##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933477.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	315942
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.279785530255552	31.0	26.0	31.0	16.0	33.0
2	28.341280994612934	31.0	28.0	33.0	16.0	34.0
3	28.533395370036274	31.0	28.0	33.0	16.0	34.0
4	31.75077071107988	35.0	28.0	37.0	19.0	37.0
5	33.564423216919565	35.0	33.0	37.0	28.0	37.0
6	32.26677681346576	35.0	32.0	37.0	17.0	37.0
7	31.990074127529738	35.0	31.0	35.0	25.0	37.0
8	32.553512986560825	35.0	32.0	35.0	25.0	37.0
9	32.82689227769654	35.0	31.0	37.0	25.0	39.0
10	31.680662906482837	35.0	28.0	39.0	15.0	39.0
11	32.46375917098708	35.0	30.0	39.0	17.0	39.0
12	33.32055883674852	35.0	31.0	39.0	25.0	39.0
13	33.36275962043666	35.0	31.0	39.0	25.0	39.0
14	33.37265700666578	36.0	31.0	39.0	18.0	41.0
15	33.942989536054085	36.0	32.0	40.0	25.0	41.0
16	34.12614973634401	36.0	32.0	40.0	25.0	41.0
17	32.0289072044869	35.0	27.0	39.0	17.0	41.0
18	32.836761177684515	36.0	30.0	39.0	22.0	39.0
19	32.671123180836986	36.0	30.0	37.0	19.0	39.0
20	32.21390001962386	34.0	30.0	36.0	23.0	39.0
21	32.625124231662774	35.0	31.0	37.0	23.0	39.0
22	33.011821790075395	35.0	31.0	37.0	25.0	39.0
23	33.912243386444345	35.0	32.0	38.0	27.0	40.0
24	32.28839470535731	35.0	31.0	37.0	21.0	39.0
25	31.283184888365586	34.0	28.0	37.0	18.0	39.0
26	30.720819011084313	33.0	25.0	37.0	18.0	39.0
27	32.421064625785746	34.0	30.0	37.0	22.0	39.0
28	32.28578979686145	35.0	31.0	37.0	21.0	39.0
29	32.41637389141045	35.0	30.0	38.0	21.0	40.0
30	31.33883117787442	34.0	29.0	38.0	18.0	40.0
31	31.767080666704647	35.0	30.0	37.0	20.0	40.0
32	31.5794639522444	35.0	30.0	37.0	18.0	40.0
33	31.451193573503996	35.0	30.0	38.0	15.0	40.0
34	30.690667907400726	35.0	28.0	38.0	10.0	40.0
35	30.17520937387242	35.0	26.0	38.0	8.0	40.0
36	29.640522627570885	35.0	24.0	38.0	8.0	40.0
37	29.644602490330502	35.0	24.0	38.0	7.0	40.0
38	29.569993859632465	35.0	23.0	38.0	8.0	40.0
39	29.177225566717944	34.0	23.0	38.0	7.0	40.0
40	28.935342562875466	34.0	22.0	38.0	7.0	40.0
41	28.55822904203936	33.0	21.0	38.0	7.0	40.0
42	29.009552386197466	34.0	22.0	38.0	7.0	40.0
43	29.06997170366713	34.0	22.0	38.0	7.0	40.0
44	29.01535408397744	34.0	22.0	38.0	7.0	40.0
45	29.26440929031278	35.0	23.0	38.0	7.0	40.0
46	28.91441467104722	34.0	22.0	38.0	7.0	40.0
47	28.933139626893542	34.0	22.0	38.0	7.0	40.0
48	28.73735369150034	34.0	22.0	38.0	7.0	40.0
49	28.526533351058106	34.0	20.0	37.0	7.0	40.0
50	28.72017332295168	34.0	22.0	38.0	7.0	40.0
51	27.12081647897399	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	18.0
10	14.0
11	27.0
12	29.0
13	53.0
14	84.0
15	169.0
16	384.0
17	763.0
18	1262.0
19	1917.0
20	3011.0
21	4044.0
22	5553.0
23	7509.0
24	10181.0
25	13519.0
26	16801.0
27	17381.0
28	17086.0
29	17591.0
30	19702.0
31	22425.0
32	25738.0
33	28638.0
34	31379.0
35	32497.0
36	23402.0
37	12189.0
38	2546.0
39	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.79600686201898	2.4972938070911748	34.172094878173844	38.534604452716
2	38.954618252717275	14.474175639832628	33.54951225224883	13.021693855201272
3	12.168056162206987	14.29471232061581	59.8540238398187	13.683207677358503
4	11.214400111412855	2.8498901697146946	70.2056073583126	15.730102360559851
5	20.429699121990748	3.1945736875755677	59.78312475074539	16.5926024396883
