##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222985
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.852016951812903	30.0	28.0	31.0	25.0	33.0
2	27.362966118797228	31.0	25.0	33.0	16.0	34.0
3	27.35404623629392	31.0	25.0	33.0	16.0	34.0
4	32.587039486961004	35.0	32.0	37.0	28.0	37.0
5	34.072798618741174	35.0	33.0	37.0	30.0	37.0
6	32.77761732851985	35.0	32.0	37.0	28.0	37.0
7	33.54354328766509	35.0	33.0	37.0	29.0	37.0
8	32.842765208422094	35.0	32.0	37.0	26.0	37.0
9	34.571953270399355	37.0	34.0	39.0	28.0	39.0
10	34.531663564813776	37.0	32.0	39.0	28.0	39.0
11	34.857994932394554	37.0	34.0	39.0	28.0	39.0
12	34.87675852635827	37.0	34.0	39.0	27.0	39.0
13	34.825858241585756	37.0	33.0	39.0	27.0	39.0
14	35.66391012848398	38.0	34.0	40.0	27.0	41.0
15	35.70735251250084	38.0	33.0	40.0	27.0	41.0
16	36.00860596004215	37.0	34.0	40.0	30.0	41.0
17	35.04742023006032	37.0	32.0	40.0	27.0	41.0
18	34.98003901607731	37.0	33.0	39.0	27.0	40.0
19	34.36106913021055	37.0	32.0	38.0	27.0	40.0
20	33.1215104154988	35.0	31.0	37.0	25.0	39.0
21	34.10128483978743	35.0	33.0	38.0	27.0	40.0
22	34.68432854227863	35.0	33.0	39.0	28.0	40.0
23	34.74687983496648	35.0	33.0	39.0	28.0	40.0
24	34.52191403009171	35.0	33.0	39.0	27.0	40.0
25	33.6033320626948	35.0	33.0	38.0	24.0	40.0
26	33.6167724286387	35.0	32.0	38.0	24.0	40.0
27	34.26258268493397	35.0	33.0	38.0	27.0	40.0
28	33.73574904141534	35.0	33.0	38.0	24.0	40.0
29	33.66437204296253	35.0	33.0	39.0	24.0	40.0
30	33.13980761037738	35.0	32.0	38.0	21.0	40.0
31	33.350445097203846	35.0	33.0	39.0	22.0	40.0
32	32.751207480323785	35.0	32.0	39.0	18.0	40.0
33	31.800228714935983	35.0	30.0	39.0	15.0	40.0
34	31.540704531694956	35.0	30.0	39.0	10.0	40.0
35	30.996322622597933	35.0	28.0	39.0	8.0	40.0
36	30.556606946655606	35.0	25.0	39.0	8.0	40.0
37	30.557902997959506	35.0	25.0	39.0	7.0	40.0
38	30.035289369240083	35.0	23.0	39.0	8.0	40.0
39	29.871202098795884	35.0	23.0	39.0	7.0	40.0
40	29.30692647487499	35.0	21.0	39.0	7.0	40.0
41	28.697854115747695	34.0	21.0	38.0	7.0	40.0
42	29.510034307240396	35.0	22.0	39.0	7.0	40.0
43	29.20561921205462	35.0	21.0	39.0	7.0	40.0
44	29.589393905419648	35.0	22.0	39.0	7.0	40.0
45	29.719528219386955	35.0	23.0	39.0	7.0	40.0
46	29.396300199564994	35.0	22.0	39.0	7.0	40.0
47	29.106177545574816	35.0	21.0	39.0	7.0	40.0
48	29.00350247774514	34.0	20.0	38.0	7.0	40.0
49	29.336466578469405	35.0	21.0	39.0	7.0	40.0
50	29.112738525012894	35.0	20.0	39.0	7.0	40.0
51	28.03591721416239	33.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	11.0
11	14.0
12	18.0
13	23.0
14	33.0
15	63.0
16	124.0
17	248.0
18	527.0
19	949.0
20	1483.0
21	2321.0
22	2938.0
23	4223.0
24	5983.0
25	8318.0
26	11011.0
27	11574.0
28	10812.0
29	10360.0
30	11097.0
31	12486.0
32	14502.0
33	16787.0
34	18786.0
35	20842.0
36	21062.0
37	19698.0
38	14425.0
39	2255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.96226203556293	2.1517142408682197	24.85548355270534	34.03054017086351
2	33.36053994663318	29.07639527322466	26.394600533668182	11.168464246473977
3	9.808731529026616	29.323048635558447	49.61499652442989	11.253223310985044
