##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933452.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259509
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0561290745215	31.0	28.0	31.0	25.0	34.0
2	27.823135228450653	31.0	25.0	33.0	16.0	34.0
3	27.786477540277986	31.0	25.0	33.0	16.0	34.0
4	32.804253417029855	35.0	32.0	37.0	28.0	37.0
5	34.1862247552108	35.0	33.0	37.0	30.0	37.0
6	33.196490295134275	35.0	32.0	37.0	28.0	37.0
7	33.70523180313592	35.0	33.0	37.0	30.0	37.0
8	33.126608325722806	35.0	32.0	37.0	27.0	37.0
9	34.80095102674667	37.0	34.0	39.0	29.0	39.0
10	34.75576184255652	37.0	33.0	39.0	28.0	39.0
11	35.07433268210351	37.0	34.0	39.0	29.0	39.0
12	35.06763156576458	37.0	34.0	39.0	27.0	39.0
13	35.16772058001842	37.0	34.0	39.0	29.0	39.0
14	35.96242904870351	38.0	34.0	40.0	27.0	41.0
15	36.13749812145244	38.0	34.0	40.0	27.0	41.0
16	36.26275389292857	38.0	34.0	40.0	30.0	41.0
17	35.32895583582843	37.0	33.0	40.0	27.0	41.0
18	35.1467039678778	37.0	33.0	39.0	27.0	40.0
19	34.49969750567418	37.0	33.0	37.0	27.0	40.0
20	33.251594357035785	35.0	32.0	37.0	25.0	39.0
21	34.03358265031271	35.0	33.0	38.0	27.0	40.0
22	34.58260792496599	35.0	33.0	38.0	28.0	40.0
23	34.71990952144242	35.0	33.0	39.0	29.0	40.0
24	34.40824017664127	35.0	33.0	38.0	27.0	40.0
25	33.47049620629728	35.0	33.0	38.0	24.0	40.0
26	33.43781525881569	35.0	32.0	38.0	24.0	40.0
27	34.120689455857026	35.0	33.0	38.0	26.0	40.0
28	33.608329576238205	35.0	33.0	38.0	24.0	40.0
29	33.61398641280264	35.0	33.0	39.0	24.0	40.0
30	32.983611358372926	35.0	32.0	38.0	21.0	40.0
31	33.12349860698473	35.0	33.0	38.0	21.0	40.0
32	32.379986821266314	35.0	31.0	38.0	17.0	40.0
33	31.548354777676305	35.0	30.0	39.0	12.0	40.0
34	30.9083808268692	35.0	27.0	39.0	9.0	40.0
35	30.389377632374984	35.0	24.0	39.0	8.0	40.0
36	29.848575579267	35.0	23.0	39.0	7.0	40.0
37	29.46434613057736	35.0	21.0	39.0	7.0	40.0
38	29.441210131440528	35.0	21.0	39.0	7.0	40.0
39	28.981438023344083	35.0	20.0	38.0	7.0	40.0
40	28.698334161820977	35.0	19.0	38.0	7.0	40.0
41	28.33023517488796	34.0	18.0	38.0	7.0	40.0
42	28.444215807544246	34.0	18.0	38.0	7.0	40.0
43	28.406317314621074	35.0	18.0	38.0	7.0	40.0
44	28.48316243367282	35.0	18.0	39.0	7.0	40.0
45	28.66924461194024	35.0	18.0	39.0	7.0	40.0
46	28.376611215795982	35.0	18.0	39.0	7.0	40.0
47	28.14717408644787	34.0	18.0	38.0	7.0	40.0
48	28.16941223618449	34.0	18.0	38.0	7.0	40.0
49	28.370175986189302	34.0	18.0	39.0	7.0	40.0
50	28.222986486017827	34.0	18.0	39.0	7.0	40.0
51	27.098416625242283	33.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	11.0
10	15.0
11	15.0
12	24.0
13	27.0
14	52.0
15	55.0
16	131.0
17	303.0
18	657.0
19	1128.0
20	1856.0
21	2619.0
22	3664.0
23	5281.0
24	7702.0
25	11033.0
26	14228.0
27	15013.0
28	13635.0
29	12668.0
30	12814.0
31	13899.0
32	15808.0
33	18372.0
34	20437.0
35	22945.0
36	23661.0
37	22362.0
38	16623.0
39	2466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.02232677864737	2.13210331818935	25.070036106647553	38.775533796515724
2	38.06380510887869	24.792974424779104	26.558231121078652	10.584989345263555
3	9.731069057335198	25.18833643534521	54.26671136646514	10.81388314085446
4	8.736113198386183	2.2388433541803945	76.7198825474261	12.305160900007321
5	26.777491339414045	2.597597771175566	55.43738367455464	15.187527214855749
6	10.78344103672705	21.440874882952034	59.01144083634864	8.76424324397227
7	42.944946032700216	1.041582372865681	53.246322863561566	2.7671487308725324
8	40.608610876694065	17.733489011941785	37.84993969380638	3.807960417557773
9	38.482287704858024	2.4353683301927873	36.26039944664733	22.82194451830187
10	28.856031968062762	14.688893256110577	42.90332897895641	13.551745796870243
11	25.328601320185427	13.52862521145702	48.1921628922311	12.950610576126454
12	17.67029274514564	11.638902697016288	49.921968024230374	20.768836533607697
13	16.967041605493453	14.01839627912712	59.271547422247394	9.74301469313203
14	14.347479278175324	22.49170548998301	45.98376164217811	17.177053589663558
15	9.26364788889788	13.338651067978374	62.154684423276265	15.243016619847483
