##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713577
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.979754112029955	31.0	28.0	31.0	25.0	34.0
2	27.73281930331275	31.0	25.0	33.0	16.0	34.0
3	27.701423952846014	31.0	25.0	33.0	16.0	34.0
4	32.74262343096821	35.0	32.0	37.0	28.0	37.0
5	34.08347662550783	35.0	33.0	37.0	30.0	37.0
6	33.14592538716915	35.0	32.0	37.0	28.0	37.0
7	33.6338152715124	35.0	33.0	37.0	29.0	37.0
8	33.141477373850336	35.0	32.0	37.0	27.0	37.0
9	34.59138537256666	37.0	34.0	39.0	28.0	39.0
10	34.60732618904477	37.0	32.0	39.0	28.0	39.0
11	34.995441276834875	37.0	34.0	39.0	28.0	39.0
12	35.12688609638483	37.0	34.0	39.0	30.0	39.0
13	35.17152598808538	37.0	34.0	39.0	29.0	39.0
14	35.96421689600422	38.0	34.0	40.0	29.0	41.0
15	36.1413260236807	38.0	34.0	40.0	27.0	41.0
16	36.236846198798446	38.0	34.0	40.0	30.0	41.0
17	35.35440464028409	37.0	33.0	40.0	27.0	41.0
18	35.18410346746042	37.0	33.0	39.0	27.0	40.0
19	34.57144498771681	37.0	33.0	38.0	27.0	40.0
20	33.34550581086554	35.0	32.0	37.0	25.0	39.0
21	33.96988692180381	35.0	33.0	38.0	27.0	40.0
22	34.50437864449106	35.0	33.0	38.0	28.0	40.0
23	34.65795422217925	35.0	33.0	39.0	28.0	40.0
24	34.24802509049479	35.0	33.0	38.0	27.0	40.0
25	33.26423497394114	35.0	32.0	38.0	24.0	40.0
26	33.281197404064315	35.0	32.0	38.0	24.0	40.0
27	34.03006543092056	35.0	33.0	38.0	25.0	40.0
28	33.53000026626419	35.0	33.0	38.0	24.0	40.0
29	33.51746482860294	35.0	33.0	39.0	23.0	40.0
30	32.866767006223576	35.0	32.0	38.0	21.0	40.0
31	32.75097431671705	35.0	31.0	38.0	21.0	40.0
32	32.19444572905237	35.0	31.0	38.0	16.0	40.0
33	31.28444442575924	35.0	30.0	39.0	12.0	40.0
34	30.762790841072512	35.0	25.0	39.0	8.0	40.0
35	30.22166213316853	35.0	23.0	39.0	7.0	40.0
36	29.579533813449704	35.0	21.0	39.0	7.0	40.0
37	29.51347086579304	35.0	21.0	39.0	7.0	40.0
38	29.163622145893154	35.0	20.0	39.0	7.0	40.0
39	29.02385306701309	35.0	18.0	39.0	7.0	40.0
40	28.87404722966127	35.0	19.0	39.0	7.0	40.0
41	28.27234762331185	34.0	18.0	38.0	7.0	40.0
42	28.59751505443701	35.0	18.0	39.0	7.0	40.0
43	28.60826371926225	35.0	18.0	39.0	7.0	40.0
44	28.71759039318812	35.0	18.0	39.0	7.0	40.0
45	28.698339492444404	35.0	18.0	39.0	7.0	40.0
46	28.49168625109834	35.0	18.0	39.0	7.0	40.0
47	28.31533247287959	35.0	18.0	39.0	7.0	40.0
48	28.177018037296605	34.0	18.0	38.0	7.0	40.0
49	28.365711058512257	35.0	18.0	39.0	7.0	40.0
50	28.27753136662196	34.0	18.0	39.0	7.0	40.0
51	27.08869960775081	33.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	30.0
10	32.0
11	53.0
12	67.0
13	88.0
14	146.0
15	203.0
16	433.0
17	872.0
18	1809.0
19	3329.0
20	5304.0
21	7952.0
22	11330.0
23	15971.0
24	22987.0
25	32422.0
26	40303.0
27	39623.0
28	34797.0
29	31774.0
30	32454.0
31	36672.0
32	42516.0
33	49599.0
34	56746.0
35	64857.0
36	67675.0
37	63453.0
38	43924.0
39	6143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.115276977817395	2.2284911088782287	27.2304180207602	37.42581389254418
2	38.72784576857158	23.505101761968223	26.49566900278456	11.271383466675635
3	9.580465738105348	23.670185558110756	54.87368567092269	11.875663032861205
4	8.986976878458806	2.4024036649163296	75.23364682437915	13.376972632245716
5	24.615563562166383	2.771529911978665	56.87375013488383	15.739156390971122
6	11.357989397079784	19.466434596406554	60.68665329740168	8.48892270911198
7	44.42337687453492	1.0199319765070902	51.887462740531156	2.669228408426841
8	41.88461791789814	15.1673890834486	38.700238376517184	4.247754622136084
9	39.57050185193749	2.360922507311755	36.85446700215954	21.214108638591213
10	27.01516444616348	14.16357309722707	44.68655800285043	14.13470445375902
11	23.196095165623333	14.424652139853162	48.67996025656657	13.699292437956942
12	16.165879785923593	12.634095549604318	51.78151762178433	19.418507042687754
13	15.626764876110077	14.762247101574182	59.77687061102025	9.834117411295487
14	14.068979241203122	22.369414933497016	47.74915671329093	15.812449112008936
15	9.806089602103206	14.9357392404744	61.358900300878524	13.899270856543863
