##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933479.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263007
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.20786899208006	31.0	26.0	31.0	16.0	33.0
2	28.246305231419697	31.0	28.0	33.0	16.0	34.0
3	28.4266160216268	31.0	28.0	33.0	16.0	34.0
4	31.62518488101077	35.0	28.0	35.0	19.0	37.0
5	33.515986266525225	35.0	33.0	37.0	28.0	37.0
6	32.21624139281464	35.0	32.0	37.0	17.0	37.0
7	31.948685776424202	35.0	31.0	35.0	25.0	37.0
8	32.47968685244119	35.0	32.0	35.0	25.0	37.0
9	32.77232164923367	35.0	31.0	37.0	25.0	39.0
10	31.627143003798377	35.0	28.0	39.0	15.0	39.0
11	32.145863037865915	35.0	27.0	39.0	17.0	39.0
12	32.944891200614435	35.0	31.0	39.0	22.0	39.0
13	33.02458489697993	35.0	31.0	39.0	23.0	39.0
14	33.251050352271996	36.0	31.0	39.0	18.0	41.0
15	33.83759747839411	36.0	31.0	39.0	23.0	41.0
16	34.09638526731228	36.0	32.0	40.0	25.0	41.0
17	32.00201895767033	34.0	27.0	39.0	18.0	41.0
18	32.810035474340985	36.0	30.0	39.0	22.0	39.0
19	32.67614550183075	36.0	30.0	37.0	20.0	39.0
20	32.16866851452623	34.0	30.0	36.0	23.0	39.0
21	32.667332048196435	35.0	31.0	37.0	23.0	39.0
22	32.983422494458324	35.0	31.0	37.0	25.0	39.0
23	33.88434908576578	35.0	32.0	38.0	27.0	40.0
24	32.30455463162578	35.0	31.0	37.0	21.0	39.0
25	31.501450531734896	34.0	29.0	37.0	18.0	39.0
26	30.85731938693647	33.0	26.0	37.0	19.0	39.0
27	32.487093499412566	35.0	30.0	37.0	22.0	39.0
28	32.38033588459623	35.0	31.0	37.0	21.0	39.0
29	32.54731242894676	35.0	31.0	38.0	21.0	40.0
30	31.621439733543212	35.0	30.0	38.0	18.0	40.0
31	31.897858231910178	35.0	30.0	37.0	20.0	40.0
32	31.743786287057	35.0	30.0	37.0	18.0	40.0
33	31.761291524560182	35.0	30.0	38.0	16.0	40.0
34	31.001467641545663	35.0	30.0	38.0	12.0	40.0
35	30.496458269171544	35.0	27.0	38.0	10.0	40.0
36	30.03999513320938	35.0	25.0	38.0	8.0	40.0
37	29.84660104103693	35.0	25.0	38.0	8.0	40.0
38	29.957495427878346	35.0	25.0	38.0	8.0	40.0
39	29.511149893348847	34.0	24.0	38.0	8.0	40.0
40	29.192158383617166	34.0	23.0	38.0	8.0	40.0
41	28.73437208895581	33.0	22.0	37.0	7.0	40.0
42	29.23116875216249	34.0	23.0	38.0	7.0	40.0
43	29.217811693224895	34.0	23.0	38.0	7.0	40.0
44	29.151117650861003	34.0	23.0	38.0	7.0	40.0
45	29.429783237708502	34.0	23.0	38.0	7.0	40.0
46	29.122909276178962	34.0	23.0	38.0	7.0	40.0
47	29.072697684852493	34.0	23.0	38.0	7.0	40.0
48	28.883501199587844	34.0	23.0	38.0	7.0	40.0
49	28.81957134220762	34.0	23.0	37.0	7.0	40.0
50	28.946233370214483	34.0	23.0	38.0	7.0	40.0
51	27.15790074028448	32.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	6.0
10	15.0
11	24.0
12	38.0
13	39.0
14	65.0
15	179.0
16	325.0
17	606.0
18	1044.0
19	1608.0
20	2300.0
21	3129.0
22	4352.0
23	5914.0
24	7951.0
25	10771.0
26	13311.0
27	14630.0
28	14721.0
29	15290.0
30	16752.0
31	19143.0
32	21747.0
33	23695.0
34	26073.0
35	27205.0
36	19220.0
37	10521.0
38	2281.0
39	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.92060287368777	2.358872577535959	32.91433307858726	38.806191470189006
2	38.47730288547453	15.135718821172059	33.06908181151072	13.31789648184269
3	12.051010049162189	15.014809491762577	59.887759641378366	13.046420817696866
4	11.731626914872988	2.874448208602813	70.01220499834605	15.381719878178146
5	21.233655377993742	3.383179915363469	58.579429444843676	16.803735261799115
6	13.6931716646325	13.507625272331156	61.07670138057162	11.722501682464726
7	51.59596512640348	1.490074408665929	43.865372404536764	3.04858806039383
8	52.0723783017182	10.486793127179125	33.412038462854596	4.02879010824807
9	48.39795138532435	2.9098084841848317	33.055013744881315	15.637226385609509
10	27.223229799967303	20.353070450596373	39.049911219093026	13.373788530343301
11	24.213043759291576	15.78513119422677	47.221937058709464	12.77988798777219
12	20.222275452744604	14.017497633142845	47.85195831289661	17.908268601215937
13	17.94552996688301	15.308337800895034	54.86089723847655	11.885234993745414
14	14.883634275893797	18.7572954332014	48.08845391947743	18.270616371427376
15	11.483344549764835	15.779047705954593	57.40607664434787	15.331531099932702
