##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151034
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.542189175947136	30.0	25.0	31.0	16.0	33.0
2	27.277970523193453	31.0	25.0	33.0	16.0	34.0
3	27.406014539772503	31.0	25.0	33.0	16.0	34.0
4	31.227948673808548	35.0	28.0	35.0	19.0	37.0
5	33.53062224399804	35.0	33.0	37.0	28.0	37.0
6	31.688255624561357	35.0	32.0	37.0	17.0	37.0
7	32.60540672961055	35.0	32.0	35.0	27.0	37.0
8	32.24281949759657	35.0	32.0	37.0	17.0	37.0
9	33.357416210919396	35.0	32.0	38.0	27.0	39.0
10	32.68079372856443	35.0	30.0	39.0	17.0	39.0
11	33.25729305984083	37.0	31.0	39.0	18.0	39.0
12	33.82522478382351	37.0	31.0	39.0	25.0	39.0
13	33.8458492789703	37.0	32.0	39.0	26.0	39.0
14	33.828780274640145	36.0	32.0	40.0	21.0	41.0
15	34.33399764291484	37.0	32.0	40.0	25.0	41.0
16	34.518386588450284	37.0	32.0	40.0	25.0	41.0
17	32.359071467351725	35.0	27.0	40.0	18.0	41.0
18	33.025682958803976	36.0	30.0	39.0	23.0	39.0
19	32.83475243984798	36.0	31.0	37.0	23.0	39.0
20	31.98246090284307	34.0	30.0	35.0	23.0	39.0
21	32.5789160056676	35.0	31.0	36.0	24.0	39.0
22	32.66598249400797	34.0	30.0	37.0	25.0	39.0
23	33.48449355774196	35.0	32.0	38.0	26.0	39.0
24	32.142557304977686	35.0	31.0	37.0	21.0	39.0
25	31.31232040467709	34.0	29.0	36.0	18.0	39.0
26	30.27952646423984	33.0	25.0	36.0	18.0	39.0
27	32.1880834778924	34.0	30.0	37.0	22.0	39.0
28	32.0214852284916	35.0	30.0	37.0	21.0	39.0
29	31.92873127904975	35.0	30.0	37.0	21.0	39.0
30	31.236728153925608	34.0	29.0	37.0	18.0	39.0
31	31.53321768608393	34.0	30.0	36.0	20.0	39.0
32	31.40860336083266	35.0	30.0	37.0	18.0	39.0
33	30.984791503899785	35.0	30.0	37.0	15.0	40.0
34	29.972827310406927	35.0	25.0	37.0	10.0	40.0
35	29.230047538964737	34.0	23.0	37.0	8.0	40.0
36	28.45334825271131	34.0	21.0	37.0	7.0	40.0
37	28.313061959558773	34.0	18.0	38.0	7.0	40.0
38	28.08317994623727	33.0	18.0	37.0	7.0	40.0
39	27.744852152495465	33.0	18.0	37.0	7.0	40.0
40	27.513500271462053	33.0	18.0	37.0	7.0	39.0
41	26.75833256088033	32.0	17.0	36.0	7.0	39.0
42	27.331216812108533	33.0	18.0	37.0	7.0	39.0
43	27.15714342465935	33.0	16.0	37.0	7.0	39.0
44	27.172643245891653	33.0	15.0	37.0	7.0	40.0
45	27.319226134512757	33.0	15.0	37.0	7.0	40.0
46	26.85586026987301	33.0	14.0	37.0	7.0	40.0
47	26.801137492220295	33.0	13.0	37.0	7.0	40.0
48	26.684388945535442	33.0	12.0	37.0	7.0	39.0
49	26.62907027556709	33.0	12.0	37.0	7.0	39.0
50	26.630771879179523	33.0	11.0	37.0	7.0	40.0
51	24.903710422818705	30.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	5.0
12	5.0
13	13.0
14	39.0
15	73.0
16	163.0
17	383.0
18	614.0
19	1027.0
20	1516.0
21	2263.0
22	3088.0
23	4270.0
24	5858.0
25	7648.0
26	9948.0
27	10294.0
28	9283.0
29	8959.0
30	9883.0
31	10951.0
32	12170.0
33	12899.0
34	13557.0
35	13270.0
36	8299.0
37	3809.0
38	728.0
39	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.236688427771227	2.4034323397380724	26.35697922322126	43.00290000926943
2	44.187401512242275	20.609928890183667	24.716289047499238	10.486380550074816
3	8.533840062502483	20.44903796496153	60.01562562072116	11.001496351814822
4	8.882768118436909	2.037951719480382	76.35300660778368	12.72627355429903
5	22.50221804361932	2.5974283936067377	60.70222598885019	14.198127573923752
6	10.330786445436127	16.71875206907054	63.27780499755023	9.672656487943113
7	38.981288981288984	1.025596885469497	57.07655229948223	2.9165618337592862
8	37.501489730789096	13.780340850404546	43.03997775335355	5.678191665452812
9	34.428009587245256	2.2875643894752176	42.72812744150324	20.55629858177629