4	9.229768818530394	2.461600556091217	75.09428885351032	13.214341771868062
5	30.564836199744377	2.8122967912639867	50.08991636208714	16.532950646904503
6	11.037513734107675	25.19810749601991	54.7377626297733	9.02661614009911
7	43.93120613494182	1.0556763907886182	51.8106599098594	3.202457564410162
8	40.887055183084065	20.74579007556562	33.80765522344552	4.559499517904792
9	38.760006278449225	2.5436688566495507	31.794066865484226	26.902257999417
10	30.541516245487365	15.796129784514653	38.772114716236516	14.890239253761465
11	27.308114895620783	14.05610242841447	44.88014888893872	13.755633787026033
12	18.8165123214566	12.110231629930263	46.2111801242236	22.862075924389533
13	17.69760297777877	14.966477565755545	58.050989976904276	9.284929479561406
14	15.539161827028725	24.14646725116039	41.90909702446353	18.405273897347353
15	9.010023095723927	14.531919187389287	60.582101935107744	15.875955781779044
16	10.71641590241496	17.45364037939772	43.56884992264053	28.26109379554679
17	11.241114873197748	18.575688947687063	53.198197188151674	16.98499899096352
18	10.786375765186	16.820862389846848	50.52223243715945	21.8705294078077
19	11.48642285355517	20.780321546292353	44.863555844563535	22.86969975558894
20	15.06199968607754	17.491759535394756	55.69657151826356	11.749669260264143
21	15.12747494226069	28.09202412718344	44.351413772226834	12.429087158329036
22	12.016503352243424	14.40186559634056	49.87465524586855	23.706975805547458
23	12.90938852389174	26.899567235464268	46.43316815032401	13.757876090319975
24	15.86339888333296	16.988138215575038	44.0626051079669	23.085857793125097
25	9.926676682288047	31.8904859071238	41.81940489270578	16.363432517882366
26	12.523712357333453	15.223893983900263	50.93481624324506	21.317577415521225
27	16.785433997802542	20.910823597999865	42.68583088548557	19.617911518712024
28	10.35540507209005	19.327309011816936	52.56541919860081	17.751866717492206
29	22.10731663564814	13.766845303495751	43.93613920218849	20.189698858667622
30	11.680606318810682	25.464941587999192	48.995672354642686	13.858779738547437
31	18.01197389958966	16.237415072762744	38.4312846155571	27.3193264120905
32	20.441285288248086	23.152229970625825	38.005695450366616	18.400789290759466
33	13.636791712447025	21.63194833733211	37.22537390407427	27.5058860461466
34	25.079713882099693	22.97777877435702	34.34356571069802	17.598941632845257
35	13.326905397224028	19.87846716146826	41.37498037984618	25.419647061461532
36	20.623360315716305	20.27445792317869	35.51494495145413	23.587236809650875
37	13.58252797273359	20.311231697199364	43.63208287552974	22.4741574545373
38	15.544543354934188	26.64215081731955	31.540238132609815	26.273067695136444
39	18.404825436688565	26.904051842052155	33.73096845079265	20.960154270466624
40	14.746731842949076	24.141534183913716	35.97058098078346	25.141152992353742
41	15.17635715406866	28.784895845012	36.82758929972868	19.21115770119066
42	14.407247124246025	20.18028118483306	40.42379532255533	24.988676368365585
43	20.247998744310156	20.636365674821178	36.17238827723838	22.94324730363029
