##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	735390
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.48913773643917	31.0	28.0	31.0	16.0	33.0
2	28.693082582031302	31.0	30.0	33.0	16.0	34.0
3	28.82519071513075	31.0	30.0	33.0	16.0	34.0
4	32.09366458613797	35.0	30.0	37.0	19.0	37.0
5	33.68888480942085	35.0	33.0	37.0	28.0	37.0
6	32.74539632031983	35.0	33.0	37.0	17.0	37.0
7	32.419982594269705	35.0	32.0	36.0	26.0	37.0
8	33.02882416132936	35.0	32.0	37.0	27.0	37.0
9	33.26113898747603	35.0	32.0	38.0	25.0	39.0
10	32.5483947293273	35.0	30.0	39.0	17.0	39.0
11	33.24690028420294	37.0	31.0	39.0	17.0	39.0
12	33.88721766681625	37.0	31.0	39.0	25.0	39.0
13	34.094541671766	37.0	32.0	39.0	27.0	39.0
14	34.20110009654741	37.0	32.0	40.0	23.0	41.0
15	34.74200356273542	37.0	32.0	40.0	26.0	41.0
16	34.88430220699221	37.0	32.0	40.0	26.0	41.0
17	32.945964726199705	36.0	30.0	40.0	18.0	41.0
18	33.45027400426984	36.0	31.0	39.0	24.0	39.0
19	33.19022423475979	36.0	32.0	37.0	23.0	39.0
20	32.53328166007152	35.0	31.0	36.0	25.0	39.0
21	32.84903384598648	35.0	32.0	37.0	25.0	39.0
22	32.79793442935041	34.0	30.0	37.0	25.0	39.0
23	33.82826935367628	35.0	32.0	38.0	27.0	40.0
24	32.10108377867526	35.0	31.0	37.0	21.0	39.0
25	31.302871945498307	34.0	29.0	37.0	18.0	39.0
26	30.19830838058717	33.0	25.0	36.0	18.0	39.0
27	32.20284474904472	34.0	30.0	37.0	22.0	39.0
28	32.14878635825888	35.0	31.0	37.0	21.0	39.0
29	32.10373951236759	35.0	30.0	38.0	21.0	40.0
30	31.24697643427297	34.0	28.0	37.0	18.0	40.0
31	31.45787677286882	34.0	30.0	37.0	18.0	39.0
32	31.177668991963447	35.0	30.0	37.0	16.0	39.0
33	30.547747453732033	35.0	27.0	38.0	12.0	40.0
34	29.21387155114973	34.0	22.0	38.0	8.0	40.0
35	28.544047376222142	34.0	21.0	38.0	7.0	40.0
36	27.769168740396253	33.0	18.0	38.0	7.0	40.0
37	27.89897333387726	34.0	17.0	38.0	7.0	40.0
38	27.78190076014088	34.0	16.0	38.0	7.0	40.0
39	27.338676076639608	33.0	15.0	37.0	7.0	40.0
40	27.31980309767606	33.0	15.0	38.0	7.0	40.0
41	26.660672568297095	33.0	15.0	37.0	7.0	40.0
42	27.189519846612	33.0	15.0	38.0	7.0	40.0
43	27.26915786181482	33.0	15.0	38.0	7.0	40.0
44	27.229490474442134	33.0	15.0	38.0	7.0	40.0
45	27.363010103482505	33.0	15.0	38.0	7.0	40.0
46	27.13217340458804	33.0	15.0	38.0	7.0	40.0
47	27.194643658466937	33.0	15.0	38.0	7.0	40.0
48	26.89725723765621	33.0	14.0	37.0	7.0	40.0
49	26.70484776785107	33.0	12.0	37.0	7.0	40.0
50	26.886971538911325	33.0	12.0	37.0	7.0	40.0
51	25.582292389072464	31.0	11.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	20.0
10	35.0
11	38.0
12	58.0
13	89.0
14	179.0
15	314.0
16	697.0
17	1436.0
18	2891.0
19	4864.0
20	7734.0
21	10972.0
22	15807.0
23	21763.0
24	30287.0
25	40674.0
26	48624.0
27	46593.0
28	40964.0
29	39672.0
30	41997.0
31	47376.0
32	54220.0
33	60886.0
34	66197.0
35	67608.0
36	50309.0
37	27311.0
38	5665.0
39	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.811011844055535	2.160758237125879	30.310039570839965	45.71819034797862
2	46.247841281496896	12.28395817185439	28.924108296278167	12.54409225037055
3	10.38972517983655	12.244659296427745	64.496253688519	12.869361835216688
4	9.416908035192211	2.4204843688383035	73.17287425719687	14.989733338772623
