##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933468.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907373
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.24666151626729	31.0	28.0	31.0	25.0	34.0
2	28.257031011502438	31.0	26.0	33.0	16.0	34.0
3	28.23289099411157	31.0	25.0	33.0	16.0	34.0
4	32.96110860693452	35.0	32.0	37.0	28.0	37.0
5	34.16502474726491	35.0	33.0	37.0	30.0	37.0
6	33.46979136474195	35.0	33.0	37.0	28.0	37.0
7	33.58996024788042	35.0	32.0	37.0	29.0	37.0
8	33.38361401540491	35.0	33.0	37.0	28.0	37.0
9	34.64906824426118	37.0	34.0	39.0	28.0	39.0
10	34.57453219348603	37.0	32.0	39.0	27.0	39.0
11	34.92104680214201	37.0	34.0	39.0	28.0	39.0
12	35.024693262858825	37.0	34.0	39.0	27.0	39.0
13	35.10325191514405	37.0	34.0	39.0	28.0	39.0
14	35.909903644917804	38.0	34.0	40.0	27.0	41.0
15	36.13448603826651	38.0	34.0	40.0	27.0	41.0
16	36.134762661000494	38.0	34.0	40.0	30.0	41.0
17	35.39947739242847	37.0	33.0	40.0	27.0	41.0
18	35.196424182778195	37.0	33.0	39.0	27.0	40.0
19	34.55513884587705	37.0	33.0	38.0	27.0	40.0
20	33.66293134135576	35.0	32.0	38.0	26.0	40.0
21	34.1717364303324	35.0	33.0	38.0	27.0	40.0
22	34.71153318425829	35.0	33.0	39.0	29.0	40.0
23	34.95690636595975	35.0	33.0	39.0	29.0	40.0
24	34.48304170390787	35.0	33.0	39.0	27.0	40.0
25	33.4668344771114	35.0	32.0	38.0	24.0	40.0
26	33.31485948997821	35.0	32.0	38.0	24.0	40.0
27	34.162040307569214	35.0	33.0	38.0	26.0	40.0
28	33.66716003231306	35.0	33.0	38.0	24.0	40.0
29	33.784739021328605	35.0	33.0	39.0	24.0	40.0
30	33.07198583162602	35.0	32.0	39.0	21.0	40.0
31	32.90752314648992	35.0	32.0	38.0	21.0	40.0
32	32.36530842332756	35.0	31.0	38.0	16.0	40.0
33	31.41044642060101	35.0	30.0	39.0	12.0	40.0
34	31.02386449673949	35.0	27.0	39.0	8.0	40.0
35	30.50056702150053	35.0	25.0	39.0	7.0	40.0
36	29.967617506802604	35.0	23.0	39.0	7.0	40.0
37	30.074110646889427	35.0	23.0	39.0	7.0	40.0
38	29.694382574751508	35.0	22.0	39.0	7.0	40.0
39	29.365050535997874	35.0	21.0	39.0	7.0	40.0
40	29.237071193434232	35.0	21.0	39.0	7.0	40.0
41	28.444016958847133	34.0	18.0	38.0	7.0	40.0
42	28.94479006979489	35.0	19.0	39.0	7.0	40.0
43	28.859627738537515	35.0	18.0	39.0	7.0	40.0
44	29.03808246443304	35.0	18.0	39.0	7.0	40.0
45	29.16640455468699	35.0	20.0	39.0	7.0	40.0
46	28.919913861223556	35.0	20.0	39.0	7.0	40.0
47	28.861305108263085	35.0	20.0	39.0	7.0	40.0
48	28.66611746216826	35.0	20.0	38.0	7.0	40.0
49	28.876262573384924	35.0	20.0	39.0	7.0	40.0
50	28.851635435482432	35.0	20.0	39.0	7.0	40.0
51	27.52428934958391	33.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	46.0
10	43.0
11	63.0
12	86.0
13	100.0
14	158.0
15	213.0
16	502.0
17	946.0
18	1914.0
19	3658.0
20	6099.0
21	9082.0
22	13281.0
23	18958.0
24	27731.0
25	39462.0
26	48740.0
27	48352.0
28	43490.0
29	40736.0
30	41746.0
31	46435.0
32	53751.0
33	61795.0
34	70080.0
35	80132.0
36	86701.0
37	87068.0
38	66151.0
39	9830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.460982418476195	2.3258351306463823	30.168519451206944	38.04466299967048
2	37.90646184094083	19.561856039357576	29.61604544106999	12.915636678631609
3	10.98412670423299	19.857434594152572	56.04167194747915	13.11676675413529