44	19.938560889745947	21.230576047716216	37.85097652308451	20.979886539453325
45	15.19743480503173	19.69056214543579	35.116263425790976	29.995739623741507
46	24.710182299257795	24.79314752113371	30.903423997129853	19.59324618247864
47	13.491041998340695	20.374016189429785	46.62107316635648	19.513868645873043
48	20.560575823485884	25.129941475884028	30.515505527277618	23.793977173352467
49	15.72482453976725	18.050541516245488	41.06913021055228	25.155503733434987
50	19.93766396842837	17.982375496109604	37.83752270332085	24.242437832141174
51	19.538533982106422	18.76404242437832	33.26277552301724	28.434648070498014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	278.0
2	544.0
3	6115.0
4	11686.0
5	8946.0
6	6206.0
7	6124.0
8	6042.0
9	5984.0
10	5926.0
11	5692.0
12	5458.0
13	5098.5
14	4739.0
15	4362.5
16	3986.0
17	3635.0
18	3284.0
19	3083.0
20	2882.0
21	2649.5
22	2417.0
23	2352.0
24	2287.0
25	2261.0
26	2401.0
27	2567.0
28	2617.0
29	2667.0
30	2661.0
31	2655.0
32	3047.5
33	3440.0
34	3631.5
35	3823.0
36	4013.0
37	4203.0
38	4682.5
39	5162.0
40	5460.0
41	5758.0
42	6560.5
43	7363.0
44	8206.5
45	9050.0
46	30270.5
47	51491.0
48	35150.0
49	18809.0
50	16026.0
51	13243.0
52	10669.5
53	8096.0
54	7272.0
55	6448.0
56	5955.5
57	5463.0
58	5007.0
59	4551.0
60	3939.5
61	3328.0
62	2879.5
63	2431.0
64	2039.0
65	1647.0
66	1343.5
67	1040.0
68	897.5
69	755.0
70	616.0
71	477.0
72	402.5
73	328.0
74	268.5
75	183.5
76	158.0
77	109.0
78	60.0
79	42.0
80	24.0
81	24.0
82	24.0
83	14.0
84	4.0
85	4.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	222985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.802296118573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55023465922204	35.31448303697558
2	4.782212061593924	3.902504652779335
3	1.6563533846982403	2.027490638383748
4	0.9705109745776683	1.583963046841716
5	0.7484914764296626	1.5270085431755498
6	0.5924183638701735	1.450321770522681
7	0.49130057263444815	1.4032334013498666
8	0.46162469911961573	1.5068278135300581
9	0.39567831353109917	1.453012534475413
>10	3.2357693195432113	22.295670112339394
>50	0.061549959882615426	1.8207502746821536
>100	0.03956783135310992	2.9504226741709085
>500	0.003297319279425827	1.032804897190394
>1k	0.006594638558851654	5.60127362827096
>5k	0.003297319279425827	10.69219902684037
>10k+	0.0010991064264752755	5.43803394847187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	12126	5.43803394847187	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	9707	4.3532076148619865	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	8667	3.8868085297217303	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5468	2.452182882256654	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT	2481	1.1126308944547838	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC	2153	0.9655357983720879	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA	2079	0.9323497096217234	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	2016	0.9040966881180348	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	1941	0.8704621387088817	