16	10.857693002997575	17.794295499995094	47.779146469126665	23.568865027880666
17	12.16084599139266	18.473829733861937	54.33457076110917	15.030753513636231
18	12.177662676908028	17.261907264387723	52.22954215172294	18.330887906981307
19	12.107312875835403	20.355476703985694	48.06194706387678	19.475263356302122
20	14.866510551769466	18.174632870734342	55.86713136774307	11.091725209753116
21	14.219768854657591	25.038082785740013	48.963741824638404	11.77840653496399
22	12.105491068237907	14.30048894513136	52.99442106458028	20.599598922050458
23	13.577932024154366	23.23883757464156	50.85463797179561	12.32859242940846
24	15.93198771821401	17.364909463169358	47.28375494165311	19.41934787696352
25	10.627164272391067	28.757373065555647	46.027128116517204	14.58833454553608
26	12.387871245850132	15.49825737096347	52.463714497524435	19.650156885661954
27	17.644626998908315	19.05330468891234	45.769832828132074	17.53223548404727
28	10.164285003580552	18.86663947969175	53.49121398251345	17.47786153421425
29	17.0495966097562	14.208417591934719	47.840807649349685	20.9011781489594
30	16.074228849864834	21.581693356147973	48.9078263452998	13.436251448687386
31	15.391051000802996	14.990113190307422	48.88764632268136	20.731189486208216
32	17.57609900543319	20.27447633542	47.404554799271835	14.744869859874967
33	14.212061207129715	17.112238763300947	40.9182190569483	27.757480972621035
34	16.42317507430873	18.54193731019918	41.27122931372508	23.763658301767013
35	18.128947541750925	20.333755151861677	42.33474453352616	19.202552772861235
36	14.278066697777536	23.968541586962587	39.660611258490675	22.092780456769205
37	17.612535157383157	20.79537316925854	45.13430225469711	16.457789418661196
38	15.839355808833524	22.08997767584998	38.2550166274978	23.815649887818694
39	17.99455419667394	21.71930989928207	39.35426730401905	20.931868600024945
40	15.747284455636883	21.036412328312153	42.77436072070708	20.44194249534388
41	15.617375560030663	28.955950093682954	34.31710943598238	21.109564910304005
42	15.377457513344742	21.471544066022307	46.30950829412944	16.841490126503516
43	17.99161127670875	24.74715412632414	34.11320712410854	23.14802747285857
44	19.669776352096548	21.65064176676098	36.08510363983144	22.594478241311027
45	15.393293225538379	20.564424021514146	38.72097895531947	25.32130379762801
46	22.163550674979714	25.286269036137654	34.69170110583721	17.858479183045418
47	13.863815677915628	20.511451462140737	45.299946607023486	20.324786252920145
48	18.65713160597945	23.65925471252577	33.0502524604913	24.63336122100348
49	15.291692417216362	20.26774966121386	42.98765234866034	21.452905572909444
50	17.292877993545194	21.181035823744317	37.68843446467585	23.837651718034632
51	17.607630290774505	20.705123623659393	34.30898137131662	27.37826471424948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	962.0
2	1885.0
3	23194.5
4	44504.0
5	33809.5
6	23115.0
7	22188.5
8	21262.0
9	21111.0
10	20960.0
11	20259.5
12	19559.0
13	18641.0
14	17723.0
15	16699.5
16	15676.0
17	14852.0
18	14028.0
19	13201.0
20	12374.0
21	11701.0
22	11028.0
23	10460.0
24	9892.0
25	9550.5
26	9383.0
27	9557.0
28	9620.0
29	9683.0
30	10373.0
31	11063.0
32	11707.0
33	12351.0
34	13284.5
35	14218.0
36	15143.5
37	16069.0
38	17309.5
39	18550.0
40	19543.0
41	20536.0
42	21569.0
43	22602.0
44	25741.0
45	28880.0
46	86014.5
47	143149.0
48	95012.0
49	46875.0
50	42226.0
51	37577.0
52	30814.0
53	24051.0
54	21905.0
55	19759.0
56	17647.5
57	15536.0
58	14018.5
59	12501.0
60	11254.0
61	10007.0
62	8361.0
63	6715.0
64	5609.5
65	4504.0
66	3666.5
67	2829.0
68	2339.5
69	1850.0
70	1586.0
71	1322.0
72	1149.5
73	977.0
74	764.0
75	442.5
76	334.0
77	233.5
78	133.0
79	104.0
80	75.0
81	59.0
82	43.0
83	29.0
84	15.0
85	8.5
86	2.0
87	1.5
88	1.0
89	3.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.407681063877696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91934616454186	39.87317614020962
2	6.087611948192514	5.650239074647387
3	2.242417578539206	3.121961993906412
