FastQCFastQC Report
Sat 14 Jan 2017
SRR2933461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195806
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170868.725983882005659No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG67253.4345219247622647No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC55562.8375024258705044No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC30201.5423429312687047No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC18290.9340878216193579No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT17580.8978274414471468No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA8090.41316405013125235No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT7800.39835347231443363No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC7370.37639296037915076No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT6450.3294076790292432No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT5980.30540432877439916No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC4980.2543333707853692No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGAAGCCATCGTATGCCG4880.2492262749864662No Hit
TCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC4630.2364585354892087No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC3810.19458034993820414No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGT3720.18998396371919143No Hit
GAATCTTTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC2690.1373808769904906No Hit
GAATGACTCTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT2540.12972023329213608No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT2500.12767739497257488No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC2420.1235917183334525No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2330.1189953321144398No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC2240.11439894589542711No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG2230.11388823631553681No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTGAAGCCATCGTATGC2220.11337752673564652No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA2100.1072490117769629No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATG2090.10673830219707262No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2070.10571688303729201No Hit
GAATCTGTCTCTTATCCACATCTGACGCTGAAGCCATCGTATGCCGTCTTC2030.10367404471773081No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT2020.10316333513784053No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAAGCCA1980.10112049681827932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGC207.018941E-445.03
CCAGGGT207.018941E-445.013
GTGGGCC253.8789192E-545.05
GGGTACC253.8789192E-545.07
GACCGAT207.018941E-445.09
GATCAAT207.018941E-445.09
GTCTAAT253.8789192E-545.021
GGATATC207.018941E-445.08
GGATAAT253.8789192E-545.08
CAGGGTG207.018941E-445.014
CTGCGCG207.018941E-445.01
CGGGGCG207.018941E-445.06
CCTCCTA253.8789192E-545.015
GAGAAAT253.8789192E-545.09
CGAGGGA207.018941E-445.04
GTCAAGT253.8789192E-545.018
CGGGATA207.018941E-445.06
AGCGATA207.018941E-445.031
CGGTGTC502.1827873E-1145.019
CCAAGGT207.018941E-445.013
Produced by FastQC (version 0.11.5)