##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406125
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.260690674053556	31.0	28.0	31.0	25.0	34.0
2	28.269862726992923	31.0	25.0	33.0	16.0	34.0
3	28.26486919052016	31.0	25.0	33.0	16.0	34.0
4	32.9812594644506	35.0	32.0	37.0	28.0	37.0
5	34.20638473376424	35.0	33.0	37.0	30.0	37.0
6	33.34443582640812	35.0	32.0	37.0	28.0	37.0
7	33.60893567251462	35.0	32.0	37.0	29.0	37.0
8	33.298917820867956	35.0	32.0	37.0	28.0	37.0
9	34.709572176054174	37.0	34.0	39.0	28.0	39.0
10	34.76066728224069	37.0	33.0	39.0	28.0	39.0
11	35.00933702677747	37.0	34.0	39.0	28.0	39.0
12	35.08409233610342	37.0	34.0	39.0	27.0	39.0
13	35.134160664819944	37.0	34.0	39.0	27.0	39.0
14	35.92031271160357	38.0	34.0	40.0	27.0	41.0
15	36.17518990458603	38.0	34.0	40.0	28.0	41.0
16	36.22395814096645	38.0	34.0	40.0	30.0	41.0
17	35.401019390581716	37.0	33.0	40.0	27.0	41.0
18	35.17806586642044	37.0	33.0	39.0	27.0	40.0
19	34.54103047091413	37.0	33.0	38.0	27.0	40.0
20	33.710763927362265	35.0	32.0	38.0	26.0	40.0
21	34.276072637734686	35.0	33.0	38.0	27.0	40.0
22	34.75489073561096	35.0	33.0	39.0	29.0	40.0
23	34.981714989227456	35.0	33.0	39.0	29.0	40.0
24	34.554287473068634	35.0	33.0	39.0	27.0	40.0
25	33.52566081871345	35.0	33.0	38.0	24.0	40.0
26	33.55125884887658	35.0	32.0	38.0	24.0	40.0
27	34.27846106494306	35.0	33.0	38.0	26.0	40.0
28	33.89001662049861	35.0	33.0	39.0	24.0	40.0
29	33.86032379193598	35.0	33.0	39.0	24.0	40.0
30	33.20790889504463	35.0	32.0	39.0	21.0	40.0
31	33.31162080640197	35.0	33.0	39.0	22.0	40.0
32	32.72826592797784	35.0	32.0	39.0	18.0	40.0
33	31.980419821483533	35.0	31.0	39.0	15.0	40.0
34	31.60855647891659	35.0	30.0	39.0	10.0	40.0
35	31.050976915974147	35.0	27.0	39.0	8.0	41.0
36	30.469564789165897	35.0	24.0	39.0	7.0	40.0
37	30.370425361649737	35.0	23.0	39.0	7.0	40.0
38	30.069980917205292	35.0	23.0	39.0	7.0	40.0
39	29.765030470914127	35.0	22.0	39.0	7.0	40.0
40	29.411063096337333	35.0	21.0	39.0	7.0	40.0
41	29.004510926438904	35.0	21.0	39.0	7.0	40.0
42	29.34219267466913	35.0	21.0	39.0	7.0	40.0
43	29.06934810710988	35.0	20.0	39.0	7.0	40.0
44	29.216046783625732	35.0	20.0	39.0	7.0	40.0
45	29.347353647276083	35.0	20.0	39.0	7.0	40.0
46	29.062980609418283	35.0	20.0	39.0	7.0	40.0
47	28.865324715297014	35.0	20.0	39.0	7.0	40.0
48	28.75977593105571	34.0	20.0	38.0	7.0	40.0
49	28.995636811326563	35.0	20.0	39.0	7.0	40.0
50	28.842755309325945	34.0	20.0	39.0	7.0	40.0
51	27.76314188981225	33.0	19.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	18.0
10	27.0
11	36.0
12	42.0
13	53.0
14	72.0
15	141.0
16	190.0
17	432.0
18	902.0
19	1596.0
20	2562.0
21	3834.0
22	5288.0
23	7453.0
24	10642.0
25	15404.0
26	20293.0
27	22067.0
28	20673.0
29	19261.0
30	19326.0
31	21187.0
32	24068.0
33	28292.0
34	31874.0
35	36060.0
36	38768.0
37	40189.0
38	30605.0
39	4762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.074730686365037	2.4022160664819943	28.90366266543552	38.61939058171745
2	37.514804555247764	20.122376115727917	30.207448445675595	12.155370883348722
3	11.092151431209603	20.27774699907664	56.28513388734996	12.344967682363803
