##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437538
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.74655229945742	30.0	25.0	31.0	16.0	33.0
2	27.587512398923064	31.0	26.0	33.0	16.0	34.0
3	27.74530440784572	31.0	25.0	33.0	16.0	34.0
4	31.400246378600258	35.0	28.0	35.0	19.0	37.0
5	33.51937203168639	35.0	33.0	37.0	28.0	37.0
6	31.812784261024184	35.0	32.0	37.0	17.0	37.0
7	32.316710777121074	35.0	32.0	35.0	26.0	37.0
8	32.322260009416325	35.0	32.0	35.0	21.0	37.0
9	32.92893874360627	35.0	31.0	37.0	25.0	39.0
10	31.91559133149578	35.0	28.0	39.0	17.0	39.0
11	32.66459370386115	35.0	30.0	39.0	17.0	39.0
12	33.41235961219368	35.0	31.0	39.0	25.0	39.0
13	33.617452655540774	35.0	32.0	39.0	26.0	39.0
14	33.66636726410049	36.0	32.0	40.0	21.0	41.0
15	34.092135997330516	36.0	32.0	40.0	25.0	41.0
16	34.22561697498274	36.0	32.0	40.0	25.0	41.0
17	32.18995150135531	35.0	27.0	39.0	18.0	41.0
18	32.892957868802256	36.0	30.0	39.0	23.0	39.0
19	32.72988403292971	36.0	30.0	37.0	21.0	39.0
20	31.987751920976006	34.0	30.0	36.0	23.0	39.0
21	32.52701936746065	35.0	31.0	37.0	23.0	39.0
22	32.90141884819147	34.0	31.0	37.0	25.0	39.0
23	33.692881532575456	35.0	32.0	38.0	26.0	40.0
24	32.232151264575876	35.0	31.0	37.0	21.0	39.0
25	31.393513249134934	34.0	29.0	37.0	18.0	39.0
26	30.74328858293451	33.0	25.0	37.0	19.0	39.0
27	32.43529247745339	34.0	30.0	37.0	22.0	39.0
28	32.21176217837079	35.0	30.0	37.0	21.0	39.0
29	32.35390983183175	35.0	30.0	38.0	21.0	40.0
30	31.488197596551615	34.0	30.0	37.0	18.0	40.0
31	31.664890820911555	34.0	30.0	37.0	20.0	39.0
32	31.636744694175135	35.0	30.0	37.0	18.0	39.0
33	31.262566908474234	35.0	30.0	38.0	15.0	40.0
34	30.327098446306376	35.0	26.0	38.0	10.0	40.0
35	29.696501789558848	35.0	24.0	38.0	8.0	40.0
36	28.952342425115077	34.0	22.0	38.0	7.0	40.0
37	29.129245916926074	35.0	22.0	38.0	7.0	40.0
38	28.880092700519725	34.0	22.0	38.0	7.0	40.0
39	28.68418971609323	34.0	21.0	38.0	7.0	40.0
40	28.427149184756523	33.0	21.0	37.0	7.0	40.0
41	27.64143685805576	33.0	18.0	37.0	7.0	39.0
42	28.37812030040819	33.0	21.0	38.0	7.0	40.0
43	28.478703564033296	34.0	21.0	38.0	7.0	40.0
44	28.4946861758293	34.0	20.0	38.0	7.0	40.0
45	28.64237391952242	34.0	20.0	38.0	7.0	40.0
46	28.379427615429975	34.0	20.0	38.0	7.0	40.0
47	28.38627959171546	34.0	20.0	38.0	7.0	40.0
48	28.230366276757675	34.0	20.0	38.0	7.0	40.0
49	28.06908199973488	34.0	19.0	37.0	7.0	40.0
50	28.182212744950153	34.0	20.0	38.0	7.0	40.0
51	26.381985107579226	31.0	17.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	14.0
11	22.0
12	45.0
13	57.0
14	91.0
15	197.0
16	437.0
17	928.0
18	1658.0
19	2766.0
20	4063.0
21	5998.0
22	8385.0
23	11251.0
24	15166.0
25	20740.0
26	24951.0
27	25418.0
28	24092.0
29	24918.0
30	28008.0
31	32001.0
32	36568.0
33	40231.0
34	43130.0
35	41237.0
36	28443.0
37	13954.0
38	2710.0
39	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.37322015459229	2.249633174718539	31.556801923490074	36.8203447471991
2	39.91744717030292	18.71106052502868	29.119985007016535	12.25150729765186
3	10.488231879288199	18.621011203598318	58.18603184180574	12.704725075307744
4	10.083924139160484	2.4882410213512887	72.67551618373719	14.752318655751044
