##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933461.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195806
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.011240717853386	31.0	25.0	31.0	16.0	33.0
2	27.78906672931371	31.0	26.0	33.0	16.0	34.0
3	27.900100099077658	31.0	26.0	33.0	16.0	34.0
4	31.625123847073123	35.0	28.0	37.0	19.0	37.0
5	33.71501894732541	35.0	33.0	37.0	30.0	37.0
6	32.03451886050478	35.0	32.0	37.0	17.0	37.0
7	32.718164918337536	35.0	32.0	36.0	28.0	37.0
8	32.44070661777474	35.0	32.0	37.0	23.0	37.0
9	33.473785277264234	35.0	32.0	38.0	27.0	39.0
10	32.55702583169055	35.0	28.0	39.0	17.0	39.0
11	33.20493243312258	37.0	31.0	39.0	17.0	39.0
12	33.865458668273696	37.0	31.0	39.0	25.0	39.0
13	34.00035238961012	37.0	32.0	39.0	27.0	39.0
14	34.11324474224487	37.0	32.0	40.0	23.0	41.0
15	34.48200259440467	37.0	32.0	40.0	25.0	41.0
16	34.66368752745064	37.0	32.0	40.0	26.0	41.0
17	32.63830526133009	36.0	29.0	40.0	18.0	41.0
18	33.300016342706556	36.0	30.0	39.0	24.0	39.0
19	32.96811640092745	36.0	31.0	37.0	23.0	39.0
20	32.20163835633229	34.0	30.0	35.0	24.0	39.0
21	32.72638734257377	35.0	31.0	37.0	24.0	39.0
22	32.7635159290318	34.0	30.0	37.0	25.0	39.0
23	33.640766881505165	35.0	32.0	38.0	27.0	39.0
24	32.23608061040009	35.0	31.0	37.0	21.0	39.0
25	31.425890932862117	34.0	30.0	37.0	18.0	39.0
26	30.356919604097932	33.0	25.0	36.0	18.0	39.0
27	32.315000561780536	34.0	30.0	37.0	22.0	39.0
28	32.20587724584538	35.0	31.0	37.0	21.0	39.0
29	32.31911688099446	35.0	31.0	37.0	21.0	40.0
30	31.3836195009346	34.0	29.0	37.0	18.0	39.0
31	31.705187787912525	35.0	30.0	37.0	21.0	39.0
32	31.661946007783214	35.0	30.0	37.0	18.0	39.0
33	31.314586887020827	35.0	30.0	37.0	15.0	40.0
34	30.308351123050365	35.0	27.0	37.0	10.0	39.0
35	29.833718067883517	35.0	25.0	37.0	8.0	40.0
36	28.970332880504174	34.0	22.0	37.0	7.0	40.0
37	28.864187001419772	34.0	22.0	37.0	7.0	40.0
38	28.670413572617797	34.0	21.0	37.0	7.0	40.0
39	28.369161312727904	34.0	21.0	37.0	7.0	39.0
40	28.17805889502875	33.0	20.0	37.0	7.0	39.0
41	27.401065340183653	33.0	18.0	37.0	7.0	39.0
42	27.96117075064094	33.0	18.0	37.0	7.0	40.0
43	27.769756800098055	33.0	18.0	37.0	7.0	40.0
44	27.70730723266907	33.0	18.0	37.0	7.0	40.0
45	27.904303238920157	34.0	18.0	38.0	7.0	40.0
46	27.6573496215642	34.0	17.0	38.0	7.0	40.0
47	27.57034513753409	33.0	17.0	37.0	7.0	40.0
48	27.431707914977068	33.0	17.0	37.0	7.0	40.0
49	27.43264762060407	33.0	17.0	37.0	7.0	40.0
50	27.464648682879993	33.0	17.0	37.0	7.0	40.0
51	25.898813110936334	31.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	9.0
11	7.0
12	10.0
13	26.0
14	36.0
15	81.0
16	221.0
17	422.0
18	662.0
19	1139.0
20	1702.0
21	2540.0
22	3359.0
23	4838.0
24	6591.0
25	9304.0
26	11682.0
27	12235.0
28	11737.0
29	11798.0
30	12738.0
31	14247.0
32	16056.0
33	17314.0
34	18859.0
35	19549.0
36	11967.0
37	5485.0
38	1158.0
39	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.366372838421704	2.031091999223721	26.77190688742939	43.83062827492518
2	44.68606682124143	18.22262852006578	25.958346526664144	11.13295813202864
3	9.772427811200883	17.963698763061398	61.69678150822753	10.56709191751019
4	9.860269858942013	2.142937397219697	75.84701183824805	12.149780905590227
5	22.016689989070816	2.5188196480189573	61.79432703798658	13.670163324923648
