##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933453.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267591 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.16466547828589 31.0 26.0 31.0 16.0 33.0 2 28.191176085892277 31.0 28.0 33.0 16.0 34.0 3 28.36168630484583 31.0 28.0 33.0 16.0 34.0 4 31.695083167969027 35.0 28.0 37.0 19.0 37.0 5 33.608678916704974 35.0 33.0 37.0 28.0 37.0 6 32.131338497931544 35.0 32.0 37.0 17.0 37.0 7 32.2245553848971 35.0 32.0 35.0 26.0 37.0 8 32.499381518810424 35.0 32.0 35.0 25.0 37.0 9 33.04615252381433 35.0 32.0 37.0 25.0 39.0 10 32.165644584459116 35.0 28.0 39.0 17.0 39.0 11 32.68527715805091 37.0 30.0 39.0 17.0 39.0 12 33.481077465236126 35.0 31.0 39.0 25.0 39.0 13 33.547727688898355 35.0 32.0 39.0 25.0 39.0 14 33.62943073571234 36.0 32.0 40.0 20.0 41.0 15 34.147194786072774 37.0 32.0 40.0 25.0 41.0 16 34.40213609575808 37.0 32.0 40.0 25.0 41.0 17 32.33057539304386 35.0 27.0 39.0 18.0 41.0 18 32.995537966523536 36.0 30.0 39.0 23.0 39.0 19 32.7992084935592 36.0 30.0 37.0 21.0 39.0 20 32.246290047124155 34.0 30.0 36.0 24.0 39.0 21 32.75641183746838 35.0 31.0 37.0 24.0 39.0 22 32.92382778195081 35.0 31.0 37.0 25.0 39.0 23 33.8252669185436 35.0 32.0 38.0 27.0 40.0 24 32.2922407704295 35.0 31.0 37.0 21.0 39.0 25 31.38750182181015 34.0 29.0 37.0 18.0 39.0 26 30.573614209745468 33.0 25.0 37.0 18.0 39.0 27 32.364309711462646 34.0 30.0 37.0 22.0 39.0 28 32.25226558441801 35.0 31.0 37.0 21.0 39.0 29 32.344439835420474 35.0 30.0 38.0 21.0 40.0 30 31.35119267837857 34.0 29.0 37.0 18.0 40.0 31 31.82367493675049 35.0 30.0 37.0 21.0 39.0 32 31.75705460945996 35.0 30.0 37.0 18.0 40.0 33 31.553710700285137 35.0 30.0 38.0 15.0 40.0 34 30.831661005041276 35.0 29.0 38.0 12.0 40.0 35 30.241140397098558 35.0 26.0 38.0 10.0 40.0 36 29.57505297263361 35.0 24.0 38.0 8.0 40.0 37 29.571603678748538 35.0 23.0 38.0 8.0 40.0 38 29.331935677956285 35.0 23.0 38.0 8.0 40.0 39 29.02757192880179 34.0 23.0 38.0 7.0 40.0 40 28.658673124282956 34.0 22.0 37.0 7.0 40.0 41 28.22099397961815 33.0 21.0 37.0 7.0 40.0 42 28.809519004749784 34.0 22.0 38.0 7.0 40.0 43 28.670444820640455 34.0 21.0 38.0 7.0 40.0 44 28.610248476219304 34.0 21.0 38.0 7.0 40.0 45 28.87274983089865 34.0 21.0 38.0 7.0 40.0 46 28.553998452862764 34.0 20.0 38.0 7.0 40.0 47 28.473255826989696 34.0 20.0 38.0 7.0 40.0 48 28.279209689414067 34.0 20.0 37.0 7.0 40.0 49 28.160308082110387 33.0 20.0 37.0 7.0 40.0 50 28.26083836900344 34.0 20.0 37.0 7.0 40.0 51 26.74176635238106 31.0 18.0 35.0 7.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 14.0 10 6.0 11 16.0 12 23.0 13 32.0 14 55.0 15 120.0 16 267.0 17 574.0 18 933.0 19 1515.0 20 2333.0 21 3292.0 22 4481.0 23 6202.0 24 8492.0 25 11337.0 26 14470.0 27 16020.0 28 15913.0 29 15779.0 30 17182.0 31 19668.0 32 21980.0 33 23576.0 34 25665.0 35 26986.0 36 18722.0 37 9806.0 38 2075.