##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933465.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141784
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.319373130959768	30.0	25.0	31.0	16.0	33.0
2	26.905588782937425	31.0	16.0	31.0	16.0	34.0
3	27.08062263725103	31.0	16.0	33.0	16.0	34.0
4	30.848092873666985	35.0	28.0	35.0	19.0	37.0
5	33.39740732381651	35.0	32.0	37.0	28.0	37.0
6	31.016990633639903	35.0	28.0	37.0	17.0	37.0
7	32.12047198555549	33.0	32.0	35.0	26.0	37.0
8	31.664172262032388	35.0	31.0	35.0	17.0	37.0
9	32.87112791288157	34.0	32.0	37.0	25.0	39.0
10	31.723240986289003	35.0	28.0	39.0	17.0	39.0
11	32.36173334085652	35.0	28.0	39.0	17.0	39.0
12	33.16688766010269	35.0	31.0	39.0	23.0	39.0
13	33.25813208824691	35.0	31.0	39.0	25.0	39.0
14	33.35302290808554	36.0	31.0	39.0	19.0	41.0
15	33.665561699486545	36.0	31.0	39.0	23.0	41.0
16	34.01018450600914	36.0	32.0	40.0	25.0	41.0
17	31.769148846132143	34.0	27.0	39.0	18.0	41.0
18	32.662035208486145	35.0	30.0	39.0	23.0	39.0
19	32.41738842182475	35.0	30.0	37.0	19.0	39.0
20	31.694690515149805	34.0	29.0	36.0	21.0	39.0
21	32.475857642611295	35.0	31.0	37.0	23.0	39.0
22	32.92483637081758	34.0	31.0	37.0	25.0	39.0
23	33.56765925633358	35.0	32.0	38.0	26.0	40.0
24	32.41316368560627	35.0	31.0	37.0	22.0	39.0
25	31.52962252440332	35.0	30.0	37.0	18.0	39.0
26	30.872940529255768	33.0	26.0	37.0	19.0	39.0
27	32.56544461998533	35.0	31.0	37.0	23.0	39.0
28	32.25432347796649	35.0	30.0	37.0	21.0	39.0
29	32.326313265248544	35.0	30.0	38.0	21.0	40.0
30	31.319507137617784	34.0	29.0	37.0	18.0	40.0
31	31.782909214015685	35.0	30.0	37.0	20.0	39.0
32	31.767872256390003	35.0	30.0	37.0	18.0	39.0
33	31.41053292331998	35.0	30.0	38.0	15.0	40.0
34	30.82276561530215	35.0	29.0	38.0	12.0	40.0
35	30.394346329628167	35.0	27.0	38.0	10.0	40.0
36	29.781209445353497	35.0	25.0	38.0	8.0	40.0
37	29.898359476386617	35.0	25.0	38.0	8.0	40.0
38	29.318011905433618	34.0	23.0	38.0	8.0	40.0
39	29.149629013146757	34.0	23.0	37.0	8.0	40.0
40	28.525588218698864	33.0	22.0	37.0	7.0	39.0
41	27.628089206116346	33.0	19.0	37.0	7.0	39.0
42	28.840510918016136	33.0	23.0	37.0	7.0	40.0
43	28.54981521187158	34.0	21.0	37.0	7.0	40.0
44	28.768358912148056	34.0	22.0	38.0	7.0	40.0
45	29.155948485019465	34.0	23.0	38.0	7.0	40.0
46	28.767025898549907	34.0	22.0	38.0	7.0	40.0
47	28.61392681825876	34.0	22.0	38.0	7.0	40.0
48	28.514169440839588	34.0	22.0	37.0	7.0	40.0
49	28.659954578795915	34.0	22.0	37.0	7.0	40.0
50	28.58890989110196	34.0	21.0	38.0	7.0	40.0
51	26.913706765220336	31.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	15.0
12	15.0
13	9.0
14	27.0
15	74.0
16	186.0
17	313.0
18	525.0
19	862.0
20	1310.0
21	1839.0
22	2534.0
23	3453.0
24	4613.0
25	6126.0
26	7800.0
27	8485.0
28	8556.0
29	8807.0
30	9801.0
31	11106.0
32	11797.0
33	12509.0
34	13304.0
35	12985.0
36	9037.0
37	4685.0
38	979.0
39	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.24363821023529	2.2379111888506458	27.999633244935957	35.51881735597811
2	36.36023810867235	23.406731366021553	28.099785589347174	12.133244935958922
3	10.745923376403542	23.300231337809628	53.89395136263613	12.059893923150709
4	10.158409975737742	2.539073520284376	73.13237036619083	14.170146137787057
5	27.097556847034927	2.8755007617220563	53.95037521864244	16.076567172600576