5	22.13636301304115	2.876778702649827	59.22434165718178	15.762516627127244
6	12.310016501423876	15.519109197372572	61.6504166495253	10.520457651678255
7	46.32146236441177	1.0492802910832888	49.54563946445795	3.0836178800469902
8	45.87075865410547	12.181570515018123	36.79657538316672	5.151095447709684
9	42.45368402287344	2.3741937843113057	36.42906444697375	18.743057745841504
10	25.974886752693482	15.118686834057844	43.71346031658965	15.192966096659033
11	21.605437699125563	15.650297802705138	49.07962279847693	13.664641699692371
12	16.63786916793513	13.51928289657127	51.43576100818672	18.407086927306885
13	15.45511475574693	15.927530865890505	58.209115551106414	10.408238827256147
14	14.088147772307776	20.03277429617542	48.780448783877056	17.09862914763975
15	10.137176656656107	16.02466528621514	59.25291060433609	14.585247452792672
16	11.127262089235677	16.41183165805027	48.336373069310554	24.1245331834035
17	12.54885289963386	17.833422468448454	53.48015486654874	16.13756976536895
18	12.323272492903474	16.095287723580583	52.70605067445571	18.875389109060244
19	12.812601419762398	20.086941019979978	47.976404335166315	19.124053225091306
20	14.67735373841815	17.887589192253014	55.45735456120383	11.977702508125008
21	14.91299041454685	23.35248595550558	48.284263309701096	13.450260320246471
22	12.244879301912063	15.502653483811693	51.66568389488456	20.58678331939169
23	13.424662543596213	22.74956689476114	49.350684969076966	14.475085592565673
24	15.123257865602529	16.942756971965863	47.738253591688036	20.19573157074357
25	11.141203735446977	24.883095868244588	46.36191599358227	17.61378440272616
26	12.447604550919007	16.512165800456188	51.809899940119486	19.230329708505316
27	15.66903903203836	18.716774314459546	46.94106569029433	18.67312096320777
28	10.466290927873693	18.184934794235012	53.63122745910069	17.7175468187906
29	16.57821720627694	14.253847665802741	48.59372214527652	20.574212982643793
30	15.120286695098484	20.568270641635696	49.14201737906193	15.169425284203886
31	14.643528104987451	15.529851121502588	48.82273082566543	21.00388994784453
32	16.37252078676595	19.8766735689243	47.644090341867454	16.1067153024423
33	13.334156119011379	17.04994766168881	43.06025990885363	26.55563631044618
34	15.368265156397845	18.32366560161632	42.58830090186452	23.719768340121313
35	16.891332867088117	19.725372424795104	42.45688374495472	20.926410963162056
36	14.47599979887461	21.31654850550124	41.48942491852136	22.71802677710279
37	16.656838948845586	20.110938935589594	45.85201742477224	17.38020469079257
38	15.404833408755353	21.125936490087717	40.38574935205628	23.083480749100648
39	16.32155378504267	20.680260914480574	40.841709748638976	22.156475551837783
40	15.623557268168708	19.644922269608582	43.18299210582852	21.548528356394186
41	15.135828202350424	26.20640950043196	36.75429334137835	21.903468955839266
42	15.37466460056041	20.434110865799084	45.92675379052791	18.2644707431126
43	17.101600318143795	22.75939461258222	36.50197240011153	23.63703266916245
44	19.38048809474834	20.05768641809397	37.762662900136675	22.799162587021012
45	15.55202062449433	18.913328670881157	40.31375560522743	25.22089509939708
