##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933467.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597842
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.213263705126103	31.0	26.0	31.0	16.0	33.0
2	28.293060708347692	31.0	28.0	33.0	16.0	34.0
3	28.45276009380405	31.0	28.0	33.0	16.0	34.0
4	31.772650633444957	35.0	28.0	37.0	19.0	37.0
5	33.570174059366856	35.0	33.0	37.0	28.0	37.0
6	32.454889418943466	35.0	32.0	37.0	17.0	37.0
7	32.193360453096304	35.0	32.0	35.0	25.0	37.0
8	32.78186377002619	35.0	32.0	35.0	26.0	37.0
9	32.96380147262989	35.0	31.0	37.0	25.0	39.0
10	31.729809882878754	35.0	28.0	39.0	15.0	39.0
11	32.528060925796446	35.0	30.0	39.0	17.0	39.0
12	33.258661987615454	35.0	31.0	39.0	23.0	39.0
13	33.39391678737861	35.0	32.0	39.0	25.0	39.0
14	33.59370201491364	36.0	32.0	40.0	21.0	41.0
15	34.0650707042998	36.0	32.0	40.0	25.0	41.0
16	34.231106212009195	37.0	32.0	40.0	25.0	41.0
17	32.27061497853948	35.0	27.0	39.0	18.0	41.0
18	32.94752794216532	36.0	30.0	39.0	23.0	39.0
19	32.73185055583248	36.0	30.0	37.0	21.0	39.0
20	32.212827134928624	34.0	30.0	36.0	23.0	39.0
21	32.67447921022611	35.0	31.0	37.0	24.0	39.0
22	32.98008169382546	35.0	31.0	37.0	25.0	39.0
23	33.903425988806404	35.0	32.0	38.0	27.0	40.0
24	32.281995911963364	35.0	31.0	37.0	21.0	39.0
25	31.423396148146164	34.0	29.0	37.0	18.0	39.0
26	30.582588710729592	33.0	25.0	37.0	18.0	39.0
27	32.40776827322269	34.0	30.0	37.0	22.0	39.0
28	32.17481541945865	35.0	31.0	37.0	21.0	39.0
29	32.470403551440015	35.0	31.0	38.0	21.0	40.0
30	31.49050585271694	34.0	29.0	37.0	18.0	40.0
31	31.51901338480736	34.0	30.0	37.0	18.0	39.0
32	31.512277491377322	35.0	30.0	37.0	18.0	39.0
33	31.08557444943647	35.0	29.0	38.0	15.0	40.0
34	30.368366558388338	35.0	27.0	38.0	10.0	40.0
35	29.82962388055707	35.0	25.0	38.0	8.0	40.0
36	29.09857286707859	34.0	23.0	38.0	7.0	40.0
37	29.382385312507317	35.0	23.0	38.0	7.0	40.0
38	29.064691005315787	34.0	22.0	38.0	7.0	40.0
39	28.64549830891774	34.0	22.0	37.0	7.0	40.0
40	28.474080108122212	33.0	21.0	37.0	7.0	40.0
41	27.549454538155565	33.0	18.0	37.0	7.0	40.0
42	28.373409696876433	33.0	21.0	37.0	7.0	40.0
43	28.355501955366133	33.0	21.0	38.0	7.0	40.0
44	28.447772153846667	34.0	20.0	38.0	7.0	40.0
45	28.700477718193103	34.0	20.0	38.0	7.0	40.0
46	28.39283790700553	34.0	20.0	38.0	7.0	40.0
47	28.501624174949235	34.0	20.0	38.0	7.0	40.0
48	28.255816754259488	34.0	20.0	37.0	7.0	40.0
49	28.19114414845394	34.0	20.0	37.0	7.0	40.0
50	28.33448302394278	34.0	20.0	38.0	7.0	40.0
51	26.576105392394645	31.0	18.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	17.0
10	34.0
11	39.0
12	41.0
13	67.0
14	125.0
15	246.0
16	508.0
17	1075.0
18	2029.0
19	3377.0
20	5436.0
21	7701.0
22	10953.0
23	15204.0
24	20727.0
25	28021.0
26	34253.0
27	35337.0
28	33709.0
29	34309.0
30	38017.0
31	43000.0
32	48483.0
33	53223.0
34	57144.0
35	57385.0
36	41437.0
37	21473.0
38	4390.0
39	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.1138260610663	2.381732966235226	33.07228331231329	39.43215766038519
2	40.64317997062769	14.64601014983892	31.524717232981292	13.1860926465521
3	11.665456759478257	14.637981272643943	60.254883397285575	13.44167857059223