6	13.107785606218863	13.00428559672345	62.40512499129587	11.482803805761817
7	51.62276620392351	1.3201790201999102	44.17741230985434	2.879642466022245
8	52.132669920428434	10.138253223692956	33.89134714599515	3.83772970988346
9	48.4585778402365	2.680555291794063	33.85020035322939	15.010666514740048
10	27.008438257654888	19.1725063461015	40.40994866146318	13.409106734780435
11	21.74354786638054	17.4418089396155	47.656531895094666	13.158111298909295
12	19.12534579131613	14.758405023706883	49.09255496261972	17.023694222357268
13	16.585955650087676	16.981281374429482	54.23938571003538	12.193377265447456
14	14.646675655658317	19.974868805033836	46.866196960201556	18.51225857910629
15	11.303340486545	17.10915294579385	55.607674826392184	15.979831741268969
16	11.986377246456628	16.393515265460117	48.01577504731881	23.604332440764445
17	12.437092884136963	18.0422356002051	52.19280754062454	17.32786397503339
18	12.61655620335378	16.54575839869343	51.485399218843966	19.352286179108823
19	13.497097568541061	20.241373416639764	46.86271530850599	19.398813706313184
20	16.219116166891393	17.594685100429825	52.913192927815864	13.273005804862917
21	16.01179963411006	22.441777288236448	46.50568775281539	15.040735324838103
22	14.408024257616905	16.68027675965842	49.189091668724004	19.72260731400067
23	14.402010495597292	22.765254382133428	47.500490596375286	15.332244525893993
24	14.808097688816302	17.344322692139695	48.68140354875262	19.16617607029138
25	12.831785580897758	24.035107709642908	45.36022434497471	17.772882364484623
26	13.980414126643497	18.068506244817087	49.44166967354768	18.50940995499174
27	14.921726139607902	20.432547746105296	45.84354090307715	18.80218521120965
28	12.179450658665198	19.323166910382287	50.73272942502105	17.764653005931468
29	16.184616163726254	16.80973089997531	46.665527217020845	20.340125719277587
30	14.96382247374518	22.25566717941901	47.33685296668376	15.443657380152054
31	15.399661963271742	19.262079748814656	43.61401776275392	21.72424052515968
32	17.033189636072443	24.578878401731963	42.699609421982515	15.688322540213075
33	14.581157301023605	22.301561679042354	41.18192579650695	21.935355223427084
34	15.889308797184293	21.343157921390635	40.11938900177881	22.648144279646264
35	17.309506175184055	22.591488311145717	40.67708630065012	19.42191921302011
36	15.376556456564813	23.64896088522577	36.617796937412564	24.356685720796854
37	16.881263016629635	26.580195099100468	39.38222838369067	17.15631350057922
38	15.766501446468023	26.251337270764886	37.49390710953276	20.488254173234328
39	15.92159320381589	26.392502421330498	36.992549265371494	20.69335510948212
40	16.48087307163973	24.824493103164507	41.265801950991005	17.428831874204757
41	15.851960169904602	26.582410695634007	36.836824480442615	20.728804654018777
42	15.540510600046845	23.14665349969298	42.24066442574903	19.072171474511144
43	17.243987820549343	24.982749998417432	36.22785194750936	21.545410233523874
44	19.26777699704376	22.962442473618573	36.72541162618455	21.04436890315311
45	15.507593165834235	22.471213070753493	37.66450804261542	24.356685720796854
46	19.19561185280843	26.750479518392616	35.115305973881284	18.938602654917673
47	15.02047844224573	23.27357552968583	42.430256186262035	19.275689841806408
