##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933472.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209565
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.974957650371007	31.0	28.0	31.0	25.0	34.0
2	27.64407224488822	31.0	25.0	33.0	16.0	34.0
3	27.60845083864195	31.0	25.0	33.0	16.0	34.0
4	32.70923579796245	35.0	32.0	37.0	28.0	37.0
5	34.105924176269895	35.0	33.0	37.0	30.0	37.0
6	33.02363944360938	35.0	32.0	37.0	28.0	37.0
7	33.65775296447403	35.0	33.0	37.0	29.0	37.0
8	33.03314484766063	35.0	32.0	37.0	27.0	37.0
9	34.7165223200439	37.0	34.0	39.0	28.0	39.0
10	34.66305442225563	37.0	33.0	39.0	28.0	39.0
11	35.000391286712954	37.0	34.0	39.0	28.0	39.0
12	34.99012716818171	37.0	34.0	39.0	27.0	39.0
13	34.92955884809009	37.0	34.0	39.0	27.0	39.0
14	35.71837854603584	38.0	34.0	40.0	27.0	41.0
15	35.90750363848925	38.0	34.0	40.0	27.0	41.0
16	36.12486817932384	38.0	34.0	40.0	30.0	41.0
17	35.259017488607356	37.0	33.0	40.0	27.0	41.0
18	35.09308806336936	37.0	33.0	39.0	27.0	40.0
19	34.50899720850333	37.0	33.0	37.0	27.0	40.0
20	33.23740128361129	35.0	32.0	37.0	25.0	39.0
21	34.0180373631093	35.0	33.0	38.0	27.0	40.0
22	34.52942523799299	35.0	33.0	38.0	28.0	40.0
23	34.66663326414239	35.0	33.0	39.0	29.0	40.0
24	34.36526614654164	35.0	33.0	38.0	27.0	40.0
25	33.378073628707085	35.0	32.0	38.0	24.0	40.0
26	33.37011428435092	35.0	32.0	38.0	24.0	40.0
27	34.045971416982795	35.0	33.0	38.0	25.0	40.0
28	33.59599169708682	35.0	33.0	38.0	24.0	40.0
29	33.50319948464677	35.0	33.0	38.0	23.0	40.0
30	32.83414692338892	35.0	31.0	38.0	21.0	40.0
31	32.975945410731754	35.0	32.0	38.0	21.0	40.0
32	32.27470713143893	35.0	31.0	38.0	16.0	40.0
33	31.4795838999833	35.0	30.0	39.0	12.0	40.0
34	30.966554529620883	35.0	28.0	39.0	9.0	40.0
35	30.337680433278457	35.0	24.0	39.0	8.0	40.0
36	29.772547896833917	35.0	22.0	39.0	7.0	40.0
37	29.449497769188557	35.0	21.0	39.0	7.0	40.0
38	29.24115190990862	35.0	21.0	39.0	7.0	40.0
39	28.95594684226851	35.0	20.0	38.0	7.0	40.0
40	28.776942714670867	35.0	20.0	38.0	7.0	40.0
41	28.296762341039774	34.0	18.0	38.0	7.0	40.0
42	28.594631737169852	35.0	18.0	38.0	7.0	40.0
43	28.443714360699545	35.0	18.0	39.0	7.0	40.0
44	28.601569918640994	35.0	18.0	39.0	7.0	40.0
45	28.655729725860713	35.0	18.0	39.0	7.0	40.0
46	28.39211223248157	35.0	18.0	39.0	7.0	40.0
47	28.254780139813423	34.0	18.0	38.0	7.0	40.0
48	28.004485481831413	34.0	18.0	38.0	7.0	40.0
49	28.223448572042088	34.0	17.0	39.0	7.0	40.0
50	28.143917161739793	34.0	18.0	39.0	7.0	40.0
51	27.06681459213132	33.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	16.0
11	12.0
12	11.0
13	21.0
14	37.0
15	65.0
16	118.0
17	249.0
18	528.0
19	899.0
20	1543.0
21	2256.0
22	3158.0
23	4455.0
24	6289.0
25	8941.0
26	11617.0
27	12388.0
28	11060.0
29	9897.0
30	10047.0
31	11082.0
32	13002.0
33	15177.0
34	17179.0
35	18561.0
36	18952.0
37	17386.0
38	12688.0
39	1917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.10111421277408	2.216973254121633	25.58108462768115	38.10082790542314
2	36.94032877627467	25.8945911769618	26.724882494691382	10.44019755207215
3	9.564097058191969	26.30735094123542	53.07279364397681	11.055758356595806
4	9.337914250948392	2.3925750960322576	75.65624030730322	12.613270345716126
5	27.158160952449123	2.78481616682175	55.00059647364779	15.056426407081336
6	10.512251568725693	22.122491828311023	58.64958366139385	8.71567294156944
7	42.73805263283468	1.0163910958413858	53.45739985207454	2.7881564192493977
8	40.19182592513062	18.01970748932312	37.5434829289242	4.2449836566220505
9	38.027342352014884	2.425023262472264	35.71159306181853	23.836041323694317
10	29.528785818242547	14.942857824541312	42.29093598644812	13.23742037076802
11	25.804404361415312	13.17300121680624	48.10631546298285	12.916278958795601
12	18.777467611480926	11.681339918402404	48.6207143368406	20.92047813327607
13	17.06200940042469	14.681840956266552	58.86049674325389	9.395652900054875
14	14.42845895068356	23.480065850690718	45.255171426526374	16.83630377209935
15	8.4589506835588	14.14262877866056	62.769546441438216	14.628874096342424