10	23.265622310208297	14.51593680893044	47.574056172782285	14.644384708078976
11	20.793993405458373	13.921368698438762	53.56807076552299	11.716567130579868
12	16.824026378166508	11.731795489757273	54.57049406093992	16.8736840711363
13	15.09130394480713	14.288835626415244	61.91586000503197	8.704000423745645
14	11.527205794721718	20.076274216401604	51.08783452732497	17.308685461551704
15	6.832236450070845	14.83705655680178	64.74833481202909	13.582372181098295
16	8.622561807275183	14.870823788021239	52.278294953454186	24.228319451249387
17	9.045645351377836	16.566468477296503	58.16769733967186	16.2201888316538
18	8.602698730087264	15.047605174993711	57.54929353655468	18.800402558364343
19	9.960671107167922	18.62031065852722	52.72720049790114	18.691817736403724
20	11.479534409470716	17.110716792245455	61.19946502112107	10.210283777162758
21	12.184673649641804	22.498245428181736	52.16772382377478	13.149357098401685
22	8.692744680005827	13.841254287114161	56.631619370472876	20.83438166240714
23	10.54464557649271	22.280413681687566	53.670034561754306	13.504906180065415
24	13.132142431505489	15.231669690268415	51.914800640915296	19.721387237310804
25	8.788749553080763	24.068090628600185	50.57867764874134	16.56448216957771
26	10.21359429002741	15.448177231616722	56.247599878173126	18.09062860018274
27	13.077850020525181	17.551677105817234	50.36614272283062	19.004330150826966
28	8.74041606525683	16.29633062754082	58.27495795648662	16.68829535071573
29	17.168981818663347	11.68346200193334	53.44624389210376	17.701312287299547
30	10.004369876981341	19.769058622561808	53.99181641219858	16.234755088258275
31	13.637988797224466	14.194817061059098	49.957625435332446	22.20956870638399
32	15.82292728789544	19.16191056318445	50.82365560072566	14.191506548194447
33	10.611517936358702	15.44685302647086	50.413814108081624	23.527814929088812
34	18.979832355628535	16.64128606803766	47.20791344995167	17.17096812638214
35	10.624759987817313	18.2780036283221	43.266416833295814	27.830819550564772
36	15.74744759458135	19.77634175086404	41.63963081160533	22.83657984294927
37	11.17496722592264	17.94893864957559	49.876848921434906	20.99924520306686
38	13.210270535111299	19.201636717560284	44.901148085861465	22.686944661466953
39	14.91386045526173	18.82026563555226	51.3308261715905	14.935047737595507
40	13.338056331686904	17.424553411814557	47.38999165750758	21.847398598990956
41	13.419494948157368	24.57393699431916	43.22006965319068	18.7864984043328
42	13.535362898420223	18.89706953401221	46.14259041010633	21.424977157461235
43	17.41594607836646	21.518333620244448	39.771177350795185	21.294542950593907
44	17.596037978203583	19.0526636386509	40.76102069732643	22.59027768581909
45	14.364977422302264	18.206496550445593	39.99298171272694	27.435544314525206
46	20.643034018830196	22.676351020300064	37.15057536713587	19.53003959373386
47	12.859356171458083	17.26300038401949	48.58243839135559	21.29520505316684
48	17.411311360355946	19.745885032509236	37.657083835427784	25.18571977170703
49	14.4371466027517	15.290596819259239	45.157381781585606	25.11487479640346
50	16.42941324469987	16.179800574705034	41.995841995842	25.394944184753104
51	16.36585139769853	15.838155647072844	39.55665611716567	28.23933683806295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	85.5
2	164.0
3	7125.0
4	14086.0
5	9837.5
6	5589.0
7	5662.0
8	5735.0
9	5927.5
10	6120.0
11	5716.0
12	5312.0
13	4762.5
14	4213.0
15	3754.5
16	3296.0
17	3038.5