4	10.398030163127116	2.5442905509387503	72.81354262850108	14.244136657433057
5	24.182948599569098	3.197783933518006	56.48654970760234	16.13271775931056
6	12.16448137888581	17.980424746075716	59.99926131117267	9.855832563865805
7	48.68525700215451	1.1176361957525394	47.54349030470914	2.6536164973838106
8	47.15370883348723	14.199322868574946	35.21108033240997	3.4358879655278547
9	44.23859649122807	3.0170514004309017	34.13850415512466	18.605847953216376
10	27.07910126192675	19.598153277931672	40.42351492767005	12.899230532471528
11	24.749276700523236	14.929147429978453	47.362757771622036	12.958818097876271
12	18.794952293013235	13.243213296398892	48.5513080947984	19.410526315789472
13	17.275961834410587	14.472883964296706	56.99649122807018	11.25466297322253
14	14.943428747306864	21.715235457063713	46.4563865804863	16.884949215143124
15	10.5689135118498	15.188180978762697	58.86044936903663	15.382456140350879
16	12.331917513080947	16.573961218836565	47.65478608802709	23.4393351800554
17	13.129947676208065	17.27546937519237	53.580547860880266	16.014035087719297
18	12.48654970760234	16.446168051708216	51.53265620190828	19.534626038781163
19	13.04327485380117	19.21132656201908	47.91997537703909	19.82542320714066
20	16.182948599569098	17.425423207140657	54.07793167128347	12.313696522006772
21	16.081009541397354	23.763127116035704	46.98504155124654	13.170821791320405
22	13.613296398891967	14.656325023084026	51.23644198214835	20.493936595875653
23	14.523361034164358	23.30637119113573	48.617297630040014	13.552970144659895
24	16.082240689442905	17.061495844875346	46.72403816558941	20.132225300092337
25	12.548845798707294	26.98528778085565	45.05435518621114	15.411511234225916
26	13.780978762696213	16.28267159125885	50.79495229301324	19.141397353031703
27	16.50698676515851	20.340781779008925	44.86081871345029	18.29141274238227
28	11.625238534933825	19.46346568174823	52.19673745767929	16.71455832563866
29	20.220621729763007	15.957402277623883	45.752908587257615	18.069067405355494
30	13.226962142197598	25.529332102185286	47.182271468144044	14.061434287473068
31	17.211203447214526	16.344721452754694	43.20492459218221	23.239150507848567
32	18.935549399815326	22.758756540473993	41.804616805170824	16.501077254539858
33	15.640012311480456	21.094736842105263	40.05564789165897	23.209602954755308
34	22.202277623884275	19.45410895660203	42.51178824253616	15.831825176977532
35	15.128100954139736	24.78473376423515	40.807879347491536	19.27928593413358
36	22.06734379809172	21.23681132656202	35.97980917205294	20.71603570329332
37	15.33985841797476	25.31905201600492	40.05564789165897	19.28544167436134
38	17.746752847029857	25.59507540781779	34.54847645429363	22.109695290858724
39	19.3243459526008	21.979193598030164	38.91388119421361	19.782579255155433
40	16.469806094182825	25.201108033241	41.25601723607264	17.07306863650354
41	20.46734379809172	24.10907971683595	37.69652200677132	17.727054478301017
42	16.03939673745768	22.289935364727608	43.67473068636504	17.99593721144968