6	10.34697608857747	15.59911340816931	63.530228900033705	10.523681603219513
7	39.59480301931503	1.0648294740712745	56.26691725483387	3.073450251779823
8	38.99063358630481	13.189074900666986	42.799505633126664	5.020785879901536
9	35.65059293382225	2.3936958009458342	43.1401489229135	18.81556234231842
10	23.584057689754147	15.471946722776625	47.807523773530946	13.136471813938286
11	20.31806992635568	14.277397015413214	54.24757157594762	11.156961482283485
12	15.732919318100569	12.371939572842509	55.117820700080685	16.77732040897623
13	14.464827431232955	14.719160802018324	62.08900646558328	8.72700530116544
14	12.210555345597172	18.723634617938163	52.04845612493999	17.017353911524673
15	7.636129638519759	14.852456002369694	63.82235477973096	13.68905957937959
16	9.235672042736178	14.778403113285599	51.92537511618644	24.060549727791795
17	9.555376239747506	16.529115553149545	59.17489760272924	14.740610604373716
18	9.434338069313505	14.515387679642094	57.88075952728722	18.16951472375719
19	9.838820056586622	17.95042031398425	54.21948254905365	17.991277080375472
20	11.875529861189136	16.171618847226334	61.78360213680888	10.169249154775645
21	13.443918981032246	22.239870075482877	52.652625557950216	11.663585385534663
22	9.994075768873273	14.275354177093655	57.05902781324372	18.67154224078935
23	10.72541188727618	21.953362001164418	52.99633310521639	14.324893006343013
24	12.810128392388384	14.55675515561321	53.12605333850853	19.507063113489885
25	9.087055554988101	23.45893384268102	51.3579767729283	16.09603382940257
26	10.150352900319705	14.672686230248308	58.041633044952654	17.135327824479333
27	12.714625700948897	18.550504070355352	52.19962616058752	16.53524406810823
28	8.229063460772396	18.20628581350929	59.32708905753654	14.237561668181772
29	14.580247796288162	14.446952595936793	54.81037353298673	16.16242607478831
30	14.058813315220167	20.86912556305731	53.546367322758236	11.525693798964282
31	13.593556887940103	18.388098423950236	48.76612565498504	19.252219033124625
32	15.984188431406595	23.416034237970234	47.07210197848891	13.527675352134255
33	14.119587755227112	18.338559594700875	45.41791364922423	22.123939000847777
34	14.485255814428566	19.444756544743267	48.34938663779455	17.720601003033615
35	15.368272678058895	19.372235784398846	43.134020407954814	22.12547112958745
36	14.273822048353981	24.835296160485377	44.08291880739099	16.80796298376965
37	16.009213200821222	25.02272657630512	43.39754655117821	15.570513671695455
38	18.372777136553527	26.517573516644028	38.53661276978234	16.573036577020112
39	14.775849565386148	24.965527103357406	41.025300552587765	19.233322778668683
40	18.543354136236886	19.396239134653687	40.62694708027333	21.43345964883609
41	14.152273168340091	25.817390682614423	38.17911606385913	21.851220085186355
42	13.534825286252719	21.438056035055105	42.94965424961442	22.07746442907776
43	16.766084798218646	23.83277325516072	39.60603863007262	19.795103316548012
44	19.581116002573975	20.000919277243803	40.4548379518503	19.96312676833192
45	14.449506143836247	21.36298172681123	39.92982850372307	24.25768362562945
46	19.779782029151303	24.41600359539544	38.12600226755053	17.67821210790272
47	15.489821558072785	18.51730794766248	48.180341766850866	17.812528727413866
48	17.85083194590564	21.54939072347119	37.626528298417824	22.973249032205345