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	1820	0.8161983989954481	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGC	997	0.44711527681234164	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGT	757	0.33948471870305175	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT	549	0.24620490167500056	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	445	0.19956499316097492	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATG	382	0.17131197165728637	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCG	371	0.1663789044106106	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCCAACT	336	0.1506827813530058	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTCCAACTT	280	0.12556898446083817	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	255	0.11435746799112048	No Hit
GTTCTGGGTCACTGAAGGTGACTACTTTTTGCTTGAATGAATTTCCTAATC	249	0.11166670403838823	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12915666973114784	0.0
2	0.0	0.0	0.0	1.218467609928919	0.0
3	0.0	0.0	0.0	1.354799650200686	0.0
4	0.0	0.0	0.0	2.3683207390631655	0.0
5	0.0	0.0	0.0	6.9619032670359	0.0
6	0.0	0.0	0.0	7.283898020046191	0.0
7	0.0	0.0	0.0	8.375899724196694	0.0
8	0.0	0.0	0.0	9.576428907774066	0.0
9	0.0	0.0	0.0	9.757158553265915	0.0
10	0.0	0.0	0.0	16.44191313317039	0.0
11	0.0	0.0	0.0	16.71278337107877	0.0
12	0.0	0.0	0.0	22.535596564791355	0.0
13	0.0	0.0	0.0	22.906473529609617	0.0
14	0.0	0.0	0.0	23.264345135323005	0.0
15	0.0	0.0	0.0	24.523174204542908	0.0
16	0.0	0.0	0.0	25.0595331524542	0.0
17	0.0	0.0	0.0	25.298114222929794	0.0
18	0.0	0.0	0.0	25.470771576563447	0.0
19	0.0	0.0	0.0	27.033656972442092	0.0
20	0.0	0.0	0.0	27.333677153171738	0.0
21	0.0	0.0	0.0	27.494674529676885	0.0
22	0.0	0.0	0.0	27.891113752046103	0.0
23	0.0	0.0	0.0	28.03462116285849	0.0
24	0.0	0.0	0.0	28.15839630468417	0.0
25	0.0	0.0	0.0	28.254366885664954	0.0
26	0.0	0.0	0.0	28.38531739803126	0.0
27	0.0	0.0	0.0	28.612238491378342	0.0
28	0.0	0.0	0.0	28.71403906092338	0.0
29	0.0	0.0	0.0	28.842298809336953	0.0
30	0.0	0.0	0.0	28.976837006973565	0.0
31	0.0	0.0	0.0	29.113169047245332	0.0
32	0.0	0.0	0.0	29.254434154763775	0.0
33	0.0	0.0	0.0	29.38090006054219	0.0
34	0.0	0.0	0.0	29.61589344574747	0.0
35	0.0	0.0	0.0	29.736529362961633	0.0
36	0.0	0.0	0.0	29.86299526874005	0.0
37	0.0	0.0	0.0	30.01861111733973	0.0
38	0.0	0.0	0.0	30.17243312330426	0.0
39	0.0	0.0	0.0	30.326703589927572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	30	2.157607E-6	45.000004	17
GCGATCT	30	2.157607E-6	45.000004	9
TCACGGG	20	7.0210453E-4	45.0	3
TGATCCG	25	3.880662E-5	45.0	4
GCGACCG	20	7.0210453E-4	45.0	11
AGTCATG	25	3.880662E-5	45.0	26
CTCGTCC	20	7.0210453E-4	45.0	37
TGCTTCG	35	1.2060445E-7	45.0	1
CACGGGG	20	7.0210453E-4	45.0	4
CATTGGG	20	7.0210453E-4	45.0	3
GCTATAA	20	7.0210453E-4	45.0	33
ACCTGGG	20	7.0210453E-4	45.0	3
CCCAGCG	20	7.0210453E-4	45.0	29
GTTAGTC	20	7.0210453E-4	45.0	35
TTCAAGT	20	7.0210453E-4	45.0	22
TACGGTG	35	1.2060445E-7	45.0	7
CCTCGTC	20	7.0210453E-4	45.0	36
TTTCACG	20	7.0210453E-4	45.0	1
TGCATCC	20	7.0210453E-4	45.0	44
CTACGGC	25	3.880662E-5	45.0	6
>>END_MODULE