SRR2933466.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222985
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	12126	5.43803394847187	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	9707	4.3532076148619865	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	8667	3.8868085297217303	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5468	2.452182882256654	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT	2481	1.1126308944547838	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC	2153	0.9655357983720879	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA	2079	0.9323497096217234	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	2016	0.9040966881180348	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	1941	0.8704621387088817	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	1820	0.8161983989954481	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGC	997	0.44711527681234164	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGT	757	0.33948471870305175	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT	549	0.24620490167500056	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	445	0.19956499316097492	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATG	382	0.17131197165728637	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCG	371	0.1663789044106106	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCCAACT	336	0.1506827813530058	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTCCAACTT	280	0.12556898446083817	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	255	0.11435746799112048	No Hit
GTTCTGGGTCACTGAAGGTGACTACTTTTTGCTTGAATGAATTTCCTAATC	249	0.11166670403838823	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	30	2.157607E-6	45.000004	17
GCGATCT	30	2.157607E-6	45.000004	9
TCACGGG	20	7.0210453E-4	45.0	3
TGATCCG	25	3.880662E-5	45.0	4
GCGACCG	20	7.0210453E-4	45.0	11
AGTCATG	25	3.880662E-5	45.0	26
CTCGTCC	20	7.0210453E-4	45.0	37
TGCTTCG	35	1.2060445E-7	45.0	1
CACGGGG	20	7.0210453E-4	45.0	4
CATTGGG	20	7.0210453E-4	45.0	3
GCTATAA	20	7.0210453E-4	45.0	33
ACCTGGG	20	7.0210453E-4	45.0	3
CCCAGCG	20	7.0210453E-4	45.0	29
GTTAGTC	20	7.0210453E-4	45.0	35
TTCAAGT	20	7.0210453E-4	45.0	22
TACGGTG	35	1.2060445E-7	45.0	7
CCTCGTC	20	7.0210453E-4	45.0	36
TTTCACG	20	7.0210453E-4	45.0	1
TGCATCC	20	7.0210453E-4	45.0	44
CTACGGC	25	3.880662E-5	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content