49	14.539901739476829	17.800782407076394	44.93427167706812	22.72504417637866
50	18.229778454184242	18.15112917888114	40.836338008028356	22.782754358906267
51	16.976497145133447	17.556663227888826	39.58152457023789	25.885315056739834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	153.0
2	275.0
3	11653.0
4	23031.0
5	15176.5
6	7322.0
7	7161.0
8	7000.0
9	6974.5
10	6949.0
11	6510.0
12	6071.0
13	5635.5
14	5200.0
15	4668.5
16	4137.0
17	3796.5
18	3456.0
19	3109.5
20	2763.0
21	2668.0
22	2573.0
23	2485.0
24	2397.0
25	2336.5
26	2297.0
27	2318.0
28	2286.5
29	2255.0
30	2392.5
31	2530.0
32	2545.5
33	2561.0
34	2557.0
35	2553.0
36	2679.5
37	2806.0
38	3281.5
39	3757.0
40	4225.0
41	4693.0
42	5244.5
43	5796.0
44	6788.0
45	7780.0
46	18049.0
47	28318.0
48	22931.0
49	17544.0
50	15559.5
51	13575.0
52	10509.0
53	7443.0
54	6326.5
55	5210.0
56	4395.5
57	3581.0
58	3137.0
59	2693.0
60	2311.0
61	1929.0
62	1753.5
63	1578.0
64	1385.0
65	1192.0
66	983.5
67	775.0
68	670.0
69	565.0
70	473.5
71	382.0
72	287.5
73	193.0
74	170.0
75	107.5
76	68.0
77	62.0
78	56.0
79	36.0
80	16.0
81	12.0
82	8.0
83	5.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	195806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.47048609337814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.99978020045718	39.49981103745544
2	6.299454897133814	5.8547746238623946
3	2.358449094425884	3.2879482753337483
4	1.3781431334622825	2.561719252729743
5	1.0220678740988218	2.3747995464898928
6	0.7220414981536838	2.013217163927561
7	0.6198347107438017	2.016281421406903
8	0.4582820467733427	1.7037271585140394
9	0.35827325479163	1.498421907398139
>10	1.6594865482679795	12.465910135542323
>50	0.061543871988746265	1.967764011317324
>100	0.05055389484789871	4.560636548420375
>500	0.0054949885704237734	1.8227224906284791
>1k	0.0032969931422542637	3.374258194335209
>5k	0.0021979954281695093	6.27202435063277
>10k+	0.0010989977140847546	8.725983882005659
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17086	8.725983882005659	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	6725	3.4345219247622647	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	5556	2.8375024258705044	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	3020	1.5423429312687047	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	1829	0.9340878216193579	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT	1758	0.8978274414471468	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA	809	0.41316405013125235	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	780	0.39835347231443363	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC	737	0.37639296037915076	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	645	0.3294076790292432	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	598	0.30540432877439916	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	498	0.2543333707853692	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGAAGCCATCGTATGCCG	488	0.2492262749864662	No Hit
TCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	463	0.2364585354892087	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	381	0.19458034993820414	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGT	372	0.18998396371919143	No Hit
GAATCTTTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	269	0.1373808769904906	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	254	0.12972023329213608	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT	250	0.12767739497257488	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	242	0.1235917183334525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	233	0.1189953321144398	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	224	0.11439894589542711	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	223	0.11388823631553681	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTGAAGCCATCGTATGC	222	0.11337752673564652	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	210	0.1072490117769629	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATG	209	0.10673830219707262	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	207	0.10571688303729201	No Hit
GAATCTGTCTCTTATCCACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	203	0.10367404471773081	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	202	0.10316333513784053	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAAGCCA	198	0.10112049681827932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4014177297937755	0.0
2	0.0	0.0	0.0	2.035688385442734	0.0
3	0.0	0.0	0.0	2.407484959602872	0.0
4	0.0	0.0	0.0	3.397750835010163	0.0
5	0.0	0.0	0.0	5.7817431539380815	0.0
6	0.0	0.0	0.0	6.261299449455072	0.0
7	0.0	0.0	0.0	6.948714543987416	0.0
8	0.0	0.0	0.0	7.812324443581913	0.0
9	0.0	0.0	0.0	8.124878706474776	0.0
10	0.0	0.0	0.0	12.910738179626774	0.0
11	0.0	0.0	0.0	14.086391632534243	0.0
12	0.0	0.0	0.0	18.24152477452172	0.0
13	0.0	0.0	0.0	18.668477983310012	0.0
14	0.0	0.0	0.0	18.911575743337792	0.0
15	0.0	0.0	0.0	19.61992993064564	0.0
16	0.0	0.0	0.0	20.120425318938132	0.0
17	0.0	0.0	0.0	20.66075605446207	0.0
18	0.0	0.0	0.0	21.13571596376005	0.0
19	0.0	0.0	0.0	22.645884191495664	0.0
20	0.0	0.0	0.0	23.159147319285417	0.0
21	0.0	0.0	0.0	23.668324770436044	0.0
22	0.0	0.0	0.0	24.541127442468564	0.0
23	0.0	0.0	0.0	25.0395799924415	0.0
24	0.0	0.0	0.0	25.377669734328876	0.0
25	0.0	0.0	0.0	25.69533109302064	0.0
26	0.0	0.0	0.0	25.939960981788097	0.0
27	0.0	0.0	0.0	26.21676557408864	0.0
28	0.0	0.0	0.0	26.45884191495664	0.0
29	0.0	0.0	0.0	26.749946375494112	0.0
30	0.0	0.0	0.0	27.05330786594895	0.0
31	0.0	0.0	0.0	27.29027711101805	0.0
32	0.0	0.0	0.0	27.552271125501772	0.0
33	0.0	0.0	0.0	27.800475981328457	0.0
34	0.0	0.0	0.0	28.03744522639756	0.0
35	0.0	0.0	0.0	28.306589174999743	0.0
36	0.0	0.0	0.0	28.543047710488953	0.0
37	0.0	0.0	0.0	28.756524314883098	0.0
38	0.0	0.0	0.0	29.012389814408138	0.0
39	0.0	0.0	0.0	29.294301502507583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGGC	20	7.018941E-4	45.0	3
CCAGGGT	20	7.018941E-4	45.0	13
GTGGGCC	25	3.8789192E-5	45.0	5
GGGTACC	25	3.8789192E-5	45.0	7
GACCGAT	20	7.018941E-4	45.0	9
GATCAAT	20	7.018941E-4	45.0	9
GTCTAAT	25	3.8789192E-5	45.0	21
GGATATC	20	7.018941E-4	45.0	8
GGATAAT	25	3.8789192E-5	45.0	8
CAGGGTG	20	7.018941E-4	45.0	14
CTGCGCG	20	7.018941E-4	45.0	1
CGGGGCG	20	7.018941E-4	45.0	6
CCTCCTA	25	3.8789192E-5	45.0	15
GAGAAAT	25	3.8789192E-5	45.0	9
CGAGGGA	20	7.018941E-4	45.0	4
GTCAAGT	25	3.8789192E-5	45.0	18
CGGGATA	20	7.018941E-4	45.0	6
AGCGATA	20	7.018941E-4	45.0	31
CGGTGTC	50	2.1827873E-11	45.0	19
CCAAGGT	20	7.018941E-4	45.0	13
>>END_MODULE