FastQCFastQC Report
Sat 14 Jan 2017
SRR2933462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296072
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG130444.405685103623442No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114043.8517657867005326No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC114023.8510902753384313No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC88822.999945959091032No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT29350.9913129238833798No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC22890.7731227539247211No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT22440.757923748277446No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA21390.7224594017671377No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC21260.7180685779134806No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT17990.607622470209949No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGT8900.3006025561349942No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT5920.19995136318192872No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATG5410.1827258234483504No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT5040.17022886324947986No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAAGCCA3830.12936042584236268No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT3640.12294306790240211No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3410.11517468723823934No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCTGAAGCCAT3210.10841957361722825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3150.10639303953092491No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.10335323840146991No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG207.024793E-445.0000041
AGCCCCT207.024793E-445.00000411
TCGGGCA207.024793E-445.0000045
CGATTCG207.024793E-445.00000410
TCTCGCG207.024793E-445.0000041
GCGATCG207.024793E-445.0000049
GGATTGC207.024793E-445.0000048
ACGCCTG351.2078453E-745.08
ACCGAGG253.8837672E-545.017
TACGCCT351.2078453E-745.07
AGGTTAG253.8837672E-545.040
CTACGGC253.8837672E-545.06
ATGCGGC351.2078453E-745.06
ACCCGTC551.8189894E-1245.017
GGATTAC253.8837672E-545.08
CACCGGT700.045.016
GAATGGT302.1600245E-644.9999961
TTAGCGG302.1600245E-644.9999962
TAGCGGG302.1600245E-644.9999963
CGTTTTT95850.044.4366231
Produced by FastQC (version 0.11.5)