FastQCFastQC Report
Sat 14 Jan 2017
SRR2933475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567306
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429297.5671683359597814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG137512.4239123153994493No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC104581.8434495669004027No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC92091.6232861982774727No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT32980.5813441070603872No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT31940.5630118489844986No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC30420.5362185487197386No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC17660.31129584386556813No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA17330.30547887736071894No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT13940.24572276690181313No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTTGGGTTTCGTATGCCG11440.20165483883477345No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGC10350.1824412221975442No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT9790.1725700063105273No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGT8100.14278008693720848No Hit
GAATCTTTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC7050.12427155714905183No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6890.1214512097527613No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC6400.11281389585162153No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC6260.11034609187986731No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6120.10787828790811307No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT5890.10382403852594543TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG207.030262E-445.0000046
CGGGATA551.8189894E-1245.06
TCGTTGA253.8883E-544.99999624
GGCATCG253.8883E-544.99999623
GCATCGG253.8883E-544.99999624
GTACGGG302.1635515E-644.9999963
CGTTTTT208450.044.730151
GACCCGT1150.043.0434838
CACCGGT2200.042.95454416
GATACCT19150.042.297655
CGATGAA2150.041.86046619
TGATACC19300.041.8523334
TACGGCT18350.041.811997
ACGGCTG18350.041.6893738
GAATGAT61200.041.3970571
CCGATGA2400.041.24999618
CGACCAC13350.040.7865212
AATGATA59000.040.766952
ATGATAC60100.040.544933
GCCGATT501.0786607E-940.4999969
Produced by FastQC (version 0.11.5)