##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933464.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408814
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.37367115607587	31.0	28.0	31.0	25.0	34.0
2	28.49631861922537	31.0	26.0	33.0	16.0	34.0
3	28.55550201314045	31.0	26.0	34.0	16.0	34.0
4	33.02770453066676	35.0	32.0	37.0	28.0	37.0
5	34.163984110133214	35.0	33.0	37.0	30.0	37.0
6	33.4694677775223	35.0	32.0	37.0	28.0	37.0
7	33.388472508280046	35.0	32.0	36.0	28.0	37.0
8	33.332366308394526	35.0	33.0	37.0	28.0	37.0
9	34.56605448932766	37.0	34.0	39.0	28.0	39.0
10	34.31357047459236	37.0	32.0	39.0	27.0	39.0
11	34.45899357654092	37.0	32.0	39.0	27.0	39.0
12	34.576751285425644	37.0	33.0	39.0	27.0	39.0
13	34.596554912502995	37.0	33.0	39.0	27.0	39.0
14	35.51455429608575	38.0	34.0	40.0	27.0	41.0
15	35.61805857920717	38.0	33.0	40.0	27.0	41.0
16	35.70816557162915	37.0	34.0	40.0	27.0	41.0
17	35.009111723179736	37.0	33.0	40.0	27.0	41.0
18	34.93050873991595	37.0	33.0	39.0	27.0	40.0
19	34.32183829320914	37.0	32.0	38.0	27.0	40.0
20	33.705039455595944	35.0	32.0	38.0	25.0	40.0
21	34.43611520153419	35.0	33.0	39.0	27.0	40.0
22	35.10778495844076	36.0	33.0	39.0	30.0	40.0
23	35.36260499885033	36.0	34.0	39.0	30.0	40.0
24	35.0085026442343	36.0	34.0	39.0	29.0	40.0
25	34.017746456823886	35.0	33.0	39.0	25.0	40.0
26	34.12443067018253	35.0	33.0	39.0	25.0	40.0
27	34.7058833601589	36.0	33.0	39.0	27.0	40.0
28	34.470167362174486	35.0	33.0	39.0	25.0	40.0
29	34.60157186397726	36.0	33.0	39.0	25.0	40.0
30	33.978491441095464	35.0	33.0	39.0	24.0	40.0
31	34.01372751422407	35.0	33.0	39.0	24.0	40.0
32	33.60211245211759	36.0	33.0	39.0	21.0	40.0
33	33.28773231836483	36.0	33.0	39.0	18.0	41.0
34	32.93115695646431	36.0	32.0	40.0	15.0	41.0
35	32.61977329543509	36.0	32.0	40.0	12.0	41.0
36	32.18990298766676	36.0	31.0	40.0	10.0	41.0
37	32.07472102227419	36.0	31.0	40.0	10.0	41.0
38	31.909022195913057	36.0	30.0	40.0	10.0	41.0
39	31.591197953103368	36.0	30.0	39.0	10.0	40.0
40	31.16725454607719	35.0	29.0	39.0	8.0	40.0
41	30.705575151535907	35.0	26.0	39.0	8.0	40.0
42	30.98976796293669	35.0	28.0	39.0	8.0	40.0
43	30.90181598477547	35.0	27.0	39.0	8.0	40.0
44	31.13557265651372	35.0	29.0	39.0	8.0	40.0
45	31.355207991898517	36.0	29.0	39.0	8.0	40.0
46	31.14271526904656	35.0	29.0	39.0	8.0	40.0
47	30.953783383152242	35.0	28.0	39.0	8.0	40.0
48	30.818095759929943	35.0	28.0	39.0	8.0	40.0
49	31.026713860092855	35.0	29.0	39.0	8.0	40.0
50	30.894648911240807	35.0	28.0	39.0	8.0	40.0
51	29.36383538724212	34.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	14.0
10	29.0
11	32.0
12	31.0
13	39.0
14	67.0
15	93.0
16	175.0
17	364.0
18	699.0
19	1286.0
20	1911.0
21	2933.0
22	4064.0
23	5853.0
24	8136.0
25	11987.0
26	15565.0
27	17013.0
28	16820.0
29	16919.0
30	18402.0
31	21699.0
32	25843.0
33	30565.0
34	34660.0
35	40123.0
36	44698.0
37	46750.0
38	36457.0
39	5574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.00865430244561	2.549570220197938	33.40516714202547	31.036608335330985
2	31.102897650276162	20.769836649429816	33.6407265895982	14.486539110695817
3	13.879661655422758	20.891163218480777	51.34217517012627	13.886999955970197
4	12.303639307851492	3.200966698792116	67.83916402080163	16.656229972554755
5	25.986634508602936	3.708532486656523	51.21228725043663	19.092545754303913
6	14.995083338633217	18.407637703209772	55.05706751725724	11.540211440899775
7	56.06168086220139	1.4192273258743586	39.578390172547905	2.9407016393763423
8	54.4059156486813	14.253425763305563	27.527922233582995	3.8127363544301325
9	50.97941851306462	3.25771622302563	26.517682858219143	19.24518240569061
10	33.92863258107599	18.54486392344685	33.44699545514586	14.079508040331302
11	29.405548733653937	16.01755321490947	39.56395818147128	15.012939869965313
12	21.865689531180436	14.953744245549322	42.40314666327475	20.7774195599955
13	21.081469812677646	15.343652614636486	50.95887127153179	12.61600630115407
14	18.209748198447215	22.200560646161822	41.41590062962619	18.173790525764772