43	23.063835026161897	21.85952600800246	33.80215450907972	21.274484456755925
44	19.535857186826718	23.800307787011388	36.88938134810711	19.774453678054783
45	16.571006463527237	21.274730686365036	37.229916897506925	24.924345952600802
46	22.59353647276085	24.91991381963681	33.65490920283164	18.8316405047707
47	15.243582640812559	22.209664512157588	44.25189289012004	18.294859956909818
48	19.54866112650046	23.740720221606647	34.791751308094796	21.918867343798095
49	16.143059402893197	20.2440135426285	42.590089258233306	21.022837796244996
50	20.111049553708835	19.804001231148046	37.715727916281935	22.369221298861188
51	18.468451831332718	20.19033548784241	36.13518005540166	25.20603262542321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	678.0
2	1304.0
3	14733.0
4	28162.0
5	21296.0
6	14430.0
7	13601.0
8	12772.0
9	12380.5
10	11989.0
11	11279.0
12	10569.0
13	9805.5
14	9042.0
15	8376.5
16	7711.0
17	6954.5
18	6198.0
19	5821.5
20	5445.0
21	5045.5
22	4646.0
23	4505.0
24	4364.0
25	4181.5
26	4258.0
27	4517.0
28	4959.0
29	5401.0
30	5648.5
31	5896.0
32	6535.5
33	7175.0
34	7568.0
35	7961.0
36	8746.0
37	9531.0
38	10044.0
39	10557.0
40	10956.5
41	11356.0
42	12092.5
43	12829.0
44	14592.0
45	16355.0
46	42733.0
47	69111.0
48	47436.5
49	25762.0
50	23139.5
51	20517.0
52	17469.5
53	14422.0
54	13621.5
55	12821.0
56	12269.5
57	11718.0
58	10875.5
59	10033.0
60	9488.0
61	8943.0
62	7834.0
63	6725.0
64	5682.0
65	4639.0
66	3934.5
67	3230.0
68	2571.5
69	1913.0
70	1692.0
71	1471.0
72	1224.0
73	977.0
74	836.0
75	559.5
76	424.0
77	345.0
78	266.0
79	181.0
80	96.0
81	76.5
82	57.0
83	45.5
84	34.0
85	20.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.80929557116283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63798223464282	35.3865441579501
2	5.115472111601742	4.2774857099999535
3	1.9595740568765032	2.4578523281259708
4	1.169290102467714	1.955487820100317
5	0.924263564592246	1.9321404278846883
6	0.7743872473340319	1.9425951186196628
7	0.656888970280742	1.9224845581133034
8	0.5731343560426219	1.9169874955019242
9	0.56237261137156	2.1161162456964213
>10	3.497761213049439	23.682727967319227
>50	0.08195643954010587	2.270952465863543
>100	0.03860267079787595	2.9003931504211726
>500	0.002375548972176982	0.6781074988271744
>1k	0.003563323458265473	3.7664638043608227
>5k	0.0	0.0
>10k+	0.002375548972176982	12.79366125121573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	14908	3.6707910126192678	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13133	3.233733456448138	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	12634	3.1108648815020006	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	10850	2.671591258848877	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT	3061	0.7537088334872268	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC	2600	0.6401969836872884	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA	2432	0.5988304093567252	No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	2411	0.5936595875654048	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT	2348	0.5781471221914436	No Hit
