##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933473.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 515711 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.56049027459178 31.0 28.0 31.0 16.0 33.0 2 28.74984245051977 31.0 30.0 33.0 16.0 34.0 3 28.905348150417577 31.0 30.0 33.0 16.0 34.0 4 32.14388872837694 35.0 30.0 37.0 19.0 37.0 5 33.72166193856637 35.0 33.0 37.0 30.0 37.0 6 32.723645607714396 35.0 33.0 37.0 17.0 37.0 7 32.31789510016269 35.0 32.0 35.0 25.0 37.0 8 32.9604991943162 35.0 32.0 36.0 27.0 37.0 9 33.14384412975485 35.0 32.0 37.0 25.0 39.0 10 32.48870394465117 35.0 30.0 39.0 17.0 39.0 11 33.19928603423235 37.0 31.0 39.0 17.0 39.0 12 33.84565774241775 37.0 31.0 39.0 26.0 39.0 13 34.035459782707754 37.0 32.0 39.0 27.0 39.0 14 34.029328441704756 37.0 32.0 40.0 22.0 41.0 15 34.63878606428794 37.0 32.0 40.0 26.0 41.0 16 34.82252657011388 37.0 32.0 40.0 26.0 41.0 17 32.84039316593984 36.0 30.0 40.0 18.0 41.0 18 33.441245193528935 36.0 31.0 39.0 24.0 39.0 19 33.21749002832982 36.0 32.0 37.0 23.0 39.0 20 32.63171815222091 35.0 31.0 36.0 25.0 39.0 21 32.958370094878724 35.0 32.0 37.0 25.0 39.0 22 33.07657001692809 35.0 31.0 37.0 25.0 39.0 23 34.03568859302982 35.0 33.0 38.0 27.0 40.0 24 32.30731165323214 35.0 31.0 37.0 21.0 39.0 25 31.302345693615223 34.0 29.0 37.0 18.0 39.0 26 30.544331999899168 33.0 25.0 37.0 19.0 39.0 27 32.4752700640475 34.0 30.0 37.0 22.0 39.0 28 32.47371299041517 35.0 31.0 37.0 21.0 39.0 29 32.51797615331067 35.0 31.0 38.0 21.0 40.0 30 31.456443628311206 35.0 29.0 37.0 18.0 40.0 31 31.807698497802065 35.0 30.0 37.0 20.0 40.0 32 31.574854909047897 35.0 30.0 37.0 17.0 40.0 33 31.157658068181597 35.0 30.0 38.0 14.0 40.0 34 29.892616213344297 35.0 24.0 38.0 8.0 40.0 35 29.36717851664983 35.0 23.0 38.0 7.0 40.0 36 28.52024292675549 34.0 20.0 38.0 7.0 40.0 37 28.68002233809246 35.0 20.0 38.0 7.0 40.0 38 28.549964999777007 34.0 20.0 38.0 7.0 40.0 39 28.32247712381547 34.0 18.0 38.0 7.0 40.0 40 28.014560480579238 33.0 18.0 38.0 7.0 40.0 41 27.79180587577151 33.0 18.0 38.0 7.0 40.0 42 28.03451933350268 33.0 18.0 38.0 7.0 40.0 43 28.074466125407447 34.0 18.0 38.0 7.0 40.0 44 28.030113765267757 34.0 18.0 38.0 7.0 40.0 45 28.22470530975682 34.0 18.0 38.0 7.0 40.0 46 27.98298853427598 34.0 18.0 38.0 7.0 40.0 47 27.995790277888197 34.0 17.0 38.0 7.0 40.0 48 27.723843392908044 34.0 17.0 38.0 7.0 40.0 49 27.421080799129744 33.0 15.0 38.0 7.0 40.0 50 27.592130088363444 34.0 15.0 38.0 7.0 40.0 51 26.12080603283622 31.0 14.0 36.0 7.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 3.0 10 10.0 11 15.0 12 30.0 13 67.0 14 121.0 15 253.0 16 555.0 17 1094.0 18 1911.0 19 3085.0 20 4869.0 21 7066.0 22 9992.0 23 14010.0 24 18996.0 25 25714.0 26 30624.0 27 30003.0 28 27907.0 29 27009.0 30 29195.0 31 33298.0 32 38380.0 33 43778.0 34 48842.0 35 51182.0 36 40271.0 37 22417.0 38 4917.0 39 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.03363705641338 2.1971608129359272 32.09510753115602 42.67409459949468 2 43.70238369939753 12.308444070419284 31.226016121432355 12.763156108750831 3 11.585752485403647 