15	13.322440033854027	15.657976488085046	53.68920829521494	17.330375182845987
16	16.44513152680681	18.44873218627542	40.071768579353936	25.03436770756383
17	17.619748834433263	18.6676581526073	46.37757023976674	17.335022773192698
18	17.374404986130614	17.362663705254715	44.21521767845524	21.047713630159436
19	16.976669096459513	20.09006540871888	41.21140665437093	21.72185884045067
20	18.37045698043609	20.52473741114541	47.49079043281297	13.614015175605532
21	19.041666870508347	25.25011374365849	41.67518724896897	14.033032136864199
22	16.391561932810518	15.637184636533974	46.26260353119022	21.708649899465282
23	16.934840783339123	24.06791352546635	43.76537985489733	15.231865836297192
24	19.0766461031178	19.038976160307623	40.13316569393416	21.751212042640418
25	16.48622600987246	28.616681424804437	37.72375701419227	17.173335551130833
26	16.239414501460324	16.659165292773732	43.95593105911246	23.14548914665349
27	19.340335702789044	21.030346318863835	39.493021276179384	20.136296702167733
28	13.97897332283141	20.391424951200303	45.91599113533294	19.71361059063535
29	19.219742963792825	18.26772077277197	42.13407564320204	20.378460620233163
30	17.28610076954312	26.34816811557334	41.580278561888775	14.78545255299476
31	24.533651000210366	17.047606001751408	37.648661738590164	20.770081259448062
32	21.906050184191344	24.388841869407603	35.75538019735136	17.94972774904969
33	20.206499777404883	22.160933823205664	33.71704491529155	23.9155214840979
34	24.63320727763726	20.742929547422545	38.68311750576056	15.940745669179627
35	17.73471554300978	29.531767503069855	32.7119423503109	20.021574603609466
36	26.626045096302963	22.65431223001169	34.094967393484566	16.624675280200776
37	18.436746295381273	27.35963054102844	33.986849765418995	20.216773398171295
38	25.000122305009125	26.89658377648515	29.111038271683455	18.99225564682227
39	25.42721139686997	21.427592988498436	31.14081220310459	22.004383411527005
40	19.88385916333589	29.304035576081056	33.14221137240897	17.669893888174084
41	27.197209488911827	23.9098954536782	29.31553224693871	19.577362810471264
42	18.341103778246342	25.943827755409547	36.73700020058022	18.97806826576389
43	24.992784004461686	21.22750199357165	32.02189748883355	21.757816513133115
44	22.61615306716502	22.331182395906207	33.67888575244488	21.373778784483896
45	19.96947266972266	20.715777835397027	32.758173643759754	26.556575851120556
46	25.36361279212551	23.942673196123422	30.298130690240548	20.395583321510514
47	16.23378847104062	23.057184930065997	41.899250025684054	18.809776573209334
48	21.504645144246528	25.69359170674194	30.323080912101837	22.478682236909695
49	18.71095412583718	21.729441751016353	38.67186544492116	20.887738678225308
50	21.887704432822748	20.651934620634325	34.552388127608154	22.90797281893477
51	19.768158624704633	21.069973141819997	32.4428713302382	26.71899690323717
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	537.5
2	1031.0
3	11359.0
4	21687.0
5	15673.5
6	9660.0
7	9110.0
8	8560.0
9	8144.5
10	7729.0
11	7339.5
12	6950.0
13	6422.5
14	5895.0
15	5519.0
16	5143.0
17	4628.5
18	4114.0
19	3983.0
20	3852.0
21	3573.5
22	3295.0
23	3204.0
24	3113.0
25	3315.0
26	3804.5
27	4092.0
28	4533.0
29	4974.0
30	5380.5
31	5787.0
32	6225.5
33	6664.0
34	7376.5
35	8089.0
36	8472.5
37	8856.0
38	9420.0
39	9984.0
40	10790.5
41	11597.0
42	12498.0
43	13399.0
44	15352.5
45	17306.0
46	45276.0
47	73246.0
48	50398.5
49	27551.0
50	25464.0
51	23377.0
52	21069.0
53	18761.0
54	18338.0
55	17915.0
56	17299.0
57	16683.0
58	15805.0
59	14927.0
60	13341.5
61	11756.0
62	10441.5
63	9127.0
64	7930.0
65	6733.0
66	5814.0
67	4895.0
68	3996.5
69	3098.0
70	2548.0
71	1998.0
72	1709.0
73	1420.0
74	1180.0
75	724.0
76	508.0
77	405.0
78	302.0
79	216.0
80	130.0
81	93.0
82	56.0
83	41.5
84	27.0
85	18.5
86	10.0
87	10.0
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	408814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.34452840079036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43206792319474	34.42945524030423