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.994147785239413 2.3973153058211976 30.98646815475857 40.62206875418082 2 41.128812254522764 15.588341909854966 30.98086258506452 12.301983250557754 3 11.313908165820227 15.499400204042738 60.8596701682792 12.327021461857836 4 10.96299950297282 2.4937311045588233 72.08575774222601 14.457511650242347 5 21.39272247571854 3.075589238801006 60.112260875739466 15.419427409740985 6 12.419326509486492 13.953384082424297 62.61010273140726 11.017186676681952 7 48.306931100074365 1.0859857020602337 47.89062412412973 2.7164590737356638 8 48.330848197435635 11.13789327742712 36.5371032658049 3.9941552593323397 9 44.66966377792975 2.870799092645119 36.49151129895998 15.968025830465148 10 24.902556513485134 19.90238834639431 42.158368554996244 13.036686585124313 11 22.51944198422219 15.477351629912814 49.77185331345225 12.23135307241275 12 18.465867686132942 13.818103000474604 50.32643100851673 17.389598304875726 13 16.340983067442476 14.934732483528968 57.56172666494762 11.16255778408093 14 14.150326431008518 18.944957042650913 49.26884685957301 17.63586966676757 15 10.329196422899125 15.667940999510446 58.72021106838421 15.282651509206213 16 11.999282487079162 15.030774577620324 50.092491899951796 22.877451035348724 17 12.779577788490645 16.128345123714922 54.948783778228716 16.143293309565717 18 11.941731971553603 15.189225347638747 53.80861090245935 19.0604317783483 19 13.111427514378285 18.289852797739833 49.73597766741034 18.86274202047154 20 15.51584320847861 16.595849636198526 55.529894503178355 12.358412652144505 21 16.141051081688097 21.41626586843354 48.45865518645993 13.984027863418424 22 13.315843955887905 14.788987671483719 52.231577295200516 19.663591077427867 23 13.987391205234855 22.13564731250304 49.62050293171295 14.256458550549159 24 14.98555631542167 16.098448752013333 49.34171926559563 19.574275666969367 25 12.605805127975156 23.063929653837388 47.39957621893113 16.930688999256326 26 13.484384751355613 16.683296523425675 52.188974965525745 17.643343759692964 27 14.245247411161063 19.777197289893905 47.98703992286736 17.99051537607767 28 11.488801940274524 18.46848361865683 53.85943473435205 16.183279706716593 29 18.001726515465766 15.69372662010307 48.314405192999764 17.9901416714314 30 12.894678819541763 23.03179105425818 49.48858519157969 14.584944934620372 31 15.907111973123161 16.582022564286543 46.53407625817012 20.97678920442018 32 17.307009578050085 21.54033581099514 45.445474623585994 15.707179987368782 33 14.913057614045316 19.360516609303 43.98316834273201 21.743257433919677 34 20.526848810311257 18.91692919418067 44.80830820169587 15.747913793812199 35 14.316998703244877 23.821802676472675 42.88597150128368 18.97522711899877 36 19.73496866486541 20.239096232683462 39.4280824093486 20.597852693102535 37 14.745264227870145 24.143936081557303 41.93750910905075 19.1732905815218 38 16.74421038076766 24.137209397924444 38.44187584784242 20.676704373465476 39 17.5151630660224 21.11057546778479 42.34484717348491 19.0294142927079 40 16.067431266372935 23.33075477127407 43.75857185032381 16.843242112029177 41 19.325388372553636 21.828835797915474 41.302584915038246 17.54319091449264 42 15.794626874595933 20.91737016566327 45.28889237679892 17.999110582941878 43 21.370673901588617 20.68716810356103 37.43773146331528 20.504426531535067 44 18.86274202047154 22.615484078313546 39.58765429330583 18.934119607909086 45 