##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	916782
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.108871029317765	31.0	28.0	31.0	25.0	34.0
2	27.977743891132242	31.0	25.0	33.0	16.0	34.0
3	27.94149863326287	31.0	25.0	33.0	16.0	34.0
4	32.90511266582459	35.0	32.0	37.0	28.0	37.0
5	34.17303132042296	35.0	33.0	37.0	30.0	37.0
6	33.353906381233486	35.0	32.0	37.0	28.0	37.0
7	33.84865867785362	35.0	33.0	37.0	30.0	37.0
8	33.273695382326444	35.0	32.0	37.0	28.0	37.0
9	34.85304467147043	37.0	34.0	39.0	28.0	39.0
10	34.895893462131674	37.0	34.0	39.0	28.0	39.0
11	35.29888566747602	37.0	34.0	39.0	30.0	39.0
12	35.39228518884533	37.0	34.0	39.0	30.0	39.0
13	35.3945299973167	37.0	34.0	39.0	30.0	39.0
14	36.24196155683685	38.0	34.0	40.0	30.0	41.0
15	36.355464003438115	38.0	34.0	41.0	29.0	41.0
16	36.4501179124372	38.0	34.0	41.0	30.0	41.0
17	35.57794764731419	38.0	33.0	40.0	27.0	41.0
18	35.33406305970231	37.0	33.0	39.0	27.0	40.0
19	34.59555052346141	37.0	33.0	37.0	27.0	40.0
20	33.248375295326476	35.0	32.0	37.0	25.0	39.0
21	33.88994221090728	35.0	33.0	37.0	27.0	39.0
22	34.3255681285191	35.0	33.0	38.0	28.0	40.0
23	34.526911523132	35.0	33.0	38.0	28.0	40.0
24	34.02266187599669	35.0	33.0	38.0	26.0	40.0
25	33.160015139913305	35.0	32.0	37.0	23.0	40.0
26	32.99410437814006	35.0	31.0	38.0	23.0	40.0
27	33.78775870381399	35.0	33.0	38.0	25.0	40.0
28	33.38364300346211	35.0	33.0	38.0	23.0	40.0
29	33.1005364415968	35.0	32.0	38.0	22.0	40.0
30	32.43773328882984	35.0	31.0	38.0	20.0	40.0
31	32.40634741956103	35.0	31.0	38.0	19.0	40.0
32	31.509343551684044	35.0	30.0	38.0	15.0	40.0
33	30.588596852905052	35.0	25.0	38.0	10.0	40.0
34	29.563755614748107	35.0	22.0	38.0	7.0	40.0
35	28.54299931717682	35.0	18.0	38.0	7.0	40.0
36	28.06778274442561	34.0	16.0	38.0	7.0	40.0
37	27.762086297505842	34.0	15.0	38.0	7.0	40.0
38	27.645096653293805	34.0	15.0	38.0	7.0	40.0
39	27.405334092510543	34.0	15.0	38.0	7.0	40.0
40	27.01852457836214	33.0	15.0	38.0	7.0	40.0
41	26.718021296229637	33.0	14.0	38.0	7.0	40.0
42	26.57461643007825	33.0	12.0	38.0	7.0	40.0
43	26.710740394117686	33.0	12.0	38.0	7.0	40.0
44	26.908138466941978	33.0	12.0	38.0	7.0	40.0
45	26.94804980900585	33.0	12.0	38.0	7.0	40.0
46	26.74286689747399	33.0	10.0	38.0	7.0	40.0
47	26.6749565327417	33.0	10.0	38.0	7.0	40.0
48	26.507204548082314	33.0	10.0	38.0	7.0	40.0
49	26.508037897777225	33.0	10.0	38.0	7.0	40.0
50	26.494131647436358	33.0	10.0	38.0	7.0	40.0
51	25.339442746476262	31.0	10.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	30.0
10	38.0
11	62.0
12	84.0
13	130.0
14	199.0
15	289.0
16	596.0
17	1268.0
18	2667.0
19	4789.0
20	7743.0
21	11396.0
22	16358.0
23	23670.0
24	34088.0
25	49053.0
26	60931.0
27	60885.0
28	52765.0
29	46477.0
30	45685.0
31	48028.0
32	52760.0
33	59574.0
34	65753.0
35	73280.0
36	73607.0
37	68883.0
38	48949.0
39	6729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.286589396388674	2.0087654426024946	23.910046226911085	43.79459893409774
2	43.86528095010591	22.063151327142112	23.526748998126052	10.54481872462592
3	8.79151204975665	21.979380048910212	58.32542523740649	10.903682663926647
4	8.46777096408961	2.215793940107899	77.2402817681848	12.07615332761769
5	22.69143591388138	2.768924346245891	60.03793704501179	14.501702694860938
6	10.32273757556322	17.864552314508792	63.38540678154676	8.427303328381228
7	39.34424977802793	1.0007831741897202	57.035042136516644	2.61992491126571
8	37.143290335106926	14.165417732896152	44.72153685390856	3.9697550780883573
9	34.66407499274637	2.385299885905264	43.13239134276196	19.818233778586404
10	25.7303262934918	11.531094633184335	50.136455558682435	12.60212351464143
11	21.44741061670059	12.307942346162992	53.42491453802539	12.819732499111023
12	14.840496432085274	10.569361091295422	56.19722027701242	18.392922199606886
13	15.348250729180984	12.02281458405597	63.445290156220345	9.183644530542702
14	13.249714763160709	19.6259307010827	52.89272695144538	14.23162758431121
15	9.000940245336404	12.8799431053402	64.89078101446145	13.22833563486194