2	6.535261809622909	5.665356822276016
3	3.3241808687977032	4.322551562308981
4	2.4513595237321804	4.250120899878297
5	1.9020925302532865	4.122265184924738
6	1.4696795809262417	3.82215410013115
7	1.1758688038567526	3.5677235135060688
8	0.8973430224666546	3.1115928098045584
9	0.6797195094966392	2.6515909425553534
>10	2.038565496641173	12.87435946410065
>50	0.05745426686172426	1.7064911675351617
>100	0.028442706367190224	2.3192105074173868
>500	0.002275416509375218	0.7244142537521031
>1k	0.003413124764062827	3.7840658789426564
>5k	0.0	0.0
>10k+	0.002275416509375218	12.648647652562673
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	14272	3.4910741804341336	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	14091	3.4467997671312625	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	12285	3.005034074175542	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10651	2.6053413043584612	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGT	2915	0.71303820319265	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	2728	0.6672961297802913	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTC	2608	0.6379429275905424	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTA	2391	0.5848625536307465	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2390	0.5846179436124986	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	2315	0.5662721922439056	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGT	941	0.23017802717128083	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	771	0.18859432406913657	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTAT	648	0.15850729182464396	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATG	578	0.14138459054729044	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTAGCAGAGT	460	0.11252060839403738	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTT	427	0.10444847779185644	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09123953680646944	0.0
2	0.0	0.0	0.0	0.8101483804370692	0.0
3	0.0	0.0	0.0	0.9182660085026443	0.0
4	0.0	0.0	0.0	1.74749397036305	0.0
5	0.0	0.0	0.0	5.368945290523318	0.0
6	0.0	0.0	0.0	5.659541992201833	0.0
7	0.0	0.0	0.0	6.370378705230252	0.0
8	0.0	0.0	0.0	7.197405176926426	0.0
9	0.0	0.0	0.0	7.3312068569080315	0.0
10	0.0	0.0	0.0	11.377301168746667	0.0
11	0.0	0.0	0.0	11.566873932888795	0.0
12	0.0	0.0	0.0	15.74285616441707	0.0
13	0.0	0.0	0.0	16.0197547050737	0.0
14	0.0	0.0	0.0	16.246263581971263	0.0
15	0.0	0.0	0.0	17.0133605991967	0.0
16	0.0	0.0	0.0	17.365109805437193	0.0
17	0.0	0.0	0.0	17.516768016750895	0.0
18	0.0	0.0	0.0	17.63393621549164	0.0
19	0.0	0.0	0.0	18.605282597954083	0.0
20	0.0	0.0	0.0	18.805862812917365	0.0
21	0.0	0.0	0.0	18.924009451731106	0.0
22	0.0	0.0	0.0	19.185986781274615	0.0
23	0.0	0.0	0.0	19.316853141037246	0.0
24	0.0	0.0	0.0	19.429129139413035	0.0
25	0.0	0.0	0.0	19.526973146712198	0.0
26	0.0	0.0	0.0	19.64218446530696	0.0
27	0.0	0.0	0.0	19.816836018335966	0.0
28	0.0	0.0	0.0	19.918349175908848	0.0
29	0.0	0.0	0.0	20.044812555343018	0.0
30	0.0	0.0	0.0	20.191333956273514	0.0
31	0.0	0.0	0.0	20.318286555744177	0.0
32	0.0	0.0	0.0	20.45233284574403	0.0
33	0.0	0.0	0.0	20.583688425543155	0.0
34	0.0	0.0	0.0	20.80628354214875	0.0
35	0.0	0.0	0.0	20.983136585341988	0.0
36	0.0	0.0	0.0	21.12672266605351	0.0
37	0.0	0.0	0.0	21.288654498133624	0.0
38	0.0	0.0	0.0	21.487033222932677	0.0
39	0.0	0.0	0.0	21.84563150968411	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAGG	65	0.0	45.000004	31
CGCCGGT	65	0.0	45.000004	28
ATAGGCC	20	7.027948E-4	45.0	29
CGTGAAC	20	7.027948E-4	45.0	33
CCAATCC	20	7.027948E-4	45.0	12
TACGAAT	20	7.027948E-4	45.0	12
CTACCGA	20	7.027948E-4	45.0	15
AGCTACG	20	7.027948E-4	45.0	9
CGATCCA	20	7.027948E-4	45.0	34
CACCCGT	80	0.0	45.0	16
CGGGTAT	60	0.0	44.999996	6
CGATTAA	30	2.162058E-6	44.999996	19
CACCGTT	30	2.162058E-6	44.999996	16
CGTTTTT	7750	0.0	44.216125	1
TACGGCT	1675	0.0	43.25373	7
ACGGCTG	1690	0.0	42.736687	8
GATACCT	1620	0.0	42.5	5
CCGATGA	365	0.0	41.91781	18
TGATACC	1650	0.0	41.863636	4
ATACCTG	1665	0.0	41.62162	6
>>END_MODULE