16	14.206465987597289	15.476774382430886	47.323075051234376	22.993684578737447
17	14.869566209264393	16.474086241050617	51.82637724471212	16.82997030497287
18	14.109130175242484	15.760417023121057	51.27658199211428	18.85387080952218
19	15.052831293463672	18.651594824472355	47.4002593086876	18.895314573376375
20	16.015543312535407	18.27669985969955	52.79555297007305	12.912203857692
21	16.66153372343702	21.558741782538107	47.699490888075225	14.080233605949651
22	14.817476340933892	14.706832897983704	50.62450809294049	19.851182668141913
23	14.572615937978837	21.553418730299953	48.36943503404852	15.504530297672686
24	15.466888713988602	16.65887219731794	47.210530518199135	20.66370857049432
25	13.92776618112826	23.175428790868686	44.8835962540921	18.01320877391096
26	14.579459862285034	16.430741387111368	49.67358283239609	19.3162159182075
27	15.098077237487976	19.134851924093272	46.783925903112845	18.983144935305905
28	12.576471348671328	18.154269658222024	51.86097708426012	17.408281908846533
29	19.27173041021722	17.133004064530603	46.603322345032645	16.991943180219536
30	14.495431680525614	22.529058161950065	48.555361644366876	14.420148513157443
31	17.209427885949804	17.123118396088316	46.11474219317356	19.552711524788315
32	20.16752405829503	22.354538092142036	42.95893265198264	14.519005197580292
33	15.883607660632606	18.08925237731315	43.74370263909326	22.283437322960985
34	21.566726360895338	19.36792556852099	41.54566228275293	17.519685787830742
35	17.06114285931553	24.80884539194774	39.352944978650754	18.777066770085966
36	19.52647648161456	19.550049998669238	41.427794697479534	19.49567882223667
37	16.25964327945644	27.550977730630745	39.538871588969116	16.650507400943702
38	17.90598729311387	21.778888014387448	42.59696509978822	17.71815959271046
39	18.00446375951971	23.486447128783645	37.522955662777036	20.98613344891961
40	21.33745489663773	20.339382601983978	40.826289794568204	17.496872706810084
41	17.04859566475417	21.495245373697276	36.61119285798477	24.844966103563785
42	19.441307645804105	21.167877661050845	41.17114753599714	18.21966715714791
43	19.397202355830835	19.940914120156496	37.33969057857776	23.322192945434914
44	19.760310562076295	21.97013767694396	37.68759006414278	20.581961696836967
45	17.069127437672762	20.812754033162616	39.44875991893752	22.669358610227107
46	20.518465287996136	24.573110221400952	35.73174858463843	19.17667590596448
47	15.477154600447896	20.390331816263448	45.1265555669621	19.005958016326563
48	19.13295083400822	22.44579041622466	36.080408506237475	22.34085024352964
49	18.165295980715342	19.820004790747014	40.40234670560099	21.61235252293665
50	18.17556186717464	19.367165132486967	38.64041641477223	23.816856585566164
51	19.282376514693524	19.86981335097545	36.46062652324843	24.387183611082595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	285.5
2	515.0
3	12039.5
4	23564.0
5	15654.0
6	7744.0
7	7383.0
8	7022.0
9	7014.0
10	7006.0
11	6651.0
12	6296.0
13	5800.0
14	5304.0
15	4775.0
16	4246.0
17	3863.0
18	3480.0
19	3194.5
20	2909.0
21	2695.0
22	2481.0
23	2395.5
24	2310.0
25	2339.0
26	2385.5
27	2403.0
28	2758.0
29	3113.0
30	3507.5
31	3902.0
32	4255.0
33	4608.0
34	5130.5
35	5653.0
36	5890.5
37	6128.0
38	6438.5
39	6749.0
40	7387.5
41	8026.0
42	8613.5
43	9201.0
44	10089.0
45	10977.0
46	22073.5
47	33170.0
48	26304.0
49	19438.0
50	17970.0
51	16502.0
52	13895.0
53	11288.0
54	10475.0
55	9662.0
56	8954.0
57	8246.0
58	7645.0
59	7044.0
60	6348.0
61	5652.0
62	5060.0
63	4468.0
64	4041.0
65	3614.0
66	3099.5
67	2585.0
68	2172.5
69	1760.0
70	1490.0
71	1220.0
72	1045.5
73	871.0
74	721.5
75	491.0
76	410.0
77	316.0
78	222.0
79	164.0
80	106.0
81	92.0
82	78.0
83	49.5
84	21.0
85	13.5
86	6.0
87	4.5
88	3.0
89	3.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	263007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.0056743803767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18106817411164	32.058674242576544
2	7.510218841326894	6.159231766656413
3	3.4058731317363145	4.189803738625595
4	2.2264865812269075	3.6519433504827488