12.33365198725643 63.25597088291698 12.824624644422942 4 10.339899672490988 2.5847810110701537 71.599985263064 15.475334053374857 5 18.415740598901323 3.0726511553951728 62.71729709081249 15.794311154891014 6 13.095900611001124 10.918518317429724 65.01819817688589 10.967382894683263 7 49.801342224618054 0.932499015921708 46.86248693551233 2.4036718239479087 8 50.4854463061676 7.964344371169124 38.402516137914446 3.1476931847488223 9 46.491348836848545 2.723424553674442 38.14888571312227 12.636340896354742 10 22.735020195419526 21.158555857835104 44.17648644298842 11.92993750375695 11 18.386654541012312 16.330076341206606 53.01826022714272 12.265008890638361 12 14.8825601935968 15.071425662822783 54.20031761975215 15.845696523828268 13 14.802476580875723 15.851707642458665 58.60123208541218 10.744583691253434 14 13.68945009898955 18.587154433393895 52.48210722672194 15.24128824089461 15 11.344919926082632 16.394841296772807 58.60685538993739 13.653383387207176 16 12.715842787918039 16.531739675903754 51.47223929681547 19.280178239362744 17 12.930497895138945 16.782849309012217 55.792100614491446 14.494552181357387 18 12.926425847034482 15.789851292681368 54.30114928710073 16.98257357318343 19 12.994681129547365 18.723471091367063 51.49085437386444 16.79099340522114 20 15.248850615945752 17.8543796816434 55.62727961978705 11.269490082623795 21 15.942650050124973 20.410268541877137 51.39991196619812 12.247169441799768 22 13.975850815670016 15.372757222552941 53.78225401436075 16.869137947416284 23 13.273519471176687 20.972211180292838 52.40066626463271 13.353603083897763 24 13.740253746769024 17.285844203439524 52.30448836654637 16.669413683245075 25 12.275867685583593 22.515711318936383 50.199045589487135 15.009375405992891 26 12.934569943243407 18.450062147210357 53.083606903866695 15.531761005679536 27 14.214550397412504 19.144831116652544 50.547302655944904 16.09331582999005 28 11.40503111238659 19.138626090969556 54.83400586762741 14.622336929016445 29 15.498990713791253 18.055267388130176 50.93918880923618 15.506553088842395 30 14.937241982428143 20.31893832010564 51.828058738324366 12.915760959141846 31 15.80090399467919 18.06069678560279 50.349711369352214 15.788687850365807 32 17.344016319217545 21.447865180304472 47.99936398486749 13.208754515610488 33 15.113697400288146 20.737583646654812 45.5483788400868 18.600340112970247 34 17.901886909528784 23.90815786360966 43.96881198966088 14.221143237200682 35 17.697508876095334 22.609368425339 42.94207414617877 16.751048552386898 36 18.009311416665536 23.67294860881385 42.085780601926274 16.23195937259434 37 20.968720853346156 22.755768250047023 41.08560802464947 15.189902871957356 38 17.4041275055215 25.10301312169025 41.445887328368016 16.046972044420226 39 23.08696149587657 22.995631274105072 38.41453837517525 15.502868854843118 40 18.302692787239362 24.765033129019937 41.82555733734591 15.106716746394783 41 22.625074896599067 23.894196555822933 37.95594819579183 15.524780351786175 42 17.921083707735534 23.089676194612874 42.78733631820923 16.20190377944236 43 21.001491145234443 