##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567306
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.08066193553391	31.0	25.0	31.0	16.0	33.0
2	28.06587273887461	31.0	28.0	33.0	16.0	34.0
3	28.1985172023564	31.0	28.0	33.0	16.0	34.0
4	31.677154833546624	35.0	28.0	37.0	19.0	37.0
5	33.60270295043592	35.0	33.0	37.0	28.0	37.0
6	32.25483425170896	35.0	32.0	37.0	17.0	37.0
7	32.56245483037373	35.0	32.0	37.0	26.0	37.0
8	32.55174808657056	35.0	32.0	37.0	23.0	37.0
9	33.29387667325923	35.0	32.0	38.0	25.0	39.0
10	32.39772715254202	35.0	28.0	39.0	17.0	39.0
11	33.15892305034673	37.0	31.0	39.0	17.0	39.0
12	33.80441595893574	37.0	31.0	39.0	25.0	39.0
13	33.92512153934561	37.0	32.0	39.0	26.0	39.0
14	34.21108185000688	37.0	32.0	40.0	23.0	41.0
15	34.5343271532471	37.0	32.0	40.0	25.0	41.0
16	34.69415976562913	37.0	32.0	40.0	25.0	41.0
17	32.75735317447727	36.0	29.0	40.0	18.0	41.0
18	33.2708626385055	36.0	30.0	39.0	23.0	39.0
19	32.935096755542865	36.0	31.0	37.0	23.0	39.0
20	32.00208176892189	34.0	30.0	35.0	23.0	39.0
21	32.49373882878024	35.0	31.0	36.0	24.0	39.0
22	32.491577737587825	34.0	30.0	37.0	24.0	39.0
23	33.414903068185424	35.0	32.0	37.0	26.0	39.0
24	31.869869171135154	34.0	30.0	36.0	21.0	39.0
25	31.164995610834364	34.0	28.0	36.0	18.0	39.0
26	29.934407533147894	33.0	25.0	36.0	18.0	39.0
27	31.94174043637825	34.0	30.0	37.0	22.0	39.0
28	31.864605697806827	35.0	30.0	37.0	21.0	39.0
29	31.67741042752941	34.0	30.0	37.0	20.0	39.0
30	30.900646212097175	34.0	27.0	37.0	18.0	39.0
31	31.30371792295516	34.0	30.0	36.0	18.0	39.0
32	30.87988316710911	35.0	29.0	37.0	15.0	39.0
33	30.408996203107318	35.0	26.0	37.0	12.0	40.0
34	29.068860544397555	34.0	22.0	37.0	8.0	39.0
35	28.27243498217893	34.0	20.0	37.0	7.0	39.0
36	27.620740834752322	33.0	18.0	37.0	7.0	39.0
37	27.357419100097655	33.0	15.0	37.0	7.0	39.0
38	27.44368647608169	33.0	16.0	37.0	7.0	40.0
39	27.17274980345704	33.0	15.0	37.0	7.0	40.0
40	26.702753011602205	33.0	15.0	37.0	7.0	39.0
41	26.333576588296264	32.0	15.0	37.0	7.0	39.0
42	26.419144165582594	32.0	15.0	37.0	7.0	39.0
43	26.532166062054692	33.0	15.0	37.0	7.0	39.0
44	26.61128209467201	33.0	15.0	37.0	7.0	40.0
45	26.782456029021375	33.0	15.0	37.0	7.0	40.0
46	26.492806351422338	33.0	12.0	37.0	7.0	40.0
47	26.5775137227528	33.0	13.0	37.0	7.0	40.0
48	26.374161387328883	32.0	13.0	37.0	7.0	39.0
49	26.16440157516402	32.0	12.0	36.0	7.0	39.0
50	26.2822092486242	32.0	12.0	37.0	7.0	39.0
51	24.7739403426017	29.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	26.0
11	31.0
12	44.0
13	85.0
14	155.0
15	359.0
16	849.0
17	1535.0
18	2607.0
19	4262.0
20	6534.0
21	9399.0
22	12728.0
23	18001.0
24	23652.0
25	32254.0
26	38637.0
27	38340.0
28	34521.0
29	33667.0
30	35064.0
31	38820.0
32	42337.0
33	45959.0
34	48538.0
35	48588.0
36	31985.0
37	15400.0
38	2857.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.9249963864299	2.0921689529107748	27.132799582588586	45.85003507807074
2	47.41920586068189	16.131153204796	25.450109817276743	10.999531117245366
3	9.488177456258175	15.798881027170522	63.42555869319203	11.28738282337927
4	9.202441010671489	2.4128071975265555	75.2992212315752	13.085530560226754
5	19.480492009603285	2.873052638258718	63.575213376907705	14.0712419752303
6	11.036195633397144	13.326141447472791	65.62507711887412	10.012585800255946
