16.475798625552056	18.139395559780564	40.864575287663634	24.520230527003744
34	24.03031113223615	19.73837957736716	37.99784128493059	18.233468005466104
35	18.826371972352604	25.963004772938824	35.86932842176143	19.341294832947142
36	20.69717584615689	20.027776127383994	38.35407878319767	20.920969243261442
37	16.967698492860396	29.618461965024856	36.624878696056875	16.78896084605787
38	18.900887251698258	21.899570237458658	39.90800705048225	19.29153546036084
39	19.73565642762363	25.059909294357634	33.159546867882675	22.044887410136056
40	22.425138137959717	21.0487097221397	38.929901174420216	17.596250965480365
41	17.333838353831226	23.18242132572832	32.500544629948706	26.98319569049175
42	20.545174578654468	21.798318578812907	39.62207632741172	18.03443051512091
43	20.66449804923455	20.378567326164024	33.27317647990811	25.683758144693325
44	20.919236329788287	22.28353435129622	34.991236409007186	21.805992909908305
45	17.622492226645278	20.867991602796426	36.421632701562594	25.087883468995702
46	23.404481809359716	25.507496088566732	31.71330679500129	19.374715307072268
47	15.385796050937753	20.853880735943598	44.48685956469214	19.273463648426514
48	20.262214564395066	24.014219792842574	32.08340760105361	23.64015804170875
49	19.003376705681976	20.062681955914684	39.34580040797734	21.588140930426
50	19.411106490008518	19.607173271542592	36.21888182520349	24.7628384132454
51	20.065652664725803	20.38104291683996	33.42790089715406	26.125403521280177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	591.0
2	1137.0
3	11897.0
4	22657.0
5	16808.5
6	10960.0
7	10673.0
8	10386.0
9	10125.5
10	9865.0
11	9409.5
12	8954.0
13	8401.0
14	7848.0
15	7143.5
16	6439.0
17	5957.5
18	5476.0
19	5022.5
20	4569.0
21	4374.5
22	4180.0
23	3997.5
24	3815.0
25	3999.0
26	4128.0
27	4073.0
28	4555.5
29	5038.0
30	5598.0
31	6158.0
32	6637.5
33	7117.0
34	7943.5
35	8770.0
36	9158.5
37	9547.0
38	9963.5
39	10380.0
40	10996.5
41	11613.0
42	12609.5
43	13606.0
44	15115.5
45	16625.0
46	45440.0
47	74255.0
48	50329.0
49	26403.0
50	24252.0
51	22101.0
52	19009.0
53	15917.0
54	15482.0
55	15047.0
56	14183.5
57	13320.0
58	12412.5
59	11505.0
60	10245.0
61	8985.0
62	7949.0
63	6913.0
64	6046.5
65	5180.0
66	4415.0
67	3650.0
68	3080.5
69	2511.0
70	2110.0
71	1709.0
72	1442.0
73	1175.0
74	984.5
75	674.0
76	554.0
77	392.0
78	230.0
79	176.0
80	122.0
81	101.0
82	80.0
83	51.5
84	23.0
85	16.0
86	9.0
87	8.5
88	8.0
89	8.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	403944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.98826961818319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.73273756737616	35.15792760847567
2	5.1230896701425035	4.302193406961453
3	2.0560476434472856	2.5898964840268444
4	1.3575852880872836	2.28010628423551
5	1.0797202580107688	2.2667792652785237
6	0.9219759179778377	2.322730405531524
7	0.8487263081817619	2.494558434198674
8	0.6998817490147647	2.350945886278205
9	0.6371141024842946	2.4076186841391625
>10	3.444383563589609	22.05636559943212
>50	0.06245471582659301	1.7619798313300925
>100	0.027955920417617828	2.202037728254773
>500	0.002379227269584496	0.7065401761634063
>1k	0.003568840904376744	3.9108210033731985
>5k	0.0	0.0
>10k+	0.002379227269584496	13.189499202320839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG	16736	4.143148555245282	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	12806	3.1702414196027173	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	11966	2.962291802824154	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11303	2.7981601410096446	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC	2847	0.7048006654387736	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT	2747	0.6800447586794209	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	2632	0.6515754659061652	No Hit