23.078235678509863 37.572400045762066 18.347873130493632 44 20.59855228994534 23.09626903440105 37.77891105677404 18.526267618879565 45 17.211577802296247 22.345848740864554 39.48354795612271 20.959025500716486 46 21.38852962221089 24.264752933328936 36.821010216962605 17.525707227497573 47 16.556753685688303 23.04527147956898 43.01944306016354 17.378531774579173 48 19.887495128085302 23.108097364609247 37.010263500293775 19.99414400701168 49 16.88251753404523 22.09590255007165 41.063502620653814 19.958077295229305 50 19.05001056793437 21.497893199873573 38.988115436746554 20.46398079544551 51 18.58637880518352 21.609583662167374 37.37170624632788 22.432331286321215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64.0 1 388.5 2 713.0 3 20630.5 4 40548.0 5 29148.0 6 17748.0 7 16967.0 8 16186.0 9 15987.0 10 15788.0 11 15236.5 12 14685.0 13 14141.5 14 13598.0 15 12855.0 16 12112.0 17 11429.5 18 10747.0 19 10020.0 20 9293.0 21 8655.0 22 8017.0 23 7598.5 24 7180.0 25 7021.5 26 7104.0 27 7345.0 28 7514.5 29 7684.0 30 8483.5 31 9283.0 32 10038.5 33 10794.0 34 12027.0 35 13260.0 36 14160.5 37 15061.0 38 16193.0 39 17325.0 40 17996.0 41 18667.0 42 19661.0 43 20655.0 44 22363.5 45 24072.0 46 40684.5 47 57297.0 48 46726.5 49 36156.0 50 32582.0 51 29008.0 52 25017.0 53 21026.0 54 18200.5 55 15375.0 56 13653.0 57 11931.0 58 10329.5 59 8728.0 60 7415.0 61 6102.0 62 5237.5 63 4373.0 64 3523.0 65 2673.0 66 2265.5 67 1858.0 68 1504.5 69 1151.0 70 919.0 71 687.0 72 592.0 73 497.0 74 498.0 75 423.0 76 347.0 77 252.0 78 157.0 79 119.5 80 82.0 81 61.5 82 41.0 83 28.0 84 15.0 85 12.5 86 10.0 87 8.0 88 6.0 89 4.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 515711.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.66033901614354 #Duplication Level Percentage of deduplicated Percentage of total 1 82.73140735141466 41.91201143705337 2 6.613556251661235 6.700900036229875 3 2.9515538407809614 4.485801545950921 4 1.8677948781552327 3.7849248695984246 5 1.3522674902918794 3.4253164749348106 6 1.0352056902006397 3.146632273020317 7 0.7466232291665994 2.647693013483543 8 0.5889745516441297 2.38701203665382 9 0.4635019591485019 2.1133049746598838 >10 1.5634252174786374 11.32555238058895 >50 0.04089728048918279 1.487800048621142 >100 0.038170795110407406 3.7745526688423707 >500 0.0031159832743189717 1.0675064006710873 >1k 0.0019474895464493575 1.6788067388002976 >5k 7.789958185797429E-4 2.826425449762043 >10k+ 7.789958185797429E-4 7.235759651129163 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26093 5.059616723319844 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG 10577 2.0509548952804963 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC 7683 1.489787885075168 No Hit GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC 6641 1.287736736272835 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT 2249 0.43609696128257885 No Hit GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT 2028 0.39324350265943525 No Hit GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC 1816 0.35213520750963234 