16.263626205664615 20.52049583132467 39.75395286089592 23.461925102114794 46 20.231248435111794 23.86291018756236 36.903707523795646 19.0021338535302 47 14.87381862618698 21.10609101202955 45.52843705505791 18.49165330672556 48 18.50398556005247 22.1001453711074 38.29276769398074 21.103101374859392 49 15.466140490524719 19.103407812669335 43.9820472287932 21.44840446801275 50 19.085843694294653 18.86797388551932 39.90605065192776 22.140131768258275 51 17.604478476480896 19.26073746874895 39.09698009275349 24.03780396201666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89.0 1 281.0 2 473.0 3 14053.5 4 27634.0 5 18647.5 6 9661.0 7 9199.5 8 8738.0 9 8398.0 10 8058.0 11 7476.5 12 6895.0 13 6254.0 14 5613.0 15 5165.0 16 4717.0 17 4188.0 18 3659.0 19 3482.5 20 3306.0 21 2962.5 22 2619.0 23 2583.5 24 2548.0 25 2525.0 26 2588.5 27 2675.0 28 2927.5 29 3180.0 30 3354.0 31 3528.0 32 3943.0 33 4358.0 34 4620.0 35 4882.0 36 5424.0 37 5966.0 38 6200.0 39 6434.0 40 6924.0 41 7414.0 42 8057.0 43 8700.0 44 9931.5 45 11163.0 46 22535.0 47 33907.0 48 27232.0 49 20557.0 50 18298.0 51 16039.0 52 13524.5 53 11010.0 54 9903.5 55 8797.0 56 8074.0 57 7351.0 58 6838.5 59 6326.0 60 5943.5 61 5561.0 62 4837.0 63 4113.0 64 3547.0 65 2981.0 66 2532.0 67 2083.0 68 1721.5 69 1360.0 70 1178.5 71 997.0 72 813.0 73 629.0 74 541.0 75 370.0 76 287.0 77 217.5 78 148.0 79 119.0 80 90.0 81 75.5 82 61.0 83 41.0 84 21.0 85 13.5 86 6.0 87 3.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 267591.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.32475965809247 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97556338024062 31.846706121706518 2 7.509581705941662 6.0564415484981104 3 3.0806581726264213 3.7268040119969634 4 2.086692661697447 3.3658152025301917 5 1.4993569087946554 3.0230603494422477 6 1.2378052614801278 2.9948519803625073 7 1.0103649365062743 2.851990626210556 8 0.798912541708156 2.577276498577373 9 0.672242930830928 2.4397231155847967 >10 2.9981630323045896 18.237590564469926 >50 0.07480446633727421 2.047581279401984 >100 0.044882679802364524 3.512782340266534 >500 0.003989571537987958 1.283408340713363 >1k 0.003989571537987958 3.035777548011427 >5k 0.001994785768993979 5.301751409364948 >10k+ 9.973928844969895E-4 7.698439062862575 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19141 7.153080634251525 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG 7713 2.882383936679485 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC 5469 2.043790710449903 No Hit GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC 3540 1.3229144477953294 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACCATTGT 1510 0.5642940158674993 No Hit GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC 1460 0.5456087835540059 TruSeq Adapter, Index 27 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT 1038 0.387905422828122 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTA 995 0.37183612303851776 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTC 927 0.34642420709216676 No Hit GAATGACTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT 638 0.2384235643201752 