FastQCFastQC Report
Sat 14 Jan 2017
SRR2933469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151034
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104436.914337169114239No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG55303.6614272283062093No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC41162.725214190182343No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC33752.23459618364077No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC10830.717057086483838No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCAT10710.709111855608671No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT10480.6938834964312671No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC8880.5879470847623713No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTA8570.5674219050015228No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT5880.38931631288319185No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGCCTTTCATCGTATGCCG4410.2919872346623939No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGT3120.20657600275434668No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT3000.19863077187917952No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC2890.19134764357694292No Hit
GAATCTTTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC2760.18274031012884517No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC2700.17876769469126158No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC2580.1708224638160944No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2260.14963518148231525No Hit
GAATCTGTCTCTTATCCACATCTGACGCGCCTTTCATCGTATGCCGTCTTC2240.14831097633645404No Hit
GAATGACTCTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT2220.14698677119059284No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC2160.14301415575300927No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCCTCGTATGCCG2120.14036574546128686No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG2050.13573102745077267No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT1950.1291100017214667No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATG1940.1284478991485361No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1880.1244752837109525No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT1830.12116477084629951No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT1720.11388164254406292No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGCCTTTCATCGTATGC1700.11255743739820173No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT1620.10726061681475695No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1600.10593641166889575No Hit
GAATCTGTCTCTTATCCCCATCTGACGCGCCTTTCATCGTATGCCGTCTTC1580.10461220652303455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACT351.2025703E-745.0000049
CCCGTCT351.2025703E-745.00000418
GGGAACA351.2025703E-745.0000047
TGCTACA453.8016879E-1045.00000412
GCTACAA453.8016879E-1045.00000413
TCCCCAT351.2025703E-745.00000415
TATCCCC351.2025703E-745.00000413
CTAGAGG253.874675E-545.032
CTCGTAA207.013816E-445.033
GGAGATG207.013816E-445.08
GGATGGC253.874675E-545.08
CCAGGAC207.013816E-445.029
CCGGCGA207.013816E-445.08
CGGTTGG207.013816E-445.02
GATCCGT207.013816E-445.05
GATCCGG406.7484507E-945.05
GATCCCT253.874675E-545.05
TTCGTTG207.013816E-445.01
CTCTTCG253.874675E-545.01
TGACGGG253.874675E-545.03
Produced by FastQC (version 0.11.5)