SRR2933452.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933452.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259509
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	11792	4.543965719878694	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	10429	4.0187430879083195	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	8886	3.4241586996982765	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7975	3.073111144507512	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT	2429	0.9359983661452974	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	2256	0.8693340115371722	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC	2138	0.8238635268911675	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	1877	0.723288980343649	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	1846	0.7113433445468172	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	1798	0.6928468762162392	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGT	839	0.3233028526948969	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT	536	0.20654389635812245	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	522	0.20114909309503717	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG	485	0.18689139875688318	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCAAAGG	335	0.12908993522382653	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	296	0.1140615547052318	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTCAAAGGT	273	0.10519866363016311	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	262	0.10095988963773897	Illumina Single End Adapter 1 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1589858E-6	45.000004	6
GATACGC	20	7.023182E-4	45.000004	5
GGGTAAC	20	7.023182E-4	45.000004	7
GGCCGGT	20	7.023182E-4	45.000004	27
CGACCCT	20	7.023182E-4	45.000004	12
TCGCTTG	40	6.7793735E-9	45.000004	1
CGCAGGC	20	7.023182E-4	45.000004	34
ACGTCCA	20	7.023182E-4	45.000004	29
TGTCGTG	20	7.023182E-4	45.000004	1
CACGCGT	25	3.8824335E-5	45.0	41
CGGTGAA	75	0.0	45.0	19
TCCGTCC	25	3.8824335E-5	45.0	29
CGTTTTT	7130	0.0	44.337307	1
TGATACC	1325	0.0	43.4717	4
GATACCT	1315	0.0	43.288975	5
GCGACTG	275	0.0	42.545456	11
ACCGGTG	90	0.0	42.5	17
CCGGTGA	85	0.0	42.35294	18
ATACCTG	1350	0.0	42.166668	6
ACACGCG	75	0.0	42.0	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content