4	9.98167236627054	2.596065785514887	72.10485654741765	15.317405300796915
5	22.342961494335846	3.0313884146872345	57.533561170543976	17.092088920432943
6	12.904175019534414	15.896990543029164	61.21363540682828	9.98519903060814
7	49.788455243874346	1.0750815816648722	46.49741616733141	2.6390470071293723
8	47.67025247610409	11.97258459310559	36.54351628271946	3.813646648070859
9	44.82842226956279	2.5225568757280636	35.05151685139408	17.597504003315066
10	28.488284310862237	15.796921442449797	42.26729250264224	13.447501744045724
11	22.9029296661902	15.069216298038404	47.70794370121218	14.319910334559216
12	17.66649437441934	13.117428003698588	50.88161098026942	18.334466641612657
13	16.656435666478945	14.584079535097475	57.37122440275388	11.388260395669697
14	14.607664102855164	21.439804799128915	48.27595707608668	15.676574021929241
15	11.073505603539008	15.458802499082516	58.86278300103706	14.604908896341417
16	13.418076138478884	17.942015025794245	46.690721456336036	21.949187379390835
17	13.82441399512659	18.00483373430772	52.04078146473391	16.129970805831782
18	14.097069231727193	17.027066046708462	50.61986636146326	18.255998360101085
19	13.931205799599503	19.31697328441556	46.975499601597136	19.776321314387797
20	15.711620248784127	19.3413293099971	52.987470422858074	11.959580018360697
21	15.487126022043856	22.865458857603212	49.19002438908806	12.457390731264871
22	13.679820757285041	14.128258169462834	51.93277736939494	20.25914370385718
23	14.436290257699977	21.62010551338865	50.75795731193236	13.185646916979016
24	16.606952157492014	17.58934859203437	46.92480380174416	18.878895448729462
25	12.923902298172857	26.551925172999418	45.195415777194164	15.328756751633563
26	13.640807033050356	15.507734966766698	50.79454645443494	20.056911545747997
27	17.30159482373842	18.308347283862314	45.72419501131288	18.66586288108639
28	11.646919183180456	18.901928975184408	51.43739123822287	18.01376060341227
29	16.44968496968722	15.281587616118179	49.75043339398461	18.518294020209993
30	13.464032983128218	21.002608629527217	47.39396036690534	18.139398020439224
31	16.02284837657722	19.74083425449071	44.3742540278364	19.862063341095666
32	17.735705162044717	20.020101986724313	42.029793701157075	20.214399150073895
33	14.79667126969835	17.622411070199355	40.645357532128465	26.935560127973833
34	16.740083736236365	19.806187752996838	46.40142477239239	17.052303738374405
35	13.890428743196019	20.65170552793614	38.09249338474916	27.365372344118683
36	16.028358789604717	23.608703366752152	41.9687383248124	18.394199518830735
37	15.268913666154932	21.38613337624108	39.361541504981965	23.98341145262202
38	17.52168072005669	24.118747196577374	34.25713570934995	24.10243637401598
39	19.304299334452317	20.886449122907557	39.19578828111482	20.613463261525304
40	17.481124080174308	20.99180821999332	37.121117776261805	24.40594992357057
41	17.110714116465886	24.43482448783466	39.690953995765796	18.763507399933655
42	16.905616543582408	21.838097452756475	40.09200185590711	21.16428414775401
43	19.739511755364113	20.659530314435187	37.81256440295226	21.78839352724844