7	40.554304026398455	1.090064268666293	55.446443365661565	2.90918833927369
8	40.53614804003483	10.623014739840581	44.52676333407368	4.314073886050914
9	37.03204267185611	2.422502141701304	44.22639633636874	16.31905885007386
10	24.28830296171731	12.546315392398458	50.286265260723496	12.87911638516074
11	19.8723440259754	13.022249015522485	55.00893697581199	12.096469982690119
12	15.110187447338827	11.239613189354598	56.834935643197845	16.815263720108724
13	14.975515859165952	12.476159250915732	62.67058694954751	9.877737940370805
14	13.024364276069706	16.702978639393905	55.35813123781522	14.914525846721169
15	9.411499261421525	13.318032948708458	63.62597963004093	13.644488159829088
16	10.780249107183778	13.696664586660463	54.1746429616468	21.34844334450896
17	11.78993347505579	15.350622062872594	58.78450078088369	14.074943681187928
18	11.63093639058991	13.689261174745198	57.23013682210306	17.449665612561827
19	11.73158753829503	16.557025661635873	54.41560639231737	17.295780407751725
20	12.912431738779423	15.820033632642701	60.248261079558475	11.0192735490194
21	13.372500907799317	19.94990357937339	54.75827860096667	11.919316911860617
22	11.074270323247067	13.31785667699619	58.44500146305521	17.162871536701534
23	12.13648366137499	19.341766172048242	55.44379928997755	13.077950876599227
24	13.314154971038558	15.016410896412166	54.04684597025239	17.62258816229689
25	11.239789461066866	21.273527866794993	52.20128114280477	15.285401529333376
26	11.998815454093558	14.381832732246794	56.635748608334836	16.983603205324815
27	12.8976249149489	16.305838471653747	54.40344364417087	16.393092969226483
28	10.904696936045097	15.64305683352547	58.423143770733965	15.029102459695473
29	17.65308316851928	12.873299418655893	54.06887993428591	15.404737478538918
30	13.247347992088926	17.53550993643642	57.11661783940237	12.100524232072285
31	16.368062386084407	13.708122247957894	53.92028288084385	16.00353248511385
32	19.67791632734362	17.645679756604018	50.61395437383	12.062449542222362
33	17.282383757619343	14.611691045044473	49.08532608504053	19.02059911229566
34	23.159282644639752	16.84822653030287	46.389602789323575	13.602888035733802
35	15.034743154488053	18.152284657662708	49.52547655057412	17.28749563727512
36	19.364152679506297	17.125501933700686	47.38853458274723	16.12181080404579
37	19.033995762428038	19.08722981953302	47.49993830490071	14.378836113138235
38	20.581132581005665	19.04351443489052	44.5022615660684	15.873091418035418
39	24.71611440739213	18.62504538996591	41.27631295984883	15.382527242793131
40	20.59858348052021	18.63333016044251	45.28896221792119	15.479124141116083
41	19.952195111632875	20.26102315152669	44.02156860671313	15.765213130127304
42	17.02062026490113	19.412274856955506	47.215788304724434	16.35131657341893
43	19.805008231888962	18.512760309251092	43.18145762604309	18.500773832816858
44	19.981632487581656	19.037168653248866	41.91441655825957	19.066782300909914
45	16.632646226198915	18.423390551131135	42.49470303504634	22.449260187623608
46	21.624484845920897	21.032035620987617	39.58674859775853	17.75673093533296
47	16.37264545060338	18.072257300292964	47.85054274060207	17.704554508501584
48	19.22807091763528	20.695180378843165	39.63381314493412	20.44293555858743