16	10.496279377212904	15.484488133493022	51.9988394187495	22.02039307054458
17	11.35711652279386	16.614527772142125	58.204022330281354	13.824333374782663
18	11.401729091539755	15.09813674352245	55.94296135831637	17.55717280662142
19	11.266582459079693	17.24772083221529	52.96548143397231	18.520215274732706
20	13.204665885674022	16.491488707239014	59.96867303241119	10.335172374675768
21	13.012362808170316	21.96149139053777	54.28215213649482	10.743993664797085
22	10.696108780495255	12.454323928698425	58.21296665946757	18.636600631338748
23	12.364880636836238	20.2626142310822	55.70179170184406	11.670713430237505
24	14.683861594141245	15.474016723714035	52.06843066290568	17.773691019239035
25	10.570342785962202	25.62343065199797	50.23757010936079	13.568656452679045
26	11.984419414866347	13.302617197981636	56.30291606946908	18.41004731768294
27	15.244518326057884	16.661758193332766	51.91626798955477	16.17745549105458
28	10.567397701961863	16.308348113291927	57.407322569596694	15.716931615149512
29	19.848339081700995	12.682295245761807	52.24109984707379	15.228265825463414
30	12.826386207408087	18.73858779949868	57.311661878178235	11.123364114914997
31	17.133517019313206	13.290836861980274	52.79881149499008	16.776834623716432
32	22.610936951205414	18.386377568495018	47.52133004356543	11.481355436734141
33	19.04018621657057	14.805264501266386	45.667017895203	20.48753138696004
34	26.02101699204391	17.05530867752639	43.80637927009911	13.11729506033059
35	15.46725393823177	18.395867283607224	48.87388714001802	17.26299163814298
36	20.34943967049964	18.02609562578672	45.938729163530695	15.685735540182943
37	19.639456272047227	19.68352345486713	46.598755211162526	14.078265061923117
38	20.90213376789684	19.923602339487466	41.84331716809449	17.33094672452121
39	27.179198544474044	19.413012035576614	38.30594405213017	15.101845367819175
40	20.6918329548355	19.60367895530235	44.16447966910345	15.540008420758697
41	20.870283229819083	22.66187599669278	40.46992632926912	15.997914444219019
42	17.04680065708096	20.59464518282427	46.13048685510841	16.228067304986354
43	21.236019031787272	19.393923528167	39.992386412473195	19.377671027572532
44	20.600317196454554	20.141429478327456	38.86038338449053	20.39786994072746
45	16.084303574895667	19.261503825336884	39.856149008161154	24.79804359160629
46	23.994144736698583	22.312829004059854	36.14545224491755	17.547574014324017
47	15.762525878562188	19.0781450770194	47.18940816900856	17.96992087540986
48	19.861755575480323	22.65260443595097	35.33195459771243	22.15368539085628
49	16.15269497001468	19.40930341127989	44.22730812777738	20.21069349092805
50	18.42586350953662	18.68394012971459	40.69407994485058	22.196116415898217
51	18.39564912923683	19.537360026702093	36.597577177562385	25.469413666498685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	1393.5
2	2744.0
3	35881.0
4	69018.0
5	52155.0
6	35292.0
7	33718.5
8	32145.0
9	31732.0
10	31319.0
11	30292.5
12	29266.0
13	28025.5
14	26785.0
15	25513.5
16	24242.0
17	22823.5
18	21405.0
19	19974.0
20	18543.0
21	17308.5
22	16074.0
23	14999.0
24	13924.0
25	13252.5
26	12265.0
27	11949.0
28	11944.5
29	11940.0
30	11889.0
31	11838.0
32	12383.5
33	12929.0
34	14139.0
35	15349.0
36	16088.0
37	16827.0
38	18034.0
39	19241.0
40	20316.0
41	21391.0
42	22744.0
43	24097.0
44	28085.0
45	32073.0
46	97634.0
47	163195.0
48	110465.5
49	57736.0
50	51322.5
51	44909.0
52	37313.5
53	29718.0
54	27406.5
55	25095.0
56	23126.5
57	21158.0
58	19448.0
59	17738.0
60	15659.0
61	13580.0
62	11806.5
63	10033.0
64	8800.5
65	7568.0
66	6484.5
67	5401.0
68	4473.0
69	3545.0
70	2939.5
71	2334.0
72	1885.5
73	1437.0
74	1248.0
75	823.0
76	587.0
77	472.0
78	357.0
79	260.5
80	164.0
81	121.0
82	78.0
83	60.0
84	42.0
85	29.0
86	16.0
87	15.0
88	14.0
89	7.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	916782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93933453944277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04994064655911	42.1122683240115
2	5.799024852800451	5.676008345474883
3	2.4127632211789476	3.542370793372202
