FastQCFastQC Report
Sat 14 Jan 2017
SRR2933459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437538
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227915.208918996749996No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG146443.3469092970210585No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC97392.2258638106861577No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC90762.0743341149797274No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT31830.727479670337205No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC30580.6989107231829007TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT29470.6735414981098784No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC22460.5133268424685399No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA19090.43630496094053545No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT14330.32751441017694466No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGTTCCTATCGTATGCCG12370.2827183010389955No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT8270.18901215437287733TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGT8030.1835269165192509No Hit
GAATCTTTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC7670.17529905973881127No Hit
GAATCTGTCTCTTATCCACATCTGACGCCGTTCCTATCGTATGCCGTCTTC6200.14170197788534938No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT6020.13758804949512957No Hit
GAATGACTCTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT5950.13598818845448854No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCCGTTCCTATCGTATGC5200.11884682016190594No Hit
GAATCTGTCTCTTATCCCCATCTGACGCCGTTCCTATCGTATGCCGTCTTC4960.1133615823082795No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC4780.10924765391805968TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4690.10719068972294978No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCC4630.10581938025954318No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG4600.10513372552783988No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC4420.10101979713762003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC253.8868307E-545.0000049
CACGACC502.1827873E-1145.00000427
GGCGCGA253.8868307E-545.0000048
TATACGG502.1827873E-1145.00000427
CAGACGT253.8868307E-545.00000423
ACCGAGG207.028491E-445.017
GCGATAT207.028491E-445.09
GATTGTA207.028491E-445.09
GGCGATA453.8380676E-1045.08
TCGCGCG207.028491E-445.01
GCGACCG600.044.99999611
AGACGTG302.1624073E-644.99999624
ACGATAT302.1624073E-644.99999613
TACGATA302.1624073E-644.99999612
ACCCGTC302.1624073E-644.99999617
CGTTTTT129750.044.531791
ACCGGTG1350.043.33333617
TGATACC19050.043.2283444
GATACCT19000.043.2236825
ACCCCGT1200.043.12499639
Produced by FastQC (version 0.11.5)