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC 1268 0.2458741426884437 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTA 1147 0.2224113893246411 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT 871 0.16889304280886 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCAGTGAGTGTCGTATGCCG 839 0.1626880171258709 No Hit GAATCTTTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC 813 0.15764643375844223 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT 775 0.15027796575989266 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT 538 0.10432199429525452 No Hit GAATGCTGCCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT 535 0.10374027313747429 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC 523 0.10141338850635337 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 516 0.10005603913819949 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.10141338850635337 0.0 2 0.0 0.0 0.0 0.6369846677693515 0.0 3 0.0 0.0 0.0 0.8182877619441896 0.0 4 0.0 0.0 0.0 1.4942477472848166 0.0 5 0.0 0.0 0.0 3.198496832528296 0.0 6 0.0 0.0 0.0 3.6553418484383697 0.0 7 0.0 0.0 0.0 4.07166029035642 0.0 8 0.0 0.0 0.0 4.601608265094209 0.0 9 0.0 0.0 0.0 4.809088811369159 0.0 10 0.0 0.0 0.0 7.42334369443351 0.0 11 0.0 0.0 0.0 7.905784441285914 0.0 12 0.0 0.0 0.0 9.992612141296192 0.0 13 0.0 0.0 0.0 10.230536094828304 0.0 14 0.0 0.0 0.0 10.392060669638616 0.0 15 0.0 0.0 0.0 10.807215669241106 0.0 16 0.0 0.0 0.0 11.079461171082253 0.0 17 0.0 0.0 0.0 11.337939272189269 0.0 18 0.0 0.0 0.0 11.58594639245624 0.0 19 0.0 0.0 0.0 12.323374913468978 0.0 20 0.0 0.0 0.0 12.576035802998192 0.0 21 0.0 0.0 0.0 12.825400272633315 0.0 22 0.0 0.0 0.0 13.152133656253211 0.0 23 0.0 0.0 0.0 13.43349278956625 0.0 24 0.0 0.0 0.0 13.68595977204287 0.0 25 0.0 0.0 0.0 13.88393887274074 0.0 26 0.0 0.0 0.0 14.067374944494107 0.0 27 0.0 0.0 0.0 14.266711394560131 0.0 28 0.0 0.0 0.0 14.437543507894926 0.0 29 0.0 0.0 0.0 14.64366670480172 0.0 30 0.0 0.0 0.0 14.852504600444822 0.0 31 0.0 0.0 0.0 15.045829931880453 0.0 32 0.0 0.0 0.0 15.250595779419093 0.0 33 1.9390705259340988E-4 0.0 0.0 15.45691288337848 0.0 34 1.9390705259340988E-4 0.0 0.0 15.66322998733787 0.0 35 1.9390705259340988E-4 0.0 0.0 15.8742008605595 0.0 36 1.9390705259340988E-4 0.0 0.0 16.088274246622625 0.0 37 1.9390705259340988E-4 0.0 0.0 16.282763020373814 0.0 38 1.9390705259340988E-4 0.0 0.0 16.47085286138942 0.0 39 1.9390705259340988E-4 0.0 0.0 16.68182373461105 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTG 90 0.0 45.000004 1 ATCCGTC 30 2.163164E-6 45.000004 6 CACCCGT 30 2.163164E-6 45.000004 16 TGGCGAT 50 2.1827873E-11 45.0 6 CGACCGG 20 7.0296664E-4 45.0 2 CCGCTTG 20 7.0296664E-4 45.0 1 CGTTCGG 20 7.0296664E-4 45.0 2 CCCGTTG 25 3.887804E-5 45.0 1 CGGTGTA 25 3.887804E-5 45.0 19 ACCCGTC 20 7.0296664E-4 45.0 17 TGCACGG 25 3.887804E-5 45.0 2 CGTTTTT 16710 0.0 44.394073 1 CGATGAA 165 0.0 43.636368 19 CCGATGA 195 0.0 42.69231 18 TGATCCG 95 0.0 42.63158 4 ACGGCTG 985 0.0 42.48731 8 CGGCTGT 935 0.0 42.1123 9 GATACCT 1400 0.0 41.625 5 CCGGGAG 125 0.0 41.399998 5 CGACCAC 930 0.0 41.370968 12 >>END_MODULE