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	2317	0.5705140043090182	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACCATTGTCGT	915	0.2253000923361034	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTT	644	0.15857186826715913	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTAT	602	0.14823022468451832	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATG	570	0.14035087719298245	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	438	0.10784856879039705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08322560787934749	0.0
2	0.0	0.0	0.0	0.7916281932902431	0.0
3	0.0	0.0	0.0	0.8854416743613419	0.0
4	0.0	0.0	0.0	1.5857186826715912	0.0
5	0.0	0.0	0.0	4.7807940904893815	0.0
6	0.0	0.0	0.0	5.01865189289012	0.0
7	0.0	0.0	0.0	5.738627269929209	0.0
8	0.0	0.0	0.0	6.539612188365651	0.0
9	0.0	0.0	0.0	6.645490920283164	0.0
10	0.0	0.0	0.0	11.02123730378578	0.0
11	0.0	0.0	0.0	11.216497383810403	0.0
12	0.0	0.0	0.0	15.07146814404432	0.0
13	0.0	0.0	0.0	15.34453678054786	0.0
14	0.0	0.0	0.0	15.559002770083103	0.0
15	0.0	0.0	0.0	16.348414896891352	0.0
16	0.0	0.0	0.0	16.69978454909203	0.0
17	0.0	0.0	0.0	16.86549707602339	0.0
18	0.0	0.0	0.0	16.982456140350877	0.0
19	2.4622960911049554E-4	0.0	0.0	18.015389350569407	0.0
20	2.4622960911049554E-4	0.0	0.0	18.229362880886427	0.0
21	2.4622960911049554E-4	0.0	0.0	18.361588180978764	0.0
22	2.4622960911049554E-4	0.0	0.0	18.642536164973837	0.0
23	2.4622960911049554E-4	0.0	0.0	18.772545398584178	0.0
24	2.4622960911049554E-4	0.0	0.0	18.87817790089258	0.0
25	2.4622960911049554E-4	0.0	0.0	18.964358264081255	0.0
26	2.4622960911049554E-4	0.0	0.0	19.08624192059095	0.0
27	2.4622960911049554E-4	0.0	0.0	19.253185595567867	0.0
28	2.4622960911049554E-4	0.0	0.0	19.33985841797476	0.0
29	2.4622960911049554E-4	0.0	0.0	19.450661742074484	0.0
30	2.4622960911049554E-4	0.0	0.0	19.567374576792858	0.0
31	2.4622960911049554E-4	0.0	0.0	19.68064019698369	0.0
32	2.4622960911049554E-4	0.0	0.0	19.804493690366268	0.0
33	2.4622960911049554E-4	0.0	0.0	19.938935056940597	0.0
34	2.4622960911049554E-4	0.0	0.0	20.136903662665436	0.0
35	2.4622960911049554E-4	0.0	0.0	20.270852570021546	0.0
36	2.4622960911049554E-4	0.0	0.0	20.390027700831023	0.0
37	2.4622960911049554E-4	0.0	0.0	20.540227762388426	0.0
38	2.4622960911049554E-4	0.0	0.0	20.68082486919052	0.0
39	2.4622960911049554E-4	0.0	0.0	20.86451215758695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	7.027892E-4	45.000004	5
GCGAACC	30	2.1620217E-6	45.000004	33
TCGGGCT	35	1.2093369E-7	45.000004	5
CGCGCGA	20	7.027892E-4	45.000004	41
TTATACG	20	7.027892E-4	45.000004	26
ATATCAC	30	2.1620217E-6	45.000004	30
CGAGGGA	20	7.027892E-4	45.000004	4
ACTACCG	20	7.027892E-4	45.000004	14
CTACCGA	20	7.027892E-4	45.000004	15
CTTCTCG	35	1.2093369E-7	45.000004	1
ACATCGA	20	7.027892E-4	45.000004	21
CACGTCT	20	7.027892E-4	45.000004	40
CACCGAA	20	7.027892E-4	45.000004	16
GCGACTA	25	3.886335E-5	45.0	11
CGTTTTT	11015	0.0	44.26464	1
GCGACTG	335	0.0	43.656715	11
GGCGACT	365	0.0	43.150684	10
CGATGAA	445	0.0	42.977528	19
TTGATAC	110	0.0	42.954544	3
ACGGCTG	1485	0.0	42.878784	8
>>END_MODULE