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCGACCATTGTCGTATGCCG 631 0.2358076317962861 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACCATTGTCGT 359 0.13415996801088229 No Hit CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT 353 0.13191774013326307 TruSeq Adapter, Index 20 (95% over 22bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCCTTGTCGTATGC 345 0.12892810296310414 No Hit GAATCTTTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC 321 0.11995919145262733 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 320 0.11958548680635747 No Hit GAATGACTCTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCT 296 0.11061657529588065 No Hit GAATGATACGGCTGCCTCCTATACACATCTGACGCGACCATTGTCGTATGC 285 0.10650582418691211 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15620854214080443 0.0 2 0.0 0.0 0.0 1.0265666633033248 0.0 3 0.0 0.0 0.0 1.2332253326905613 0.0 4 0.0 0.0 0.0 1.9529804814063254 0.0 5 0.0 0.0 0.0 3.7964655014555797 0.0 6 0.0 0.0 0.0 4.086460306960997 0.0 7 0.0 0.0 0.0 4.529674017437059 0.0 8 0.0 0.0 0.0 5.146286683782339 0.0 9 0.0 0.0 0.0 5.296889656229096 0.0 10 0.0 0.0 0.0 9.08363883688166 0.0 11 0.0 0.0 0.0 9.629621325081935 0.0 12 0.0 0.0 0.0 12.642801887955873 0.0 13 0.0 0.0 0.0 12.908132186807478 0.0 14 0.0 0.0 0.0 13.07330964045876 0.0 15 0.0 0.0 0.0 13.661520753687531 0.0 16 0.0 0.0 0.0 14.005702732902078 0.0 17 0.0 0.0 0.0 14.302050517394083 0.0 18 0.0 0.0 0.0 14.586439753205452 0.0 19 0.0 0.0 0.0 15.567788154310122 0.0 20 0.0 0.0 0.0 15.91047531493959 0.0 21 0.0 0.0 0.0 16.22139758063612 0.0 22 0.0 0.0 0.0 16.75467411086322 0.0 23 0.0 0.0 0.0 17.078676039179197 0.0 24 0.0 0.0 0.0 17.31224144309786 0.0 25 0.0 0.0 0.0 17.524879386825415 0.0 26 0.0 0.0 0.0 17.73938585378432 0.0 27 0.0 0.0 0.0 17.93483338378346 0.0 28 3.737046462698671E-4 0.0 0.0 18.110474567530296 0.0 29 3.737046462698671E-4 0.0 0.0 18.312648781162295 0.0 30 3.737046462698671E-4 0.0 0.0 18.535376750339136 0.0 31 3.737046462698671E-4 0.0 0.0 18.758104719515977 0.0 32 3.737046462698671E-4 0.0 0.0 18.965884502842023 0.0 33 3.737046462698671E-4 0.0 0.0 19.17740133263077 0.0 34 3.737046462698671E-4 0.0 0.0 19.389291867065783 0.0 35 3.737046462698671E-4 0.0 0.0 19.617999110582943 0.0 36 3.737046462698671E-4 0.0 0.0 19.813072935935814 0.0 37 3.737046462698671E-4 0.0 0.0 20.009267875227494 0.0 38 3.737046462698671E-4 0.0 0.0 20.216300249261 0.0 39 3.737046462698671E-4 0.0 0.0 20.426695965110934 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCGGC 45 3.8198777E-10 45.000004 6 CTTCGTG 20 7.0235756E-4 45.0 1 ACCGCTG 35 1.2072633E-7 45.0 17 GACCGAT 35 1.2072633E-7 45.0 9 TTATCGG 20 7.0235756E-4 45.0 2 ACTAATC 55 1.8189894E-12 45.0 10 GGACCGA 40 6.7811925E-9 45.0 8 GGATTGA 20 7.0235756E-4 45.0 8 CACCGCT 40 6.7811925E-9 45.0 16 TATCGGG 20 7.0235756E-4 45.0 3 GCGAGAC 30 2.1592386E-6 44.999996 21 TCGTTCG 25 3.8827595E-5 44.999996 1 TTATACG 25 3.8827595E-5 44.999996 26 TCGCTTG 25 3.8827595E-5 44.999996 1 ACGTGAG 30 2.1592386E-6 44.999996 44 AGACACG 30 2.1592386E-6 44.999996 24 TATACGG 25 3.8827595E-5 44.999996 27 AACACGT 30 2.1592386E-6 44.999996 41 CGTTTTT 8125 0.0 44.41846 1 CCGATGA 110 0.0 42.954544 18 >>END_MODULE