##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138626
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.767922323373682	31.0	25.0	31.0	16.0	33.0
2	27.554376523884407	31.0	25.0	33.0	16.0	34.0
3	27.66093662083592	31.0	25.0	33.0	16.0	34.0
4	31.26313245711483	35.0	28.0	35.0	19.0	37.0
5	33.5421060984231	35.0	33.0	37.0	28.0	37.0
6	31.660929407181914	35.0	32.0	37.0	17.0	37.0
7	32.362861223724266	35.0	32.0	35.0	26.0	37.0
8	32.17396447996768	35.0	32.0	36.0	17.0	37.0
9	33.155180124940486	35.0	32.0	37.0	26.0	39.0
10	32.0271810482882	35.0	28.0	39.0	17.0	39.0
11	32.67506095537634	35.0	30.0	39.0	17.0	39.0
12	33.39665719273441	35.0	31.0	39.0	23.0	39.0
13	33.41034149438056	35.0	31.0	39.0	25.0	39.0
14	33.47672875218213	36.0	31.0	40.0	19.0	41.0
15	34.01923160157546	36.0	32.0	40.0	23.0	41.0
16	34.29817638826771	36.0	32.0	40.0	25.0	41.0
17	32.228110166923955	35.0	27.0	39.0	18.0	41.0
18	32.91065889515675	36.0	30.0	39.0	23.0	39.0
19	32.74095768470561	36.0	30.0	37.0	21.0	39.0
20	31.897126080244686	34.0	30.0	35.0	23.0	39.0
21	32.47140507552696	35.0	31.0	37.0	23.0	39.0
22	32.72448891261379	34.0	30.0	37.0	25.0	39.0
23	33.51227042546131	35.0	32.0	38.0	26.0	39.0
24	32.18950269069294	35.0	31.0	37.0	21.0	39.0
25	31.313613607836913	34.0	29.0	37.0	18.0	39.0
26	30.460115707010228	33.0	25.0	37.0	18.0	39.0
27	32.2676265635595	34.0	30.0	37.0	22.0	39.0
28	32.12227143537287	35.0	30.0	37.0	21.0	39.0
29	32.123728593481744	35.0	30.0	37.0	21.0	39.0
30	31.135681618166867	34.0	28.0	37.0	18.0	39.0
31	31.62812170877036	34.0	30.0	37.0	20.0	39.0
32	31.400372224546622	35.0	30.0	37.0	18.0	39.0
33	31.201737047884237	35.0	30.0	37.0	15.0	40.0
34	30.337808203367334	35.0	27.0	38.0	10.0	40.0
35	29.73479722418594	35.0	24.0	38.0	8.0	40.0
36	29.045482088497106	34.0	22.0	38.0	7.0	40.0
37	28.65155887063033	34.0	21.0	37.0	7.0	40.0
38	28.585366381486878	34.0	21.0	37.0	7.0	40.0
39	28.120489662833812	33.0	20.0	37.0	7.0	39.0
40	27.956047206151805	33.0	20.0	37.0	7.0	39.0
41	27.40394298327875	33.0	18.0	37.0	7.0	39.0
42	28.085633286685038	33.0	20.0	37.0	7.0	40.0
43	27.893699594592643	33.0	18.0	37.0	7.0	40.0
44	27.788748142484096	33.0	18.0	37.0	7.0	40.0
45	27.975091252723153	34.0	18.0	38.0	7.0	40.0
46	27.55068313303421	33.0	17.0	37.0	7.0	40.0
47	27.620576226681862	33.0	18.0	37.0	7.0	40.0
48	27.358864859405884	33.0	17.0	37.0	7.0	40.0
49	27.33863777357783	33.0	17.0	37.0	7.0	40.0
50	27.3951351117395	33.0	17.0	37.0	7.0	40.0
51	25.85417598430309	31.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	8.0
12	6.0
13	17.0
14	44.0
15	82.0
16	204.0
17	346.0
18	575.0
19	950.0
20	1400.0
21	1993.0
22	2697.0
23	3645.0
24	4855.0
25	6432.0
26	8419.0
27	9078.0
28	8509.0
29	8560.0
30	9097.0
31	10195.0
32	11297.0
33	12010.0
34	12555.0
35	12490.0
36	8218.0
37	4068.0
38	850.0
39	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.595645838443005	2.3213538585835267	28.555249376018928	40.52775092695454
2	41.35299294504639	19.71203093214837	28.053900422720123	10.881075700085121
3	10.068096893800586	19.862074935437796	58.63113701614415	11.43869115461746
4	10.050062758789839	2.3956544948278102	74.23066380044148	13.323618945940877