48	17.533914452652702	23.677447126371295	35.827462002519454	22.961176418456553
49	15.296478467566832	22.15628184920017	40.736590893265216	21.81064878996778
50	17.257597913541094	22.29744699976578	38.14877414208937	22.296180944603755
51	16.999322660488318	21.324800121541294	36.42693912173753	25.248938096232855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	298.0
2	529.0
3	13378.5
4	26228.0
5	18165.0
6	10102.0
7	9520.5
8	8939.0
9	8610.0
10	8281.0
11	7966.5
12	7652.0
13	7100.0
14	6548.0
15	6085.0
16	5622.0
17	5231.0
18	4840.0
19	4530.5
20	4221.0
21	3916.0
22	3611.0
23	3380.5
24	3150.0
25	3164.0
26	3371.0
27	3564.0
28	3845.5
29	4127.0
30	4579.0
31	5031.0
32	5557.0
33	6083.0
34	6650.5
35	7218.0
36	8249.0
37	9280.0
38	9375.5
39	9471.0
40	10310.0
41	11149.0
42	11796.0
43	12443.0
44	13512.5
45	14582.0
46	26381.5
47	38181.0
48	30807.0
49	23433.0
50	21281.0
51	19129.0
52	16031.0
53	12933.0
54	11624.5
55	10316.0
56	9409.0
57	8502.0
58	7733.5
59	6965.0
60	6498.0
61	6031.0
62	5288.5
63	4546.0
64	3838.5
65	3131.0
66	2643.0
67	2155.0
68	1891.0
69	1627.0
70	1368.0
71	1109.0
72	971.5
73	834.0
74	654.5
75	396.5
76	318.0
77	237.0
78	156.0
79	127.5
80	99.0
81	71.5
82	44.0
83	31.0
84	18.0
85	12.0
86	6.0
87	6.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	315942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.210216477555605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9223711771364	39.14783094104827
2	6.530668812077274	6.16628576722778
3	2.629192547598552	3.7237424799991072
4	1.594874905847046	3.0117755823864063
5	1.1315626897849642	2.6710659771336633
6	0.8550585485957006	2.422049950811252
7	0.723601463195645	2.391296720465967
8	0.5993347867602096	2.26357800203833
9	0.476091087132851	2.0228726957919014
>10	2.445439172247506	17.55785383390172
>50	0.05032263638640947	1.6357308554842285
>100	0.03400178134216856	3.066313130663369
>500	0.002720142507373485	1.0347321976890418
>1k	0.002720142507373485	2.6354690073935294
>5k	0.0013600712536867424	4.315504251112659
>10k+	6.800356268433712E-4	5.933898606852796
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18483	5.850124389919669	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCG	8114	2.5681928961644855	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGC	5328	1.6863854758151813	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	4108	1.3002386513980415	No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	1502	0.4754037133397902	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCAACTAT	1403	0.44406884807971087	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	1196	0.37855049344499936	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTC	987	0.31239911122927627	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTA	919	0.29087617347487826	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGCAACTATCGTATGCCG	673	0.2130137810104386	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	644	0.2038348810857689	No Hit
GAATCTTTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	380	0.12027524039222388	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGCAACTATCGT	370	0.11711010248716537	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	354	0.11204588183907173	TruSeq Adapter, Index 13 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1651379050585233E-4	0.0	0.0	0.13705047128903405	0.0