16	10.378164292701548	15.84949776918856	47.4626011022833	26.30973683582659
17	10.906401355188128	17.411304368572996	55.430534678977885	16.251759597260993
18	10.233579080476224	16.330016939851596	52.55028272850906	20.88612125116312
19	11.780593133395366	19.55336053253167	48.11729057810226	20.548755755970703
20	14.637940495788895	17.342590604347098	56.68837830744637	11.331090592417628
21	14.251902750936463	25.961396225514754	47.93405387349987	11.85264715004891
22	10.852480137427529	13.516570037935724	53.11907999904564	22.511869825591106
23	12.420967241667263	24.817598358504522	49.764035024932596	12.997399374895618
24	15.363729630424928	16.295660057738647	47.145754300575	21.19485601126142
25	10.434471405053324	29.694366902870232	44.998449168515734	14.872712523560708
26	12.726361749337913	14.990098537446617	52.567938348483764	19.715601364731704
27	16.24889652375158	20.004294610264118	45.57679001741703	18.17001884856727
28	11.63123613198769	18.549853267482643	53.914537255744044	15.904373344785627
29	20.47002123446186	15.206260587407247	47.52511154057214	16.798606637558752
30	13.027461646744449	23.39178775081717	52.01870541359482	11.562045188843557
31	22.13012669100279	14.967193949371316	42.579152053062295	20.323527306563598
32	20.340228568701836	26.497745329611337	41.17004270751318	11.991983394173646
33	15.661966454322048	19.609190465965217	40.930498890559015	23.798344189153724
34	28.869801732159473	19.051845489466277	37.47667788037125	14.601674898003006
35	13.72939183546871	20.069668122062367	43.10691193663064	23.094028105838284
36	26.420442344857204	18.966907642020374	38.346575048314364	16.266074964808055
37	14.962422160188963	24.991291484742202	45.22892658602343	14.817359769045405
38	21.145229403764944	25.66888554863646	34.22231765800587	18.96356738959273
39	24.22303342638322	25.032806050628682	35.67580464295087	15.06835588003722
40	15.583231932813208	23.864194879870208	38.48495693460263	22.067616252713957
41	21.430582396869706	22.677928089137023	38.939231264762725	16.95225824923055
42	15.475866676210245	21.139980435664352	41.53985636914561	21.844296518979792
43	20.39892157564479	20.58931596402071	37.67613866819364	21.33562379214086
44	20.666618948774843	20.69286378927779	38.35755016343378	20.282967098513588
45	16.958938754085846	20.285830172023	36.30424927826689	26.45098179562427
46	23.785937537279604	26.155608045236562	31.93758499749481	18.120869419989024
47	14.030014553957008	21.10180612220552	46.087848638847134	18.780330684990336
48	21.95023023882805	23.042492782668862	31.92517834562069	23.0820986328824
49	15.692983084007347	18.80180373631093	42.05425524300336	23.450957936678357
50	19.18593276549042	18.313649702956123	38.45298594708086	24.047431584472598
51	19.039916016510393	18.8027580941474	34.71906091188891	27.438264977453297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	270.5
2	512.0
3	6411.0
4	12310.0
5	9546.5
6	6783.0
7	6892.5
8	7002.0
9	6983.5
10	6965.0
11	6572.0
12	6179.0
13	5797.0
14	5415.0
15	4987.0
16	4559.0
17	4115.5
18	3672.0
19	3410.5
20	3149.0
21	2918.0
22	2687.0
23	2593.0
24	2499.0
25	2363.0
26	2377.0
27	2527.0
28	2550.0
29	2573.0
30	2592.5
31	2612.0
32	2731.5
33	2851.0
34	3112.0
35	3373.0
36	3536.5
37	3700.0
38	3905.0
39	4110.0
40	4727.0
41	5344.0
42	5878.0
43	6412.0
44	7220.0
45	8028.0
46	26380.5
47	44733.0
48	29762.5
49	14792.0
50	13191.5
51	11591.0
52	9413.5
53	7236.0
54	6473.0
55	5710.0
56	5208.0
57	4706.0
58	4557.5
59	4409.0
60	3926.0
61	3443.0
62	2954.0
63	2465.0
64	2094.0
65	1723.0
66	1436.5
67	1150.0
68	922.5
69	695.0
70	629.0
71	563.0
72	452.0
73	341.0
74	267.5
75	160.0
76	126.0
77	90.0
78	54.0
79	66.5
80	79.0
81	45.5
82	12.0
83	14.5
84	17.0
85	11.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	209565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.23765896022714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.85590049773207	37.55445804404361
2	4.885720276787586	4.224942142056164
3	1.609076160731037	2.087180588361606
