FastQCFastQC Report
Sat 14 Jan 2017
SRR2933455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735390
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400405.444730007207061No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG137671.872067882348142No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC107491.4616733977889282No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC80871.099688600606481No Hit
GAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT35340.4805613348019418RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC28030.3811582969580767RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTT25020.3402276343164851No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC22970.31235126939447094No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGC15170.20628510042290488No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTA13810.1877915119868369No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT13310.1809923985912237No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT11810.16059505840438407No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCCACCATTTCGTATGCCG11680.15882728892152462No Hit
GAATCTTTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC9130.12415181060389725No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC8620.11721671494037177No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACCATTTCGT8130.11055358381267084No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGCCG7570.10293857680958403No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7480.10171473639837365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA207.0316275E-445.018
TCTAGCG207.0316275E-445.01
CGTTTTT262150.044.467861
ACGGCTG11950.042.175738
TGATACC18450.041.951224
GATACCT18400.041.9429365
CTTCGCG650.041.538461
ACCGGTC650.041.5384617
TACGGCT12200.041.49597
CGACCAC10000.041.412
GCGATTT2500.041.49
GAATGAT50850.041.2831841
GCGACCA10250.041.2682911
TTGCGCG603.6379788E-1241.2499961
ACGGGAG556.002665E-1140.9090925
CGATGAA1650.040.90909219
ATGATAC48950.040.8171583
CGGGCGA2500.040.5000046
AATGATA49250.040.4771582
ACGTGAG2450.040.40816544
Produced by FastQC (version 0.11.5)