16	10.381142850536976	16.179785672173217	47.610680169088546	25.828391308201258
17	10.829682207553496	17.71190979888944	55.51676435114003	15.941643642417025
18	11.094798253625115	15.485012080505879	53.02860401758706	20.39158564828195
19	11.251247548254588	19.305688820040924	48.75322243159197	20.68984120011252
20	14.786385057936332	16.535457344446627	57.43500225425707	11.24315534335996
21	14.201434246981801	25.564816634490516	48.14515103522421	12.088598083303468
22	11.396521893267671	13.29240989715193	53.61817894562424	21.692889263956165
23	12.848109314127834	24.12748690796851	49.86416656069731	13.160237217206339
24	15.305056857372962	15.832206204794439	47.71664951889915	21.146087418933448
25	10.01814965954938	28.44294417534652	46.28086116473803	15.258045000366078
26	12.54985376229726	14.475413184128488	53.2166514456146	19.758081607959646
27	15.820645912087826	19.090282032607732	46.778724437302756	18.31034761800169
28	10.472469162919205	18.58085846733639	54.386167724433456	16.560504645310953
29	20.05710784597066	14.171762829034831	48.31161925019941	17.45951007479509
30	11.585340007475656	22.664339194401737	52.598561128901125	13.15175966922149
31	17.054129143883255	16.173234839639473	42.91912804565545	23.853507970821823
32	19.67793024519381	25.878485909929903	42.282926603701604	12.16065724117468
33	14.431098728753145	20.767680504337036	40.056414228408265	24.744806538501553
34	23.900905170918925	23.216535842687534	37.97402016885734	14.908538817536193
35	17.83676096012084	20.920276368064307	42.464808542285624	18.778154129529227
36	23.499377670909293	20.387346874289523	34.47972902673896	21.633546428062225
37	15.243401962937703	26.099672843716405	44.30405111190749	14.352874081438408
38	17.86142291789495	25.890816888816957	33.43853199696349	22.809228196324597
39	19.316478426567095	30.742671737781734	34.57567945620383	15.36517037944734
40	20.767295161246814	24.733631588885164	38.0110131055185	16.48806014434952
41	21.25359814110493	28.023305550096527	32.705224096274115	18.017872212524424
42	20.329545410756467	22.062047944387288	40.24870043042823	17.359706214428016
43	24.875823189176483	21.39232165358427	32.82699251278376	20.90486264445549
44	20.12955234693209	22.18882582106979	37.69580245771823	19.985819374279888
45	16.657996447136707	21.019694885341163	35.88969939385532	26.432609273666806
46	25.22147594110416	25.681960933917512	31.73801293982097	17.358550185157355
47	14.565583467240057	21.51794350099611	45.76719882547426	18.14927420628957
48	20.10874382006019	24.441156183407898	32.264776944152224	23.185323052379687
49	17.905352030180072	18.629026353613938	42.19660975149224	21.269011864713747
50	20.876732598869406	18.55619650956229	37.721235101672775	22.84583578989553
51	19.368885086837064	18.664092574823997	35.002254257077794	26.964768081261152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	352.0
2	693.0
3	8621.5
4	16550.0
5	12737.5
6	8925.0
7	8880.0
8	8835.0
9	8730.5
10	8626.0
11	8199.0
12	7772.0
13	7182.5
14	6593.0
15	6072.0
16	5551.0
17	5094.5
18	4638.0
19	4170.0
20	3702.0
21	3425.0
22	3148.0
23	2961.0
24	2774.0
25	2601.5
26	2619.0
27	2809.0
28	2822.5
29	2836.0
30	2860.5
31	2885.0
32	3297.5
33	3710.0
34	4007.0
35	4304.0
36	4531.5
37	4759.0
38	5012.0
39	5265.0
40	5824.5
41	6384.0
42	7238.0
43	8092.0
44	9071.0
45	10050.0
46	31561.0
47	53072.0
48	35698.5
49	18325.0
50	16307.5
51	14290.0
52	11774.0
53	9258.0
54	8329.0
55	7400.0
56	6942.0
57	6484.0
58	6027.0
59	5570.0
60	4915.0
61	4260.0
62	3769.0
63	3278.0
64	2732.0
65	2186.0
66	1830.0
67	1474.0
68	1166.5
69	859.0
70	758.5
71	658.0
72	529.5
73	401.0
74	363.5
75	242.5
76	159.0
77	122.0
78	85.0
79	70.0
80	55.0
81	33.5
82	12.0
83	10.5
84	9.0
85	6.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	259509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.53784506738769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.04015085640704	34.06806614856917
2	5.563635855670332	4.510756166570538
3	1.9805463450260379	2.4086124265033955
4	1.3072130451494617	2.1196639957734784
5	1.0132274364770737	2.053702841896701