4	10.673221352798901	2.6376534268251475	70.5077595752724	16.181365645103558
5	19.626255766573777	3.08844142766818	60.370465775238266	16.91483703051977
6	13.526149049414393	11.829045132325932	63.28611907493953	11.358686743320142
7	50.38973508050623	1.0989525660626052	45.47723311510399	3.034079238327183
8	50.2313320241803	8.818550720758997	36.563841282479316	4.386275972581384
9	46.279619029777095	2.4929663690406496	36.267943704189406	14.959470896992851
10	26.921661576135502	16.37355689295834	42.7761850120935	13.928596518812665
11	21.124310436536746	15.40674626406308	49.37391484706662	14.09502845233356
12	17.843845029288673	13.546388510676735	51.670173724830306	16.939592735204283
13	16.063943316127002	14.889050953261899	57.19721933219814	11.84978639841296
14	14.364999448014693	18.395161263343827	50.60082764342418	16.6390116452173
15	11.159804764469543	15.782932614302775	58.306709799579146	14.75055282164853
16	13.400697843242865	16.34027050625416	48.666704580808975	21.592327069694
17	13.827566480775857	16.75359041352063	52.69536098166405	16.723482124039464
18	13.936959932557432	15.817724415481013	51.954864328702236	18.290451323259322
19	14.002696364591314	18.94748110704835	48.216418384790636	18.833404143569705
20	15.183443117077754	18.862007018576815	53.27962906587359	12.674920798471836
21	15.547418883250089	21.049039712833824	49.82921909133182	13.574322312584261
22	13.611455869611033	14.820470960554797	51.942486476359974	19.625586693474194
23	13.889455742487144	20.906694410897863	50.604507545471876	14.599342301143112
24	15.470642745073112	17.017707019580424	48.54342786221109	18.968222373135376
25	13.217037277407742	22.61366715620515	46.93815422804018	17.23114133834692
26	13.48433198069055	16.233887883420703	51.14043509823666	19.141345037652087
27	15.270255351748455	17.90657063237444	47.73384941171748	19.08932460415963
28	11.733702215635569	18.14777148477357	52.09871504511226	18.019811254478608
29	15.75165344689734	15.218903991355576	50.083132332623	18.946310229124084
30	13.61848113715664	19.4987973411035	48.674566189729056	18.208155332010797
31	15.061337276404133	18.75729707849231	46.29116054074488	19.890205104358678
32	16.48094312544117	19.3904074989713	44.31572221423052	19.812927161357013
33	14.070105479374147	17.195345927519313	43.62306428788877	25.11148430521777
34	15.492722157359301	19.345914137849128	47.062100019737656	18.09926368505391
35	12.788997761950483	19.975010119730634	40.94259018269041	26.29340193562848
36	15.182941312253071	22.00380702593662	43.04197430090225	19.771277360908066
37	14.653704490484106	21.038669079790314	40.8198152689172	23.487811160808374
38	16.589834772398056	22.42816663934618	37.46541728416538	23.51658130409038
39	17.607327688586615	20.02719782149799	41.582224065890316	20.783250424025077
40	16.90547000712563	19.943730952325197	39.5616233051542	23.58917573539497
41	16.10960755517344	22.10533886879811	42.27320930948311	19.511844266545342
42	16.452172982159166	20.619829319452297	42.105773766312836	20.822223932075698
43	18.11950314631625	19.69885019787837	40.52492130027666	21.65672535552872
44	19.221801077876762	20.38615553942346	37.69909106419422	22.692952318505558