46	20.173333516174598	23.47521815248047	37.02558406355562	19.32586426778931
47	13.742349235952078	18.93664093175907	45.67169023033428	21.649319601954573
48	17.889646156448123	21.40545506904543	36.12943332921941	24.575465445287037
49	15.043036261993246	18.436341529192894	43.63735264137058	22.883269567443286
50	16.73271807248742	19.147594037546455	38.92279070617866	25.196897183787463
51	17.171994203932	18.7071751482157	36.59659275308659	27.52423789476571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	254.5
2	470.0
3	16968.0
4	33466.0
5	23679.0
6	13892.0
7	13194.0
8	12496.0
9	12283.5
10	12071.0
11	11626.0
12	11181.0
13	10453.5
14	9726.0
15	9180.5
16	8635.0
17	8122.0
18	7609.0
19	7065.0
20	6521.0
21	6131.0
22	5741.0
23	5484.5
24	5228.0
25	5048.5
26	4901.0
27	4933.0
28	5004.5
29	5076.0
30	5395.0
31	5714.0
32	6251.0
33	6788.0
34	7404.0
35	8020.0
36	8634.0
37	9248.0
38	10129.5
39	11011.0
40	11863.5
41	12716.0
42	14083.5
43	15451.0
44	17649.5
45	19848.0
46	44207.0
47	68566.0
48	54272.5
49	39979.0
50	35705.5
51	31432.0
52	25189.5
53	18947.0
54	16234.5
55	13522.0
56	11569.5
57	9617.0
58	8547.5
59	7478.0
60	6588.5
61	5699.0
62	4776.5
63	3854.0
64	3244.5
65	2635.0
66	2152.5
67	1670.0
68	1418.0
69	1166.0
70	997.0
71	828.0
72	715.5
73	603.0
74	499.5
75	305.0
76	214.0
77	150.5
78	87.0
79	66.0
80	45.0
81	40.0
82	35.0
83	22.5
84	10.0
85	6.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	437538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.408015808075454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77175860873876	43.23584163835736
2	6.470588839868316	6.523390890552775
3	2.3864688595291876	3.608914799898814
4	1.263531062592535	2.5476837510863555
5	0.8391464614092693	2.1149854046004517
6	0.6138814663721552	1.8566727996703285
7	0.4573759440320602	1.613878967190107
8	0.35703250640610007	1.4397840181532395
9	0.3136710480893797	1.4230381635562543
>10	1.4221174273533566	11.253911452788909
>50	0.050589455739411295	1.7633220051272356
>100	0.045484097820745986	4.604710588418333
>500	0.0032488641300532843	1.1075449611610806
>1k	0.0032488641300532843	3.7463281502054047
>5k	9.282468943009385E-4	4.401871238750683
>10k+	9.282468943009385E-4	8.75812117048269
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22791	5.208918996749996	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	14644	3.3469092970210585	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	9739	2.2258638106861577	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	9076	2.0743341149797274	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	3183	0.727479670337205	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	3058	0.6989107231829007	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT	2947	0.6735414981098784	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC	2246	0.5133268424685399	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA	1909	0.43630496094053545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	1433	0.32751441017694466	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGTTCCTATCGTATGCCG	1237	0.2827183010389955	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	827	0.18901215437287733	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGT	803	0.1835269165192509	No Hit
GAATCTTTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	767	0.17529905973881127	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	620	0.14170197788534938	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT	602	0.13758804949512957	No Hit
GAATGACTCTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	595	0.13598818845448854	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCCGTTCCTATCGTATGC	520	0.11884682016190594	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCCGTTCCTATCGTATGCCGTCTTC	496	0.1133615823082795	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	478	0.10924765391805968	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	469	0.10719068972294978	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCC	463	0.10581938025954318	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	460	0.10513372552783988	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	442	0.10101979713762003	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22352344253527692	0.0
2	0.0	0.0	0.0	1.269375459960049	0.0
3	0.0	0.0	0.0	1.5804341565761146	0.0
4	0.0	0.0	0.0	2.8744931868774826	0.0
5	0.0	0.0	0.0	5.692077031023591	0.0
6	0.0	0.0	0.0	6.236029784841545	0.0
7	0.0	0.0	0.0	6.947282293195105	0.0
8	0.0	0.0	0.0	7.970279152896434	0.0
9	0.0	0.0	0.0	8.252311799203726	0.0
10	0.0	0.0	0.0	12.747007117096116	0.0
11	0.0	0.0	0.0	13.714237391952242	0.0
12	0.0	0.0	0.0	17.05497579638797	0.0
13	0.0	0.0	0.0	17.48373855527977	0.0
14	0.0	0.0	0.0	17.72074654087188	0.0
15	0.0	0.0	0.0	18.509249482330677	0.0
16	0.0	0.0	0.0	19.026004598457735	0.0
17	0.0	0.0	0.0	19.53338909991818	0.0
18	0.0	0.0	0.0	20.08236998843529	0.0
19	0.0	0.0	0.0	21.3302616001353	0.0
20	0.0	0.0	0.0	21.797421024002485	0.0
21	0.0	0.0	0.0	22.348915979869176	0.0
22	0.0	0.0	0.0	23.060168488222736	0.0
23	0.0	0.0	0.0	23.650745763796515	0.0
24	0.0	0.0	0.0	24.07927997111108	0.0
25	0.0	0.0	0.0	24.45753283143407	0.0
26	0.0	0.0	0.0	24.82390100974087	0.0
27	0.0	0.0	0.0	25.20238242164109	0.0
28	0.0	0.0	0.0	25.581778039850253	0.0
29	0.0	0.0	0.0	25.97694371688859	0.0
30	0.0	0.0	0.0	26.378737389666725	0.0
31	0.0	0.0	0.0	26.790587331843177	0.0
32	0.0	0.0	0.0	27.187809973076625	0.0
33	0.0	0.0	0.0	27.570633864944302	0.0
34	0.0	0.0	0.0	27.93860190429174	0.0
35	0.0	0.0	0.0	28.320740141427716	0.0
36	0.0	0.0	0.0	28.693736315474315	0.0
37	0.0	0.0	0.0	29.07541744945582	0.0
38	0.0	0.0	0.0	29.477211122233953	0.0
39	0.0	0.0	0.0	29.889061064410406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	25	3.8868307E-5	45.000004	9
CACGACC	50	2.1827873E-11	45.000004	27
GGCGCGA	25	3.8868307E-5	45.000004	8
TATACGG	50	2.1827873E-11	45.000004	27
CAGACGT	25	3.8868307E-5	45.000004	23
ACCGAGG	20	7.028491E-4	45.0	17
GCGATAT	20	7.028491E-4	45.0	9
GATTGTA	20	7.028491E-4	45.0	9
GGCGATA	45	3.8380676E-10	45.0	8
TCGCGCG	20	7.028491E-4	45.0	1
GCGACCG	60	0.0	44.999996	11
AGACGTG	30	2.1624073E-6	44.999996	24
ACGATAT	30	2.1624073E-6	44.999996	13
TACGATA	30	2.1624073E-6	44.999996	12
ACCCGTC	30	2.1624073E-6	44.999996	17
CGTTTTT	12975	0.0	44.53179	1
ACCGGTG	135	0.0	43.333336	17
TGATACC	1905	0.0	43.228344	4
GATACCT	1900	0.0	43.223682	5
ACCCCGT	120	0.0	43.124996	39
>>END_MODULE