18	2781.0
19	2485.0
20	2189.0
21	2065.5
22	1942.0
23	1869.0
24	1796.0
25	1781.0
26	1707.0
27	1648.0
28	1694.5
29	1741.0
30	1599.0
31	1457.0
32	1606.5
33	1756.0
34	1809.5
35	1863.0
36	1871.5
37	1880.0
38	2140.5
39	2401.0
40	3032.0
41	3663.0
42	4098.0
43	4533.0
44	5339.5
45	6146.0
46	15491.5
47	24837.0
48	19666.5
49	14496.0
50	12589.0
51	10682.0
52	8321.5
53	5961.0
54	4956.5
55	3952.0
56	3300.0
57	2648.0
58	2285.0
59	1922.0
60	1744.5
61	1567.0
62	1279.5
63	992.0
64	857.5
65	723.0
66	572.5
67	422.0
68	344.0
69	266.0
70	232.0
71	198.0
72	158.5
73	119.0
74	92.0
75	56.5
76	48.0
77	35.5
78	23.0
79	21.0
80	19.0
81	12.5
82	6.0
83	3.5
84	1.0
85	2.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	151034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.641590635221206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5548606768119	40.75969649218057
2	5.969008408032798	5.687461101473841
3	2.3737057883399344	3.392613583696386
4	1.3772496699325967	2.624574599096892
5	0.9325272739906887	2.2213541321821575
6	0.7129455909943715	2.037951719480382
7	0.5378361475922452	1.7936358700689912
8	0.43360433604336046	1.6526080220347734
9	0.36550621916475573	1.5671967901267263
>10	1.6093391703147804	12.175404213620775
>50	0.0639288444166493	2.110120899929817
>100	0.05559029949273852	4.777070063694268
>500	0.004169272461955389	1.544685302647086
>1k	0.006948787436592315	7.079862812346889
>5k	0.001389757487318463	3.6614272283062093
>10k+	0.001389757487318463	6.914337169114239
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10443	6.914337169114239	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	5530	3.6614272283062093	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	4116	2.725214190182343	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	3375	2.23459618364077	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	1083	0.717057086483838	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCAT	1071	0.709111855608671	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	1048	0.6938834964312671	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	888	0.5879470847623713	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTA	857	0.5674219050015228	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	588	0.38931631288319185	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGCCTTTCATCGTATGCCG	441	0.2919872346623939	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGT	312	0.20657600275434668	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	300	0.19863077187917952	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	289	0.19134764357694292	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	276	0.18274031012884517	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	270	0.17876769469126158	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	258	0.1708224638160944	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	226	0.14963518148231525	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	224	0.14831097633645404	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	222	0.14698677119059284	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	216	0.14301415575300927	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCCTCGTATGCCG	212	0.14036574546128686	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	205	0.13573102745077267	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT	195	0.1291100017214667	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATG	194	0.1284478991485361	