5	16.992344198316538	2.940480561334802	64.76291491589497	15.304260324453692
6	11.924421055494363	10.289234283849385	67.38560491711881	10.400739743537443
7	46.07364799630128	1.0584859734290648	50.21933939814248	2.6485266321271705
8	46.515182420212405	7.4901752811433395	42.309930785025635	3.684711513618624
9	42.73745903534179	2.3539890398292065	42.15232733651531	12.756224588313684
10	22.448224751492404	15.378914589537523	49.427922598893105	12.744938060076965
11	18.157304287520905	15.385169773861488	53.700349474428535	12.757176464189069
12	14.260732400494975	13.655339343749576	56.48472239220006	15.599205863555394
13	13.874542759624145	14.38841975006459	61.03305728932947	10.70398020098179
14	13.133575381770218	17.13594147323189	54.959409293028195	14.771073851969705
15	10.72437754116863	15.29338174302071	60.885516528644665	13.096724187165993
16	11.849222861338882	15.347502685649792	54.165680795224304	18.637593657787026
17	12.126898652415726	16.189776853098355	57.39498769360475	14.288336800881165
18	12.175308339792492	15.293245760752797	56.271910142917356	16.259535756537346
19	12.436394294184039	18.025945416717658	53.24997620310311	16.287684085995185
20	13.967282666340308	17.035314594976814	57.49343885557323	11.503963883109643
21	14.421735405703096	19.29615578128612	53.86896748663974	12.413141326371042
22	12.75364092522335	14.672894654537046	55.716694543031586	16.85676987720801
23	12.910836426929928	19.304994628700417	54.644066413739644	13.140102530630005
24	13.712995825344375	15.83023973673833	53.767116767973455	16.689647669943838
25	11.739349188865772	20.73933559063898	52.19597764451515	15.325337575980091
26	12.297420416377705	16.239682345422157	55.1946586165164	16.268238621683732
27	13.762765335400266	17.076653204422144	52.296332558234404	16.86424890194319
28	11.135587919335318	17.42830334924326	55.479405485524694	15.956703245896737
29	14.070085260881982	14.583146357714954	52.226165707991676	19.120602673411387
30	11.90796720107698	18.346319639918953	52.72794027658794	17.017772882416132
31	13.254055671140483	18.315587647370783	50.11830457308367	18.312052108405062
32	13.825589143175728	18.72856579502033	49.30417873509295	18.141666326710997
33	12.2630169025959	18.03383238825657	46.949373801656265	22.753776907491265
34	14.412216646949238	21.90973497055984	44.48795877017637	19.190089612314555
35	12.65274208243245	20.85301676661363	44.19600484096874	22.298236309985178
36	14.398754402425922	22.76071200315479	42.11452426603571	20.72600932838358
37	14.637539264879859	22.301091937611336	41.61138987476033	21.449978922748475
38	16.12219366594596	23.49827982431091	40.038211017283345	20.34131549245978
39	17.41456914018412	23.715443506166796	39.40262989706142	19.46735745658766
40	16.932512000435143	21.692027359632306	42.561769945199146	18.813690694733406
41	16.270822284774063	25.109533716803327	41.17148723806416	17.44815676035845
42	16.667346578006228	22.57468826065081	43.33754878363861	17.42041637770435
43	18.075035015433986	21.164144195596894	40.12931913678457	20.631501652184557
44	18.437155794884347	22.435714382844477	38.04049551938427	21.086634302886903