6	11.522456694690515	20.27661795407098	57.00925351238504	11.191671838853468
7	44.50149523218417	1.053715510918016	51.050189019917624	3.394600236980195
8	43.31588895785138	17.015319076905715	34.10116797381933	5.567623991423574
9	40.1187722168933	2.5150933814816905	33.654008914969246	23.712125486655758
10	28.173841900355473	16.651385205664955	39.60460982903572	15.57016306494386
11	24.969672177396603	15.36421599052079	46.58494611521751	13.08116571686509
12	18.775038086102803	13.029678948259324	47.557552333126445	20.637730632511424
13	17.099954860915194	15.899537324380747	57.64261129605597	9.357896518648085
14	14.866275461265023	21.419201038198953	44.125571291542066	19.588952208993963
15	8.99678384020764	15.620944535349546	59.46298595045986	15.91928567398296
16	10.80516842521018	15.56945776674378	45.486091519494444	28.1392822885516
17	11.0294532528353	17.67618349037973	53.80296789482594	17.491395361959036
18	10.586525983185691	15.510918016137223	52.326073463860524	21.576482536816567
19	12.147350899960504	19.87812447102635	46.12579698696609	21.84872764204706
20	14.402189245613044	16.778338881679176	56.545167296733055	12.274304575974721
21	15.725328668961236	25.53179484285956	45.18422388986063	13.558652598318568
22	11.990069401342888	15.166732494498675	50.11355301021272	22.72964509394572
23	12.418185408790837	25.710940585679626	46.438244089601085	15.432629915928455
24	14.963606612875925	16.034954578795915	46.24358178637928	22.75785702194888
25	10.525870337978898	27.264007222253568	43.753173841900356	18.45694859786718
26	12.45838740619534	16.183067200812502	51.42399706595949	19.93454832703267
27	14.017801726569996	20.48185973029397	45.605992213507875	19.894346329628167
28	10.17392653613948	18.322941939852168	53.60336850420357	17.899763019804773
29	19.670061502003048	13.899311628956724	45.798538622129435	20.632088246910794
30	11.709360717711448	24.219234892512553	49.38709586413135	14.684308525644644
31	16.74095807707499	17.385600631947188	40.735202843762345	25.138238447215482
32	18.23830615584269	22.972972972972975	40.72532866896124	18.0633922022231
33	12.936579585848898	20.320346442475877	40.40794447892569	26.335129492749537
34	22.957456412571233	22.06595948767139	36.975963437341306	18.00062066241607
35	12.59239406421035	19.66935620380297	43.229842577441744	24.50840715454494
36	19.695452237205892	18.852620888111492	37.438639056593125	24.013287818089488
37	12.921063025447161	20.06009140664673	44.46481972578006	22.55402584212605
38	15.09972916549117	25.800513457089657	34.588529030073914	24.511228347345256
39	16.74518986627546	26.445156011961856	36.17403938385149	20.63561473791119
40	14.88249731986684	23.19161541499746	36.90331772273317	25.02256954240253
41	14.835947638661626	26.027619477515096	39.206821644191166	19.929611239632116
42	14.156040173785476	19.342097838966314	41.303673193026015	25.198188794222197
43	19.225018337753202	19.41615414997461	38.36116910229645	22.997658409975738
44	19.26945212435818	20.43954183828923	39.57639790103256	20.714608136320038
45	15.007335101280821	19.292726964960785	36.70795012131129	28.9919878124471
46	22.10545618687581	23.964622242284037	33.57360492016024	20.356316650679908
47	13.745556621339503	19.078316312136774	46.793009084240815	20.383117982282908
