SRR2933471.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138626
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9004	6.495174065471124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	4990	3.5996133481453696	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	3734	2.693578405205373	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	2562	1.8481381559014902	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	996	0.718479938828214	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	852	0.6146033211663036	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	747	0.5388599541211605	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	694	0.500627587898374	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	587	0.4234414900523711	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	445	0.32100760319132055	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGAAGCTTCGTATGCCG	429	0.3094657567844416	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	247	0.17817725390619363	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	242	0.17457042690404398	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	233	0.16807813830017457	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGT	212	0.15292946489114595	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGTGAAGCTTCGTATGC	192	0.13850215688254727	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTAT	182	0.13128850287824795	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	178	0.1284030412765282	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	178	0.1284030412765282	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	177	0.12768167587609827	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	167	0.12046802187179893	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	158	0.11397573326792952	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	156	0.11253300246706965	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	139	0.1002697906597608	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	139	0.1002697906597608	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	45	3.8016879E-10	45.000004	17
CTCGTAA	35	1.2016244E-7	45.000004	33
ATGACTC	45	3.8016879E-10	45.000004	3
TCGTAAC	35	1.2016244E-7	45.000004	34
CGTAACC	35	1.2016244E-7	45.000004	35
CCGGTGT	35	1.2016244E-7	45.000004	18
CACCGGT	70	0.0	45.000004	16
GATAAAA	40	6.7411747E-9	45.0	19
ACTGACG	20	7.011811E-4	45.0	28
ACCGCTG	25	3.8730162E-5	45.0	17
TCCCTTG	25	3.8730162E-5	45.0	1
CCGTGTC	20	7.011811E-4	45.0	19
GGATCCT	25	3.8730162E-5	45.0	8
GAATAGC	40	6.7411747E-9	45.0	27
CGCCTCT	20	7.011811E-4	45.0	16
AACATGC	40	6.7411747E-9	45.0	43
GGGGTAT	25	3.8730162E-5	45.0	7
GCGGGAT	20	7.011811E-4	45.0	5
CTGTTGG	55	1.8189894E-12	45.0	2
CGAATAG	40	6.7411747E-9	45.0	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content