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	2545	0.6300378270255284	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA	2484	0.6149367239023231	No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	2404	0.5951319984948409	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATATCGCTCGT	882	0.21834709761749155	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTT	781	0.1933436317905452	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTAT	640	0.1584378032598578	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATG	526	0.13021606955419565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0915968550096053	0.0
2	0.0	0.0	0.0	0.8402154754124334	0.0
3	0.0	0.0	0.0	0.9578060325193591	0.0
4	2.4755906759352784E-4	0.0	0.0	1.6878577228526725	0.0
5	2.4755906759352784E-4	0.0	0.0	5.132889707484206	0.0
6	2.4755906759352784E-4	0.0	0.0	5.429713029528846	0.0
7	2.4755906759352784E-4	0.0	0.0	6.240964094032837	0.0
8	2.4755906759352784E-4	0.0	0.0	7.1146000435703955	0.0
9	2.4755906759352784E-4	0.0	0.0	7.250262412611649	0.0
10	2.4755906759352784E-4	0.0	0.0	12.12247242191987	0.0
11	2.4755906759352784E-4	0.0	0.0	12.333145188441963	0.0
12	2.4755906759352784E-4	0.0	0.0	16.14109876616561	0.0
13	2.4755906759352784E-4	0.0	0.0	16.41341374051849	0.0
14	2.4755906759352784E-4	0.0	0.0	16.64512902778603	0.0
15	2.4755906759352784E-4	0.0	0.0	17.450438674667776	0.0
16	2.4755906759352784E-4	0.0	0.0	17.81212247242192	0.0
17	2.4755906759352784E-4	0.0	0.0	17.986651615075356	0.0
18	2.4755906759352784E-4	0.0	0.0	18.125284692927732	0.0
19	2.4755906759352784E-4	0.0	0.0	19.149931673697346	0.0
20	2.4755906759352784E-4	0.0	0.0	19.371497039193553	0.0
21	2.4755906759352784E-4	0.0	0.0	19.507159408234806	0.0
22	2.4755906759352784E-4	0.0	0.0	19.786406036480304	0.0
23	2.4755906759352784E-4	0.0	0.0	19.930980531954923	0.0
24	2.4755906759352784E-4	0.0	0.0	20.059711247103557	0.0
25	2.4755906759352784E-4	0.0	0.0	20.161210464816904	0.0
26	2.4755906759352784E-4	0.0	0.0	20.288950943695166	0.0
27	2.4755906759352784E-4	0.0	0.0	20.481304339215338	0.0
28	2.4755906759352784E-4	0.0	0.0	20.591468124294458	0.0
29	2.4755906759352784E-4	0.0	0.0	20.71871348503753	0.0
30	2.4755906759352784E-4	0.0	0.0	20.853138058740814	0.0
31	2.4755906759352784E-4	0.0	0.0	20.960331135008815	0.0
32	2.4755906759352784E-4	0.0	0.0	21.103915394213058	0.0
33	2.4755906759352784E-4	0.0	0.0	21.229675400550573	0.0
34	2.4755906759352784E-4	0.0	0.0	21.429950686233735	0.0
35	2.4755906759352784E-4	0.0	0.0	21.582942190006534	0.0
36	2.4755906759352784E-4	0.0	0.0	21.727764244548748	0.0
37	2.4755906759352784E-4	0.0	0.0	21.890905670092884	0.0
38	2.4755906759352784E-4	0.0	0.0	22.053799536569425	0.0
39	2.4755906759352784E-4	0.0	0.0	22.330323015071396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	20	7.027847E-4	45.000004	35
CCATCGA	20	7.027847E-4	45.000004	15
CACAATT	20	7.027847E-4	45.000004	42
TCAAGCG	20	7.027847E-4	45.000004	17
CGTTTTT	9300	0.0	44.201614	1
CCGATGA	300	0.0	43.500004	18
GATACCT	1920	0.0	43.007816	5
CGATGAA	315	0.0	42.857143	19
TACGGCT	1590	0.0	42.73585	7
ACGGCTG	1595	0.0	42.460815	8
TGATACC	1945	0.0	42.455013	4
GCGACTG	315	0.0	42.142857	11
CGGCTGT	1600	0.0	41.90625	9
ATACCTG	2005	0.0	41.745636	6
TACGGGA	65	0.0	41.538464	4
CACCGGT	60	3.6379788E-12	41.250004	16
ATGATAC	5505	0.0	41.19891	3
ATACGGC	3575	0.0	41.16084	6
AATGATA	5390	0.0	41.159554	2
GAATGAT	5705	0.0	41.13497	1
>>END_MODULE