5	1.6500987912738931	3.383170686521522
6	1.2528649577798112	3.0824743500782006
7	1.001469741145512	2.874615947504911
8	0.8428668696234383	2.764985952142891
9	0.7301515839080772	2.6946322288245823
>10	3.0981563848906215	18.672227819682625
>50	0.04987373414697282	1.3865819375385562
>100	0.03989898731757825	3.01407737395918
>500	0.0049873734146972815	1.619315012010367
>1k	0.003989898731757826	4.401068332718249
>5k	9.974746829394564E-4	3.1989549656309872
>10k+	9.974746829394564E-4	6.848242295046621
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16743	6.365990258814404	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG	7821	2.9736851110426716	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	4884	1.8569847950814997	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	3486	1.325440007300186	No Hit
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	1265	0.48097579151885694	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT	1125	0.4277452691373233	No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	937	0.35626428193926396	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC	901	0.34257643332686966	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA	818	0.3110183379149604	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	663	0.2520845452782626	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGATATCGCTCGTATGCCG	640	0.24333953088701063	No Hit
GAATCTTTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	374	0.14220153836209684	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	352	0.13383674198785583	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATATCGCTCGT	296	0.11254453303524241	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	292	0.1110236609671986	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	278	0.10570060872904523	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTT	268	0.1018984285589357	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1315554338857901	0.0
2	0.0	0.0	0.0	0.9056793165200926	0.0
3	0.0	0.0	0.0	1.1368518708627526	0.0
4	0.0	0.0	0.0	1.7919675141726266	0.0
5	0.0	0.0	0.0	3.619295303927272	0.0
6	0.0	0.0	0.0	3.943621272437616	0.0
7	0.0	0.0	0.0	4.404065291037881	0.0
8	0.0	0.0	0.0	5.0173569524765504	0.0
9	0.0	0.0	0.0	5.173246339451041	0.0
10	0.0	0.0	0.0	8.960978224914166	0.0
11	0.0	0.0	0.0	9.542331572923915	0.0
12	0.0	0.0	0.0	12.09511533913546	0.0
13	0.0	0.0	0.0	12.357085552856008	0.0
14	0.0	0.0	0.0	12.520579300170718	0.0
15	0.0	0.0	0.0	13.046040599679856	0.0
16	0.0	0.0	0.0	13.363902861901014	0.0
17	0.0	0.0	0.0	13.686327740326304	0.0
18	0.0	0.0	0.0	13.94829795404685	0.0
19	0.0	0.0	0.0	14.729645979004362	0.0
20	0.0	0.0	0.0	15.038763226834266	0.0
21	0.0	0.0	0.0	15.341416768374986	0.0
22	0.0	0.0	0.0	15.78551141224378	0.0
23	0.0	0.0	0.0	16.077899067325205	0.0
24	0.0	0.0	0.0	16.2756124361709	0.0
25	0.0	0.0	0.0	16.47066427889752	0.0
26	0.0	0.0	0.0	16.65811176128392	0.0
27	0.0	0.0	0.0	16.871414068827065	0.0
28	0.0	0.0	0.0	17.04935610078819	0.0
29	0.0	0.0	0.0	17.25619470204215	0.0
30	0.0	0.0	0.0	17.45466850692187	0.0
31	0.0	0.0	0.0	17.628428140695874	0.0
32	0.0	0.0	0.0	17.812833878946186	0.0
33	0.0	0.0	0.0	17.98545285866916	0.0
34	0.0	0.0	0.0	18.197994730178284	0.0
35	0.0	0.0	0.0	18.414718999874527	0.0
36	0.0	0.0	0.0	18.59380168588669	0.0
37	0.0	0.0	0.0	18.785051348443197	0.0
38	0.0	0.0	0.0	19.00101518210542	0.0
39	0.0	0.0	0.0	19.335226819058047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGTA	35	1.2071541E-7	45.000004	5
TTTAGCG	20	7.023356E-4	45.0	1
AAATCCC	20	7.023356E-4	45.0	25
ACACGCG	20	7.023356E-4	45.0	36
GAGTCAT	20	7.023356E-4	45.0	9
TTTGTCG	40	6.7811925E-9	45.0	1
TGAACGA	20	7.023356E-4	45.0	25
TCGTGCG	20	7.023356E-4	45.0	1
CGTGTCT	25	3.8825772E-5	45.0	20
ATGAACG	20	7.023356E-4	45.0	24
TTACACG	20	7.023356E-4	45.0	34
CGTTTTT	7160	0.0	44.46578	1
TGATACC	910	0.0	42.774723	4
CTAATCC	90	0.0	42.500004	11
GAATGAT	2440	0.0	42.141396	1
AATGATA	2330	0.0	42.00644	2
TACGGCT	645	0.0	41.860462	7
ACGGCTG	635	0.0	41.811024	8
CGGGACT	70	0.0	41.785717	6
ATGATAC	2395	0.0	41.7119	3
>>END_MODULE