5	23.17747031581377	2.751287637239767	59.83437450406128	14.236867542885173
6	10.950326778526396	16.844603465439384	62.064836322190644	10.140233433843578
7	43.13260138790703	1.0207320416083563	52.889068428721885	2.9575981417627286
8	42.53458947095062	13.806212398828501	38.82460721653946	4.834590913681416
9	39.32018524663483	2.409360437435979	38.43651263110816	19.83394168482103
10	26.93650541745416	15.169593005641078	43.76740294028537	14.126498636619395
11	23.31525110729589	13.853101149856448	50.874294865321076	11.957352877526581
12	19.172449612626778	12.602974910911374	50.26185564035607	17.96271983610578
13	16.289152107108336	15.467516916018639	58.960079638740204	9.283251338132818
14	13.358965850561944	20.35332477313058	48.448343023675214	17.839366352632265
15	8.48830666685903	14.757693361995585	62.48322825444001	14.27077171670538
16	10.35087213076912	14.016129730353613	50.37799546982529	25.25500266905198
17	10.652402868148831	16.36201001255176	56.71014095479925	16.275446164500167
18	9.995960353757592	14.893310057276413	55.221964133712284	19.888765455253704
19	11.966009262331742	18.93512039588533	50.09738432905804	19.001486012724886
20	14.015408364953183	16.561828228470848	57.7957958824463	11.626967524129672
21	14.4785249520292	23.4551959949793	49.36591981302209	12.700359239969414
22	11.024627414770679	13.936058170905891	53.66453623418407	21.374778180139366
23	12.117495996422027	23.365025319925557	50.452296106069575	14.06518257758285
24	14.163288272041319	15.26481323849783	50.1348953298804	20.437003159580456
25	10.763493139815042	24.72191363813426	48.03283655302757	16.481756669023127
26	12.333184251150579	15.656514650931284	54.31520782537186	17.695093272546274
27	13.587638682498232	18.92069308787673	49.43156406446121	18.060104165163825
28	11.572143753696999	17.52340830724395	56.01041651638221	14.894031422676843
29	18.152438936418854	15.041189964364548	50.67736211100371	16.129008988212888
30	12.922539783301835	22.181986063220464	53.19059916610159	11.704874987376106
31	19.38236694415189	15.426399088194135	45.98992973901	19.201304228643977
32	18.796618239002786	24.07268477774732	44.79102044349545	12.339676539754448
33	14.997908040338753	18.259201015682482	44.742688961666644	22.00020198231212
34	25.12659962777545	18.701398006146032	40.95119241700691	15.220809949071604
35	13.219020962878536	19.155136843016464	46.255392206368214	21.37044998773679
36	24.481698959791093	17.772279370392276	41.19357119155137	16.55245047826526
37	14.633618513121638	23.806500944988674	46.57495707875867	14.984923463131015
38	19.576414236867542	25.03931441432343	38.25400718479939	17.13026416400964
39	21.584695511664478	23.483329245596064	39.79628641091858	15.13568883182087
40	15.662285574134721	21.62581333948898	41.19573528775266	21.516165798623636
41	19.746656471369008	20.061171785956457	43.05830075166275	17.133870991011786
42	15.272748257902558	19.47037352300434	44.31564064461212	20.94123757448098
43	19.088771226176906	18.932234934283613	41.08248091988516	20.896512919654324
44	20.076320459365487	19.787052933793085	41.03487080345678	19.101755803384645
45	17.157676049225977	19.81518618440985	39.035245913465005	23.991891852899165