4	1.3749544427145575	2.6915742179400333
5	0.9549927409113608	2.336835461510024
6	0.7136320428373935	2.0954806369491092
7	0.5280650412297477	1.8090206197929084
8	0.424593539985501	1.6623460237309362
9	0.3262834981822586	1.43712875450171
>10	1.3066240903929418	9.637095461857013
>50	0.057931270434621776	1.9665570256675886
>100	0.04311125286269084	4.075499923759918
>500	0.004266217730512861	1.3238122189718122
>1k	0.0015717644270310542	1.2428252840185272
>5k	0.0013472266517409036	4.190222257311625
>10k+	8.981511011606024E-4	14.200954651130177
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37367	4.075887179285806	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	32605	3.556461623373932	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	31628	3.449893213435691	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	27632	3.014020781385324	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	7945	0.8666182363964388	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT	6862	0.7484876448272326	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	6428	0.7011481464513919	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC	6089	0.6641709806693412	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	5432	0.5925072699943934	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA	5376	0.5863989476233172	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	3229	0.3522102310036628	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGT	2249	0.24531458950982896	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTAT	1434	0.1564166835736304	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATG	1253	0.13667371305283044	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGC	1091	0.11900320905078852	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.11376750416129461	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1011	0.11027703423496535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08300773793551793	0.0
2	0.0	0.0	0.0	0.9011957041041382	0.0
3	0.0	0.0	0.0	1.038632957453353	0.0
4	0.0	0.0	0.0	2.1051896743173404	0.0
5	0.0	0.0	0.0	6.407848321629351	0.0
6	0.0	0.0	0.0	6.93152788776394	0.0
7	0.0	0.0	0.0	7.758660183118779	0.0
8	0.0	0.0	0.0	8.693560737449033	0.0
9	0.0	0.0	0.0	8.875392405173749	0.0
10	0.0	0.0	0.0	13.090898381512726	0.0
11	0.0	0.0	0.0	13.345484531764367	0.0
12	0.0	0.0	0.0	17.283716303330564	0.0
13	0.0	0.0	0.0	17.608330006479186	0.0
14	0.0	0.0	0.0	17.847754427988335	0.0
15	0.0	0.0	0.0	18.66027038052667	0.0
16	0.0	0.0	0.0	19.053384555979502	0.0
17	0.0	0.0	0.0	19.25135964711349	0.0
18	0.0	0.0	0.0	19.412030340909833	0.0
19	0.0	0.0	0.0	20.47280596695834	0.0
20	0.0	0.0	0.0	20.689106025205557	0.0
21	0.0	0.0	0.0	20.833196986851835	0.0
22	0.0	0.0	0.0	21.17002733474261	0.0
23	0.0	0.0	0.0	21.312809370166516	0.0
24	0.0	0.0	0.0	21.457882026479577	0.0
25	0.0	0.0	0.0	21.56674105730697	0.0
26	0.0	0.0	0.0	21.702869384433814	0.0
27	0.0	0.0	0.0	21.905534794531306	0.0
28	0.0	0.0	0.0	22.021701996766954	0.0
29	0.0	0.0	0.0	22.166556498709618	0.0
30	0.0	0.0	0.0	22.31839194050494	0.0
31	0.0	0.0	0.0	22.453320418594608	0.0
32	0.0	0.0	0.0	22.611700491501797	0.0
33	0.0	0.0	0.0	22.7547006812961	0.0
34	0.0	0.0	0.0	22.986489699841403	0.0
35	0.0	0.0	0.0	23.15075994074927	0.0
36	0.0	0.0	0.0	23.31273956076799	0.0
37	0.0	0.0	0.0	23.497189080937453	0.0
38	0.0	0.0	0.0	23.689928467181947	0.0
39	0.0	0.0	0.0	23.95640403062015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTT	20	7.0325396E-4	45.0	9
CCGTGCG	20	7.0325396E-4	45.0	1
CTAGCGG	20	7.0325396E-4	45.0	2
CGTTTTT	31265	0.0	44.41708	1
CACCGGT	235	0.0	43.085106	16
GATACCT	3795	0.0	42.450592	5
TGATACC	3820	0.0	42.349476	4
CCGATGA	1235	0.0	42.085022	18
CGATGAA	1240	0.0	41.91532	19
TACGGCT	3915	0.0	41.666668	7
ACGGCTG	3910	0.0	41.662407	8
ATACCTG	3885	0.0	41.58301	6
CGGCTGT	3935	0.0	41.512074	9
GAATCTG	4000	0.0	41.175	1
AATCTGT	4035	0.0	40.92937	2
TACCTGT	3995	0.0	40.719646	7
CGCTTTT	1025	0.0	40.609756	1
CCGGTGA	145	0.0	40.344826	18
GCGACTA	90	0.0	40.0	11
GAATGAC	710	0.0	39.929577	1
>>END_MODULE