44	20.151249816778765	21.320449252953306	35.19159155055308	23.336709379714847
45	16.910906540088806	21.56411971702927	35.42159619032085	26.103377552561074
46	22.467937661799503	23.509185307475537	33.5797957400099	20.443081290715064
47	14.96517970007924	20.838398321307775	43.196127722557314	21.000294256055668
48	18.881540446982665	23.947042726640532	33.859394097025145	23.312022729351657
49	16.660182747337643	20.773265239322747	39.62978841116057	22.936763602179038
50	17.72038621382827	20.51008791312944	37.978427835079955	23.79109803796234
51	17.9231694132402	21.07766045496174	34.47766243871043	26.521507693087624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	1216.5
2	2365.0
3	27958.0
4	53551.0
5	40211.5
6	26872.0
7	25880.0
8	24888.0
9	24266.0
10	23644.0
11	22987.5
12	22331.0
13	21282.0
14	20233.0
15	19092.0
16	17951.0
17	17006.0
18	16061.0
19	15128.5
20	14196.0
21	13358.0
22	12520.0
23	11872.5
24	11225.0
25	11161.0
26	11343.0
27	11589.0
28	12042.5
29	12496.0
30	13573.0
31	14650.0
32	15763.0
33	16876.0
34	18335.0
35	19794.0
36	21116.0
37	22438.0
38	23735.5
39	25033.0
40	25928.5
41	26824.0
42	28861.5
43	30899.0
44	34092.5
45	37286.0
46	95334.0
47	153382.0
48	105711.5
49	58041.0
50	52585.5
51	47130.0
52	41014.5
53	34899.0
54	33225.0
55	31551.0
56	29558.5
57	27566.0
58	25582.5
59	23599.0
60	20746.5
61	17894.0
62	15199.0
63	12504.0
64	10571.5
65	8639.0
66	7328.5
67	6018.0
68	5153.0
69	4288.0
70	3454.0
71	2620.0
72	2181.5
73	1743.0
74	1484.0
75	940.5
76	656.0
77	493.0
78	330.0
79	264.5
80	199.0
81	152.5
82	106.0
83	73.5
84	41.0
85	34.0
86	27.0
87	24.0
88	21.0
89	12.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	907373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.55978118950097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.92537978490351	38.23621939245972
2	5.820295383334623	5.303427682459763
3	2.476388868965694	3.384712050305785
4	1.51015042309267	2.7520849135733743
5	1.149996768042426	2.6196800560323115
6	0.864014277678977	2.3618580861395357
7	0.7333812218995732	2.338888159876339
8	0.5956657480410551	2.1710720914264714
9	0.48769052259766366	1.9997166147968641
>10	2.327293554017652	15.865773962732066
>50	0.0635486930302646	2.036147905749293
>100	0.03983992045856793	3.4738527016050664
>500	0.002933000278811862	0.834054261230241
>1k	0.0017109168293069196	2.2910323592190895
>5k	7.332500697029655E-4	2.126448621155231
>10k+	9.77666759603954E-4	12.205031141238896
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	30884	3.4036719188250033	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCG	28705	3.163528119086638	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27967	3.0821944228007667	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC	22048	2.429871728605546	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCT	7787	0.8581917249025484	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTC	6050	0.6667599763272657	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCTCTT	5259	0.5795852422322463	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	4964	0.5470738053700077	