2	3.1651379050585233E-4	0.0	0.0	0.8735780617961525	0.0
3	3.1651379050585233E-4	0.0	0.0	1.0808945945774857	0.0
4	3.1651379050585233E-4	0.0	0.0	1.7658304372321503	0.0
5	3.1651379050585233E-4	0.0	0.0	3.529128764140254	0.0
6	3.1651379050585233E-4	0.0	0.0	3.8399453064170004	0.0
7	3.1651379050585233E-4	0.0	0.0	4.2599591064182665	0.0
8	3.1651379050585233E-4	0.0	0.0	4.871780263466079	0.0
9	3.1651379050585233E-4	0.0	0.0	5.0455463344537925	0.0
10	3.1651379050585233E-4	0.0	0.0	8.351216362496913	0.0
11	3.1651379050585233E-4	0.0	0.0	8.941198067999823	0.0
12	3.1651379050585233E-4	0.0	0.0	11.440390957834033	0.0
13	3.1651379050585233E-4	0.0	0.0	11.70847813839249	0.0
14	3.1651379050585233E-4	0.0	0.0	11.859138702673276	0.0
15	3.1651379050585233E-4	0.0	0.0	12.393413981047154	0.0
16	3.1651379050585233E-4	0.0	0.0	12.720372726639699	0.0
17	3.1651379050585233E-4	0.0	0.0	13.00491862430446	0.0
18	3.1651379050585233E-4	0.0	0.0	13.300859018427433	0.0
19	3.1651379050585233E-4	0.0	0.0	14.09784074292117	3.1651379050585233E-4
20	3.1651379050585233E-4	0.0	0.0	14.386817833653012	3.1651379050585233E-4
21	3.1651379050585233E-4	0.0	0.0	14.735299516999955	3.1651379050585233E-4
22	3.1651379050585233E-4	0.0	0.0	15.21798304752138	3.1651379050585233E-4
23	3.1651379050585233E-4	0.0	0.0	15.568996841192371	3.1651379050585233E-4
24	3.1651379050585233E-4	0.0	0.0	15.843414297560944	3.1651379050585233E-4
25	3.1651379050585233E-4	0.0	0.0	16.096625329965626	3.1651379050585233E-4
26	3.1651379050585233E-4	0.0	0.0	16.31375379025264	3.1651379050585233E-4
27	3.1651379050585233E-4	0.0	0.0	16.539111609092807	3.1651379050585233E-4
28	3.1651379050585233E-4	0.0	0.0	16.757506124541845	3.1651379050585233E-4
29	3.1651379050585233E-4	0.0	0.0	17.002487798393375	3.1651379050585233E-4
30	6.330275810117047E-4	0.0	0.0	17.24810249982592	3.1651379050585233E-4
31	6.330275810117047E-4	0.0	0.0	17.48675389786733	3.1651379050585233E-4
32	6.330275810117047E-4	0.0	0.0	17.71052914775497	3.1651379050585233E-4
33	6.330275810117047E-4	0.0	0.0	17.937469535547663	3.1651379050585233E-4
34	6.330275810117047E-4	0.0	0.0	18.14921726139608	3.1651379050585233E-4
35	6.330275810117047E-4	0.0	0.0	18.394831962828622	3.1651379050585233E-4
36	6.330275810117047E-4	0.0	0.0	18.610694367953613	3.1651379050585233E-4
37	6.330275810117047E-4	0.0	0.0	18.837318241955803	3.1651379050585233E-4
38	6.330275810117047E-4	0.0	0.0	19.09179532952251	3.1651379050585233E-4
39	6.330275810117047E-4	0.0	0.0	19.355134803223375	3.1651379050585233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTG	120	0.0	45.000004	11
CGGAATA	20	7.025512E-4	45.0	20
CGGGTGA	20	7.025512E-4	45.0	6
CGGGTCG	25	3.8843624E-5	45.0	6
ACCGGTC	25	3.8843624E-5	45.0	17
GATGTCG	20	7.025512E-4	45.0	10
GGGTCGT	40	6.7884685E-9	45.0	7
AATGGTA	20	7.025512E-4	45.0	34
TACGGGA	25	3.8843624E-5	45.0	4
TCGTCGG	35	1.208191E-7	45.0	2
ATTGCGG	25	3.8843624E-5	45.0	2
TATACGG	45	3.8380676E-10	45.0	27
CGTTTTT	8825	0.0	44.464592	1
GATACCT	1110	0.0	43.378376	5
CACCGGT	125	0.0	43.199997	16
TGATACC	1140	0.0	42.82895	4
TACGGCT	780	0.0	42.692307	7
ACGGCTG	800	0.0	42.1875	8
CTACGGC	80	0.0	42.1875	6
GAATGAT	3055	0.0	41.98036	1
>>END_MODULE