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	188	0.1244752837109525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	183	0.12116477084629951	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT	172	0.11388164254406292	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGCCTTTCATCGTATGC	170	0.11255743739820173	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT	162	0.10726061681475695	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	160	0.10593641166889575	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCGCCTTTCATCGTATGCCGTCTTC	158	0.10461220652303455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.621025729305984E-4	0.0	0.0	0.2449779519843214	0.0
2	6.621025729305984E-4	0.0	0.0	1.5301190460426128	0.0
3	6.621025729305984E-4	0.0	0.0	1.8452798707575777	0.0
4	6.621025729305984E-4	0.0	0.0	3.282704556589907	0.0
5	6.621025729305984E-4	0.0	0.0	6.511778804772435	0.0
6	6.621025729305984E-4	0.0	0.0	7.110981633274627	0.0
7	6.621025729305984E-4	0.0	0.0	7.91146364394772	0.0
8	6.621025729305984E-4	0.0	0.0	9.067494736284544	0.0
9	6.621025729305984E-4	0.0	0.0	9.336308380894367	0.0
10	6.621025729305984E-4	0.0	0.0	14.776143120092165	0.0
11	6.621025729305984E-4	0.0	0.0	16.115576625130764	0.0
12	6.621025729305984E-4	0.0	0.0	20.266959757405616	0.0
13	6.621025729305984E-4	0.0	0.0	20.704609558112743	0.0
14	6.621025729305984E-4	0.0	0.0	20.959519048691025	0.0
15	6.621025729305984E-4	0.0	0.0	21.882490035356277	0.0
16	6.621025729305984E-4	0.0	0.0	22.436007786326257	0.0
17	6.621025729305984E-4	0.0	0.0	22.96767615238953	0.0
18	6.621025729305984E-4	0.0	0.0	23.474184620681434	0.0
19	6.621025729305984E-4	0.0	0.0	24.7652846378961	0.0
20	6.621025729305984E-4	0.0	0.0	25.280400439636107	0.0
21	6.621025729305984E-4	0.0	0.0	25.853781267794005	0.0
22	6.621025729305984E-4	0.0	0.0	26.960154667161035	0.0
23	6.621025729305984E-4	0.0	0.0	27.467325238025875	0.0
24	6.621025729305984E-4	0.0	0.0	27.80433544764755	0.0
25	6.621025729305984E-4	0.0	0.0	28.110226836341486	0.0
26	6.621025729305984E-4	0.0	0.0	28.39493094270164	0.0
27	6.621025729305984E-4	0.0	0.0	28.65513725386337	0.0
28	6.621025729305984E-4	0.0	0.0	28.898791000701827	0.0
29	6.621025729305984E-4	0.0	0.0	29.156348901571832	0.0
30	6.621025729305984E-4	0.0	0.0	29.44303931565078	0.0
31	6.621025729305984E-4	0.0	0.0	29.6966246010832	0.0
32	6.621025729305984E-4	0.0	0.0	29.93762993762994	0.0
33	6.621025729305984E-4	0.0	0.0	30.14685435067601	0.0
34	6.621025729305984E-4	0.0	0.0	30.397129123243772	0.0
35	6.621025729305984E-4	0.0	0.0	30.701034204218917	0.0
36	6.621025729305984E-4	0.0	0.0	30.945350053630307	0.0
37	6.621025729305984E-4	0.0	0.0	31.173113338718434	0.0
38	6.621025729305984E-4	0.0	0.0	31.41014605982759	0.0
39	6.621025729305984E-4	0.0	0.0	31.6657176529788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACT	35	1.2025703E-7	45.000004	9
CCCGTCT	35	1.2025703E-7	45.000004	18
GGGAACA	35	1.2025703E-7	45.000004	7
TGCTACA	45	3.8016879E-10	45.000004	12
GCTACAA	45	3.8016879E-10	45.000004	13
TCCCCAT	35	1.2025703E-7	45.000004	15
TATCCCC	35	1.2025703E-7	45.000004	13
CTAGAGG	25	3.874675E-5	45.0	32
CTCGTAA	20	7.013816E-4	45.0	33
GGAGATG	20	7.013816E-4	45.0	8
GGATGGC	25	3.874675E-5	45.0	8
CCAGGAC	20	7.013816E-4	45.0	29
CCGGCGA	20	7.013816E-4	45.0	8
CGGTTGG	20	7.013816E-4	45.0	2
GATCCGT	20	7.013816E-4	45.0	5
GATCCGG	40	6.7484507E-9	45.0	5
GATCCCT	25	3.874675E-5	45.0	5
TTCGTTG	20	7.013816E-4	45.0	1
CTCTTCG	25	3.874675E-5	45.0	1
TGACGGG	25	3.874675E-5	45.0	3
>>END_MODULE