4	1.0009822207016807	1.7312051153580037
5	0.6798291598150334	1.4697110681650085
6	0.5562238580304819	1.4429890487438264
7	0.5198044387546766	1.5732588934220888
8	0.43703303130966437	1.511702812969723
9	0.43924026884153133	1.7092548851191756
>10	2.9135535420644296	21.427242144442058
>50	0.04966284446700732	1.4792546465297163
>100	0.03973027557360585	2.69367499343879
>500	0.0022072375318669918	0.5458926824612889
>1k	0.006621712595600977	4.926395151862191
>5k	0.0044144750637339835	15.622837783026746
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	9994	4.768926108844511	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	8742	4.1714981032138	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	7731	3.6890702168778184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6273	2.9933433540906162	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	2082	0.9934865077660868	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	1784	0.8512871901319399	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	1683	0.8030921193901653	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	1628	0.7768472788872187	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	1593	0.7601460167489801	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	1554	0.7415360389377997	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGT	622	0.29680528714241405	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	522	0.24908739531887483	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTAT	461	0.21997948130651587	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATG	390	0.186099778111803	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTGAAGCTT	282	0.13456445494238065	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGAAGCT	258	0.12311216090473123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10402500417531553	0.0
2	0.0	0.0	0.0	1.0493164411996279	0.0
3	0.0	0.0	0.0	1.1690883496767113	0.0
4	0.0	0.0	0.0	2.1253549018204376	0.0
5	0.0	0.0	0.0	6.45193615346074	0.0
6	0.0	0.0	0.0	6.81029752105552	0.0
7	0.0	0.0	0.0	7.748908453224536	0.0
8	0.0	0.0	0.0	8.843556891656526	0.0
9	0.0	0.0	0.0	8.991005177391262	0.0
10	0.0	0.0	0.0	14.842650251711879	0.0
11	0.0	0.0	0.0	15.109870445923699	0.0
12	0.0	0.0	0.0	20.214730513205925	0.0
13	0.0	0.0	0.0	20.57547777539188	0.0
14	0.0	0.0	0.0	20.885644072244887	0.0
15	0.0	0.0	0.0	21.894877484312744	0.0
16	0.0	0.0	0.0	22.33960823610813	0.0
17	0.0	0.0	0.0	22.557679001741704	0.0
18	0.0	0.0	0.0	22.70608164531291	0.0
19	0.0	0.0	0.0	24.07319924605731	0.0
20	0.0	0.0	0.0	24.33517047216854	0.0
21	0.0	0.0	0.0	24.476415431966217	0.0
22	0.0	0.0	0.0	24.85290959845394	0.0
23	0.0	0.0	0.0	25.0141960728175	0.0
24	0.0	0.0	0.0	25.14398873857753	0.0
25	0.0	0.0	0.0	25.245627848161668	0.0
26	0.0	0.0	0.0	25.415980721971703	0.0
27	0.0	0.0	0.0	25.63596020327822	0.0
28	0.0	0.0	0.0	25.72805573449765	0.0
29	0.0	0.0	0.0	25.844487390547087	0.0
30	0.0	0.0	0.0	25.959487509841814	0.0
31	0.0	0.0	0.0	26.07591916589125	0.0
32	0.0	0.0	0.0	26.202848758141865	0.0
33	0.0	0.0	0.0	26.305442225562473	0.0
34	0.0	0.0	0.0	26.50490301338487	0.0
35	0.0	0.0	0.0	26.61656288025195	0.0
36	0.0	0.0	0.0	26.747309903848446	0.0
37	0.0	0.0	0.0	26.89523536850142	0.0
38	0.0	0.0	0.0	27.021210602915563	0.0
39	0.0	0.0	0.0	27.181542719442653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATTG	20	7.020073E-4	45.000004	35
GCAAGTG	20	7.020073E-4	45.000004	9
TTTCGTG	30	2.156983E-6	45.000004	1
AGCCAAC	20	7.020073E-4	45.000004	35
GATCCGG	20	7.020073E-4	45.000004	5
ACCGACG	20	7.020073E-4	45.000004	17
TTGTACT	20	7.020073E-4	45.000004	1
CCTGTTG	20	7.020073E-4	45.000004	1
GACCCGT	30	2.156983E-6	45.000004	38
ATCAAAT	20	7.020073E-4	45.000004	43
ACGGCGG	20	7.020073E-4	45.000004	30
GATAGCC	20	7.020073E-4	45.000004	9
TGACTCT	20	7.020073E-4	45.000004	4
TCCGGGC	20	7.020073E-4	45.000004	4
TGATCCG	25	3.8798586E-5	45.0	4
AGTTGGG	50	2.1827873E-11	45.0	3
CGGGATA	35	1.2055716E-7	45.0	6
GTCCGGG	25	3.8798586E-5	45.0	3
GCGATTT	45	3.8198777E-10	45.0	9
CATCCGC	25	3.8798586E-5	45.0	42
>>END_MODULE