46	21.239161484858542	25.125878262375025	35.257455311413445	18.377504941352992
47	14.171944656846481	20.59570354767504	46.137809646098134	19.09454214938035
48	21.3682858915355	21.508230779218906	35.24952029200871	21.873963037236884
49	15.714945248366108	18.20870543765239	42.212139136958434	23.864210177023068
50	18.488595213019202	17.791756236203888	39.494034308138446	24.225614242638464
51	18.64441013951207	18.00239493312943	37.37899095407788	25.974203973280623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	120.0
2	208.0
3	6340.5
4	12473.0
5	8610.0
6	4747.0
7	4843.5
8	4940.0
9	4971.5
10	5003.0
11	4675.0
12	4347.0
13	3889.0
14	3431.0
15	3072.5
16	2714.0
17	2444.5
18	2175.0
19	2022.0
20	1869.0
21	1703.5
22	1538.0
23	1495.5
24	1453.0
25	1357.5
26	1358.5
27	1455.0
28	1488.5
29	1522.0
30	1526.0
31	1530.0
32	1650.0
33	1770.0
34	1810.0
35	1850.0
36	2049.5
37	2249.0
38	2368.5
39	2488.0
40	2975.5
41	3463.0
42	3931.5
43	4400.0
44	5053.5
45	5707.0
46	13485.0
47	21263.0
48	16751.0
49	12239.0
50	10873.5
51	9508.0
52	7731.5
53	5955.0
54	5030.5
55	4106.0
56	3624.0
57	3142.0
58	2978.5
59	2815.0
60	2519.5
61	2224.0
62	1865.0
63	1506.0
64	1298.0
65	1090.0
66	948.5
67	807.0
68	635.0
69	463.0
70	413.5
71	364.0
72	297.0
73	230.0
74	171.5
75	99.5
76	86.0
77	57.5
78	29.0
79	33.5
80	38.0
81	23.5
82	9.0
83	8.0
84	7.0
85	5.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	138626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.08245206526914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.2773937532002	37.99431564064461
2	5.979582693292371	5.391485002813325
3	2.2657450076804917	3.0643602210263587
4	1.387288786482335	2.5016952086910105
5	1.0304659498207887	2.3227965893843865
6	0.7984511008704558	2.1597680088872218
7	0.6336405529953917	1.9996248899917763
8	0.5232334869431644	1.8870918875247067
9	0.4896313364055299	1.9866403127840375
>10	2.4961597542242706	17.798248524807754
>50	0.056003584229390675	1.7514751922438792
>100	0.04800307219662058	3.7099822544111496
>500	0.008000512032770097	2.796012292066423
>1k	0.004800307219662059	8.141329909252233
>5k	0.0016001024065540196	6.495174065471124
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9004	6.495174065471124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	4990	3.5996133481453696	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	3734	2.693578405205373	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	2562	1.8481381559014902	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	996	0.718479938828214	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	852	0.6146033211663036	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	747	0.5388599541211605	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	694	0.500627587898374	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	587	0.4234414900523711	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	445	0.32100760319132055	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGAAGCTTCGTATGCCG	429	0.3094657567844416	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	247	0.17817725390619363	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	242	0.17457042690404398	