45	16.536803600810455	21.979221909463007	39.066345748514394	22.417628741212145
46	19.32240035899319	23.009015624362583	37.73834292008323	19.93024109656101
47	15.628849997960264	20.683310896259126	43.527787976447875	20.160051129332736
48	17.741062565441467	22.788180421273065	37.575572145392236	21.895184867893228
49	15.872666204326954	20.785841526264974	41.7640979616258	21.577394307782267
50	16.92149743673425	20.006935095663525	40.69174179686969	22.379825670732536
51	17.341682644583145	20.268700961394632	38.342648118685325	24.046968275336898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	535.0
2	974.0
3	31761.5
4	62549.0
5	44406.5
6	26264.0
7	25301.5
8	24339.0
9	24160.5
10	23982.0
11	23245.0
12	22508.0
13	21705.5
14	20903.0
15	19827.0
16	18751.0
17	17713.0
18	16675.0
19	15566.0
20	14457.0
21	13355.5
22	12254.0
23	11566.5
24	10879.0
25	10341.0
26	9601.5
27	9400.0
28	9697.5
29	9995.0
30	10453.0
31	10911.0
32	11961.5
33	13012.0
34	14302.5
35	15593.0
36	16684.0
37	17775.0
38	18734.0
39	19693.0
40	21181.5
41	22670.0
42	24385.0
43	26100.0
44	28744.5
45	31389.0
46	55141.5
47	78894.0
48	66188.0
49	53482.0
50	48220.0
51	42958.0
52	36710.0
53	30462.0
54	26992.0
55	23522.0
56	21137.0
57	18752.0
58	16363.5
59	13975.0
60	12297.0
61	10619.0
62	9098.5
63	7578.0
64	6402.5
65	5227.0
66	4196.5
67	3166.0
68	2660.5
69	2155.0
70	1700.5
71	1246.0
72	1052.0
73	858.0
74	789.0
75	560.0
76	400.0
77	297.5
78	195.0
79	154.5
80	114.0
81	83.0
82	52.0
83	38.0
84	24.0
85	16.0
86	8.0
87	6.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	735390.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.214435941788494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.44931304240825	43.874936873397004
2	7.459830860922835	7.939413829703101
3	3.4751565635089845	5.547854890096041
4	2.09631613851843	4.462171234677056
5	1.337538574211467	3.5588180388523614
6	0.9356304717238649	2.987342868164098
7	0.6287235117276876	2.3420016927950535
8	0.4090102801941945	1.741220108394154
9	0.2975363342611564	1.4249905379905388
>10	0.8236998719990711	6.652999862713567
>50	0.044012853382610566	1.6717735832402287
>100	0.03567900667698107	3.807531848645341
>500	0.004166891290720413	1.4666633746124444
>1k	0.002343876351030232	2.4549253536695494
>5k	2.604307056700258E-4	1.1207508939328015
>10k+	7.812921170100776E-4	8.946605009116599
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40040	5.444730007207061	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	13767	1.872067882348142	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	10749	1.4616733977889282	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	8087	1.099688600606481	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	3534	0.4805613348019418	RNA PCR Primer, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	2803	0.3811582969580767	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTT	2502	0.3402276343164851	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTC	2297	0.31235126939447094	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGC	1517	0.20628510042290488	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTA	1381	0.1877915119868369	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	1331	0.1809923985912237	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	1181	0.16059505840438407	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCCACCATTTCGTATGCCG	1168	0.15882728892152462	No Hit
GAATCTTTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	913	0.12415181060389725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	862	0.11721671494037177	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACCATTTCGT	813	0.11055358381267084	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGCCG	757	0.10293857680958403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	748	0.10171473639837365	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10089884279090007	0.0
2	0.0	0.0	0.0	0.652034974639307	0.0
3	0.0	0.0	0.0	0.840914344769442	0.0
4	0.0	0.0	0.0	1.691483430560655	0.0
5	0.0	0.0	0.0	3.7037490311263412	0.0
6	0.0	0.0	0.0	4.296223772420077	0.0
7	0.0	0.0	0.0	4.849671602822992	0.0
8	0.0	0.0	0.0	5.550116264839065	0.0
9	0.0	0.0	0.0	5.826840180040523	0.0
10	0.0	0.0	0.0	8.44640258910238	0.0
11	0.0	0.0	0.0	8.981628795605053	0.0
12	0.0	0.0	0.0	10.930798623859449	0.0
13	0.0	0.0	0.0	11.198275744842872	0.0
14	0.0	0.0	0.0	11.357918927371871	0.0
15	0.0	0.0	0.0	11.765321802037015	0.0
16	0.0	0.0	0.0	12.028175525911422	0.0
17	0.0	0.0	0.0	12.257033682807762	0.0
18	0.0	0.0	0.0	12.468486109411332	0.0
19	1.3598226791226425E-4	0.0	0.0	13.107058839527326	0.0
20	1.3598226791226425E-4	0.0	0.0	13.328981900760141	0.0
21	1.3598226791226425E-4	0.0	0.0	13.560423720746815	0.0
22	1.3598226791226425E-4	0.0	0.0	13.888956880022844	0.0
23	1.3598226791226425E-4	0.0	0.0	14.143787650090427	0.0
24	1.3598226791226425E-4	0.0	0.0	14.356327934837298	0.0
25	1.3598226791226425E-4	0.0	0.0	14.535688546213574	0.0
26	1.3598226791226425E-4	0.0	0.0	14.704034593888958	0.0
27	1.3598226791226425E-4	0.0	0.0	14.875644215994233	0.0
28	1.3598226791226425E-4	0.0	0.0	15.038279008417302	0.0
29	1.3598226791226425E-4	0.0	0.0	15.223622839581719	0.0
30	1.3598226791226425E-4	0.0	0.0	15.425284542895607	0.0
31	1.3598226791226425E-4	0.0	0.0	15.60804471096969	0.0
32	2.719645358245285E-4	0.0	0.0	15.799507744190157	0.0
33	2.719645358245285E-4	0.0	0.0	15.98648336256952	0.0
34	2.719645358245285E-4	0.0	0.0	16.177946395789988	0.0
35	4.0794680373679274E-4	0.0	0.0	16.373080950244088	0.0
36	4.0794680373679274E-4	0.0	0.0	16.565767823875767	0.0
37	4.0794680373679274E-4	0.0	0.0	16.766613633582182	0.0
38	4.0794680373679274E-4	0.0	0.0	16.962564081643755	0.0
39	4.0794680373679274E-4	0.0	0.0	17.188974557717675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTTA	20	7.0316275E-4	45.0	18
TCTAGCG	20	7.0316275E-4	45.0	1
CGTTTTT	26215	0.0	44.46786	1
ACGGCTG	1195	0.0	42.17573	8
TGATACC	1845	0.0	41.95122	4
GATACCT	1840	0.0	41.942936	5
CTTCGCG	65	0.0	41.53846	1
ACCGGTC	65	0.0	41.53846	17
TACGGCT	1220	0.0	41.4959	7
CGACCAC	1000	0.0	41.4	12
GCGATTT	250	0.0	41.4	9
GAATGAT	5085	0.0	41.283184	1
GCGACCA	1025	0.0	41.26829	11
TTGCGCG	60	3.6379788E-12	41.249996	1
ACGGGAG	55	6.002665E-11	40.909092	5
CGATGAA	165	0.0	40.909092	19
ATGATAC	4895	0.0	40.817158	3
CGGGCGA	250	0.0	40.500004	6
AATGATA	4925	0.0	40.477158	2
ACGTGAG	245	0.0	40.408165	44
>>END_MODULE