6	0.8613272178730674	2.0949809586277497
7	0.7374224063791224	2.092546068131195
8	0.6504903337075639	2.1095581092536437
9	0.5665560806294018	2.067026635668699
>10	3.164520459676047	20.4401915923729
>50	0.06295067538527148	1.729610758499874
>100	0.03896946571469187	2.7062129923975777
>500	0.0029976512088224516	0.768400845169616
>1k	0.005995302417644903	5.000073818014624
>5k	0.0019984341392149676	6.829734660202898
>10k+	0.0019984341392149676	9.000861982347939
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	11792	4.543965719878694	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	10429	4.0187430879083195	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	8886	3.4241586996982765	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7975	3.073111144507512	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT	2429	0.9359983661452974	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	2256	0.8693340115371722	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC	2138	0.8238635268911675	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	1877	0.723288980343649	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	1846	0.7113433445468172	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	1798	0.6928468762162392	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGT	839	0.3233028526948969	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT	536	0.20654389635812245	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	522	0.20114909309503717	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG	485	0.18689139875688318	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCAAAGG	335	0.12908993522382653	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	296	0.1140615547052318	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTCAAAGGT	273	0.10519866363016311	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	262	0.10095988963773897	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10596934981060387	0.0
2	0.0	0.0	0.0	0.9298328767017714	0.0
3	0.0	0.0	0.0	1.029251393978629	0.0
4	0.0	0.0	0.0	1.8943466315233768	0.0
5	0.0	0.0	0.0	5.846810707913791	0.0
6	0.0	0.0	0.0	6.150075719917228	0.0
7	0.0	0.0	0.0	7.042530316867623	0.0
8	0.0	0.0	0.0	8.060606761229861	0.0
9	0.0	0.0	0.0	8.202027675340739	0.0
10	0.0	0.0	0.0	13.642301423072032	0.0
11	0.0	0.0	0.0	13.886223599181532	0.0
12	0.0	0.0	0.0	18.79356785313804	0.0
13	0.0	0.0	0.0	19.14037663433638	0.0
14	0.0	0.0	0.0	19.43516409835497	0.0
15	0.0	0.0	0.0	20.53840136565591	0.0
16	0.0	0.0	0.0	21.02316297315315	0.0
17	0.0	0.0	0.0	21.244735250029866	0.0
18	0.0	0.0	0.0	21.40542331865176	0.0
19	0.0	0.0	0.0	22.724067373385893	0.0
20	0.0	0.0	0.0	22.9965049381717	0.0
21	0.0	0.0	0.0	23.132145705929275	0.0
22	0.0	0.0	0.0	23.503231101811497	0.0
23	0.0	0.0	0.0	23.63848652647885	0.0
24	0.0	0.0	0.0	23.76218165843959	0.0
25	0.0	0.0	0.0	23.860444146445786	0.0
26	0.0	0.0	0.0	23.990690110940275	0.0
27	0.0	0.0	0.0	24.210721015456112	0.0
28	0.0	0.0	0.0	24.31938776689826	0.0
29	0.0	0.0	0.0	24.42304505816754	0.0
30	0.0	0.0	0.0	24.54596950394784	0.0
31	0.0	0.0	0.0	24.651553510668222	0.0
32	0.0	0.0	0.0	24.787194278425797	0.0
33	0.0	0.0	0.0	24.895475686777722	0.0
34	0.0	0.0	0.0	25.09392737824122	0.0
35	0.0	0.0	0.0	25.22725608745747	0.0
36	0.0	0.0	0.0	25.35210724868887	0.0
37	0.0	0.0	0.0	25.49391350588997	0.0
38	0.0	0.0	0.0	25.624159470384456	0.0
39	0.0	0.0	0.0	25.776369991021507	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1589858E-6	45.000004	6
GATACGC	20	7.023182E-4	45.000004	5
GGGTAAC	20	7.023182E-4	45.000004	7
GGCCGGT	20	7.023182E-4	45.000004	27
CGACCCT	20	7.023182E-4	45.000004	12
TCGCTTG	40	6.7793735E-9	45.000004	1
CGCAGGC	20	7.023182E-4	45.000004	34
ACGTCCA	20	7.023182E-4	45.000004	29
TGTCGTG	20	7.023182E-4	45.000004	1
CACGCGT	25	3.8824335E-5	45.0	41
CGGTGAA	75	0.0	45.0	19
TCCGTCC	25	3.8824335E-5	45.0	29
CGTTTTT	7130	0.0	44.337307	1
TGATACC	1325	0.0	43.4717	4
GATACCT	1315	0.0	43.288975	5
GCGACTG	275	0.0	42.545456	11
ACCGGTG	90	0.0	42.5	17
CCGGTGA	85	0.0	42.35294	18
ATACCTG	1350	0.0	42.166668	6
ACACGCG	75	0.0	42.0	36
>>END_MODULE