49	16.201838161415534	18.12831170479424	45.29601308641192	20.37383704737831
50	17.85050748625962	17.454601220505335	42.95124676982087	21.743644523414172
51	18.0133825483954	18.0371792295516	40.23560477061762	23.71383345143538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	372.0
2	686.0
3	30591.5
4	60497.0
5	41365.5
6	22234.0
7	21103.0
8	19972.0
9	19582.0
10	19192.0
11	18376.0
12	17560.0
13	16702.5
14	15845.0
15	14983.5
16	14122.0
17	13169.0
18	12216.0
19	11191.0
20	10166.0
21	9441.5
22	8717.0
23	8263.5
24	7810.0
25	7253.5
26	6485.5
27	6274.0
28	6364.0
29	6454.0
30	6572.0
31	6690.0
32	6984.0
33	7278.0
34	8021.0
35	8764.0
36	9345.5
37	9927.0
38	10756.0
39	11585.0
40	12468.5
41	13352.0
42	14673.0
43	15994.0
44	18455.5
45	20917.0
46	45425.0
47	69933.0
48	57162.0
49	44391.0
50	39475.0
51	34559.0
52	28453.0
53	22347.0
54	19605.5
55	16864.0
56	15262.5
57	13661.0
58	12375.5
59	11090.0
60	9861.0
61	8632.0
62	7608.0
63	6584.0
64	5785.0
65	4986.0
66	4376.0
67	3766.0
68	3193.0
69	2620.0
70	2202.5
71	1785.0
72	1431.0
73	1077.0
74	972.0
75	689.0
76	511.0
77	410.0
78	309.0
79	226.5
80	144.0
81	110.5
82	77.0
83	60.0
84	43.0
85	36.5
86	30.0
87	23.5
88	17.0
89	10.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567306.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.865886110888184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.23123290995036	43.353621862881205
2	6.551823205889088	6.665285860188573
3	2.6113214693360534	3.9848154137449474
4	1.4736277128283373	2.9982951764229937
5	0.9625621253293045	2.448078772082744
6	0.7047269744134566	2.1507937211871417
7	0.5376566827999723	1.9143868515842926
8	0.40884031022176753	1.6636819725824494
9	0.28488170711319455	1.304168442218564
>10	1.1308785338262386	8.84524023443042
>50	0.049457833002114135	1.7599233857914436
>100	0.044158779408537394	4.714452716302551
>500	0.0045925130584878885	1.4941559054755187
>1k	0.002826161882146393	2.9839991540981914
>5k	3.5327023526829913E-4	1.6548023732440231
>10k+	0.0010598107058048974	12.064298157764927
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42929	7.5671683359597814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	13751	2.4239123153994493	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	10458	1.8434495669004027	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	9209	1.6232861982774727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT	3298	0.5813441070603872	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	3194	0.5630118489844986	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	3042	0.5362185487197386	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC	1766	0.31129584386556813	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA	1733	0.30547887736071894	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	1394	0.24572276690181313	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTTGGGTTTCGTATGCCG	1144	0.20165483883477345	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGC	1035	0.1824412221975442	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	979	0.1725700063105273	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGT	810	0.14278008693720848	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	705	0.12427155714905183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	689	0.1214512097527613	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	640	0.11281389585162153	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	626	0.11034609187986731	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	612	0.10787828790811307	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	589	0.10382403852594543	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5254342453631726E-4	0.0	0.0	0.1274444479698787	0.0
