FastQCFastQC Report
Sat 14 Jan 2017
SRR2933453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267591
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191417.153080634251525No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG77132.882383936679485No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC54692.043790710449903No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC35401.3229144477953294No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT15100.5642940158674993No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC14600.5456087835540059TruSeq Adapter, Index 27 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT10380.387905422828122No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA9950.37183612303851776No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC9270.34642420709216676No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT6380.2384235643201752No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGACCATTGTCGTATGCCG6310.2358076317962861No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACCATTGTCGT3590.13415996801088229No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT3530.13191774013326307TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCCTTGTCGTATGC3450.12892810296310414No Hit
GAATCTTTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC3210.11995919145262733No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3200.11958548680635747No Hit
GAATGACTCTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT2960.11061657529588065No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGACCATTGTCGTATGC2850.10650582418691211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGC453.8198777E-1045.0000046
CTTCGTG207.0235756E-445.01
ACCGCTG351.2072633E-745.017
GACCGAT351.2072633E-745.09
TTATCGG207.0235756E-445.02
ACTAATC551.8189894E-1245.010
GGACCGA406.7811925E-945.08
GGATTGA207.0235756E-445.08
CACCGCT406.7811925E-945.016
TATCGGG207.0235756E-445.03
GCGAGAC302.1592386E-644.99999621
TCGTTCG253.8827595E-544.9999961
TTATACG253.8827595E-544.99999626
TCGCTTG253.8827595E-544.9999961
ACGTGAG302.1592386E-644.99999644
AGACACG302.1592386E-644.99999624
TATACGG253.8827595E-544.99999627
AACACGT302.1592386E-644.99999641
CGTTTTT81250.044.418461
CCGATGA1100.042.95454418
Produced by FastQC (version 0.11.5)