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCT	4871	0.5368244371388613	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTA	4065	0.4479965791355925	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTT	2638	0.2907293913307978	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGT	1856	0.2045465315807281	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATG	1117	0.12310262703430673	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTAT	1063	0.11715138096460881	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07780703194827265	0.0
2	0.0	0.0	0.0	0.7239580635526955	0.0
3	0.0	0.0	0.0	0.8480525649319519	0.0
4	0.0	0.0	0.0	1.9236851878995738	0.0
5	0.0	0.0	0.0	6.094627016673408	0.0
6	0.0	0.0	0.0	6.550779007089697	0.0
7	0.0	0.0	0.0	7.31165683792663	0.0
8	0.0	0.0	0.0	8.215144157915212	0.0
9	0.0	0.0	0.0	8.372741970501657	0.0
10	0.0	0.0	0.0	12.20446277330271	0.0
11	0.0	0.0	0.0	12.437332827844779	0.0
12	0.0	0.0	0.0	15.540025987107837	0.0
13	0.0	0.0	0.0	15.800778731569046	0.0
14	0.0	0.0	0.0	15.993202354489279	0.0
15	0.0	0.0	0.0	16.61676069268096	0.0
16	0.0	0.0	0.0	16.943638393472146	0.0
17	0.0	0.0	0.0	17.112697865155784	0.0
18	0.0	0.0	0.0	17.24461715303409	0.0
19	0.0	0.0	0.0	18.078893685397297	0.0
20	0.0	0.0	0.0	18.26547627050838	0.0
21	0.0	0.0	0.0	18.415579921377425	0.0
22	0.0	0.0	0.0	18.727248882212717	0.0
23	0.0	0.0	0.0	18.886720235228513	1.1020826054996126E-4
24	0.0	0.0	0.0	19.03461972088656	1.1020826054996126E-4
25	0.0	0.0	0.0	19.163783802251114	1.1020826054996126E-4
26	0.0	0.0	0.0	19.31961828266876	1.1020826054996126E-4
27	0.0	0.0	0.0	19.532981475093486	1.1020826054996126E-4
28	0.0	0.0	0.0	19.662586389500238	1.1020826054996126E-4
29	0.0	0.0	0.0	19.815555455143585	1.1020826054996126E-4
30	0.0	0.0	0.0	20.004231997205117	1.1020826054996126E-4
31	1.1020826054996126E-4	0.0	0.0	20.167781055861262	1.1020826054996126E-4
32	1.1020826054996126E-4	0.0	0.0	20.344665314043947	1.1020826054996126E-4
33	1.1020826054996126E-4	0.0	0.0	20.51240228660099	1.1020826054996126E-4
34	1.1020826054996126E-4	0.0	0.0	20.734692348130263	1.1020826054996126E-4
35	1.1020826054996126E-4	0.0	0.0	20.935712215373393	1.1020826054996126E-4
36	1.1020826054996126E-4	0.0	0.0	21.111384182690028	1.1020826054996126E-4
37	1.1020826054996126E-4	0.0	0.0	21.312734674714807	1.1020826054996126E-4
38	1.1020826054996126E-4	0.0	0.0	21.54097598231378	1.1020826054996126E-4
39	1.1020826054996126E-4	0.0	0.0	21.907308240381848	1.1020826054996126E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	20	7.032502E-4	45.0	20
TCGATAT	20	7.032502E-4	45.0	42
TGCGAGG	25	3.8901548E-5	45.0	2
CGACTAT	20	7.032502E-4	45.0	19
CGTTTTT	24210	0.0	44.34944	1
CCGATGA	815	0.0	43.067486	18
ACCCGTC	115	0.0	43.04348	17
ACGGCTG	2745	0.0	42.62295	8
GATACCT	3425	0.0	42.569344	5
TACGGCT	2765	0.0	42.314648	7
TGATACC	3440	0.0	42.1875	4
CGGCTGT	2790	0.0	41.85484	9
CGATGAA	840	0.0	41.51786	19
ATACCTG	3545	0.0	41.191822	6
GAATCTG	3855	0.0	41.031128	1
CGGGTAC	55	6.002665E-11	40.909092	6
TACCTGT	3620	0.0	40.587013	7
CGACGAA	50	1.0804797E-9	40.5	19
GACGAAT	50	1.0804797E-9	40.5	20
GATGAAT	865	0.0	40.317917	20
>>END_MODULE