48	19.304717034362127	23.882102352874796	33.74992947017999	23.063251142583084
49	15.434040512328611	17.15285222592112	41.348812277831065	26.0642949839192
50	19.040935507532588	17.664898719178467	38.462026744907746	24.8321390283812
51	18.54087908367658	18.47669694746939	35.247982847147775	27.734441121706254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	96.5
2	176.0
3	5543.0
4	10910.0
5	7430.5
6	3951.0
7	3890.5
8	3830.0
9	3829.5
10	3829.0
11	3651.5
12	3474.0
13	3201.5
14	2929.0
15	2674.5
16	2420.0
17	2196.5
18	1973.0
19	1785.5
20	1598.0
21	1543.5
22	1489.0
23	1422.5
24	1356.0
25	1338.0
26	1302.0
27	1284.0
28	1375.0
29	1466.0
30	1505.0
31	1544.0
32	1780.0
33	2016.0
34	2131.5
35	2247.0
36	2359.5
37	2472.0
38	2773.0
39	3074.0
40	3355.0
41	3636.0
42	4238.5
43	4841.0
44	5475.5
45	6110.0
46	14926.0
47	23742.0
48	19365.0
49	14988.0
50	13043.0
51	11098.0
52	8776.5
53	6455.0
54	5517.0
55	4579.0
56	4094.5
57	3610.0
58	3154.0
59	2698.0
60	2389.5
61	2081.0
62	1788.5
63	1496.0
64	1285.0
65	1074.0
66	874.5
67	675.0
68	567.5
69	460.0
70	392.0
71	324.0
72	272.5
73	221.0
74	185.5
75	114.5
76	79.0
77	65.5
78	52.0
79	38.0
80	24.0
81	18.0
82	12.0
83	7.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	141784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.688399255205105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.16376004166601	37.611437115612475
2	5.9768627389087925	5.341928567398296
3	2.2947870140938433	3.0765107487445693
4	1.4362147062072883	2.567285448287536
5	1.0227111314531019	2.2851661682559388
6	0.8743548870756459	2.344411217062574
7	0.714950837265826	2.2365005924504877
8	0.5760641404018244	2.059470744230661
9	0.4340209277000047	1.7456130451955088
>10	2.381591199633844	16.528663318851212
>50	0.06470857467527344	1.9776561530214973
>100	0.04261296381054592	3.688709586413135
>500	0.00789128959454554	2.551768887885798
>1k	0.006313031675636433	6.543051402132821
>5k	0.0031565158378182166	9.441827004457485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7524	5.306663657394346	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	5863	4.135163347063138	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	4119	2.9051232861253737	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	2746	1.9367488574169158	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT	1230	0.867516786097162	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	1182	0.8336624724933702	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	793	0.5593014726626417	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA	771	0.5437849122609039	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGC	760	0.536026632060035	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC	709	0.5000564238560063	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	585	0.4125994470462111	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTCCAACTTCGTATGCCG	482	0.3399537324380748	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	366	0.2581391412289116	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGT	301	0.21229475822377702	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCG	293	0.20665237262314506	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	263	0.18549342662077525	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	245	0.17279805901935338	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTAT	237	0.16715567341872142	No Hit
TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	209	0.14740732381650962	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	197	0.1389437454155617	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	195	0.13753314901540373	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCCACTTCGTATGCCGTCTTCTGC	170	0.11990069401342889	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCCAACT	169	0.1191953958133499	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	168	0.1184900976132709	No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	166	0.1170795012131129	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGTCCAACTTCGTATGC	155	0.10932122101224397	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	154	0.10861592281216498	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATG	150	0.105794730011849	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	146	0.10297353721153304	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCGTCCAACTTCGTATGCCGTCTTC	143	0.10085764261129607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31315240083507306	0.0
2	0.0	0.0	0.0	1.802742199401907	0.0
3	0.0	0.0	0.0	2.0961462506347686	0.0
4	0.0	0.0	0.0	3.1858319697568134	0.0
5	0.0	0.0	0.0	5.94848501946623	0.0
6	0.0	0.0	0.0	6.365316255712916	0.0
7	0.0	0.0	0.0	7.086131016193646	0.0
8	0.0	0.0	0.0	8.003723974496417	0.0
9	0.0	0.0	0.0	8.226598205721379	0.0
10	0.0	0.0	0.0	14.065762004175365	0.0
11	0.0	0.0	0.0	14.866275461265023	0.0
12	0.0	0.0	0.0	19.679230378604075	0.0
13	0.0	0.0	0.0	20.083366247249337	0.0
14	0.0	0.0	0.0	20.383117982282908	0.0
15	0.0	0.0	0.0	21.296479151385206	0.0
16	0.0	0.0	0.0	21.844495852846585	0.0
17	0.0	0.0	0.0	22.28178073689556	0.0
18	0.0	0.0	0.0	22.66687355413869	0.0
19	0.0	0.0	0.0	24.188907069909156	0.0
20	0.0	0.0	0.0	24.647350899960504	0.0
21	0.0	0.0	0.0	25.038791401004346	0.0
22	0.0	0.0	0.0	25.82660949049258	0.0
23	0.0	0.0	0.0	26.230745359137842	0.0
24	0.0	0.0	0.0	26.487473903966595	0.0
25	0.0	0.0	0.0	26.725864695593298	0.0
26	0.0	0.0	0.0	26.91135812221407	0.0
27	0.0	0.0	0.0	27.156096597641483	0.0
28	0.0	0.0	0.0	27.379676127066524	0.0
29	0.0	0.0	0.0	27.590560288890142	0.0
30	0.0	0.0	0.0	27.850815324719292	0.0
31	0.0	0.0	0.0	28.084974327145517	0.0
32	0.0	0.0	0.0	28.297974383569372	0.0
33	0.0	0.0	0.0	28.525785702194888	0.0
34	0.0	0.0	0.0	28.758534108220957	0.0
35	0.0	0.0	0.0	28.96589177904418	0.0
36	0.0	0.0	0.0	29.170428257067087	0.0
37	0.0	0.0	0.0	29.37849122609039	0.0
38	0.0	0.0	0.0	29.608418439316143	0.0
39	0.0	0.0	0.0	29.81366021553913	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	55	1.8189894E-12	45.000004	17
GCGACCT	60	0.0	45.000004	11
ACGGCGC	60	0.0	45.000004	8
TATTATA	30	2.15201E-6	45.000004	24
GCAGGGC	30	2.15201E-6	45.000004	4
GCATAAT	25	3.8734655E-5	45.0	10
TGATCCG	50	2.1827873E-11	45.0	4
CCGGGGC	20	7.012354E-4	45.0	5
GCCCAAG	20	7.012354E-4	45.0	38
GCGACCG	45	3.8016879E-10	45.0	11
AATTTAC	20	7.012354E-4	45.0	14
TACTTCC	20	7.012354E-4	45.0	25
CCGATGA	35	1.201879E-7	45.0	18
CATGCAC	25	3.8734655E-5	45.0	45
TTGCTCG	20	7.012354E-4	45.0	1
ACCGCCT	35	1.201879E-7	45.0	14
TTTCGCG	20	7.012354E-4	45.0	1
CGACCGC	35	1.201879E-7	45.0	12
GGTCACT	20	7.012354E-4	45.0	7
GACCCGT	35	1.201879E-7	45.0	38
>>END_MODULE