45	16.565246335988437	20.364577931961954	37.991141472161544	25.07903425988807
46	19.87314374031935	22.22041944192613	36.63626844550901	21.27016837224551
47	14.707230338450628	19.89957212775282	43.94505571706237	21.448141816734186
48	17.7344515775071	22.057834678727826	37.16031995075622	23.047393793008855
49	15.879948213742093	19.568548211734875	40.65221245747204	23.899291117050993
50	16.57929687107965	19.13866874525376	39.96440531110226	24.317629072564323
51	17.094148621207612	19.48842670805999	37.19293726436082	26.224487406371583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	482.0
2	896.0
3	25387.5
4	49879.0
5	34258.5
6	18638.0
7	17656.0
8	16674.0
9	16380.5
10	16087.0
11	15252.0
12	14417.0
13	13623.5
14	12830.0
15	11987.5
16	11145.0
17	10363.5
18	9582.0
19	8946.5
20	8311.0
21	7757.0
22	7203.0
23	6754.0
24	6305.0
25	6183.5
26	6255.5
27	6449.0
28	6744.5
29	7040.0
30	7653.5
31	8267.0
32	9102.0
33	9937.0
34	10876.0
35	11815.0
36	12766.0
37	13717.0
38	14769.0
39	15821.0
40	16656.5
41	17492.0
42	19204.0
43	20916.0
44	23217.0
45	25518.0
46	50511.5
47	75505.0
48	61760.5
49	48016.0
50	43697.0
51	39378.0
52	33351.0
53	27324.0
54	24374.5
55	21425.0
56	19556.5
57	17688.0
58	16184.5
59	14681.0
60	12987.0
61	11293.0
62	9715.0
63	8137.0
64	7034.0
65	5931.0
66	5202.5
67	4474.0
68	3866.0
69	3258.0
70	2628.0
71	1998.0
72	1717.5
73	1437.0
74	1233.0
75	815.0
76	601.0
77	425.0
78	249.0
79	207.0
80	165.0
81	132.5
82	100.0
83	73.0
84	46.0
85	33.0
86	20.0
87	17.5
88	15.0
89	8.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.86966250658947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50370609214457	39.494245661738105
2	6.942495904177467	6.646698717727101
3	2.9238780016940447	4.198951594546054
4	1.7507878757260522	3.352384989265393
5	1.235655278743964	2.9575200583979653
6	0.9278863387946611	2.665056352954522
7	0.7446425021161753	2.4952049685054556
8	0.5834949619639509	2.234536552280767
9	0.484984859016377	2.0894455369727853
>10	1.802763509508853	12.885962131217237
>50	0.051632778814714966	1.6984453629649314
>100	0.040950134736352506	4.093553316802337
>500	0.0028487050251375656	0.8741095735399704
>1k	0.0028487050251375656	2.7211944680171776
>5k	3.560881281421957E-4	1.4843498764403396
>10k+	0.001068264384426587	10.108340838629832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34825	5.825117673231389	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCG	13805	2.309138534930634	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	10671	1.7849197614085328	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC	8708	1.4565721377889143	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCT	3705	0.6197289584873595	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCTCTT	2586	0.43255575887943637	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	2518	0.421181516186551	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTC	2289	0.3828770812355104	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCT	1407	0.23534646277779078	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTA	1310	0.21912144011293952	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCACCTCTTCGTATGCCG	1124	0.18800954098240003	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTT	1025	0.17144998176775803	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	828	0.13849813161336944	No Hit
GAATCTGTCTCTTATCCACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	679	0.11357515865395874	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	678	0.11340789037906336	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGT	675	0.11290608555437724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	633	0.10588081800877154	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.145021594334289	0.0
2	0.0	0.0	0.0	0.8181091325132728	0.0
3	0.0	0.0	0.0	1.076036812401939	0.0
4	0.0	0.0	0.0	2.152073624803878	0.0
5	0.0	0.0	0.0	4.72666691199347	0.0
6	0.0	0.0	0.0	5.396074548124755	0.0
7	0.0	0.0	0.0	6.068660281479053	0.0
8	0.0	0.0	0.0	6.9811087210333165	0.0
9	0.0	0.0	0.0	7.313303514975528	0.0
10	0.0	0.0	0.0	10.639767697819826	0.0
11	0.0	0.0	0.0	11.421579614680802	0.0
12	0.0	0.0	0.0	13.83291906557251	0.0
13	1.672682748953737E-4	0.0	0.0	14.18284429665363	0.0
14	1.672682748953737E-4	0.0	0.0	14.388417006500045	0.0
15	1.672682748953737E-4	0.0	0.0	14.883363831915457	0.0
16	1.672682748953737E-4	0.0	0.0	15.221245747204112	0.0
17	1.672682748953737E-4	0.0	0.0	15.553775077696114	0.0
18	1.672682748953737E-4	0.0	0.0	15.8904861150605	0.0
19	1.672682748953737E-4	0.0	0.0	16.6823341284152	0.0
20	1.672682748953737E-4	0.0	0.0	16.99462399764486	0.0
21	1.672682748953737E-4	0.0	0.0	17.345887374925148	0.0
22	1.672682748953737E-4	0.0	0.0	17.839496054141396	0.0
23	3.345365497907474E-4	0.0	0.0	18.214344258181928	0.0
24	3.345365497907474E-4	0.0	0.0	18.511579982671005	0.0
25	3.345365497907474E-4	0.0	0.0	18.777871076304443	0.0
26	3.345365497907474E-4	0.0	0.0	19.01756651422951	0.0
27	3.345365497907474E-4	0.0	0.0	19.273152438269644	0.0
28	3.345365497907474E-4	0.0	0.0	19.517865924441576	0.0
29	3.345365497907474E-4	0.0	0.0	19.786833310473337	0.0
30	3.345365497907474E-4	0.0	0.0	20.10481030104944	0.0
31	3.345365497907474E-4	0.0	0.0	20.362905249213004	0.0
32	5.01804824686121E-4	0.0	0.0	20.626018245623424	0.0
33	5.01804824686121E-4	0.0	0.0	20.889465778583638	0.0
34	5.01804824686121E-4	0.0	0.0	21.164789359061423	0.0
35	5.01804824686121E-4	0.0	0.0	21.48059186206389	0.0
36	6.690730995814948E-4	0.0	0.0	21.74705022397222	0.0
37	6.690730995814948E-4	0.0	0.0	22.022875609274692	0.0
38	6.690730995814948E-4	0.0	0.0	22.347710599121505	0.0
39	8.363413744768685E-4	0.0	0.0	22.852526252755744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	20	7.030568E-4	45.0	40
CTACGGC	80	0.0	45.0	6
CGCCCTA	20	7.030568E-4	45.0	25
TCAAGCG	65	0.0	44.999996	17
CGTTTTT	17980	0.0	44.474415	1
TACGGCT	1285	0.0	43.249027	7
ATGATAC	4965	0.0	42.280964	3
GAATGAT	5325	0.0	42.21127	1
AATGATA	4945	0.0	42.17897	2
ACGGCTG	1330	0.0	42.12406	8
AATGATC	465	0.0	42.096775	2
ATACGGC	3040	0.0	42.039474	6
TGATACC	1920	0.0	41.718746	4
CACCGGT	135	0.0	41.666664	16
TGGGCGA	655	0.0	41.564884	6
GATACCT	1930	0.0	41.50259	5
TGATACG	3130	0.0	41.40575	4
TGATCCG	125	0.0	41.4	4
ACACGTG	60	3.6379788E-12	41.249996	42
TCGTCCC	60	3.6379788E-12	41.249996	38
>>END_MODULE