2	3.5254342453631726E-4	0.0	0.0	0.9104433938650394	0.0
3	3.5254342453631726E-4	0.0	0.0	1.1362474572805505	0.0
4	3.5254342453631726E-4	0.0	0.0	2.1029215273591326	0.0
5	3.5254342453631726E-4	0.0	0.0	4.402738557321798	0.0
6	3.5254342453631726E-4	0.0	0.0	5.038903166897582	0.0
7	3.5254342453631726E-4	0.0	0.0	5.697278012219155	0.0
8	3.5254342453631726E-4	0.0	0.0	6.478514240991634	0.0
9	3.5254342453631726E-4	0.0	0.0	6.830176306966611	0.0
10	3.5254342453631726E-4	0.0	0.0	10.301847680087995	0.0
11	3.5254342453631726E-4	0.0	0.0	11.04412786044921	0.0
12	3.5254342453631726E-4	0.0	0.0	14.095391199811036	0.0
13	3.5254342453631726E-4	0.0	0.0	14.48266015166418	0.0
14	3.5254342453631726E-4	0.0	0.0	14.72591511459424	0.0
15	3.5254342453631726E-4	0.0	0.0	15.343042379245064	0.0
16	3.5254342453631726E-4	0.0	0.0	15.709334997338297	0.0
17	3.5254342453631726E-4	0.0	0.0	16.047247869756358	0.0
18	3.5254342453631726E-4	0.0	0.0	16.382516666490396	0.0
19	3.5254342453631726E-4	0.0	0.0	17.390262045527457	0.0
20	3.5254342453631726E-4	0.0	0.0	17.70684604076107	0.0
21	3.5254342453631726E-4	0.0	0.0	18.00844694045189	0.0
22	3.5254342453631726E-4	0.0	0.0	18.4507126665327	0.0
23	3.5254342453631726E-4	0.0	0.0	18.783337387582716	0.0
24	3.5254342453631726E-4	0.0	0.0	19.05162293365485	0.0
25	3.5254342453631726E-4	0.0	0.0	19.26438289036252	0.0
26	3.5254342453631726E-4	0.0	0.0	19.479434379329675	0.0
27	3.5254342453631726E-4	0.0	0.0	19.73044529759953	0.0
28	3.5254342453631726E-4	0.0	0.0	19.937035744377813	0.0
29	3.5254342453631726E-4	0.0	0.0	20.166541513750957	0.0
30	3.5254342453631726E-4	0.0	0.0	20.40944393325648	0.0
31	3.5254342453631726E-4	0.0	0.0	20.6363056269456	0.0
32	3.5254342453631726E-4	0.0	0.0	20.877092785903905	0.0
33	3.5254342453631726E-4	0.0	0.0	21.10060531705993	0.0
34	3.5254342453631726E-4	0.0	0.0	21.34456536683906	0.0
35	3.5254342453631726E-4	0.0	0.0	21.60192206675057	0.0
36	3.5254342453631726E-4	0.0	0.0	21.834953270369077	0.0
37	3.5254342453631726E-4	0.0	0.0	22.054059008718397	0.0
38	3.5254342453631726E-4	0.0	0.0	22.31441232773847	0.0
39	3.5254342453631726E-4	0.0	0.0	22.683172749803457	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	20	7.030262E-4	45.000004	6
CGGGATA	55	1.8189894E-12	45.0	6
TCGTTGA	25	3.8883E-5	44.999996	24
GGCATCG	25	3.8883E-5	44.999996	23
GCATCGG	25	3.8883E-5	44.999996	24
GTACGGG	30	2.1635515E-6	44.999996	3
CGTTTTT	20845	0.0	44.73015	1
GACCCGT	115	0.0	43.04348	38
CACCGGT	220	0.0	42.954544	16
GATACCT	1915	0.0	42.29765	5
CGATGAA	215	0.0	41.860466	19
TGATACC	1930	0.0	41.852333	4
TACGGCT	1835	0.0	41.81199	7
ACGGCTG	1835	0.0	41.689373	8
GAATGAT	6120	0.0	41.397057	1
CCGATGA	240	0.0	41.249996	18
CGACCAC	1335	0.0	40.78652	12
AATGATA	5900	0.0	40.76695	2
ATGATAC	6010	0.0	40.54493	3
GCCGATT	50	1.0786607E-9	40.499996	9
>>END_MODULE