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	233	0.16807813830017457	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGT	212	0.15292946489114595	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGTGAAGCTTCGTATGC	192	0.13850215688254727	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTAT	182	0.13128850287824795	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	178	0.1284030412765282	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	178	0.1284030412765282	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTT	177	0.12768167587609827	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	167	0.12046802187179893	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	158	0.11397573326792952	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	156	0.11253300246706965	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	139	0.1002697906597608	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	139	0.1002697906597608	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2221805433324196	0.0
2	0.0	0.0	0.0	1.3107209325811897	0.0
3	0.0	0.0	0.0	1.5711338421363958	0.0
4	0.0	0.0	0.0	2.560125806125835	0.0
5	0.0	0.0	0.0	5.063263745617705	0.0
6	0.0	0.0	0.0	5.468671100659328	0.0
7	0.0	0.0	0.0	6.0775034986221925	0.0
8	0.0	0.0	0.0	6.97055386435445	0.0
9	0.0	0.0	0.0	7.169650714873112	0.0
10	0.0	0.0	0.0	12.432732676409909	0.0
11	0.0	0.0	0.0	13.229841443884986	0.0
12	0.0	0.0	0.0	17.055963527765353	0.0
13	0.0	0.0	0.0	17.39861209296957	0.0
14	0.0	0.0	0.0	17.63738404051188	0.0
15	0.0	0.0	0.0	18.330616190325046	0.0
16	0.0	0.0	0.0	18.716546679555062	0.0
17	0.0	0.0	0.0	19.108969457388945	0.0
18	0.0	0.0	0.0	19.466045330601762	0.0
19	0.0	0.0	0.0	20.662069164514595	0.0
20	0.0	0.0	0.0	21.063869692554068	0.0
21	0.0	0.0	0.0	21.46206339359139	0.0
22	0.0	0.0	0.0	22.190642448025624	0.0
23	0.0	0.0	0.0	22.598935264668967	0.0
24	0.0	0.0	0.0	22.836985846810844	0.0
25	0.0	0.0	0.0	23.031754504926926	0.0
26	0.0	0.0	0.0	23.246000028854617	0.0
27	0.0	0.0	0.0	23.477558322392625	0.0
28	0.0	0.0	0.0	23.62760232568205	0.0
29	0.0	0.0	0.0	23.828863272402003	0.0
30	0.0	0.0	0.0	24.016418276513786	0.0
31	0.0	0.0	0.0	24.240762916047494	0.0
32	0.0	0.0	0.0	24.423268362356268	0.0
33	0.0	0.0	0.0	24.62380794367579	0.0
34	0.0	0.0	0.0	24.81713387099101	0.0
35	0.0	0.0	0.0	25.012623894507524	0.0
36	0.0	0.0	0.0	25.219655764430915	0.0
37	0.0	0.0	0.0	25.392062095133667	0.0
38	0.0	0.0	0.0	25.557254771832124	0.0
39	0.0	0.0	0.0	25.796748084774862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	45	3.8016879E-10	45.000004	17
CTCGTAA	35	1.2016244E-7	45.000004	33
ATGACTC	45	3.8016879E-10	45.000004	3
TCGTAAC	35	1.2016244E-7	45.000004	34
CGTAACC	35	1.2016244E-7	45.000004	35
CCGGTGT	35	1.2016244E-7	45.000004	18
CACCGGT	70	0.0	45.000004	16
GATAAAA	40	6.7411747E-9	45.0	19
ACTGACG	20	7.011811E-4	45.0	28
ACCGCTG	25	3.8730162E-5	45.0	17
TCCCTTG	25	3.8730162E-5	45.0	1
CCGTGTC	20	7.011811E-4	45.0	19
GGATCCT	25	3.8730162E-5	45.0	8
GAATAGC	40	6.7411747E-9	45.0	27
CGCCTCT	20	7.011811E-4	45.0	16
AACATGC	40	6.7411747E-9	45.0	43
GGGGTAT	25	3.8730162E-5	45.0	7
GCGGGAT	20	7.011811E-4	45.0	5
CTGTTGG	55	1.8189894E-12	45.0	2
CGAATAG	40	6.7411747E-9	45.0	26
>>END_MODULE