FastQCFastQC Report
Sat 14 Jan 2017
SRR2933465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141784
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75245.306663657394346No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG58634.135163347063138No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC41192.9051232861253737No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC27461.9367488574169158No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT12300.867516786097162No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC11820.8336624724933702No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT7930.5593014726626417No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA7710.5437849122609039No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGC7600.536026632060035No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC7090.5000564238560063No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT5850.4125994470462111No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTCCAACTTCGTATGCCG4820.3399537324380748No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT3660.2581391412289116No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGT3010.21229475822377702No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCG2930.20665237262314506No Hit
GAATCTTTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC2630.18549342662077525No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT2450.17279805901935338No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT2370.16715567341872142No Hit
TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC2090.14740732381650962No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTCCAACTTCGTATGCCGTCTTC1970.1389437454155617No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC1950.13753314901540373No Hit
GCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCGTCTTCTGC1700.11990069401342889No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCCAACT1690.1191953958133499No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG1680.1184900976132709No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC1660.1170795012131129No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGTCCAACTTCGTATGC1550.10932122101224397No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT1540.10861592281216498No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATG1500.105794730011849No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG1460.10297353721153304No Hit
GAATCTGTCTCTTATCCCCATCTGACGCGTCCAACTTCGTATGCCGTCTTC1430.10085764261129607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG551.8189894E-1245.00000417
GCGACCT600.045.00000411
ACGGCGC600.045.0000048
TATTATA302.15201E-645.00000424
GCAGGGC302.15201E-645.0000044
GCATAAT253.8734655E-545.010
TGATCCG502.1827873E-1145.04
CCGGGGC207.012354E-445.05
GCCCAAG207.012354E-445.038
GCGACCG453.8016879E-1045.011
AATTTAC207.012354E-445.014
TACTTCC207.012354E-445.025
CCGATGA351.201879E-745.018
CATGCAC253.8734655E-545.045
TTGCTCG207.012354E-445.01
ACCGCCT351.201879E-745.014
TTTCGCG207.012354E-445.01
CGACCGC351.201879E-745.012
GGTCACT207.012354E-445.07
GACCCGT351.201879E-745.038
Produced by FastQC (version 0.11.5)