4	1.2688378451894986	2.355352881653283
5	0.789823423838073	1.8326936775128595
6	0.569555313573843	1.5859044804343054
7	0.4385860655346973	1.4247633573876637
8	0.3598070201015671	1.3358247546734208
9	0.3063087816406356	1.2793572220899214
>10	1.9058144760611577	14.542671543062907
>50	0.06731926733841105	2.1777618556435665
>100	0.037502055756743105	3.4485269955914277
>500	0.0027665450968088934	0.8051413238652242
>1k	0.0018443633978725959	2.3062933704693624
>5k	0.0012295755985817305	3.3435333715012625
>10k+	0.0012295755985817305	14.916797957351354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	29953	4.197584843681901	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	27758	3.889979637796622	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23711	3.3228369187908244	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	23144	3.243378079730709	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	6878	0.9638763581225291	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC	6060	0.8492426185261016	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT	5480	0.7679619718684879	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	5020	0.7034980107262426	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA	4990	0.6992938393474005	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	4922	0.6897643842220251	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGT	1945	0.27257044439492867	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT	1748	0.24496305234053228	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTAT	1324	0.18554409685289747	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATG	1238	0.17349213890021678	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	773	0.10832748252816445	TruSeq Adapter, Index 16 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	767	0.10748664825239602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.116175269102003	0.0
2	0.0	0.0	0.0	0.9209938100583399	0.0
3	0.0	0.0	0.0	1.0698214768693497	0.0
4	0.0	0.0	0.0	2.246849323899173	0.0
5	0.0	0.0	0.0	6.752039373466354	0.0
6	0.0	0.0	0.0	7.139383696503671	0.0
7	0.0	0.0	0.0	8.043420682000681	0.0
8	0.0	0.0	0.0	9.131320095799051	0.0
9	0.0	0.0	0.0	9.280428040701985	0.0
10	0.0	0.0	0.0	14.232801785932002	0.0
11	0.0	0.0	0.0	14.501868754177895	0.0
12	0.0	0.0	0.0	18.48027612997616	0.0
13	0.0	0.0	0.0	18.81268594699661	0.0
14	0.0	0.0	0.0	19.08259374951827	0.0
15	0.0	0.0	0.0	19.995039077772965	0.0
16	0.0	0.0	0.0	20.44180235629792	0.0
17	0.0	0.0	0.0	20.668827610755393	0.0
18	0.0	0.0	0.0	20.862079355136167	0.0
19	0.0	0.0	0.0	21.96917781823125	0.0
20	0.0	0.0	0.0	22.227313940892152	0.0
21	0.0	0.0	0.0	22.439484456477718	0.0
22	0.0	0.0	0.0	22.829911838526186	0.0
23	0.0	0.0	0.0	23.056516675845774	0.0
24	0.0	0.0	0.0	23.245424109801746	0.0
25	0.0	0.0	0.0	23.398315808945636	0.0
26	0.0	0.0	0.0	23.592128109510256	0.0
27	0.0	0.0	0.0	23.855869794009617	0.0
28	0.0	0.0	0.0	24.034687216656366	0.0
29	0.0	0.0	0.0	24.241392309449434	0.0
30	0.0	0.0	0.0	24.43268210718675	0.0
31	0.0	0.0	0.0	24.636164001922708	0.0
32	0.0	0.0	0.0	24.860386475460952	0.0
33	0.0	0.0	0.0	25.077321718609205	0.0
34	0.0	0.0	0.0	25.328310749926075	0.0
35	0.0	0.0	0.0	25.540901682649526	0.0
36	0.0	0.0	0.0	25.76386290477412	0.0
37	0.0	0.0	0.0	26.01877582937791	0.0
38	0.0	0.0	0.0	26.280695706279772	0.0
39	0.0	0.0	0.0	26.551864760215086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCG	50	2.1827873E-11	45.000004	27
CAGTAGC	30	2.164339E-6	45.000004	27
ACCGGTC	80	0.0	45.0	17
ACTAGAC	20	7.031486E-4	45.0	30
TACGATA	20	7.031486E-4	45.0	12
CGTTTTT	20225	0.0	44.39926	1
CGATGAA	865	0.0	43.699425	19
ACCGGTG	165	0.0	43.636364	17
GATACCT	3375	0.0	43.4	5
CACCGGT	260	0.0	43.26923	16
CCGATGA	885	0.0	43.220337	18
TGATACC	3405	0.0	42.687225	4
ACGGCTG	2740	0.0	42.61861	8
CGGCTGT	2730	0.0	42.28022	9
TACGGCT	2770	0.0	42.238266	7
AATGATC	670	0.0	41.97761	2
GAATCTG	3265	0.0	41.554363	1
CGGTGAA	130	0.0	41.53846	19
ATACCTG	3545	0.0	41.445698	6
ACCCGTC	125	0.0	41.4	17
>>END_MODULE